Mutagenetix > Incidental Mutation

Incidental Mutation 'R1136:Vmn2r68'

94999

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r68

Ensembl Gene

ENSMUSG00000096861

Gene Name

vomeronasal 2, receptor 68

Synonyms

EG620697, Vmn2r68-ps

MMRRC Submission

039209-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.077) question?

Stock #

R1136 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

84870726-84886912 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 84871549 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Valine at position 578 (D578V)

Ref Sequence

ENSEMBL: ENSMUSP00000129411 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000061074]

AlphaFold

L7N2B3

Predicted Effect

possibly damaging
Transcript: ENSMUST00000061074
AA Change: D578V

PolyPhen 2 Score 0.808 (Sensitivity: 0.84; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000129411
Gene: ENSMUSG00000096861
AA Change: D578V

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	77	463	4.5e-28	PFAM
Pfam:NCD3G	507	559	1.1e-18	PFAM
Pfam:7tm_3	589	827	3.7e-53	PFAM

Meta Mutation Damage Score

0.1795

Coding Region Coverage

1x: 98.9%
3x: 98.1%
10x: 95.7%
20x: 90.5%

Validation Efficiency

100% (52/52)

Allele List at MGI

Other mutations in this stock

Total: 48 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adcy2	C	T	13: 68,878,436 (GRCm39)	G401S	probably damaging	Het
Akap8l	C	T	17: 32,551,457 (GRCm39)	R511H	probably damaging	Het
Bmp2	T	C	2: 133,402,847 (GRCm39)	F133L	probably damaging	Het
C1qtnf3	A	G	15: 10,978,670 (GRCm39)	E290G	probably damaging	Het
Ccdc180	C	A	4: 45,914,589 (GRCm39)	D701E	probably benign	Het
Chmp7	C	T	14: 69,956,899 (GRCm39)	M336I	probably benign	Het
Csmd3	A	T	15: 47,539,213 (GRCm39)	I1508N	probably damaging	Het
Dgkh	T	C	14: 78,862,329 (GRCm39)	R80G	probably damaging	Het
Dock1	T	A	7: 134,449,902 (GRCm39)	V805D	possibly damaging	Het
Eef2	C	CN	10: 81,014,603 (GRCm39)		probably null	Het
Ercc6l2	T	A	13: 64,016,934 (GRCm39)	V679D	possibly damaging	Het
Esp6	T	C	17: 40,876,284 (GRCm39)	Y111H	probably benign	Het
Focad	T	C	4: 88,244,417 (GRCm39)	F799S	unknown	Het
Foxred1	C	A	9: 35,116,333 (GRCm39)	M438I	probably benign	Het
Galnt11	T	G	5: 25,463,943 (GRCm39)	V405G	probably damaging	Het
Gm4847	A	G	1: 166,457,935 (GRCm39)	Y473H	probably damaging	Het
Gpbp1l1	C	T	4: 116,450,115 (GRCm39)	T461M	probably damaging	Het
Hnrnpu	A	T	1: 178,158,790 (GRCm39)		probably benign	Het
Kmt2d	A	T	15: 98,755,646 (GRCm39)		probably benign	Het
Matr3	A	G	18: 35,705,948 (GRCm39)	H291R	probably damaging	Het
Mfsd14b	C	T	13: 65,243,506 (GRCm39)	S46N	probably benign	Het
Mtch1	C	T	17: 29,552,744 (GRCm39)		probably null	Het
Muc6	G	T	7: 141,218,685 (GRCm39)	T1996N	possibly damaging	Het
Mylk	T	C	16: 34,820,688 (GRCm39)	I1880T	probably damaging	Het
N4bp2	T	C	5: 65,965,815 (GRCm39)	L1288P	probably damaging	Het
Ncf2	A	T	1: 152,706,123 (GRCm39)	H245L	probably damaging	Het
Nmd3	T	A	3: 69,654,049 (GRCm39)		probably benign	Het
Npdc1	G	T	2: 25,297,727 (GRCm39)	A127S	probably benign	Het
Nudt3	C	A	17: 27,842,080 (GRCm39)	R27L	probably benign	Het
Nwd1	C	T	8: 73,424,397 (GRCm39)		probably benign	Het
Pex7	T	A	10: 19,764,434 (GRCm39)	I170F	probably benign	Het
Phyhipl	A	G	10: 70,404,902 (GRCm39)	V57A	probably damaging	Het
Pkhd1	G	A	1: 20,593,053 (GRCm39)	P1687S	possibly damaging	Het
Plekhj1	A	T	10: 80,633,654 (GRCm39)		probably null	Het
Prss21	T	A	17: 24,091,968 (GRCm39)	L312H	probably damaging	Het
Samsn1	T	C	16: 75,670,408 (GRCm39)	I232V	probably null	Het
Sec63	A	T	10: 42,682,542 (GRCm39)	D411V	probably damaging	Het
Slc44a4	C	T	17: 35,146,998 (GRCm39)	H343Y	probably damaging	Het
Sucla2	C	T	14: 73,798,074 (GRCm39)		probably benign	Het
Tedc2	T	A	17: 24,435,291 (GRCm39)	E366V	probably damaging	Het
Tedc2	C	A	17: 24,435,292 (GRCm39)	E366*	probably null	Het
Tent2	C	T	13: 93,312,205 (GRCm39)		probably null	Het
Tmtc3	A	G	10: 100,307,905 (GRCm39)		probably benign	Het
Trafd1	C	T	5: 121,511,387 (GRCm39)	R477H	possibly damaging	Het
Uhrf2	T	A	19: 30,033,626 (GRCm39)		probably benign	Het
Wdcp	G	A	12: 4,901,655 (GRCm39)	V504I	possibly damaging	Het
Wdr93	T	C	7: 79,423,196 (GRCm39)	Y487H	probably damaging	Het
Zfp457	T	C	13: 67,441,846 (GRCm39)	H147R	probably damaging	Het

Other mutations in Vmn2r68

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01391:Vmn2r68	APN	7	84,886,819 (GRCm39)	missense	probably benign
IGL01477:Vmn2r68	APN	7	84,882,691 (GRCm39)	missense	probably damaging	1.00
IGL01600:Vmn2r68	APN	7	84,871,468 (GRCm39)	missense	probably benign	0.39
IGL01979:Vmn2r68	APN	7	84,871,325 (GRCm39)	missense	probably benign
IGL01999:Vmn2r68	APN	7	84,871,439 (GRCm39)	missense	probably damaging	1.00
IGL02269:Vmn2r68	APN	7	84,870,947 (GRCm39)	missense	possibly damaging	0.84
IGL02517:Vmn2r68	APN	7	84,871,153 (GRCm39)	nonsense	probably null
IGL02827:Vmn2r68	APN	7	84,886,800 (GRCm39)	missense	probably damaging	1.00
IGL02852:Vmn2r68	APN	7	84,882,595 (GRCm39)	missense	probably damaging	1.00
IGL02982:Vmn2r68	APN	7	84,883,649 (GRCm39)	missense	probably benign	0.12
IGL03099:Vmn2r68	APN	7	84,871,448 (GRCm39)	nonsense	probably null
IGL03166:Vmn2r68	APN	7	84,871,331 (GRCm39)	missense	probably benign	0.01
IGL03168:Vmn2r68	APN	7	84,870,972 (GRCm39)	missense	probably damaging	1.00
IGL03243:Vmn2r68	APN	7	84,882,963 (GRCm39)	missense	possibly damaging	0.66
F5770:Vmn2r68	UTSW	7	84,871,088 (GRCm39)	missense	probably benign	0.01
R0280:Vmn2r68	UTSW	7	84,882,466 (GRCm39)	critical splice donor site	probably null
R0280:Vmn2r68	UTSW	7	84,882,457 (GRCm39)	splice site	probably benign
R0281:Vmn2r68	UTSW	7	84,882,466 (GRCm39)	critical splice donor site	probably null
R0281:Vmn2r68	UTSW	7	84,882,457 (GRCm39)	splice site	probably benign
R0348:Vmn2r68	UTSW	7	84,870,884 (GRCm39)	missense	possibly damaging	0.50
R0390:Vmn2r68	UTSW	7	84,882,466 (GRCm39)	critical splice donor site	probably null
R0390:Vmn2r68	UTSW	7	84,882,457 (GRCm39)	splice site	probably benign
R0722:Vmn2r68	UTSW	7	84,870,794 (GRCm39)	missense	possibly damaging	0.95
R1129:Vmn2r68	UTSW	7	84,886,712 (GRCm39)	splice site	probably null
R1319:Vmn2r68	UTSW	7	84,881,700 (GRCm39)	missense	probably damaging	0.96
R1614:Vmn2r68	UTSW	7	84,870,946 (GRCm39)	missense	possibly damaging	0.93
R1682:Vmn2r68	UTSW	7	84,882,574 (GRCm39)	missense	possibly damaging	0.68
R1837:Vmn2r68	UTSW	7	84,882,886 (GRCm39)	missense	probably damaging	0.96
R1893:Vmn2r68	UTSW	7	84,883,867 (GRCm39)	nonsense	probably null
R1908:Vmn2r68	UTSW	7	84,883,260 (GRCm39)	missense	probably benign	0.09
R1909:Vmn2r68	UTSW	7	84,883,260 (GRCm39)	missense	probably benign	0.09
R1951:Vmn2r68	UTSW	7	84,883,102 (GRCm39)	missense	probably damaging	1.00
R2177:Vmn2r68	UTSW	7	84,871,123 (GRCm39)	missense	probably benign	0.01
R2178:Vmn2r68	UTSW	7	84,870,758 (GRCm39)	frame shift	probably null
R2185:Vmn2r68	UTSW	7	84,882,901 (GRCm39)	nonsense	probably null
R2188:Vmn2r68	UTSW	7	84,870,758 (GRCm39)	frame shift	probably null
R2282:Vmn2r68	UTSW	7	84,870,859 (GRCm39)	missense	possibly damaging	0.65
R2567:Vmn2r68	UTSW	7	84,883,803 (GRCm39)	missense	probably benign
R2869:Vmn2r68	UTSW	7	84,882,834 (GRCm39)	missense	probably benign	0.25
R2869:Vmn2r68	UTSW	7	84,882,834 (GRCm39)	missense	probably benign	0.25
R2870:Vmn2r68	UTSW	7	84,882,834 (GRCm39)	missense	probably benign	0.25
R2870:Vmn2r68	UTSW	7	84,882,834 (GRCm39)	missense	probably benign	0.25
R2871:Vmn2r68	UTSW	7	84,882,834 (GRCm39)	missense	probably benign	0.25
R2871:Vmn2r68	UTSW	7	84,882,834 (GRCm39)	missense	probably benign	0.25
R2873:Vmn2r68	UTSW	7	84,882,834 (GRCm39)	missense	probably benign	0.25
R2874:Vmn2r68	UTSW	7	84,882,834 (GRCm39)	missense	probably benign	0.25
R3149:Vmn2r68	UTSW	7	84,886,875 (GRCm39)	missense	probably benign	0.00
R3401:Vmn2r68	UTSW	7	84,870,758 (GRCm39)	frame shift	probably null
R3978:Vmn2r68	UTSW	7	84,881,670 (GRCm39)	missense	probably benign	0.00
R4399:Vmn2r68	UTSW	7	84,870,758 (GRCm39)	frame shift	probably null
R4401:Vmn2r68	UTSW	7	84,870,758 (GRCm39)	frame shift	probably null
R4421:Vmn2r68	UTSW	7	84,870,758 (GRCm39)	frame shift	probably null
R4478:Vmn2r68	UTSW	7	84,870,758 (GRCm39)	frame shift	probably null
R4479:Vmn2r68	UTSW	7	84,870,758 (GRCm39)	frame shift	probably null
R4495:Vmn2r68	UTSW	7	84,870,758 (GRCm39)	frame shift	probably null
R4628:Vmn2r68	UTSW	7	84,883,673 (GRCm39)	missense	probably benign	0.00
R4649:Vmn2r68	UTSW	7	84,870,743 (GRCm39)	missense	probably benign
R4654:Vmn2r68	UTSW	7	84,882,769 (GRCm39)	nonsense	probably null
R4793:Vmn2r68	UTSW	7	84,883,648 (GRCm39)	missense	probably benign	0.01
R5007:Vmn2r68	UTSW	7	84,881,622 (GRCm39)	missense	probably benign
R5021:Vmn2r68	UTSW	7	84,882,942 (GRCm39)	missense	possibly damaging	0.62
R5082:Vmn2r68	UTSW	7	84,883,076 (GRCm39)	missense	probably benign	0.12
R5177:Vmn2r68	UTSW	7	84,871,199 (GRCm39)	missense	probably damaging	0.99
R5221:Vmn2r68	UTSW	7	84,871,085 (GRCm39)	missense	probably damaging	1.00
R5514:Vmn2r68	UTSW	7	84,886,767 (GRCm39)	missense	possibly damaging	0.92
R5521:Vmn2r68	UTSW	7	84,882,926 (GRCm39)	missense	probably benign	0.03
R5563:Vmn2r68	UTSW	7	84,871,283 (GRCm39)	missense	probably damaging	1.00
R5664:Vmn2r68	UTSW	7	84,882,978 (GRCm39)	missense	probably benign	0.02
R5829:Vmn2r68	UTSW	7	84,886,812 (GRCm39)	missense	probably benign	0.00
R6016:Vmn2r68	UTSW	7	84,871,453 (GRCm39)	missense	probably damaging	0.99
R6356:Vmn2r68	UTSW	7	84,883,048 (GRCm39)	missense	possibly damaging	0.85
R6413:Vmn2r68	UTSW	7	84,870,973 (GRCm39)	missense	probably damaging	1.00
R6418:Vmn2r68	UTSW	7	84,882,915 (GRCm39)	missense	probably benign
R6699:Vmn2r68	UTSW	7	84,881,583 (GRCm39)	missense	possibly damaging	0.58
R7287:Vmn2r68	UTSW	7	84,871,460 (GRCm39)	missense	probably benign	0.33
R7319:Vmn2r68	UTSW	7	84,883,042 (GRCm39)	missense	probably benign
R7374:Vmn2r68	UTSW	7	84,881,607 (GRCm39)	missense	possibly damaging	0.66
R7585:Vmn2r68	UTSW	7	84,881,587 (GRCm39)	missense	probably damaging	1.00
R7605:Vmn2r68	UTSW	7	84,883,116 (GRCm39)	missense	probably benign	0.01
R7892:Vmn2r68	UTSW	7	84,883,722 (GRCm39)	missense	probably benign
R7979:Vmn2r68	UTSW	7	84,883,625 (GRCm39)	critical splice donor site	probably null
R8177:Vmn2r68	UTSW	7	84,871,422 (GRCm39)	nonsense	probably null
R8349:Vmn2r68	UTSW	7	84,882,785 (GRCm39)	missense	probably damaging	1.00
R8378:Vmn2r68	UTSW	7	84,871,108 (GRCm39)	missense	probably benign	0.00
R8397:Vmn2r68	UTSW	7	84,886,722 (GRCm39)	missense	possibly damaging	0.71
R8449:Vmn2r68	UTSW	7	84,882,785 (GRCm39)	missense	probably damaging	1.00
R8543:Vmn2r68	UTSW	7	84,883,648 (GRCm39)	missense	probably benign	0.01
R8680:Vmn2r68	UTSW	7	84,871,321 (GRCm39)	missense	possibly damaging	0.68
R9056:Vmn2r68	UTSW	7	84,871,420 (GRCm39)	missense	possibly damaging	0.71
R9342:Vmn2r68	UTSW	7	84,882,993 (GRCm39)	missense	probably benign	0.39
R9734:Vmn2r68	UTSW	7	84,882,757 (GRCm39)	missense	possibly damaging	0.54
V7581:Vmn2r68	UTSW	7	84,871,088 (GRCm39)	missense	probably benign	0.01
Z1176:Vmn2r68	UTSW	7	84,871,289 (GRCm39)	missense	probably benign	0.27
Z1176:Vmn2r68	UTSW	7	84,870,941 (GRCm39)	missense	probably damaging	1.00
Z1176:Vmn2r68	UTSW	7	84,871,307 (GRCm39)	missense	possibly damaging	0.92

Predicted Primers

PCR Primer
(F):5'- CTTAGCCAAGATAGTAGAGGTAGCCACA -3'
(R):5'- GCAAATCCTGCATAAAGATGACAAGCAT -3'

Sequencing Primer
(F):5'- CAATCACAAAAGTGGTCTGCTG -3'
(R):5'- GTATCAAATGCTATGCAGATTCCTC -3'

Posted On

2014-01-05