Mutagenetix > Incidental Mutation

Incidental Mutation 'R1018:Zfp358'

96604

Institutional Source

Beutler Lab

Gene Symbol

Zfp358

Ensembl Gene

ENSMUSG00000047264

Gene Name

zinc finger protein 358

Synonyms

Zfend

MMRRC Submission

039122-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.155) question?

Stock #

R1018 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

3543138-3547208 bp(+) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

C to A at 3546843 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Serine to Stop codon at position 475 (S475*)

Ref Sequence

ENSEMBL: ENSMUSP00000146953 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000004683] [ENSMUST00000061508] [ENSMUST00000160338] [ENSMUST00000207318] [ENSMUST00000208306] [ENSMUST00000208423]

AlphaFold

E9Q8M1

Predicted Effect

probably benign
Transcript: ENSMUST00000004683

SMART Domains

Protein: ENSMUSP00000004683
Gene: ENSMUSG00000004567

Domain	Start	End	E-Value	Type
low complexity region	30	39	N/A	INTRINSIC
transmembrane domain	70	92	N/A	INTRINSIC
transmembrane domain	299	321	N/A	INTRINSIC
transmembrane domain	348	370	N/A	INTRINSIC
Pfam:PKD_channel	378	524	2.1e-12	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000061508
AA Change: S475*

SMART Domains

Protein: ENSMUSP00000060344
Gene: ENSMUSG00000047264
AA Change: S475*

Domain	Start	End	E-Value	Type
low complexity region	24	49	N/A	INTRINSIC
ZnF_C2H2	154	176	1.47e-3	SMART
ZnF_C2H2	182	204	2.95e-3	SMART
ZnF_C2H2	210	232	1.67e-2	SMART
ZnF_C2H2	238	260	3.63e-3	SMART
ZnF_C2H2	266	288	2.4e-3	SMART
ZnF_C2H2	294	316	1.4e-4	SMART
ZnF_C2H2	322	344	4.01e-5	SMART
ZnF_C2H2	350	372	2.4e-3	SMART
ZnF_C2H2	378	400	1.84e-4	SMART
low complexity region	422	430	N/A	INTRINSIC
low complexity region	447	473	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000160338

SMART Domains

Protein: ENSMUSP00000123717
Gene: ENSMUSG00000004567

Domain	Start	End	E-Value	Type
low complexity region	30	39	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000207318

Predicted Effect

probably benign
Transcript: ENSMUST00000208306

Predicted Effect

probably null
Transcript: ENSMUST00000208423
AA Change: S475*

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000208739

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 99.1%
3x: 98.2%
10x: 95.9%
20x: 91.7%

Validation Efficiency

100% (50/50)

Allele List at MGI

Other mutations in this stock

Total: 47 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca7	G	T	10: 79,837,325 (GRCm39)	C403F	probably damaging	Het
Adam20	C	T	8: 41,249,146 (GRCm39)	Q419*	probably null	Het
Ankrd36	C	T	11: 5,596,876 (GRCm39)		probably benign	Het
Arl8b	T	A	6: 108,795,572 (GRCm39)	I170K	probably damaging	Het
Atp5pb	A	T	3: 105,861,488 (GRCm39)	V78E	possibly damaging	Het
Car9	G	T	4: 43,512,439 (GRCm39)		probably null	Het
Cgnl1	A	G	9: 71,633,340 (GRCm39)	Y4H	probably damaging	Het
Cnn2	T	C	10: 79,829,397 (GRCm39)	C176R	probably damaging	Het
Dst	A	G	1: 34,233,174 (GRCm39)	D3392G	probably damaging	Het
Eed	T	C	7: 89,617,019 (GRCm39)		probably benign	Het
Efl1	T	A	7: 82,412,221 (GRCm39)	V870E	possibly damaging	Het
Epx	T	C	11: 87,760,129 (GRCm39)	N495S	probably benign	Het
Fbxw20	T	A	9: 109,050,404 (GRCm39)	Y407F	probably benign	Het
Gbp9	T	A	5: 105,228,126 (GRCm39)	Q552L	probably benign	Het
Hspa13	C	A	16: 75,558,164 (GRCm39)	V134L	possibly damaging	Het
Il16	T	C	7: 83,323,746 (GRCm39)	N268S	probably damaging	Het
Kif14	G	A	1: 136,423,579 (GRCm39)		probably benign	Het
Lrig1	G	A	6: 94,599,583 (GRCm39)		probably benign	Het
Myo7a	T	A	7: 97,756,212 (GRCm39)	D29V	probably damaging	Het
Nsd2	A	G	5: 34,000,585 (GRCm39)	K34R	probably damaging	Het
Or4a15	C	A	2: 89,193,523 (GRCm39)	L83F	possibly damaging	Het
P3h1	C	A	4: 119,095,104 (GRCm39)	T287K	probably damaging	Het
Pkhd1	A	T	1: 20,271,483 (GRCm39)	H3023Q	possibly damaging	Het
Plxna1	A	G	6: 89,319,942 (GRCm39)	L535P	probably damaging	Het
Prom1	A	T	5: 44,187,056 (GRCm39)	S400R	probably benign	Het
Psap	T	G	10: 60,136,590 (GRCm39)	L523R	probably damaging	Het
Psme4	C	T	11: 30,754,310 (GRCm39)	T189I	probably damaging	Het
Ptpn12	A	T	5: 21,234,867 (GRCm39)	S39T	possibly damaging	Het
Qtrt2	T	A	16: 43,698,363 (GRCm39)	H98L	possibly damaging	Het
Rad54l2	C	A	9: 106,589,589 (GRCm39)	C601F	probably benign	Het
Saxo4	A	T	19: 10,456,824 (GRCm39)		probably benign	Het
Sfswap	A	G	5: 129,631,640 (GRCm39)	K756R	possibly damaging	Het
Slc24a5	T	C	2: 124,910,827 (GRCm39)	V86A	probably damaging	Het
Srrm2	T	C	17: 24,041,514 (GRCm39)	S2575P	probably damaging	Het
Stam	T	C	2: 14,122,185 (GRCm39)		probably benign	Het
Tbx6	T	A	7: 126,382,364 (GRCm39)		probably benign	Het
Tmem131	A	C	1: 36,833,900 (GRCm39)	F1727V	probably damaging	Het
Tpr	T	A	1: 150,317,934 (GRCm39)	H2147Q	possibly damaging	Het
Trio	T	A	15: 27,871,257 (GRCm39)	H620L	probably damaging	Het
Uba5	T	C	9: 103,927,102 (GRCm39)	T292A	probably benign	Het
Unc5a	T	A	13: 55,138,765 (GRCm39)	V48E	possibly damaging	Het
Upf1	G	T	8: 70,791,556 (GRCm39)	H514Q	possibly damaging	Het
Usp9y	A	G	Y: 1,341,414 (GRCm39)		probably benign	Het
Vmn2r6	T	A	3: 64,464,261 (GRCm39)	D191V	probably benign	Het
Wapl	T	C	14: 34,413,863 (GRCm39)	Y242H	possibly damaging	Het
Zfp341	A	T	2: 154,487,972 (GRCm39)	N812Y	probably damaging	Het
Zfp957	C	A	14: 79,450,182 (GRCm39)	C539F	probably damaging	Het

Other mutations in Zfp358

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01405:Zfp358	APN	8	3,545,663 (GRCm39)	missense	probably benign	0.19
IGL02301:Zfp358	APN	8	3,546,858 (GRCm39)	missense	probably benign
IGL02510:Zfp358	APN	8	3,546,786 (GRCm39)	missense	probably benign	0.04
IGL02860:Zfp358	APN	8	3,546,074 (GRCm39)	missense	probably damaging	1.00
R1960:Zfp358	UTSW	8	3,545,742 (GRCm39)	missense	possibly damaging	0.46
R2047:Zfp358	UTSW	8	3,545,934 (GRCm39)	missense	probably damaging	1.00
R2202:Zfp358	UTSW	8	3,546,995 (GRCm39)	missense	possibly damaging	0.80
R2354:Zfp358	UTSW	8	3,545,454 (GRCm39)	missense	possibly damaging	0.95
R4688:Zfp358	UTSW	8	3,545,493 (GRCm39)	missense	probably damaging	1.00
R4689:Zfp358	UTSW	8	3,546,146 (GRCm39)	splice site	probably null
R4876:Zfp358	UTSW	8	3,546,170 (GRCm39)	missense	probably damaging	0.98
R5830:Zfp358	UTSW	8	3,545,846 (GRCm39)	missense	probably benign	0.36
R6589:Zfp358	UTSW	8	3,545,907 (GRCm39)	missense	probably damaging	0.99
R6834:Zfp358	UTSW	8	3,545,613 (GRCm39)	missense	probably benign	0.19
R9043:Zfp358	UTSW	8	3,545,810 (GRCm39)	missense	probably benign	0.01
R9357:Zfp358	UTSW	8	3,545,568 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GGCAGAGTTCAGCACTCCTACAAC -3'
(R):5'- TTTATCCATCAGGCCCCAGCAATG -3'

Sequencing Primer
(F):5'- TCAGCTCTGTGGCAAAGC -3'
(R):5'- GGGTCAGTttgggacttgg -3'

Posted On

2014-01-05