Incidental Mutation 'R1109:Zfp746'
ID98637
Institutional Source Beutler Lab
Gene Symbol Zfp746
Ensembl Gene ENSMUSG00000057691
Gene Namezinc finger protein 746
Synonyms2810407L07Rik
MMRRC Submission 039182-MU
Accession Numbers
Is this an essential gene? Probably non essential (E-score: 0.214) question?
Stock #R1109 (G1)
Quality Score225
Status Validated
Chromosome6
Chromosomal Location48062395-48086593 bp(-) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) A to G at 48064922 bp
ZygosityHeterozygous
Amino Acid Change Valine to Alanine at position 289 (V289A)
Ref Sequence ENSEMBL: ENSMUSP00000145506 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000073124] [ENSMUST00000203609]
Predicted Effect possibly damaging
Transcript: ENSMUST00000073124
AA Change: V290A

PolyPhen 2 Score 0.942 (Sensitivity: 0.80; Specificity: 0.94)
SMART Domains Protein: ENSMUSP00000072868
Gene: ENSMUSG00000057691
AA Change: V290A

DomainStartEndE-ValueType
Pfam:DUF3669 11 81 3.4e-12 PFAM
KRAB 107 156 1.37e-12 SMART
low complexity region 168 183 N/A INTRINSIC
low complexity region 190 206 N/A INTRINSIC
ZnF_C2H2 454 474 3.65e1 SMART
low complexity region 481 511 N/A INTRINSIC
ZnF_C2H2 517 539 1.92e-2 SMART
ZnF_C2H2 545 567 1.82e-3 SMART
ZnF_C2H2 573 595 1.72e-4 SMART
low complexity region 601 623 N/A INTRINSIC
Predicted Effect unknown
Transcript: ENSMUST00000203033
AA Change: V73A
Predicted Effect possibly damaging
Transcript: ENSMUST00000203609
AA Change: V289A

PolyPhen 2 Score 0.942 (Sensitivity: 0.80; Specificity: 0.94)
SMART Domains Protein: ENSMUSP00000145506
Gene: ENSMUSG00000057691
AA Change: V289A

DomainStartEndE-ValueType
Pfam:DUF3669 11 81 4.6e-9 PFAM
KRAB 107 156 5.7e-15 SMART
low complexity region 168 183 N/A INTRINSIC
low complexity region 190 206 N/A INTRINSIC
ZnF_C2H2 453 473 1.6e-1 SMART
low complexity region 480 510 N/A INTRINSIC
ZnF_C2H2 516 538 8e-5 SMART
ZnF_C2H2 544 566 7.8e-6 SMART
ZnF_C2H2 572 594 7.3e-7 SMART
low complexity region 600 622 N/A INTRINSIC
Predicted Effect noncoding transcript
Transcript: ENSMUST00000204771
Meta Mutation Damage Score 0.114 question?
Coding Region Coverage
  • 1x: 98.9%
  • 3x: 98.0%
  • 10x: 95.3%
  • 20x: 88.9%
Validation Efficiency 100% (80/80)
Allele List at MGI
Other mutations in this stock
Total: 78 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
9030624J02Rik A T 7: 118,775,329 I351F probably damaging Het
Abcd3 T G 3: 121,779,596 E262D probably damaging Het
Abcg1 G A 17: 31,111,236 A504T probably benign Het
Acot11 T C 4: 106,749,348 T515A probably benign Het
Alg8 A T 7: 97,383,684 probably null Het
Arhgap44 T A 11: 65,026,816 H375L probably benign Het
Aspm A T 1: 139,456,758 I98F probably damaging Het
Atp8b5 T C 4: 43,305,719 probably benign Het
Ccdc129 G T 6: 55,968,260 K655N probably damaging Het
Coa3 T C 11: 101,278,785 K48R probably damaging Het
Col12a1 A G 9: 79,699,723 S473P probably damaging Het
Dnmt1 A T 9: 20,922,388 Y451N probably damaging Het
Dock5 A G 14: 67,806,478 Y819H possibly damaging Het
Dtx1 G A 5: 120,710,419 probably benign Het
Dus2 T A 8: 106,053,482 F479I probably benign Het
Esrp1 T C 4: 11,365,205 E262G probably damaging Het
Exoc4 A G 6: 33,442,016 Y466C probably damaging Het
Fasn A G 11: 120,812,324 F1625S possibly damaging Het
Fbxw25 A T 9: 109,650,060 H374Q probably benign Het
Focad T C 4: 88,196,747 probably benign Het
Gad2 G T 2: 22,681,394 R448L probably damaging Het
Gad2 G A 2: 22,690,159 probably benign Het
Galnt16 G T 12: 80,590,631 E377D probably benign Het
Gcc1 A C 6: 28,419,167 L389R probably damaging Het
Ggct A T 6: 54,989,569 probably benign Het
Gm7052 G A 17: 22,040,152 probably benign Het
Gps2 T A 11: 69,915,681 H177Q possibly damaging Het
Heg1 T A 16: 33,763,591 L1256Q probably damaging Het
Ikbkap T C 4: 56,786,723 T407A probably benign Het
Il1f6 G A 2: 24,216,590 G62E probably damaging Het
Il3ra C T 14: 14,349,317 R138W probably damaging Het
Kdm4b T A 17: 56,399,430 I848N probably damaging Het
L3hypdh A G 12: 72,073,996 V327A possibly damaging Het
Lepr G T 4: 101,771,355 L552F probably damaging Het
Lpin3 T C 2: 160,899,021 I449T probably damaging Het
Lrrn1 A G 6: 107,567,264 K8E probably benign Het
Mex3a G T 3: 88,536,660 D348Y possibly damaging Het
Mindy2 G A 9: 70,631,079 R325* probably null Het
Mkrn1 A T 6: 39,399,334 M382K probably damaging Het
Mroh1 G T 15: 76,446,509 probably benign Het
Myo15 T A 11: 60,493,066 D1646E probably damaging Het
Neu2 A G 1: 87,596,728 D145G probably damaging Het
Olfr317 T C 11: 58,732,916 Y83C probably benign Het
Olfr718-ps1 A T 5: 143,137,619 N216K probably benign Het
Olfr743 T C 14: 50,533,702 S97P possibly damaging Het
Plekhm2 T A 4: 141,627,984 I938F probably benign Het
Pnmal1 A T 7: 16,961,467 K416* probably null Het
Ppid T C 3: 79,598,861 S198P probably benign Het
Rabl6 G T 2: 25,587,526 P304Q probably damaging Het
Rasal2 A T 1: 157,177,638 probably benign Het
Ripor1 G T 8: 105,618,928 probably benign Het
Rnf216 A T 5: 143,068,369 L658Q probably damaging Het
Rnf219 A T 14: 104,479,764 L391* probably null Het
Safb T A 17: 56,601,228 probably benign Het
Sf3b5 T C 10: 13,008,753 M44T probably benign Het
Slc26a9 A T 1: 131,758,798 M419L probably benign Het
Slc38a8 T C 8: 119,482,655 D393G probably benign Het
Slc6a3 A G 13: 73,557,080 D230G probably benign Het
Smc1b T C 15: 85,112,815 T535A probably damaging Het
Smg7 G C 1: 152,845,583 P626R probably damaging Het
Smu1 C T 4: 40,755,722 V48M probably benign Het
Spag17 T C 3: 100,027,351 Y650H possibly damaging Het
Spata1 C T 3: 146,475,298 V302I possibly damaging Het
Sptb G A 12: 76,603,603 A1780V probably damaging Het
Srrm2 G A 17: 23,819,617 probably benign Het
Sspo A C 6: 48,497,443 N4933H probably damaging Het
Sult2a2 T C 7: 13,734,873 I88T probably benign Het
Tgfa G T 6: 86,270,090 probably benign Het
Thsd1 T A 8: 22,243,692 C252S possibly damaging Het
Tmem102 T A 11: 69,804,804 H114L probably damaging Het
Tnni3k T A 3: 154,792,777 K808N possibly damaging Het
Trpm7 G T 2: 126,797,793 L1628M probably benign Het
Ttn A G 2: 76,730,737 Y29107H probably damaging Het
Upb1 T C 10: 75,438,165 L342P probably damaging Het
Vmn2r120 T C 17: 57,525,829 T117A probably benign Het
Vmn2r16 A G 5: 109,339,786 D175G probably damaging Het
Zfhx4 T G 3: 5,399,870 M1696R possibly damaging Het
Zfyve26 A C 12: 79,272,127 F1146V probably damaging Het
Other mutations in Zfp746
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01993:Zfp746 APN 6 48082158 missense probably damaging 0.99
R0020:Zfp746 UTSW 6 48064707 missense probably benign 0.05
R0020:Zfp746 UTSW 6 48064707 missense probably benign 0.05
R0734:Zfp746 UTSW 6 48064899 missense probably damaging 0.97
R1656:Zfp746 UTSW 6 48064477 missense probably damaging 0.99
R1657:Zfp746 UTSW 6 48082174 missense possibly damaging 0.94
R2474:Zfp746 UTSW 6 48064769 missense probably damaging 1.00
R4748:Zfp746 UTSW 6 48064556 missense probably benign 0.05
R5168:Zfp746 UTSW 6 48064395 missense possibly damaging 0.88
R5386:Zfp746 UTSW 6 48064176 missense possibly damaging 0.94
R5446:Zfp746 UTSW 6 48064173 missense probably damaging 0.97
R5560:Zfp746 UTSW 6 48082174 missense possibly damaging 0.94
R7251:Zfp746 UTSW 6 48064877 missense probably damaging 1.00
R7524:Zfp746 UTSW 6 48064889 missense possibly damaging 0.95
Predicted Primers PCR Primer
(F):5'- GGTTTACACCGGAAATTCCAGCCC -3'
(R):5'- AGGTCCCAATGTGGTGCTCCATAG -3'

Sequencing Primer
(F):5'- ATGTCCCTCTCTGGCCGAG -3'
(R):5'- GTGCTCCATAGGGCTGAG -3'
Posted On2014-01-05