|Institutional Source||Beutler Lab|
|Gene Name||doublecortin-like kinase 3|
|Synonyms||Click-I, -II related, Dcamkl3|
|Is this an essential gene?||Possibly non essential (E-score: 0.366)|
|Stock #||R1024 (G1)|
|Chromosomal Location||111439081-111489118 bp(+) (GRCm38)|
|Type of Mutation||missense|
|DNA Base Change (assembly)||A to G at 111469070 bp|
|Amino Acid Change||Isoleucine to Valine at position 561 (I561V)|
|Ref Sequence||ENSEMBL: ENSMUSP00000107510 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000111879]|
|Predicted Effect||possibly damaging
AA Change: I561V
PolyPhen 2 Score 0.952 (Sensitivity: 0.79; Specificity: 0.95)
AA Change: I561V
|Meta Mutation Damage Score||0.048|
|Coding Region Coverage||
|Validation Efficiency||90% (36/40)|
|MGI Phenotype||FUNCTION: This gene encodes a member of the protein kinase superfamily and the doublecortin family. Differently from the other two closely related family members (DCLK1 and DCLK2), the protein encoded by this gene contains only one N-terminal doublecortin domain and is unable to bind microtubules and to regulate microtubule polymerization. The protein contains a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmoduline-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. [provided by RefSeq, Sep 2010]|
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Dclk3||
(F):5'- AAGGGCATCATCTCAGCGGATGTG -3'
(R):5'- ACGTCCCGGTGGACGATATTCTTG -3'
(F):5'- CATCTCAGCGGATGTGGAGAAG -3'
(R):5'- TGCATGTGGACGAGGGC -3'