Mutagenetix > Incidental Mutation

Incidental Mutation 'R1216:Gpr152'

99697

Institutional Source

Beutler Lab

Gene Symbol

Gpr152

Ensembl Gene

ENSMUSG00000044724

Gene Name

G protein-coupled receptor 152

Synonyms

LOC269053, A930009H15Rik

MMRRC Submission

039285-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R1216 (G1)

Quality Score

192

Status

Validated

Chromosome

Chromosomal Location

4189798-4195740 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 4193554 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Aspartic acid at position 365 (V365D)

Ref Sequence

ENSEMBL: ENSMUSP00000094062 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000025761] [ENSMUST00000096338]

AlphaFold

Q8BXS7

Predicted Effect

probably benign
Transcript: ENSMUST00000025761

SMART Domains

Protein: ENSMUSP00000025761
Gene: ENSMUSG00000024842

Domain	Start	End	E-Value	Type
low complexity region	45	67	N/A	INTRINSIC
low complexity region	79	97	N/A	INTRINSIC
EFh	129	157	1.08e-6	SMART
Blast:EFh	165	193	2e-7	BLAST
EFh	206	234	1.05e-4	SMART
EFh	243	271	1.55e-7	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000096338
AA Change: V365D

PolyPhen 2 Score 0.861 (Sensitivity: 0.83; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000094062
Gene: ENSMUSG00000044724
AA Change: V365D

Domain	Start	End	E-Value	Type
Pfam:7tm_1	47	295	7e-19	PFAM
low complexity region	347	361	N/A	INTRINSIC
low complexity region	419	433	N/A	INTRINSIC

Meta Mutation Damage Score

0.1795

Coding Region Coverage

1x: 99.1%
3x: 98.2%
10x: 96.1%
20x: 92.3%

Validation Efficiency

98% (45/46)

Allele List at MGI

Other mutations in this stock

Total: 45 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A830018L16Rik	A	T	1: 11,868,716 (GRCm39)	D332V	probably damaging	Het
Akr1c12	T	C	13: 4,326,322 (GRCm39)	Y53C	probably benign	Het
Arhgap23	T	C	11: 97,383,498 (GRCm39)		probably benign	Het
AU040320	T	C	4: 126,710,276 (GRCm39)		probably benign	Het
B4galnt2	A	G	11: 95,782,767 (GRCm39)	L15P	probably benign	Het
Cadps2	A	G	6: 23,583,472 (GRCm39)		probably benign	Het
Cul9	C	G	17: 46,833,101 (GRCm39)	A1326P	probably damaging	Het
Dppa4	T	C	16: 48,113,343 (GRCm39)	F244S	possibly damaging	Het
Exoc1	A	G	5: 76,702,035 (GRCm39)	K445R	probably benign	Het
Fam47e	A	G	5: 92,710,343 (GRCm39)	E114G	probably damaging	Het
Fgf12	A	T	16: 27,981,202 (GRCm39)	N171K	possibly damaging	Het
Fyb2	G	A	4: 104,852,903 (GRCm39)	V528M	possibly damaging	Het
Ghr	A	G	15: 3,349,337 (GRCm39)	S614P	probably damaging	Het
Gm10985	A	C	3: 53,752,674 (GRCm39)	Y19S	probably damaging	Het
Guca1a	A	G	17: 47,706,637 (GRCm39)		probably benign	Het
Hivep1	G	A	13: 42,310,997 (GRCm39)	G1079D	probably benign	Het
Hnrnpm	T	C	17: 33,868,687 (GRCm39)	D580G	probably damaging	Het
Ints6	A	T	14: 62,945,147 (GRCm39)	D394E	probably damaging	Het
Kat6b	T	A	14: 21,672,108 (GRCm39)	Y339*	probably null	Het
Kcnj11	C	A	7: 45,749,285 (GRCm39)	V13L	probably benign	Het
Lama3	C	T	18: 12,554,191 (GRCm39)		probably benign	Het
Mtrex	A	G	13: 113,050,876 (GRCm39)		probably benign	Het
Myo18a	A	G	11: 77,709,473 (GRCm39)	T161A	probably benign	Het
Ncapg	G	T	5: 45,857,261 (GRCm39)	S991I	possibly damaging	Het
Nrde2	G	A	12: 100,116,069 (GRCm39)		probably benign	Het
Or4c127	T	A	2: 89,832,822 (GRCm39)	I24N	probably benign	Het
Or5d18	T	C	2: 87,864,602 (GRCm39)	R294G	probably damaging	Het
Pcdhb7	A	T	18: 37,476,927 (GRCm39)	T688S	probably damaging	Het
Pla2g6	T	C	15: 79,190,635 (GRCm39)	D309G	probably benign	Het
Plod1	A	T	4: 148,005,584 (GRCm39)	V404D	probably damaging	Het
Ppp2r2a	G	T	14: 67,266,447 (GRCm39)	Y71*	probably null	Het
Prcp	A	G	7: 92,566,954 (GRCm39)	N222S	probably benign	Het
Rad21	T	C	15: 51,833,532 (GRCm39)	T316A	possibly damaging	Het
Ranbp2	T	C	10: 58,319,034 (GRCm39)		probably benign	Het
Rapgef4	C	A	2: 72,038,492 (GRCm39)	P548T	possibly damaging	Het
Ric1	G	T	19: 29,555,135 (GRCm39)	M416I	probably benign	Het
Slc9a8	T	C	2: 167,266,041 (GRCm39)	F6S	probably benign	Het
Smpdl3a	T	G	10: 57,678,575 (GRCm39)	I126S	probably null	Het
Sphkap	A	T	1: 83,268,698 (GRCm39)	L98Q	probably damaging	Het
Spink4	T	G	4: 40,924,974 (GRCm39)		probably benign	Het
Taar5	A	T	10: 23,847,605 (GRCm39)	L334F	probably damaging	Het
Tecta	A	T	9: 42,289,203 (GRCm39)	I454K	probably benign	Het
Ttc7	C	T	17: 87,654,006 (GRCm39)	T561M	possibly damaging	Het
Vmn2r9	G	T	5: 108,995,440 (GRCm39)	H403N	probably damaging	Het
Zdbf2	G	A	1: 63,342,161 (GRCm39)	C180Y	possibly damaging	Het

Other mutations in Gpr152

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00493:Gpr152	APN	19	4,193,506 (GRCm39)	missense	probably benign
IGL01400:Gpr152	APN	19	4,193,626 (GRCm39)	missense	probably benign	0.33
IGL01538:Gpr152	APN	19	4,192,951 (GRCm39)	missense	probably damaging	1.00
IGL02288:Gpr152	APN	19	4,193,694 (GRCm39)	missense	probably benign
IGL02513:Gpr152	APN	19	4,192,843 (GRCm39)	missense	probably damaging	1.00
IGL03335:Gpr152	APN	19	4,193,770 (GRCm39)	missense	possibly damaging	0.69
R0318:Gpr152	UTSW	19	4,193,541 (GRCm39)	missense	possibly damaging	0.73
R1936:Gpr152	UTSW	19	4,192,531 (GRCm39)	missense	probably damaging	1.00
R2248:Gpr152	UTSW	19	4,193,805 (GRCm39)	missense	probably benign	0.00
R3161:Gpr152	UTSW	19	4,192,713 (GRCm39)	missense	probably benign	0.00
R4193:Gpr152	UTSW	19	4,192,906 (GRCm39)	missense	probably damaging	1.00
R4719:Gpr152	UTSW	19	4,193,223 (GRCm39)	missense	possibly damaging	0.92
R4852:Gpr152	UTSW	19	4,193,790 (GRCm39)	missense	probably benign	0.00
R5014:Gpr152	UTSW	19	4,193,506 (GRCm39)	missense	probably benign	0.00
R5381:Gpr152	UTSW	19	4,192,516 (GRCm39)	missense	probably damaging	1.00
R5431:Gpr152	UTSW	19	4,193,746 (GRCm39)	missense	probably benign	0.21
R5470:Gpr152	UTSW	19	4,193,128 (GRCm39)	missense	probably damaging	1.00
R7331:Gpr152	UTSW	19	4,192,608 (GRCm39)	missense	probably damaging	0.99
R7350:Gpr152	UTSW	19	4,192,963 (GRCm39)	missense	possibly damaging	0.89
R7806:Gpr152	UTSW	19	4,193,487 (GRCm39)	missense	probably benign
R8315:Gpr152	UTSW	19	4,193,469 (GRCm39)	missense	probably damaging	1.00
R8889:Gpr152	UTSW	19	4,192,723 (GRCm39)	missense	probably damaging	1.00
R9455:Gpr152	UTSW	19	4,193,844 (GRCm39)	missense	probably benign	0.01
R9651:Gpr152	UTSW	19	4,192,614 (GRCm39)	missense	probably damaging	1.00
R9709:Gpr152	UTSW	19	4,192,640 (GRCm39)	missense	probably benign	0.00
R9761:Gpr152	UTSW	19	4,193,227 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AGCCACTGCTTGTAGGACCTGTTG -3'
(R):5'- AGGCTGGACCACAGACTTTGATTG -3'

Sequencing Primer
(F):5'- GGCCAAGACCATTTTGTCAG -3'
(R):5'- ATTGTGGTTGGGCTGAGG -3'

Posted On

2014-01-15