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|Gene Name||paired box 5|
|Chromosomal Location||44,531,145-44,710,487 bp (-)|
|MGI Phenotype||Null mutants exhibit impaired development of the midbrain resulting in a reduced inferior colliculus and an altered cerebellar folial pattern, failure of B cell differentiation, runting, and high postnatal mortality with few survivors.|
|Amino Acid Change|
|Institutional Source||Beutler Lab|
Ensembl: ENSMUSP00000014174 (fasta)
|Gene Model||not available|
|Phenotypic Category||decrease in B cells, immune system, T-dependent humoral response defect- decreased antibody response to rSFV, T-independent B cell response defect- decreased TNP-specific IgM to TNP-Ficoll immunization|
|Alleles Listed at MGI|
|Mode of Inheritance||Unknown|
|Local Stock||Live Mice, Sperm|
|Last Updated||12/09/2016 11:49 AM by Katherine Timer|
|Record Created||09/14/2010 5:55 PM by Carrie N. Arnold|
|Other Mutations in This Stock||
Stock #: K2124 Run Code: SLD00303
Coding Region Coverage: 1x: 88.1% 3x: 81.9%
Validation Efficiency: 194/202
The Apple mutation was identified in a T-independent B cell response screen of N-ethyl-N-nitrosourea (ENU)-mutagenized G3 mice whereby homozygous Apple mutants had a diminshed T-independent antibody response to 4-hydroxy-3-nitrophenylacetyl-Ficoll (NP-Ficoll) (Figure 1). Homozygotes also had a diminished T-dependent antibody response to ovalbumin administered with aluminum hydroxide (Figure 2A) and recombinant Semliki Forest virus (rSFV)-encoded β-galactosidase (rSFV-β-gal), another T-dependent antigen (Figure 2B). The Apple mice exhibited a global reduction in antibody subtypes, with the exception of IgE and IgG3 (Figure 3). Fluorescence activated cell sorting (FACS) of peripheral blood from homozygous Apple mice determined that the Apple mice have a significant depletion of B cells with a corresponding increase in the percentage of T cells (including CD4+ and CD8+), macrophages, and monocytes (Figure 4).
|Nature of Mutation|
Whole genome sequencing of a homozygous Apple mouse using the SOLiD technique identified an A to T transversion at base pair 44,724,449 on Chromosome 4 in the GenBank genomic region NC_00070 encoding Pax5 (NCBI Ref37) (Figure 5). This mutation is 175 nt 5’ to the ATG.
The mutated nucleotide is indicated in red lettering and the start codon (ATG) is highlighted. This mutation results in loss of Pax5 protein expression (Figure 6).
Pax5 encodes Pax5 [alternatively, B-cell lineage-specific activator protein (BSAP)], a member of the paired box (PAX) family of transcription factors that have roles in cellular differentiation, migration, and proliferation (1-5). Pax5 has been further classified as a member of a Pax family subgroup that includes Pax2 and Pax8. The Pax5/2/8 subgroup share a highly conserved paired box DNA binding domain (PRD), an octapeptide motif, a partial homeodomain, a transactivation domain, and a repressor domain (1;6-12) (Figure 7). Pax5 recognizes target genes via the PRD, a 128 amino acid (aa) domain that has two helix-turn-helix motifs separated by a polypeptide linker (4;6;9;12-16). At the N-terminus, a β-hairpin binds to the DNA phosphate backbone, followed by a β-turn that contacts the minor groove of DNA targets (8;12;15-17). The N-terminus is also essential for the recombination function of Pax5 (see the “VH-to-DJH” subsection in the “Background”, below) (8). The polypeptide linker also contributes to DNA binding by contacting the minor groove (12). The centrally located octapeptide motif (TN8TCCT; N can be any combination of eight nucleotides) is a characteristic feature of most Pax proteins and is a region rich in proline, serine, threonine, and tyrosine residues [(18-20); reviewed in (21)]. The octapeptide motif is required for Pax5-Groucho-mediated gene repression; mutation or deletion of this sequence leads to an increase in the transactivation of Pax proteins (18-20;22). Also, the octapeptide motif has the potential to direct cytosine methylation to surrounding gene sequences (23). The partial homeodomain of Pax5 is not required for transcriptional activation of Pax5 target genes, but is required for Pax5-mediated activation of recombination, as deletion of this domain resulted in impaired RAG-mediated recombination (see the “VH-to-DJH” subsection in the “Background”, below) (8). Although the homeodomain is not required for activation of target genes, it is required for interaction with the TATA box binding protein, retinoblastoma protein, and the death domain-associated protein Daxx (24;25) as well as for the recruitment of the Groucho corepressor (18), and the cooperation of Pax5 and Ets-1 (17;26). The 55 amino acid proline-, serine-, and threonine-rich transactivation domain and repressor sequences are at the C-terminus of Pax5 (10;18). The transactivation domain is active in both promoter and enhancer positions during Pax5-mediated regulation of gene expression and can be negatively regulated by the adjacent repressor sequences at the extreme C-terminus (10;18).
E1A-binding protein (p300), a histone acetyltransferase that regulates transcription through chromatin remodeling, interacts with the Pax5 C-terminus, and can subsequently facilitate the acetylation of several lysines in the Pax5 N-terminal PRD (e.g., Lys67, Lys87, Lys94, Lys98, and Lys103) upon p300 overexpression (7). Mutations of Lys67, Lys87, Lys89, or Lys103 altered the induction of Pax5 target genes (e.g., CD19 and Blnk (B cell linker; see the record for busy)) in murine pro B cells [see Table 2 for the functional significance of these Pax5 targets; (7)], indicating that Pax5 activity is regulated through acetylation.
Pax5 generates multiple isoforms from two known alternative promoters to generate transcripts with distinct first exons (exon 1A and 1B) and otherwise similar in-frame sequences [(9;11;27-29); reviewed in (30)]. Alternative splicing also occurs throughout the transcript to generate multiple protein isoforms [(9); reviewed in (30)]. In both murine spleen and B-lymphoid cell lines, Pax5 generates four isoforms by alternative splicing that are expressed at detectable levels in normal B cells: Pax-5a (i.e., full-length Pax5), Pax5b, Pax5d, and Pax5e (28;31) (Figure 7). Pax-5d lacks exons 6-10 and Pax-5e lacks exons 2 and 6-10 (31). As a result, Pax5a and Pax5d, but not Pax5e, possess an intact DNA-binding domain; Pax5d and Pax5e do not have transactivation, repression, or partial homeodomain homology regions, but do contain a novel 128 base pair sequence with an unknown function (28;31). In a transient transfection system, the Pax5d and Pax5e isoforms worked to either compete or enhance the transactivating function of Pax5a: Pax5d had a transactivating function opposite of Pax5a, while isoform Pax5e increased the activity of Pax5a (31). Lowen et al. also showed that the ratio of Pax5d to Pax5e correlated with the proliferation state of the lipopolysaccharide-activated B cell: increased levels of Pax-5e led to increased proliferation, while increased Pax5d levels inhibited cell growth (31). The function of Pax-5b was not addressed. Some studies have indicated that the differential expression of PAX5 isoforms can be disease-specific in B cell malignancies (11;32;33). However, the findings have been inconsistent as isoform expression patterns differed between the studies, even in normal cells (11;32;33). One study showed that there were no significant differences in PAX5 transcript patterns between normal and cancerous B-lymphocytes (11). In contrast, another study showed a higher number or leukemia-specific isoforms in B-cell precursor acute lymphoblastic leukemia (BCP-ALL) (33). Robichaud et al. identified the expression of five PAX5 isoforms that were generated through alternative splicing in the 3’ region in healthy B-lymphocytes, B cell lines, and primary lymphoma samples (9). The isoforms excluded different combinations of exons 7-9 resulting in changes at the C-terminus of the protein (i.e., shorter protein variants) (9). In three of the isoforms, there was a shift in the reading frame that resulted in novel protein sequences that contained a higher ratio of proline, serine, and threonine residues (9). In this study, all of the PAX5 isoforms could recognize and bind the same DNA targets, but with different transactivation potentials (9). Furthermore, in individual lymphoma samples, Robichaud et al. noted one predominant isoform, either the full length PAX5 or PAX5Δ8, an isoform that lacks 34 amino acids within the transactivating domain; healthy samples expressed multiple isoforms (9). This study also determined that upon activation of distinct cellular pathways with mitogenic agents, different isoform expression patterns were induced (9). In another study, on ‘aged’ normal lymphocytes, researchers found that transcripts in these lymphocytes corresponded to isoforms already described (9;11), including those with various exon deletions (e.g., Δ2, Δ7/8, Δ2/7/8, Δ2/7/8/9, Δ2/4/8, and Δ2/3/4/8) (32). Nebral et al. found that there was a time-dependent accumulation of splice variants, indicating that the conflicting finds in other studies may be due to unintentional ‘ageing’ of lymphocytes or cold shock conditions (32).
Pax5 interacts with partner proteins to increase Pax5 specificity for target genes [Table 1; (16)]. Pax5 can form ternary complexes between Pax5, Ets proto-oncogene family proteins, and DNA [Figure 8; (26)]. The crystal structure of the ternary complex of the Pax5 PRD (aa 19-142) and the Ets domain (aa 335-436) of Ets-1 bound to a 25 base pair Mb-1 DNA promoter fragment has been solved at a resolution of 2.25 Å [Figure 8; (17); PDB: 1K78]. Pax5 binds DNA similar to Pax6 [PDB: 6PAX; (14)] in that the N-terminal domain (NTD) and C-terminal domain (CTD) of the Pax5 PRD each have three α helices with a helix-turn-helix motif that contact the DNA major groove (17). Furthermore, the NTD has a short β hairpin at its N-terminus, followed by a β turn; the linker that connects the NTD and CTD binds in the DNA minor groove in an extended conformation (17). The Pax5 PRD has several direct contacts with the DNA bases as well as contacts with the sugar phosphate backbone (17). For example, in helix C (i.e., the recognition helix), His62 directly contacts G9 (and indirectly contacts T10 and A11 through water-mediated interactions) and Lys67 directly contacts T11 (17). Previous studies had determined that His62 is essential for Pax5 binding, as mutations at this base results in 95% loss in Pax5 binding (16;26). Within the β turn, Asn29 and Gly30 contact the minor groove through direct and water-mediated interactions (17). Residues Ile83, Gly84, Gly85, and Ser86 within the linker domain contact the minor groove through main chain or side chain atoms (17). Within the CTD, Arg137 is in van der Waals contact with T21 and S133 is in a hydrogen bond with G23 in the major groove (17). Pax5 recruits Ets-1 to a Pax5-Ets binding site to subsequently enhance its DNA-binding ability at the promoter of the Mb-1 (alternatively, Ig-α; see the record for crab)) gene; Gln22 in Pax5 is an essential residue for this interaction [(7;12;16;17;26;34-36); reviewed in (30)]. The interaction of Pax5 with Ets-1 counteracts Ets-1 autoinhibition to subsequently increase the binding of Ets-1 to the Mb-1 promoter >1000-fold (34). In addition to Ets-1, Pax5 can recruit other members of the Ets family (i.e., Fli-1, GABPα (together with the ankyrin-repeat protein GABPβ), and PU.1) to the Mb-1 promoter in vitro (16;37-39); reviewed in (21)]. In the case of PU.1, the interaction with Pax5 can result in either a cooperative function (e.g., recruiting Groucho proteins such as Grg4 (7;24;38)) to the Igh locus (38)), or a repressive function (e.g., antagonizing activity at the Igk locus (37)).
Pax5 is expressed in the embryonic central nervous system (transiently in the mesencephalon (i.e., the midbrain), in the midbrain-hindbrain boundary preceding the development of the midbrain and cerebellum, and spinal cord) and fetal liver, B-lymphocytes, and adult testis [(3;4;43-45); reviewed in (21;30)]. In B-lymphocytes, Pax5 expression is initiated in pre-pro-B cells and expressed by the B220+ pro-B cell stage in the bone marrow (46;47). Expression persists to the mature B cell stage in peripheral lymphoid organs where it is subsequently repressed in terminally differentiated plasma cells [(7;44;48-51); reviewed in (21;52)].
Several factors regulate Pax5 expression. For example, in adult B lymphopoiesis, the transcription factors E2A and early B cell factor (EBF1) initiate Pax5 expression [(53;54); reviewed in (55)]. Also, Pax2 activates Pax5 expression via binding to an enhancer sequence within the Pax5 gene (45). Metastasis-associated protein 1 (MTA1) has also been identified as a regulator of Pax5 transcription [(56); reviewed in (30)]. The JAK2/STAT5 pathway regulates Pax5 activity and may also regulate its expression: phosphorylated STAT5 (see record for domino for information on STAT1) enhances activation of the Pax5 promoter in pre-B cells (57). Also, JAK2 activation can induce the DNA-binding ability and nuclear localization of Pax5 in breast carcinoma cells (58).
The Pax protein family regulates pattern formation, morphogenesis, cellular differentiation, and organogenesis by activating (or repressing) genes that encode secreted proteins, cell surface receptors, cell cycle regulators, and transcription factors [(3;16); reviewed in (21)]. Pax5/BSAP was identified as a regulator of B cell lineage development (7;48;49) that also plays a role in neural development (44;50) and cancer (1;11;48). In order to facilitate gene regulation, Pax5 can either induce active chromatin (to activate target genes) or eliminate active chromatin (to repress genes) (59). Pax5 induces chromatin changes by recruiting both histone-modifying complexes and chromatin-remodeling complexes to the target genes (48;59). ChIP profiling of histone modifications in Pax5-deficient cells determined that Pax5 induces H3K4 methylation and H3K9 acetylation at regulatory elements of target genes [(59;60); reviewed in (52)]. In the case of repressed genes, Pax5 eliminates active histone modifications (59).
Pax5 and B Cells
Pax5 plays a role in several aspects of B cell biology including the initiation of B cell lineage commitment in the bone marrow, maintenance of the B cell fate in more mature cells, V-DJ recombination of the Igh locus, and B cell migration [(25;44;48;61); reviewed in (62)].
Early B cell differentiation
Mutation of Pax5 leads to B cell developmental arrest at an early progenitor B cell stage in the bone marrow [(50); Figure 9]. During B cell differentiation from a common hematopoietic stem cell progenitor to a mature B cell, Pax5 and other lineage-specific B lymphoid transcription factors such as EBF and E2A function to both activate B lineage-specific genes as well as to repress the transcription of other lineage-inappropriate genes (1;9;18;21;47-50;52;60;61;63-67). Pax5 can bind to a large part of the cis-regulatory genome (i.e., ~8000 Pax5 target genes) in pro-B and mature B cells (47). Microarray analysis of both wild-type and Pax5-deficient pro-B cells found that Pax5 repressed 110 genes and activate 170 genes (46;60;61). The Pax5-regulated genes included those that encode regulatory and structural proteins that have diverse functions such as transcriptional control, adhesion, migration, immune function, cellular metabolism, and receptor signaling [Table 2; (47;59-61;68); reviewed in (52;55).
Mature B cell function, identity, and survival
In addition to its role in early B cell lymphopoiesis, Pax5 also functions in mature B cells to downregulate B cell-specific genes and reactivate lineage-inappropriate genes (e.g., X-box-binding protein 1 (Xbp1) (71;81), B lymphocyte-induced maturation protein 1 [Blimp-1; (68;81)], immunoglobulin J chain (Igj) (82), and the Igh 3′ enhancer (83-85)) that were previously repressed during B cell maturation to maintain B cell identity throughout B lymphopoiesis (i.e., to repress the plasma cell pathway [(47;60;68;78;86); reviewed in (21)]. Pax5 also functions to control the migration of committed B cells by activating genes that code for adhesion receptors as well as adaptors and effectors downstream of integrin signaling [(59;60); reviewed in (52)]. In a study with conditional knockout of Pax5 in mature B cells, loss of Pax5 caused dedifferentiation of mature B cells into uncommitted progenitors in the bone marrow and the subsequent conversion of those progenitors to functional T cells (78;87). Figure 10 shows an overall view of BCR signaling; factors controlled by Pax5 are bolded.
In Pax5−/− progenitors, VH-DJH recombination of the distal VHJ558 gene family is reduced by ∼50-fold [(50); reviewed in (52)]. Expression of Pax5 in developing T lineage cells in vitro can induce rearrangement of DH-proximal VH7183 genes to the DJH region (85;88). In situ hybridization determined that Pax5 mediates Igh locus contraction and subsequent distal VHJ558 gene recombination (83-85). Subsequent findings determined that Pax5 binds to the VH coding regions and subsequently facilitates RAG1-RAG2 (see the records for maladaptive and snowcock, respectively)-mediated VH-to-DJH recombination by physically interacting with RAG1-RAG2 (8). Zhang et al. propose two Pax5 functions in regulating Igh recombination (8). One, Pax5 binds to the Igh enhancer regions to establish the proper chromosomal configuration for VH-to-DJH rearrangements. Two, the binding of Pax5 to VH coding regions as well as to the RAG1-RAG2 complex mediates RAG-mediated recombination of VH genes (8).
Midbrain and cerebellum development
Studies have found that the Pax5-Pax2 complex is necessary for development of the midbrain and cerebellum (89). In a mouse model in which only one copy of both Pax2 (Pax2+/-) and Pax5 (Pax5+/-) were expressed, 20% of the compound heterozygotes had missing inferior colliculi and disrupted vermis of the cerebellum (89). A compound model with ablation of Pax5 expression (Pax5-/-) and Pax2 heterozygosity had complete loss of the posterior midbrain and cerebellum by embryonic (E) day 9.5 (89). In the chick brain, changes in Pax5 expression via transfection resulted in swelling of the anterior tectum and changed the diencephalon into a tectum-like structure (90). This study proposed a role of Pax5 in regulating the activity of the isthmus organizer (90).
Pax5 in cancer
Pax5 has been proposed to act as either an oncogene (11;48) or a tumor suppressor (1;87) in human cancers. PAX5 point mutations, monallelic loss of PAX5, and PAX5 fusions with other genes that cause increased Pax5 expression [e.g., PAX5 fusion to IGH (27;91-94)] have been identified in acute lymphoblastic leukemia (ALL) cell lines (i.e., REH and a B-lymphoid cell line derived from a patient) and in lymphoplasmocytoid lymphoma [(10;95); reviewed in (52)]. Overexpression or aberrant somatic hypermutations of PAX5 has also been identified in non-Hodgkin lymphoma [(27;96;97); reviewed in (30)]. In addition to B cell-associated cancers, PAX5 deregulation has been linked to increased malignancy in nonlymphoid cancers such as astrocytomas, medulloblastomas, neuroblastomas, oral carcinomas, colorectal carcinomas, cervical carcinomas, bladder carcinomas, breast cancer, and small-cell lung carcinomas by altering cancer proliferation, apoptosis, and phenotype transitioning [(42;58); reviewed in (30)]. A recent study on hepatocellular cancer (HCC) showed that ectopic expression of PAX5 suppressed HCC growth through the induction of p53-mediated apoptosis indicating that, in the case of hepatocarcinogenesis, PAX5 functions as a tumor suppressor (1).
Pax5 animal models
Pax5 knockout (Pax5-/-) mice exhibit growth retardation and early death by three weeks of age (44). The ~5% of Pax5-/- mice to survive to adulthood were fertile, but were runted (44). Pax5-/- mice are osteopenic, are missing ~60% of their bone mass, and their spleens produced ~5 times the amount of osteoclasts compared to controls (3). Examination of the brains of Pax5-/- mice determined that the inferior colliculus in the auditory midbrain region was underdeveloped near the midline by as early as embryonic day (E) 16.5, but hearing was not affected (44;98). In the Pax5-/- mice, B cell development is arrested at the pro-B cell stage resulting in a lack of immunoglobulin in the serum [(3;25;44;50;86); reviewed in (21)]. In the Pax5-/- fetal liver, B cell development halts before the appearance of B220+ progenitors (50); in the Pax5-/- bone marrow, development progresses to a c-Kit+B220+ progenitor cell stage [(44;50); reviewed in (52)]. Pro-B cells from Pax5-/- mice can differentiate into almost all hemopoietic cell lineages both in vitro and in vivo [Figure 11; (18;67;99;100)]. Retroviral transduction of Pax5 causes the Pax5-deficient pro-B cells to progress along the B-lymphoid lineage (18;67;99). DH-to-JH rearrangement proceeds normally in the Pax5-/- mice along with rearrangement of a subset of the VH7183 genes; rearrangement of DH-distal VHJ558 genes is severely compromised (8;101).
The Apple mutation causes a loss of Pax5 protein expression that subsequently causes defects in B cell maturation. Similar to previous studies using conditional knockout or Pax5 (78;87) and the Pax5-/- mice (18;67;99;100), the loss of Pax5 expression in Apple is proposed to cause a conversion of B cell progenitors to other lymphoid cells. Similar to the Pax5-/- mice, arrest of B cell development in the Apple mice leads to a reduction in the amount of immunoglobulin in the serum [(3;25;44;50;86); reviewed in (21)]. It is possible that the mutation with the 5’ untranslated region of Pax5 results in alteration or prevention of binding of a protein (e.g., Pax2, E2A, or EBF1) that regulates Pax5 expression. Loss of this association would subsequently result in reduced protein expression.
|Primers||Primers cannot be located by automatic search.|
Apple genotyping is performed by amplifying the region containing the mutation using PCR, followed by sequencing of the amplified region to detect the single nucleotide transition.
Apple_PCR_F: 5’- TCTCCTCAAGTGAGCACACAGATCC -3’
Apple_PCR_R: 5’- ATCATTTCACGGTGCCTTCGGACG -3’
Primer for sequencing
Apple Seq_R: 5’- CGGAAGGCTTCAATTATTCCGAC -3’
The following sequence of 714 nucleotides are amplified (Chr. 4: 44710046-44710759, GRCm38; NCBI RefSeq NC_000070):
tctcctcaag tgagcacaca gatccaaact tgaacatctc cagattaaca ccaacacaag tatcagaaac agaatcaaac aaaaccaaag aaaacaggcc tccttgaaga caccttgacg gactagtgaa aaccatgtga acgcactgta gaaggacagg ccttttcctg agaaaactac acacacacac acacacacac acacacacac acacaaacac acacaccttt cttcttgcat ggatattagc atggatacta gcactgggta gtgtcccaga gtaagcatgg atgaatcccg tttcgcggtg ttcctacctg tcctgatggt ccgaggagtc gggtaatttt tctctaaatc catttcgata tttcaggact tgatgaaatg gacagcgagg aaaagtttcc actttttttg gggtgtcttt tttccttttc tttctttttt cttttctttt ttctttcttt gttttatttt cttttctccc tctttttctt tcttcctttc ctctttcttt ctttctttct ttctttcttt ctttctttct ttctttcttt ctttctttct ttcttttttt tggtgctggg gcccgctcac aagtcggaat aattgaagcc ttccgctccc ccgccgagct ggggtagctg atcactgagc tgaaactaaa cgttttaggt ggaaaaaaag cgtccgaagg caccgtgaaa tgat
Primer binding sites are underlined; sequencing primer binding sites are highlighted in gray; the mutated nucleotide is indicated in red (T>A, Chr. + strand (shown); A>T, sense strand).
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|Science Writers||Anne Murray|
|Illustrators||Diantha La Vine|
|Authors||Carrie Arnold and Elaine Pirie|
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