Mutagenetix > Incidental Mutations

Incidental Mutation 'R1181:Trim66'

101580

Institutional Source

Beutler Lab

Gene Symbol

Trim66

Ensembl Gene

ENSMUSG00000031026

Gene Name

tripartite motif-containing 66

Synonyms

Tif1d, D7H11orf29

MMRRC Submission

039253-MU

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.138) question?

Stock #

R1181 (G1)

Quality Score

151

Status

Validated

Chromosome

Chromosomal Location

109449006-109508134 bp(-) (GRCm38)

Type of Mutation

critical splice donor site (1 bp from exon)

DNA Base Change (assembly)

C to T at 109484577 bp

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000102352 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000033339] [ENSMUST00000106739] [ENSMUST00000106741]

Predicted Effect

probably null
Transcript: ENSMUST00000033339

SMART Domains

Protein: ENSMUSP00000033339
Gene: ENSMUSG00000031026

Domain	Start	End	E-Value	Type
PHD	4	69	7.77e0	SMART
BBC	108	234	1.61e-39	SMART
low complexity region	318	333	N/A	INTRINSIC
low complexity region	452	486	N/A	INTRINSIC
low complexity region	517	530	N/A	INTRINSIC
low complexity region	568	581	N/A	INTRINSIC
PHD	998	1041	4.09e-10	SMART
BROMO	1069	1175	8.22e-27	SMART
low complexity region	1185	1199	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000106739

SMART Domains

Protein: ENSMUSP00000102350
Gene: ENSMUSG00000031026

Domain	Start	End	E-Value	Type
PHD	4	69	7.77e0	SMART
BBC	108	234	1.61e-39	SMART
low complexity region	318	333	N/A	INTRINSIC
low complexity region	452	486	N/A	INTRINSIC
low complexity region	517	530	N/A	INTRINSIC
low complexity region	568	581	N/A	INTRINSIC
PHD	998	1041	4.09e-10	SMART
BROMO	1069	1175	8.22e-27	SMART
low complexity region	1185	1199	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000106741

SMART Domains

Protein: ENSMUSP00000102352
Gene: ENSMUSG00000031026

Domain	Start	End	E-Value	Type
RING	28	78	2.38e-2	SMART
BBOX	102	140	1.48e0	SMART
PHD	106	171	7.77e0	SMART
RING	107	170	4.38e0	SMART
BBOX	162	203	4.21e-3	SMART
BBC	210	336	1.61e-39	SMART
low complexity region	420	435	N/A	INTRINSIC
low complexity region	554	588	N/A	INTRINSIC
low complexity region	619	632	N/A	INTRINSIC
low complexity region	670	683	N/A	INTRINSIC
PHD	1100	1143	4.09e-10	SMART
BROMO	1171	1277	8.22e-27	SMART
low complexity region	1287	1301	N/A	INTRINSIC

Meta Mutation Damage Score

0.9492

Coding Region Coverage

1x: 99.3%
3x: 97.9%
10x: 93.9%
20x: 84.2%

Validation Efficiency

100% (52/52)

Allele List at MGI

Other mutations in this stock

Total: 45 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Actn3	T	A	19: 4,872,610	Q64L	probably benign	Het
Ankrd12	T	C	17: 66,042,574	N88S	probably benign	Het
Apcdd1	A	T	18: 62,937,097	Y145F	probably damaging	Het
Bnipl	C	A	3: 95,245,649		probably null	Het
Bod1	T	C	11: 31,666,943		probably benign	Het
Cbarp	G	T	10: 80,135,494	H166N	probably damaging	Het
Cdr2l	T	A	11: 115,394,179	I447N	probably damaging	Het
Cped1	T	G	6: 22,215,562	I698M	probably damaging	Het
Ecm1	G	A	3: 95,735,350	H404Y	possibly damaging	Het
Ehbp1	C	T	11: 22,062,831	V902I	probably benign	Het
Eps8	T	C	6: 137,522,854	Q209R	possibly damaging	Het
Fastk	C	T	5: 24,441,731		probably null	Het
Gm6797	T	A	X: 8,641,765		noncoding transcript	Het
Gp1ba	C	T	11: 70,641,427	P673L	probably damaging	Het
Gstm1	A	G	3: 108,014,811	F170S	probably damaging	Het
Hgf	G	C	5: 16,618,925	G707R	probably damaging	Het
Klhl5	T	C	5: 65,162,885	M594T	probably damaging	Het
Kyat3	A	C	3: 142,737,770		probably null	Het
Mettl14	C	T	3: 123,374,002	G236S	probably damaging	Het
Nob1	G	A	8: 107,421,490	P107S	probably damaging	Het
Nupl1	A	T	14: 60,244,670		probably benign	Het
Olfr1155	G	A	2: 87,943,146	L161F	probably benign	Het
Olfr1463	C	A	19: 13,234,831	H194N	probably benign	Het
Olfr444	T	A	6: 42,955,558	L20Q	probably benign	Het
Olfr503	C	A	7: 108,545,302	T257N	probably benign	Het
Olfr676	T	A	7: 105,035,814	N205K	probably damaging	Het
Olfr808	A	G	10: 129,768,164	I223V	probably benign	Het
Pald1	A	G	10: 61,347,587		probably benign	Het
Pds5a	A	T	5: 65,627,202		probably null	Het
Pirb	A	T	7: 3,717,638	L287Q	probably benign	Het
Plekha2	A	C	8: 25,059,202	S189A	probably benign	Het
Prune2	T	C	19: 17,123,105	V1991A	probably benign	Het
Sec61g	A	C	11: 16,504,722		probably benign	Het
Serinc3	T	C	2: 163,625,526	K445R	probably damaging	Het
Shf	G	A	2: 122,368,682	P51S	probably damaging	Het
Slfn8	T	C	11: 83,016,745	E324G	probably benign	Het
Spink13	A	G	18: 62,608,170		probably benign	Het
Tas2r121	A	T	6: 132,700,169	I280N	probably damaging	Het
Tbc1d7	T	C	13: 43,153,139	I242M	probably damaging	Het
Tenm2	C	T	11: 36,063,177	G1236R	possibly damaging	Het
Tnni3k	A	T	3: 154,875,513	H600Q	probably damaging	Het
Ttn	A	T	2: 76,969,703	I387N	probably damaging	Het
Tulp3	A	T	6: 128,325,952	H301Q	possibly damaging	Het
Ubqln5	T	A	7: 104,128,741	Q292L	probably damaging	Het
Zfp454	T	C	11: 50,873,586	K229E	probably damaging	Het

Other mutations in Trim66

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01539:Trim66	APN	7	109455066	missense	probably benign	0.02
IGL01758:Trim66	APN	7	109486045	critical splice donor site	probably null
IGL01982:Trim66	APN	7	109458763	missense	probably benign	0.00
IGL01983:Trim66	APN	7	109458251	nonsense	probably null
IGL02149:Trim66	APN	7	109460902	missense	possibly damaging	0.66
IGL02392:Trim66	APN	7	109460274	missense	probably benign	0.01
IGL02483:Trim66	APN	7	109477630	splice site	probably benign
IGL02832:Trim66	APN	7	109460497	missense	probably damaging	1.00
IGL02945:Trim66	APN	7	109460176	nonsense	probably null
IGL03085:Trim66	APN	7	109458745	missense	probably benign	0.17
PIT1430001:Trim66	UTSW	7	109475247	missense	probably damaging	0.99
R0326:Trim66	UTSW	7	109460172	missense	probably benign	0.00
R0358:Trim66	UTSW	7	109460176	nonsense	probably null
R0401:Trim66	UTSW	7	109475264	missense	probably damaging	0.98
R0470:Trim66	UTSW	7	109457542	splice site	probably benign
R0568:Trim66	UTSW	7	109460695	missense	probably benign	0.00
R0669:Trim66	UTSW	7	109454992	intron	probably benign
R0980:Trim66	UTSW	7	109455670	missense	probably damaging	1.00
R1015:Trim66	UTSW	7	109455233	missense	probably damaging	1.00
R1078:Trim66	UTSW	7	109472319	missense	probably damaging	1.00
R1099:Trim66	UTSW	7	109475454	missense	probably benign	0.34
R1497:Trim66	UTSW	7	109484619	missense	probably benign	0.00
R1583:Trim66	UTSW	7	109455080	missense	probably damaging	1.00
R1843:Trim66	UTSW	7	109475839	missense	probably damaging	0.99
R1998:Trim66	UTSW	7	109484577	critical splice donor site	probably null
R2016:Trim66	UTSW	7	109472232	critical splice donor site	probably null
R2143:Trim66	UTSW	7	109475113	missense	probably damaging	0.98
R2144:Trim66	UTSW	7	109475113	missense	probably damaging	0.98
R2145:Trim66	UTSW	7	109475113	missense	probably damaging	0.98
R3945:Trim66	UTSW	7	109472268	missense	possibly damaging	0.94
R4012:Trim66	UTSW	7	109458131	missense	probably damaging	0.98
R4464:Trim66	UTSW	7	109477690	missense	possibly damaging	0.51
R4473:Trim66	UTSW	7	109481995	missense	probably damaging	1.00
R4729:Trim66	UTSW	7	109456060	critical splice donor site	probably null
R4730:Trim66	UTSW	7	109483069	missense	probably damaging	1.00
R4775:Trim66	UTSW	7	109457589	nonsense	probably null
R4819:Trim66	UTSW	7	109457586	missense	probably damaging	1.00
R5269:Trim66	UTSW	7	109457590	missense	probably benign	0.00
R5557:Trim66	UTSW	7	109483737	missense	probably benign	0.06
R5832:Trim66	UTSW	7	109455202	missense	probably damaging	1.00
R6220:Trim66	UTSW	7	109483093	missense	probably damaging	0.97
R6243:Trim66	UTSW	7	109460274	missense	probably benign	0.01
R6374:Trim66	UTSW	7	109486062	missense	probably benign
R6450:Trim66	UTSW	7	109460738	missense	probably benign	0.09
R6543:Trim66	UTSW	7	109475879	missense	probably benign	0.01
R6788:Trim66	UTSW	7	109477754	missense	probably damaging	1.00
R6842:Trim66	UTSW	7	109460776	missense	probably benign	0.00
R7169:Trim66	UTSW	7	109455121	missense	probably benign	0.25
R7257:Trim66	UTSW	7	109460244	missense	probably damaging	1.00
R7328:Trim66	UTSW	7	109457751	missense	probably damaging	0.99
R7616:Trim66	UTSW	7	109483749	missense	probably damaging	0.99
RF013:Trim66	UTSW	7	109460753	missense	probably damaging	0.99
RF024:Trim66	UTSW	7	109460740	missense	possibly damaging	0.62

Predicted Primers

PCR Primer
(F):5'- GGCCTAGCTTCTGTGATTCAAGACC -3'
(R):5'- TGCTGCATACAGATGCAAGCCC -3'

Sequencing Primer
(F):5'- TCTGTGATTCAAGACCACACTC -3'
(R):5'- GTCACAGGTCACCAATTTTGGAC -3'

Posted On

2014-01-15