Mutagenetix > Incidental Mutation

Incidental Mutation 'R1187:Vmn2r8'

102246

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r8

Ensembl Gene

ENSMUSG00000090961

Gene Name

vomeronasal 2, receptor 8

Synonyms

EG627479

MMRRC Submission

039259-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.069) question?

Stock #

R1187 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

108797193-108808754 bp(-) (GRCm38)

Type of Mutation

nonsense

DNA Base Change (assembly)

G to T at 108803219 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Serine to Stop codon at position 120 (S120*)

Ref Sequence

ENSEMBL: ENSMUSP00000126953 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000172140]

AlphaFold

L7N472

Predicted Effect

probably null
Transcript: ENSMUST00000172140
AA Change: S120*

SMART Domains

Protein: ENSMUSP00000126953
Gene: ENSMUSG00000090961
AA Change: S120*

Domain	Start	End	E-Value	Type
signal peptide	1	21	N/A	INTRINSIC
Pfam:ANF_receptor	78	419	1.1e-28	PFAM
Pfam:NCD3G	507	561	8.2e-18	PFAM
Pfam:7tm_3	594	829	1.1e-54	PFAM

Coding Region Coverage

1x: 98.9%
3x: 97.9%
10x: 95.1%
20x: 89.2%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 50 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca13	G	T	11: 9,528,981	M4276I	probably benign	Het
Apol7b	A	T	15: 77,423,403	F297L	possibly damaging	Het
C330027C09Rik	T	A	16: 49,000,293	N132K	probably damaging	Het
Capsl	C	T	15: 9,457,721	R9W	probably damaging	Het
Catsperb	T	C	12: 101,625,732	V1107A	probably benign	Het
Clca1	T	C	3: 145,009,743	I544M	probably benign	Het
Cma2	T	C	14: 55,972,823	V55A	probably benign	Het
Col10a1	A	T	10: 34,394,838	I269F	probably benign	Het
Col26a1	G	A	5: 136,744,166	H385Y	probably damaging	Het
Ctdp1	A	G	18: 80,449,487	Y598H	probably damaging	Het
Dnajc1	A	G	2: 18,284,709	S296P	probably benign	Het
Dvl2	A	T	11: 70,006,136	T250S	probably benign	Het
Etv1	A	G	12: 38,865,564	Y410C	probably damaging	Het
Gm38394	G	A	1: 133,659,203	T132I	probably damaging	Het
Krcc1	A	T	6: 71,284,628	K215*	probably null	Het
Lrr1	A	T	12: 69,175,022	T313S	probably benign	Het
Lrrc7	T	A	3: 158,160,402	E1234V	probably damaging	Het
Mepe	G	A	5: 104,338,248	R418H	probably damaging	Het
Myrfl	G	A	10: 116,831,542	T331I	probably damaging	Het
Nbeal1	T	C	1: 60,194,528	W26R	probably damaging	Het
Nsrp1	G	A	11: 77,046,027	P448S	probably benign	Het
Ofcc1	C	T	13: 40,208,829	G206R	probably benign	Het
Olfml2a	A	G	2: 38,959,813	N514D	probably damaging	Het
Olfr1390	T	G	11: 49,340,590	D19E	probably damaging	Het
Olfr167	T	C	16: 19,515,046	T197A	probably benign	Het
Pcdhb1	C	T	18: 37,265,544	R183C	probably damaging	Het
Phf14	T	G	6: 11,941,496	C41G	probably damaging	Het
Pkhd1l1	T	C	15: 44,498,051	V499A	possibly damaging	Het
Plcb4	A	G	2: 135,968,394	I638V	probably benign	Het
Ppp4c	T	A	7: 126,786,200	I296F	probably benign	Het
Prkdc	T	C	16: 15,759,746	V2388A	probably damaging	Het
Rgl1	T	C	1: 152,544,433	D353G	probably benign	Het
Rnf19b	T	G	4: 129,075,567		probably null	Het
Ryr3	C	T	2: 112,958,176	D190N	probably damaging	Het
Sdk2	C	T	11: 113,838,646		silent	Het
Sema3e	G	A	5: 14,232,084	M411I	probably damaging	Het
Sgsh	A	T	11: 119,346,578	Y403*	probably null	Het
Sh2d7	T	A	9: 54,541,187	L164Q	probably benign	Het
Slfn8	A	T	11: 83,003,488	V775E	probably damaging	Het
Smgc	G	A	15: 91,860,600	G287S	probably damaging	Het
Soat1	T	A	1: 156,434,175	Y421F	probably damaging	Het
Sri	T	C	5: 8,059,416	Y52H	probably damaging	Het
Stat4	C	A	1: 52,076,677	Q259K	probably damaging	Het
Trpm5	A	G	7: 143,074,469	L1023P	probably damaging	Het
Ush1c	T	A	7: 46,208,914	N650I	probably benign	Het
Zbtb14	C	A	17: 69,388,502	F398L	probably damaging	Het
Zfp629	A	G	7: 127,610,229	S803P	probably benign	Het
Zfp629	T	C	7: 127,611,887	K250R	probably damaging	Het
Zfp758	T	A	17: 22,375,190	I187N	probably benign	Het
Zfp85	T	A	13: 67,749,716	K79I	probably damaging	Het

Other mutations in Vmn2r8

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02957:Vmn2r8	APN	5	108802225	missense	probably benign	0.01
R0324:Vmn2r8	UTSW	5	108797941	splice site	probably null
R0335:Vmn2r8	UTSW	5	108797451	splice site	probably null
R0394:Vmn2r8	UTSW	5	108802072	missense	probably benign	0.12
R0501:Vmn2r8	UTSW	5	108803183	missense	probably benign	0.03
R0615:Vmn2r8	UTSW	5	108799329	missense	probably damaging	1.00
R0678:Vmn2r8	UTSW	5	108800546	missense	probably benign	0.00
R1167:Vmn2r8	UTSW	5	108803176	missense	probably benign	0.01
R1406:Vmn2r8	UTSW	5	108802368	missense	probably benign
R1406:Vmn2r8	UTSW	5	108802368	missense	probably benign
R1451:Vmn2r8	UTSW	5	108798067	missense	probably damaging	1.00
R1535:Vmn2r8	UTSW	5	108802174	missense	probably damaging	1.00
R1795:Vmn2r8	UTSW	5	108803106	missense	probably benign
R1874:Vmn2r8	UTSW	5	108802418	missense	possibly damaging	0.74
R1908:Vmn2r8	UTSW	5	108797570	missense	probably benign	0.03
R1925:Vmn2r8	UTSW	5	108802153	missense	probably damaging	0.97
R1960:Vmn2r8	UTSW	5	108799286	missense	probably damaging	0.99
R1961:Vmn2r8	UTSW	5	108798095	missense	probably benign	0.45
R1967:Vmn2r8	UTSW	5	108802383	missense	probably benign	0.01
R2095:Vmn2r8	UTSW	5	108808621	missense	possibly damaging	0.94
R2159:Vmn2r8	UTSW	5	108802303	missense	probably benign	0.22
R4240:Vmn2r8	UTSW	5	108797503	missense	probably damaging	0.99
R4581:Vmn2r8	UTSW	5	108801704	missense	probably benign	0.03
R4744:Vmn2r8	UTSW	5	108808581	missense	probably benign	0.00
R4755:Vmn2r8	UTSW	5	108801700	missense	probably benign	0.03
R4917:Vmn2r8	UTSW	5	108797398	missense	probably damaging	1.00
R4957:Vmn2r8	UTSW	5	108799263	missense	probably benign	0.16
R5141:Vmn2r8	UTSW	5	108808706	missense	probably damaging	0.96
R5481:Vmn2r8	UTSW	5	108801770	missense	probably benign	0.09
R5571:Vmn2r8	UTSW	5	108802240	missense	probably damaging	1.00
R5624:Vmn2r8	UTSW	5	108802459	missense	probably damaging	0.99
R6003:Vmn2r8	UTSW	5	108797382	missense	probably damaging	1.00
R6243:Vmn2r8	UTSW	5	108799345	missense	probably benign	0.01
R6265:Vmn2r8	UTSW	5	108808597	missense	probably benign
R6315:Vmn2r8	UTSW	5	108801891	missense	probably benign
R6413:Vmn2r8	UTSW	5	108801723	missense	probably benign	0.09
R7120:Vmn2r8	UTSW	5	108808638	missense	possibly damaging	0.56
R7406:Vmn2r8	UTSW	5	108800576	missense	probably benign	0.00
R7409:Vmn2r8	UTSW	5	108808583	nonsense	probably null
R7489:Vmn2r8	UTSW	5	108797656	missense	possibly damaging	0.95
R7532:Vmn2r8	UTSW	5	108802240	missense	probably benign	0.22
R7534:Vmn2r8	UTSW	5	108802174	missense	possibly damaging	0.94
R7739:Vmn2r8	UTSW	5	108802177	missense	probably damaging	1.00
R8099:Vmn2r8	UTSW	5	108801834	missense	probably benign
R8245:Vmn2r8	UTSW	5	108798070	missense	probably damaging	1.00
R8711:Vmn2r8	UTSW	5	108798096	missense	possibly damaging	0.89
R8781:Vmn2r8	UTSW	5	108797731	missense	possibly damaging	0.95
R8874:Vmn2r8	UTSW	5	108808751	missense	probably damaging	1.00
R8927:Vmn2r8	UTSW	5	108802265	missense
R8928:Vmn2r8	UTSW	5	108802265	missense
R9288:Vmn2r8	UTSW	5	108802319	missense	probably benign	0.39
R9596:Vmn2r8	UTSW	5	108799330	missense	possibly damaging	0.94
R9652:Vmn2r8	UTSW	5	108803241	missense	probably benign	0.18
Z1088:Vmn2r8	UTSW	5	108801998	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- GCTGTCTAAGTTTTCTTTCAGGCACG -3'
(R):5'- GGCAGTACTCTGGCTCTCCTTAGTATAA -3'

Sequencing Primer
(F):5'- acagtggctcacaaccatc -3'
(R):5'- TCTAGATCAAGGTCAGCATTCAGAG -3'

Posted On

2014-01-15