Mutagenetix > Incidental Mutation

Incidental Mutation 'IGL01707:Wif1'

104657

Institutional Source

Australian Phenomics Network (link to record)

Gene Symbol

Wif1

Ensembl Gene

ENSMUSG00000020218

Gene Name

Wnt inhibitory factor 1

Synonyms

WIF-1

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

IGL01707

Quality Score

Status

Chromosome

Chromosomal Location

120869909-120936547 bp(+) (GRCm39)

Type of Mutation

critical splice donor site (2 bp from exon)

DNA Base Change (assembly)

T to A at 120919890 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000135486 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000020439] [ENSMUST00000175867]

AlphaFold

Q9WUA1

Predicted Effect

probably null
Transcript: ENSMUST00000020439

SMART Domains

Protein: ENSMUSP00000020439
Gene: ENSMUSG00000020218

Domain	Start	End	E-Value	Type
signal peptide	1	28	N/A	INTRINSIC
WIF	35	179	8.47e-90	SMART
EGF	181	210	3.88e-3	SMART
EGF	213	242	4.46e-3	SMART
EGF	245	274	4.7e-2	SMART
EGF	277	306	1.69e-3	SMART
EGF	309	338	7.95e0	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000145691

Predicted Effect

probably null
Transcript: ENSMUST00000175867

SMART Domains

Protein: ENSMUSP00000135486
Gene: ENSMUSG00000020218

Domain	Start	End	E-Value	Type
signal peptide	1	28	N/A	INTRINSIC
WIF	35	179	8.47e-90	SMART
EGF	181	210	3.88e-3	SMART
EGF	213	242	4.46e-3	SMART
EGF	245	274	4.7e-2	SMART
EGF	295	324	7.95e0	SMART

Coding Region Coverage

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene functions to inhibit WNT proteins, which are extracellular signaling molecules that play a role in embryonic development. This protein contains a WNT inhibitory factor (WIF) domain and five epidermal growth factor (EGF)-like domains, and is thought to be involved in mesoderm segmentation. This gene functions as a tumor suppressor gene, and has been found to be epigenetically silenced in various cancers. [provided by RefSeq, Jun 2010]
PHENOTYPE: Homozygous null mice are viable and fertile but display increased susceptibility to spontaneous and induced osteosarcomas. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 33 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ablim1	C	A	19: 57,027,879 (GRCm39)	R404L	probably damaging	Het
Acot6	C	A	12: 84,147,763 (GRCm39)	S6R	probably benign	Het
Adamtsl4	T	A	3: 95,591,301 (GRCm39)	Q222L	probably benign	Het
Adgre5	T	A	8: 84,450,976 (GRCm39)	T725S	probably damaging	Het
Agbl3	A	T	6: 34,816,389 (GRCm39)	K766N	possibly damaging	Het
Ankrd12	A	G	17: 66,291,273 (GRCm39)	S1387P	probably damaging	Het
Camk2a	G	A	18: 61,093,122 (GRCm39)		probably null	Het
Cd209d	T	C	8: 3,928,296 (GRCm39)	I30V	probably benign	Het
Ces1d	A	T	8: 93,916,178 (GRCm39)	S150T	possibly damaging	Het
Cox4i2	T	C	2: 152,598,956 (GRCm39)	Y38H	probably damaging	Het
Csmd2	T	C	4: 128,276,798 (GRCm39)	Y867H	possibly damaging	Het
Dbh	G	A	2: 27,055,556 (GRCm39)	C10Y	probably benign	Het
Dnajc13	A	G	9: 104,106,178 (GRCm39)	V332A	probably damaging	Het
Gnb3	G	T	6: 124,816,652 (GRCm39)	A11E	possibly damaging	Het
Herc2	A	G	7: 55,814,935 (GRCm39)	R2725G	probably damaging	Het
Kdm4c	C	T	4: 74,255,164 (GRCm39)	L573F	probably damaging	Het
Kmt2c	T	A	5: 25,505,096 (GRCm39)	Q3404L	probably damaging	Het
Mmp9	A	G	2: 164,791,909 (GRCm39)	H231R	probably benign	Het
Nphp3	A	T	9: 103,895,357 (GRCm39)	D371V	possibly damaging	Het
Nphp4	A	G	4: 152,623,440 (GRCm39)	I705V	probably benign	Het
Nutm2	A	T	13: 50,623,753 (GRCm39)	N150I	probably damaging	Het
Or51a7	T	C	7: 102,615,126 (GRCm39)	I273T	probably damaging	Het
Or51aa5	A	T	7: 103,167,141 (GRCm39)	I150N	probably damaging	Het
Phb2	A	G	6: 124,690,998 (GRCm39)	Q52R	probably benign	Het
Plekhh1	T	A	12: 79,125,738 (GRCm39)	V1258E	probably benign	Het
Pou2f1	A	T	1: 165,742,685 (GRCm39)	N69K	probably damaging	Het
Rapsn	T	A	2: 90,873,585 (GRCm39)	M297K	probably benign	Het
Senp5	A	C	16: 31,808,588 (GRCm39)	V195G	probably damaging	Het
Trim25	T	C	11: 88,890,517 (GRCm39)	V68A	probably damaging	Het
Ttc41	A	T	10: 86,612,631 (GRCm39)	R1301S	probably damaging	Het
Vcan	T	C	13: 89,837,864 (GRCm39)	Y1600C	probably damaging	Het
Washc5	A	G	15: 59,213,864 (GRCm39)	I864T	possibly damaging	Het
Zdhhc15	G	A	X: 103,609,422 (GRCm39)	R208C	probably damaging	Het

Other mutations in Wif1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01384:Wif1	APN	10	120,920,855 (GRCm39)	missense	possibly damaging	0.55
IGL01932:Wif1	APN	10	120,931,940 (GRCm39)	missense	probably damaging	1.00
IGL02183:Wif1	APN	10	120,911,181 (GRCm39)	missense	probably damaging	1.00
R0653:Wif1	UTSW	10	120,935,704 (GRCm39)	missense	probably benign	0.01
R1858:Wif1	UTSW	10	120,919,788 (GRCm39)	splice site	probably null
R1869:Wif1	UTSW	10	120,920,824 (GRCm39)	missense	probably benign	0.00
R1871:Wif1	UTSW	10	120,920,824 (GRCm39)	missense	probably benign	0.00
R4056:Wif1	UTSW	10	120,918,099 (GRCm39)	missense	probably benign	0.04
R4057:Wif1	UTSW	10	120,918,099 (GRCm39)	missense	probably benign	0.04
R5056:Wif1	UTSW	10	120,935,684 (GRCm39)	missense	probably benign	0.00
R6242:Wif1	UTSW	10	120,870,366 (GRCm39)	missense	possibly damaging	0.82
R6504:Wif1	UTSW	10	120,870,996 (GRCm39)	missense	probably damaging	0.96
R7220:Wif1	UTSW	10	120,926,019 (GRCm39)	missense	possibly damaging	0.76
R7365:Wif1	UTSW	10	120,919,814 (GRCm39)	missense	possibly damaging	0.94
R7456:Wif1	UTSW	10	120,932,554 (GRCm39)	missense	probably benign
R7707:Wif1	UTSW	10	120,919,864 (GRCm39)	missense	probably damaging	0.97
R8283:Wif1	UTSW	10	120,931,952 (GRCm39)	missense	probably benign
R8817:Wif1	UTSW	10	120,932,621 (GRCm39)	missense	possibly damaging	0.95
R8940:Wif1	UTSW	10	120,935,684 (GRCm39)	missense	probably benign	0.11
R8959:Wif1	UTSW	10	120,931,957 (GRCm39)	missense	probably damaging	0.99
R9628:Wif1	UTSW	10	120,932,549 (GRCm39)	missense	possibly damaging	0.94
Z1176:Wif1	UTSW	10	120,932,561 (GRCm39)	missense	probably damaging	1.00

Posted On

2014-01-21