Incidental Mutation 'R1270:Mrgpra9'
| ID |
151275 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Mrgpra9
|
| Ensembl Gene |
ENSMUSG00000074111 |
| Gene Name |
MAS-related GPR, member A9 |
| Synonyms |
EG668725, MrgA9 |
| MMRRC Submission |
039336-MU
|
| Accession Numbers |
|
| Essential gene? |
Probably non essential
(E-score: 0.054)
|
| Stock # |
R1270 (G1)
|
| Quality Score |
225 |
|
Status
|
Validated
|
| Chromosome |
7 |
| Chromosomal Location |
47234861-47252848 bp(-) (GRCm38) |
| Type of Mutation |
critical splice donor site (2 bp from exon) |
| DNA Base Change (assembly) |
A to T
at 47252783 bp (GRCm38)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000136396
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000098436]
[ENSMUST00000179005]
|
| AlphaFold |
A0A140T8U8 |
| Predicted Effect |
probably null
Transcript: ENSMUST00000098436
|
| SMART Domains |
Protein: ENSMUSP00000096035
Gene: ENSMUSG00000074111
| Domain | Start | End | E-Value | Type |
|---|
|
low complexity region
|
37 |
48 |
N/A |
INTRINSIC |
|
Pfam:7tm_1
|
56 |
225 |
1.5e-11 |
PFAM |
|
| Predicted Effect |
probably null
Transcript: ENSMUST00000179005
|
| SMART Domains |
Protein: ENSMUSP00000136396
Gene: ENSMUSG00000074111
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:7tm_1
|
12 |
178 |
3.4e-9 |
PFAM |
|
| Meta Mutation Damage Score |
0.9755 |
| Coding Region Coverage |
- 1x: 98.9%
- 3x: 98.1%
- 10x: 95.5%
- 20x: 89.7%
|
| Validation Efficiency |
97% (36/37) |
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 32 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| 4932438A13Rik |
T |
A |
3: 36,952,184 (GRCm38) |
H1662Q |
probably damaging |
Het |
| Abcc3 |
C |
T |
11: 94,357,384 (GRCm38) |
R1129Q |
probably damaging |
Het |
| Adam7 |
T |
A |
14: 68,527,669 (GRCm38) |
K93M |
probably damaging |
Het |
| Aldh1a2 |
T |
G |
9: 71,281,706 (GRCm38) |
L301V |
probably benign |
Het |
| Alg9 |
A |
G |
9: 50,787,572 (GRCm38) |
|
probably benign |
Het |
| Aspg |
C |
T |
12: 112,116,447 (GRCm38) |
T187I |
probably damaging |
Het |
| C4a |
A |
G |
17: 34,814,528 (GRCm38) |
|
noncoding transcript |
Het |
| Cdh2 |
T |
G |
18: 16,627,557 (GRCm38) |
|
probably benign |
Het |
| Ceacam1 |
T |
C |
7: 25,466,314 (GRCm38) |
|
probably null |
Het |
| Cep250 |
G |
A |
2: 155,990,681 (GRCm38) |
V1509I |
probably benign |
Het |
| D130043K22Rik |
T |
C |
13: 24,857,338 (GRCm38) |
S248P |
probably benign |
Het |
| Dgkd |
A |
T |
1: 87,934,125 (GRCm38) |
M801L |
probably damaging |
Het |
| E330021D16Rik |
A |
T |
6: 136,401,787 (GRCm38) |
I15N |
probably damaging |
Het |
| Edc4 |
A |
G |
8: 105,891,264 (GRCm38) |
E1152G |
possibly damaging |
Het |
| Enkd1 |
A |
G |
8: 105,703,901 (GRCm38) |
I334T |
probably damaging |
Het |
| Gli3 |
C |
T |
13: 15,723,744 (GRCm38) |
A803V |
probably benign |
Het |
| Glrx3 |
A |
G |
7: 137,453,414 (GRCm38) |
N95S |
probably benign |
Het |
| Ints2 |
C |
T |
11: 86,233,085 (GRCm38) |
G626R |
probably damaging |
Het |
| Kank2 |
A |
T |
9: 21,772,760 (GRCm38) |
N724K |
probably damaging |
Het |
| Kctd20 |
A |
G |
17: 28,966,931 (GRCm38) |
D416G |
possibly damaging |
Het |
| Lmtk3 |
A |
G |
7: 45,793,828 (GRCm38) |
E645G |
probably damaging |
Het |
| Muc1 |
T |
A |
3: 89,232,107 (GRCm38) |
Y605N |
probably damaging |
Het |
| Olfr1256 |
C |
T |
2: 89,835,322 (GRCm38) |
V208M |
possibly damaging |
Het |
| Olfr146 |
A |
C |
9: 39,019,247 (GRCm38) |
I98R |
possibly damaging |
Het |
| Prx |
T |
A |
7: 27,518,930 (GRCm38) |
I952N |
probably damaging |
Het |
| Shf |
G |
A |
2: 122,368,682 (GRCm38) |
P51S |
probably damaging |
Het |
| Skint5 |
A |
T |
4: 113,942,659 (GRCm38) |
Y90* |
probably null |
Het |
| Swt1 |
A |
T |
1: 151,384,391 (GRCm38) |
N752K |
probably benign |
Het |
| Taar1 |
T |
C |
10: 23,920,533 (GRCm38) |
V43A |
probably damaging |
Het |
| Tenm2 |
T |
C |
11: 36,041,659 (GRCm38) |
N1702D |
probably damaging |
Het |
| Tff2 |
C |
T |
17: 31,144,169 (GRCm38) |
|
probably null |
Het |
| Trim2 |
G |
A |
3: 84,167,677 (GRCm38) |
A686V |
probably damaging |
Het |
|
| Other mutations in Mrgpra9 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL00095:Mrgpra9
|
APN |
7 |
47,235,091 (GRCm38) |
missense |
possibly damaging |
0.85 |
|
IGL00575:Mrgpra9
|
APN |
7 |
47,235,305 (GRCm38) |
missense |
possibly damaging |
0.76 |
|
IGL01649:Mrgpra9
|
APN |
7 |
47,235,152 (GRCm38) |
missense |
probably benign |
0.22 |
|
IGL03207:Mrgpra9
|
APN |
7 |
47,235,637 (GRCm38) |
missense |
possibly damaging |
0.68 |
|
R0388:Mrgpra9
|
UTSW |
7 |
47,252,794 (GRCm38) |
start codon destroyed |
probably null |
0.08 |
|
R0972:Mrgpra9
|
UTSW |
7 |
47,235,455 (GRCm38) |
missense |
probably damaging |
0.99 |
|
R1381:Mrgpra9
|
UTSW |
7 |
47,235,302 (GRCm38) |
missense |
possibly damaging |
0.75 |
|
R1403:Mrgpra9
|
UTSW |
7 |
47,235,638 (GRCm38) |
missense |
probably benign |
0.24 |
|
R1403:Mrgpra9
|
UTSW |
7 |
47,235,638 (GRCm38) |
missense |
probably benign |
0.24 |
|
R1448:Mrgpra9
|
UTSW |
7 |
47,235,813 (GRCm38) |
missense |
probably benign |
0.02 |
|
R2045:Mrgpra9
|
UTSW |
7 |
47,235,835 (GRCm38) |
missense |
probably benign |
|
|
R2144:Mrgpra9
|
UTSW |
7 |
47,235,463 (GRCm38) |
missense |
probably benign |
0.31 |
|
R2187:Mrgpra9
|
UTSW |
7 |
47,235,049 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R2507:Mrgpra9
|
UTSW |
7 |
47,235,494 (GRCm38) |
missense |
possibly damaging |
0.63 |
|
R2913:Mrgpra9
|
UTSW |
7 |
47,235,080 (GRCm38) |
missense |
probably benign |
|
|
R3810:Mrgpra9
|
UTSW |
7 |
47,235,779 (GRCm38) |
missense |
probably damaging |
0.98 |
|
R4177:Mrgpra9
|
UTSW |
7 |
47,235,554 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R4521:Mrgpra9
|
UTSW |
7 |
47,235,190 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R4781:Mrgpra9
|
UTSW |
7 |
47,235,047 (GRCm38) |
missense |
possibly damaging |
0.88 |
|
R4926:Mrgpra9
|
UTSW |
7 |
47,235,011 (GRCm38) |
missense |
possibly damaging |
0.62 |
|
R6469:Mrgpra9
|
UTSW |
7 |
47,235,106 (GRCm38) |
missense |
probably benign |
0.02 |
|
R6505:Mrgpra9
|
UTSW |
7 |
47,235,136 (GRCm38) |
missense |
probably benign |
0.00 |
|
R6724:Mrgpra9
|
UTSW |
7 |
47,235,038 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R7398:Mrgpra9
|
UTSW |
7 |
47,235,637 (GRCm38) |
missense |
possibly damaging |
0.68 |
|
R8737:Mrgpra9
|
UTSW |
7 |
47,235,876 (GRCm38) |
missense |
probably benign |
0.00 |
|
R8824:Mrgpra9
|
UTSW |
7 |
47,235,293 (GRCm38) |
missense |
probably benign |
0.06 |
|
R8881:Mrgpra9
|
UTSW |
7 |
47,235,494 (GRCm38) |
missense |
possibly damaging |
0.63 |
|
R9320:Mrgpra9
|
UTSW |
7 |
47,235,644 (GRCm38) |
missense |
probably benign |
0.00 |
|
| Predicted Primers |
PCR Primer
(F):5'- CAGCTTCTAACCACTCACTGTCCAG -3'
(R):5'- AGGTGGCAGGAAACTCCAATCAAC -3'
Sequencing Primer
(F):5'- GCCTGTCTGCACCAAACTC -3'
(R):5'- CCAGAGATTGAGAACTGTCTCAGG -3'
|
| Posted On |
2014-01-29 |
Incidental Mutation