Mutagenetix > Incidental Mutation

Incidental Mutation 'R1256:Tstd3'

151445

Institutional Source

Beutler Lab

Gene Symbol

Tstd3

Ensembl Gene

ENSMUSG00000028251

Gene Name

thiosulfate sulfurtransferase (rhodanese)-like domain containing 3

Synonyms

2610029I01Rik

MMRRC Submission

039323-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.072) question?

Stock #

R1256 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

21757382-21767211 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 21759627 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Methionine at position 79 (I79M)

Ref Sequence

ENSEMBL: ENSMUSP00000029915 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000029915] [ENSMUST00000120679]

AlphaFold

Q9D0B5

Predicted Effect

probably damaging
Transcript: ENSMUST00000029915
AA Change: I79M

PolyPhen 2 Score 0.970 (Sensitivity: 0.77; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000029915
Gene: ENSMUSG00000028251
AA Change: I79M

Domain	Start	End	E-Value	Type
RHOD	42	151	1.6e-16	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000120679

SMART Domains

Protein: ENSMUSP00000113682
Gene: ENSMUSG00000028252

Domain	Start	End	E-Value	Type
CYCLIN	46	144	2.41e-13	SMART
CYCLIN	157	236	4.11e-7	SMART
low complexity region	258	264	N/A	INTRINSIC

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 96.9%
20x: 94.4%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 38 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcb10	C	T	8: 124,688,791 (GRCm39)	G495D	probably damaging	Het
Apaf1	A	G	10: 90,894,268 (GRCm39)	Y456H	probably benign	Het
Arhgef11	A	G	3: 87,634,442 (GRCm39)	N791S	possibly damaging	Het
Brat1	A	G	5: 140,695,962 (GRCm39)	Q66R	possibly damaging	Het
Capn10	C	T	1: 92,874,668 (GRCm39)	T633M	probably damaging	Het
Ccdc186	G	T	19: 56,786,053 (GRCm39)	L661I	probably benign	Het
Cln3	A	G	7: 126,182,208 (GRCm39)	S4P	probably damaging	Het
Cnot10	G	T	9: 114,439,749 (GRCm39)	S520Y	probably damaging	Het
Csmd1	A	T	8: 16,129,978 (GRCm39)	F1715I	probably damaging	Het
Ezh2	A	T	6: 47,518,789 (GRCm39)	C545*	probably null	Het
Hivep1	C	T	13: 42,335,307 (GRCm39)	S2282F	probably damaging	Het
Jag2	T	C	12: 112,878,039 (GRCm39)	E564G	possibly damaging	Het
Kcnj16	A	G	11: 110,916,262 (GRCm39)	H308R	probably damaging	Het
Magi3	C	T	3: 103,935,126 (GRCm39)	V936I	probably benign	Het
Mcu	G	T	10: 59,290,790 (GRCm39)	A279E	probably damaging	Het
Msln	A	G	17: 25,973,157 (GRCm39)	C13R	probably damaging	Het
Nes	A	G	3: 87,883,883 (GRCm39)	D714G	probably benign	Het
Nif3l1	T	A	1: 58,494,808 (GRCm39)	V259D	probably damaging	Het
Or4c127	A	T	2: 89,832,911 (GRCm39)	M54L	possibly damaging	Het
Or5k14	A	C	16: 58,693,206 (GRCm39)	Y102*	probably null	Het
Or8g18	G	C	9: 39,149,547 (GRCm39)	P58A	probably benign	Het
Pcdhb9	A	G	18: 37,536,169 (GRCm39)	D721G	possibly damaging	Het
Pepd	A	C	7: 34,620,917 (GRCm39)	T61P	possibly damaging	Het
Pkd1l2	C	T	8: 117,746,282 (GRCm39)		probably null	Het
Poglut3	A	C	9: 53,299,762 (GRCm39)	R90S	possibly damaging	Het
Psip1	A	G	4: 83,392,604 (GRCm39)	S102P	probably benign	Het
Ralgapa1	T	C	12: 55,809,446 (GRCm39)	E443G	possibly damaging	Het
Rnf20	A	G	4: 49,638,230 (GRCm39)	D114G	probably benign	Het
Schip1	A	T	3: 68,402,375 (GRCm39)	I151F	probably benign	Het
Smarca2	A	G	19: 26,659,373 (GRCm39)	I888V	probably benign	Het
Smg1	T	C	7: 117,802,310 (GRCm39)	T263A	probably damaging	Het
Spn	T	C	7: 126,735,445 (GRCm39)	K354R	possibly damaging	Het
Sspo	G	A	6: 48,434,573 (GRCm39)	A1022T	probably damaging	Het
Strn	A	C	17: 78,972,046 (GRCm39)		probably null	Het
Txnl4a	A	G	18: 80,250,487 (GRCm39)	I28V	probably benign	Het
Uty	T	C	Y: 1,134,884 (GRCm39)	D928G	probably damaging	Het
Vps26c	A	G	16: 94,313,225 (GRCm39)	L22P	probably damaging	Het
Vsx2	T	A	12: 84,623,085 (GRCm39)	L163Q	probably damaging	Het

Other mutations in Tstd3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02108:Tstd3	APN	4	21,759,366 (GRCm39)	intron	probably benign
R1699:Tstd3	UTSW	4	21,759,400 (GRCm39)	missense	probably benign	0.01
R1771:Tstd3	UTSW	4	21,759,475 (GRCm39)	missense	probably damaging	1.00
R1776:Tstd3	UTSW	4	21,759,475 (GRCm39)	missense	probably damaging	1.00
R2847:Tstd3	UTSW	4	21,759,375 (GRCm39)	missense	possibly damaging	0.90
R5047:Tstd3	UTSW	4	21,759,621 (GRCm39)	critical splice donor site	probably null
R5838:Tstd3	UTSW	4	21,759,622 (GRCm39)	critical splice donor site	probably null
R6960:Tstd3	UTSW	4	21,767,118 (GRCm39)	start codon destroyed	probably null	0.01
R9005:Tstd3	UTSW	4	21,767,082 (GRCm39)	missense	possibly damaging	0.49
X0058:Tstd3	UTSW	4	21,759,779 (GRCm39)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- TCACACACCTGTTAAAGCCCAGTG -3'
(R):5'- AGTCAATATGGAGATGCAGCCAAGC -3'

Sequencing Primer
(F):5'- TGATATTGCTGTGTCCATAGCC -3'
(R):5'- GATGCAGCCAAGCATTTTGTTC -3'

Posted On

2014-01-29