Mutagenetix > Incidental Mutation

Incidental Mutation 'R1263:Zfp277'

151673

Institutional Source

Beutler Lab

Gene Symbol

Zfp277

Ensembl Gene

ENSMUSG00000055917

Gene Name

zinc finger protein 277

Synonyms

2410017E24Rik, NIRF4

MMRRC Submission

039330-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.072) question?

Stock #

R1263 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

40315046-40445902 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 40364165 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Asparagine at position 227 (I227N)

Ref Sequence

ENSEMBL: ENSMUSP00000064226 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000069637] [ENSMUST00000069692]

AlphaFold

E9Q6D6

Predicted Effect

possibly damaging
Transcript: ENSMUST00000069637
AA Change: I101N

PolyPhen 2 Score 0.793 (Sensitivity: 0.85; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000068032
Gene: ENSMUSG00000055917
AA Change: I101N

Domain	Start	End	E-Value	Type
ZnF_C2H2	59	84	4.27e1	SMART
coiled coil region	143	171	N/A	INTRINSIC
ZnF_C2H2	174	198	3.85e1	SMART
ZnF_C2H2	225	249	2.24e-3	SMART
low complexity region	280	292	N/A	INTRINSIC
ZnF_C2H2	303	326	1.91e1	SMART
ZnF_C2H2	356	382	4.94e0	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000069692
AA Change: I227N

PolyPhen 2 Score 0.987 (Sensitivity: 0.73; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000064226
Gene: ENSMUSG00000055917
AA Change: I227N

Domain	Start	End	E-Value	Type
ZnF_C2H2	185	210	4.27e1	SMART
coiled coil region	269	297	N/A	INTRINSIC
ZnF_C2H2	300	324	3.85e1	SMART
ZnF_C2H2	351	375	2.24e-3	SMART
low complexity region	406	418	N/A	INTRINSIC
ZnF_C2H2	429	452	1.91e1	SMART
ZnF_C2H2	482	508	4.94e0	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000221847

Coding Region Coverage

1x: 99.1%
3x: 98.3%
10x: 96.4%
20x: 93.1%

Validation Efficiency

MGI Phenotype

PHENOTYPE: Mice homozygous for a knock-out allele exhibit early cellular preplicative senescence in MEFs. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 53 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
0610010F05Rik	A	T	11: 23,620,278	Y207*	probably null	Het
Abca8b	A	C	11: 109,941,607	H1231Q	possibly damaging	Het
Acbd4	T	A	11: 103,103,851		probably null	Het
Atp13a4	T	A	16: 29,471,953	Y226F	possibly damaging	Het
Brd3	A	C	2: 27,462,522	F132C	probably damaging	Het
Btaf1	A	T	19: 36,956,524	N184I	probably benign	Het
Ccdc180	A	G	4: 45,903,887	E351G	possibly damaging	Het
Ccdc185	A	T	1: 182,747,353	Y590*	probably null	Het
Chil1	G	A	1: 134,189,242	E315K	probably benign	Het
Col6a6	T	A	9: 105,709,489	M1778L	probably benign	Het
Cyp3a59	A	C	5: 146,104,711	Y355S	probably damaging	Het
Cyp4a31	A	G	4: 115,574,711	T396A	probably benign	Het
Dnah6	A	T	6: 73,144,965	I1373N	probably damaging	Het
Dopey2	C	A	16: 93,777,386	H1598N	probably benign	Het
Erich4	T	A	7: 25,615,134	K118M	probably damaging	Het
Gkap1	A	T	13: 58,255,773	V179E	probably benign	Het
Gpr107	T	G	2: 31,178,255	I243S	possibly damaging	Het
Hs3st6	A	G	17: 24,758,530	N328S	probably damaging	Het
Kcnq5	A	T	1: 21,479,378	I375N	probably damaging	Het
Klhdc3	A	T	17: 46,676,966	H266Q	probably benign	Het
Krt71	C	T	15: 101,735,466	G446R	probably damaging	Het
L3mbtl2	A	G	15: 81,682,968	T423A	probably benign	Het
Mical3	T	C	6: 120,952,469	E1812G	probably damaging	Het
Nlrp1a	A	G	11: 71,097,122	I1174T	probably benign	Het
Npas2	C	A	1: 39,334,768	Q450K	possibly damaging	Het
Nrp1	T	A	8: 128,468,389	I442N	probably damaging	Het
Olfr1385	A	T	11: 49,495,021	M163L	probably benign	Het
Olfr338	T	A	2: 36,376,994	S73T	probably damaging	Het
Palld	TGCGTAGCG	TGCG	8: 61,513,457		probably null	Het
Pih1d3	A	T	1: 31,223,215	I93F	probably damaging	Het
Pold3	T	A	7: 100,119,683	Q36L	possibly damaging	Het
Polg	T	C	7: 79,459,786	T428A	probably benign	Het
Rfx7	T	A	9: 72,577,047	V57E	possibly damaging	Het
Rnf122	T	G	8: 31,112,149	M1R	probably null	Het
Scn10a	T	A	9: 119,617,733	T1410S	probably damaging	Het
Serpinb13	T	A	1: 107,000,736	V362E	probably damaging	Het
Setdb1	T	C	3: 95,327,611	N927S	probably damaging	Het
Sft2d1	A	G	17: 8,320,638	K91R	probably benign	Het
Shprh	A	G	10: 11,159,530	H327R	probably damaging	Het
Slc9b2	C	A	3: 135,336,395	H478Q	probably benign	Het
Styxl1	G	T	5: 135,753,883	S117R	probably damaging	Het
Synj2	T	C	17: 6,019,359	F150L	probably damaging	Het
Tep1	C	A	14: 50,845,513	V1013L	possibly damaging	Het
Tgfbi	T	A	13: 56,630,655	L413Q	probably damaging	Het
Tmc5	G	A	7: 118,666,870	R789Q	probably damaging	Het
Tonsl	T	A	15: 76,622,562	I115F	possibly damaging	Het
Trim38	A	G	13: 23,791,134	Y352C	probably damaging	Het
Txnl4a	T	A	18: 80,207,321	V44D	probably benign	Het
Vars2	A	G	17: 35,661,609	V39A	probably damaging	Het
Vmn2r105	A	G	17: 20,208,322	C831R	probably damaging	Het
Vmn2r26	T	A	6: 124,050,708	I469N	probably benign	Het
Vmn2r53	T	C	7: 12,581,606	Y762C	probably benign	Het
Vps13d	T	A	4: 145,170,348	Q334L	probably benign	Het

Other mutations in Zfp277

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01466:Zfp277	APN	12	40378826	missense	probably benign	0.24
IGL01477:Zfp277	APN	12	40320676	missense	probably benign	0.00
IGL02081:Zfp277	APN	12	40328796	nonsense	probably null
IGL02165:Zfp277	APN	12	40315803	missense	possibly damaging	0.75
IGL02613:Zfp277	APN	12	40329515	missense	probably damaging	1.00
IGL02688:Zfp277	APN	12	40328688	missense	possibly damaging	0.95
IGL02825:Zfp277	APN	12	40317176	missense	probably benign	0.06
R0194:Zfp277	UTSW	12	40378877	splice site	probably benign
R0226:Zfp277	UTSW	12	40364162	missense	possibly damaging	0.67
R0843:Zfp277	UTSW	12	40320600	critical splice donor site	probably null
R1584:Zfp277	UTSW	12	40378826	missense	probably benign	0.12
R1609:Zfp277	UTSW	12	40328720	missense	probably damaging	0.99
R1644:Zfp277	UTSW	12	40329610	splice site	probably null
R1789:Zfp277	UTSW	12	40364085	missense	probably benign	0.00
R1882:Zfp277	UTSW	12	40445746	missense	probably benign	0.03
R2011:Zfp277	UTSW	12	40317218	nonsense	probably null
R4884:Zfp277	UTSW	12	40363153	missense	probably damaging	0.97
R4976:Zfp277	UTSW	12	40328688	missense	possibly damaging	0.95
R5119:Zfp277	UTSW	12	40328688	missense	possibly damaging	0.95
R5532:Zfp277	UTSW	12	40335309	missense	probably damaging	1.00
R6340:Zfp277	UTSW	12	40318549	missense	possibly damaging	0.57
R7191:Zfp277	UTSW	12	40329562	missense	probably damaging	1.00
R7378:Zfp277	UTSW	12	40315853	missense	possibly damaging	0.94
R7446:Zfp277	UTSW	12	40328730	missense	probably damaging	1.00
R7564:Zfp277	UTSW	12	40329595	missense	probably damaging	0.99
R7861:Zfp277	UTSW	12	40315881	missense	possibly damaging	0.92
R8428:Zfp277	UTSW	12	40329578	missense	probably damaging	1.00
R9673:Zfp277	UTSW	12	40320612	missense	probably benign	0.15

Predicted Primers

PCR Primer
(F):5'- TCAGCAATCATGCAATGAGAGCAAGT -3'
(R):5'- CCCCTAATTCCTGCGCTTTAAATGCTTA -3'

Sequencing Primer
(F):5'- CAATGAGAGCAAGTGCAATTTAAGAG -3'
(R):5'- gaaggtagagggtgaaaaatcag -3'

Posted On

2014-01-29