Mutagenetix > Incidental Mutation

Incidental Mutation 'R1251:Tmem95'

151767

Institutional Source

Beutler Lab

Gene Symbol

Tmem95

Ensembl Gene

ENSMUSG00000094845

Gene Name

transmembrane protein 95

Synonyms

432576

MMRRC Submission

039318-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.313) question?

Stock #

R1251 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

69876684-69878018 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 69876829 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Phenylalanine to Serine at position 153 (F153S)

Ref Sequence

ENSEMBL: ENSMUSP00000137492 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000001631] [ENSMUST00000050555] [ENSMUST00000108622] [ENSMUST00000178597]

AlphaFold

P0DJF3

Predicted Effect

probably benign
Transcript: ENSMUST00000001631

SMART Domains

Protein: ENSMUSP00000001631
Gene: ENSMUSG00000001588

Domain	Start	End	E-Value	Type
Pfam:BAR_3	5	240	2.1e-68	PFAM
PH	266	362	4.42e-15	SMART
ArfGap	405	527	2.42e-50	SMART
ANK	606	635	4.01e0	SMART
ANK	639	668	3.04e0	SMART
ANK	672	702	4.18e2	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000050555

SMART Domains

Protein: ENSMUSP00000059107
Gene: ENSMUSG00000046731

Domain	Start	End	E-Value	Type
Pfam:BTB_2	1	73	1.8e-11	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000108622

SMART Domains

Protein: ENSMUSP00000104262
Gene: ENSMUSG00000001588

Domain	Start	End	E-Value	Type
PH	78	174	4.42e-15	SMART
ArfGap	217	339	2.42e-50	SMART
ANK	418	447	4.01e0	SMART
ANK	451	480	3.04e0	SMART
ANK	484	514	4.18e2	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000178597
AA Change: F153S

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000137492
Gene: ENSMUSG00000094845
AA Change: F153S

Domain	Start	End	E-Value	Type
signal peptide	1	16	N/A	INTRINSIC
Pfam:TMEM95	17	168	3.9e-83	PFAM

Coding Region Coverage

1x: 99.1%
3x: 98.3%
10x: 96.3%
20x: 92.6%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 43 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcb10	C	T	8: 123,962,052 (GRCm38)	G495D	probably damaging	Het
Acap2	A	T	16: 31,108,171 (GRCm38)	Y509N	probably damaging	Het
Adcy9	T	A	16: 4,311,531 (GRCm38)	E497V	probably damaging	Het
Bcat2	T	G	7: 45,575,986 (GRCm38)	L56R	probably damaging	Het
Ccdc146	T	C	5: 21,293,372 (GRCm38)	M952V	probably benign	Het
Ccdc39	T	C	3: 33,826,480 (GRCm38)	K446R	probably damaging	Het
Cfap46	C	T	7: 139,601,265 (GRCm38)	V2607I	probably benign	Het
Clec18a	T	C	8: 111,081,638 (GRCm38)	I54V	possibly damaging	Het
Coil	A	G	11: 88,982,299 (GRCm38)	E455G	possibly damaging	Het
Copg1	A	T	6: 87,890,007 (GRCm38)	K75*	probably null	Het
Cyp2j12	G	A	4: 96,115,666 (GRCm38)	Q238*	probably null	Het
D17Wsu92e	C	T	17: 27,786,070 (GRCm38)		probably null	Het
Eif3i	T	C	4: 129,593,385 (GRCm38)	E229G	probably damaging	Het
Exoc2	T	A	13: 30,886,276 (GRCm38)	N411Y	probably benign	Het
Eya2	T	A	2: 165,754,484 (GRCm38)	M305K	probably damaging	Het
Faim	C	T	9: 98,992,634 (GRCm38)	T78M	probably damaging	Het
Fgg	T	A	3: 83,012,980 (GRCm38)	D355E	probably benign	Het
Foxn1	A	G	11: 78,358,785 (GRCm38)	L638P	probably damaging	Het
Grid2ip	A	T	5: 143,386,015 (GRCm38)	E664D	possibly damaging	Het
Il1rn	A	G	2: 24,345,570 (GRCm38)	R21G	probably damaging	Het
Inpp4b	G	A	8: 81,890,753 (GRCm38)	G220R	probably benign	Het
Irx6	A	G	8: 92,678,253 (GRCm38)	S250G	possibly damaging	Het
Lyst	T	C	13: 13,634,483 (GRCm38)	I246T	probably benign	Het
Mcm3	G	A	1: 20,812,672 (GRCm38)	Q353*	probably null	Het
Mfhas1	A	G	8: 35,591,053 (GRCm38)	Y894C	probably damaging	Het
Mfsd13a	T	C	19: 46,372,053 (GRCm38)	L348P	probably damaging	Het
Necab1	A	G	4: 15,111,192 (GRCm38)		probably null	Het
Nectin3	A	T	16: 46,463,842 (GRCm38)	S160T	possibly damaging	Het
Npc2	A	G	12: 84,760,884 (GRCm38)	S67P	probably damaging	Het
Olfr1036	G	A	2: 86,074,820 (GRCm38)	V27M	probably benign	Het
Olfr513	T	G	7: 108,754,907 (GRCm38)	F17C	probably damaging	Het
Pcnx3	A	G	19: 5,677,182 (GRCm38)	F1108L	probably benign	Het
Phf21a	G	A	2: 92,359,199 (GRCm38)	S601N	probably benign	Het
Pold1	C	T	7: 44,535,051 (GRCm38)	V842I	probably benign	Het
Rabgap1	A	G	2: 37,543,234 (GRCm38)		probably null	Het
Setd1a	T	A	7: 127,797,424 (GRCm38)		probably benign	Het
Sgo2a	A	T	1: 57,999,962 (GRCm38)		probably null	Het
Sult2a8	T	A	7: 14,425,425 (GRCm38)	K90*	probably null	Het
Tlr2	T	C	3: 83,838,269 (GRCm38)	D169G	possibly damaging	Het
Tube1	G	T	10: 39,134,208 (GRCm38)	G10*	probably null	Het
Vmn2r10	T	C	5: 108,996,024 (GRCm38)	M687V	probably benign	Het
Zc3h8	G	A	2: 128,935,369 (GRCm38)	P117S	probably benign	Het
Zeb1	T	A	18: 5,705,089 (GRCm38)	D18E	probably damaging	Het

Other mutations in Tmem95

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R2001:Tmem95	UTSW	11	69,876,991 (GRCm38)	missense	probably damaging	0.97
R6533:Tmem95	UTSW	11	69,878,017 (GRCm38)	start codon destroyed	probably null	0.53
Z1177:Tmem95	UTSW	11	69,876,994 (GRCm38)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- GCTGGAAAAGCTGTCCTCCTCTTC -3'
(R):5'- GCTCCTGTCTGCCGTGAGTAAAAG -3'

Sequencing Primer
(F):5'- AAAGCTGTCCTCCTCTTCACAAG -3'
(R):5'- AACTGCTCCACCTGCGAG -3'

Posted On

2014-01-29