Mutagenetix > Incidental Mutation

Incidental Mutation 'R1236:Zscan26'

152440

Institutional Source

Beutler Lab

Gene Symbol

Zscan26

Ensembl Gene

ENSMUSG00000022228

Gene Name

zinc finger and SCAN domain containing 26

Synonyms

Zfp187

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.119) question?

Stock #

R1236 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

21442180-21453730 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 21445770 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Valine at position 188 (M188V)

Ref Sequence

ENSEMBL: ENSMUSP00000106111 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000032820] [ENSMUST00000110485] [ENSMUST00000122872] [ENSMUST00000148071] [ENSMUST00000151743]

AlphaFold

Q5RJ54

Predicted Effect

probably benign
Transcript: ENSMUST00000032820
AA Change: M62V

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000032820
Gene: ENSMUSG00000022228
AA Change: M62V

Domain	Start	End	E-Value	Type
ZnF_C2H2	144	166	1.58e-3	SMART
ZnF_C2H2	172	194	6.99e-5	SMART
ZnF_C2H2	200	222	1.67e-2	SMART
ZnF_C2H2	228	250	1.45e-2	SMART
ZnF_C2H2	256	278	2.53e-2	SMART
ZnF_C2H2	284	306	5.14e-3	SMART
ZnF_C2H2	312	334	2.95e-3	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000110485
AA Change: M188V

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000106111
Gene: ENSMUSG00000022228
AA Change: M188V

Domain	Start	End	E-Value	Type
SCAN	38	151	1.58e-48	SMART
ZnF_C2H2	270	292	1.58e-3	SMART
ZnF_C2H2	298	320	6.99e-5	SMART
ZnF_C2H2	326	348	1.67e-2	SMART
ZnF_C2H2	354	376	1.45e-2	SMART
ZnF_C2H2	382	404	2.53e-2	SMART
ZnF_C2H2	410	432	5.14e-3	SMART
ZnF_C2H2	438	460	2.95e-3	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000122641

Predicted Effect

probably benign
Transcript: ENSMUST00000122872

SMART Domains

Protein: ENSMUSP00000120175
Gene: ENSMUSG00000055313

Domain	Start	End	E-Value	Type
Blast:SCAN	1	65	1e-13	BLAST
low complexity region	155	167	N/A	INTRINSIC
Pfam:DDE_Tnp_1_7	213	374	1.4e-42	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000122926

Predicted Effect

probably benign
Transcript: ENSMUST00000148071

SMART Domains

Protein: ENSMUSP00000114882
Gene: ENSMUSG00000055313

Domain	Start	End	E-Value	Type
Blast:SCAN	1	65	3e-16	BLAST

Predicted Effect

probably benign
Transcript: ENSMUST00000151743

SMART Domains

Protein: ENSMUSP00000117669
Gene: ENSMUSG00000055313

Domain	Start	End	E-Value	Type
Blast:SCAN	1	65	7e-14	BLAST
low complexity region	192	204	N/A	INTRINSIC
Pfam:DDE_Tnp_1_7	250	411	1.7e-42	PFAM

Coding Region Coverage

1x: 99.1%
3x: 98.3%
10x: 96.3%
20x: 92.8%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 29 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700001O22Rik	T	C	2: 30,795,744	Y360C	probably damaging	Het
Afap1l2	T	A	19: 56,916,472	H566L	possibly damaging	Het
Aqr	G	T	2: 114,116,655	F1015L	probably damaging	Het
Atp5s	T	C	12: 69,741,818		probably null	Het
Cep112	T	A	11: 108,859,374	L901H	probably damaging	Het
Col26a1	A	G	5: 136,754,926	V229A	probably benign	Het
Cyp4a14	T	C	4: 115,492,170	N231S	probably benign	Het
G530012D18Rik	CAGAGAGA	CAGAGAGAGA	1: 85,577,224		probably null	Het
Gm37240	T	A	3: 84,527,696	N13I	probably benign	Het
Kbtbd7	G	T	14: 79,427,832	C368F	probably benign	Het
Kyat3	A	G	3: 142,738,259	D418G	probably benign	Het
Lpcat2	A	G	8: 92,886,569	M246V	probably damaging	Het
Nbas	T	A	12: 13,269,241	W31R	probably damaging	Het
Nckap1	C	T	2: 80,517,942	S889N	probably benign	Het
Npepl1	T	A	2: 174,114,480		probably null	Het
Olfr805	A	G	10: 129,722,806	V246A	probably damaging	Het
Olfr906	T	C	9: 38,488,229	S67P	probably damaging	Het
P4ha3	T	C	7: 100,293,849	L147P	probably damaging	Het
Pkp2	C	A	16: 16,225,902	H173Q	probably benign	Het
Prlr	C	A	15: 10,325,281	T180K	probably benign	Het
Psph	A	G	5: 129,771,476	M47T	probably damaging	Het
Rufy2	A	G	10: 62,994,770	N217S	probably benign	Het
Sgcg	T	C	14: 61,245,770	M61V	probably damaging	Het
Snrnp40	C	G	4: 130,378,043		probably null	Het
Spint1	A	G	2: 119,245,573	T217A	probably benign	Het
Tert	T	C	13: 73,636,379	L648P	probably damaging	Het
Vwde	A	T	6: 13,187,153	Y778*	probably null	Het
Zeb2	T	A	2: 44,994,646	D967V	probably damaging	Het
Zfp687	T	C	3: 95,012,044	N139S	probably benign	Het

Other mutations in Zscan26

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00503:Zscan26	APN	13	21445101	missense	probably damaging	0.99
IGL00720:Zscan26	APN	13	21445199	missense	probably benign	0.00
R0558:Zscan26	UTSW	13	21445055	missense	probably benign	0.37
R1912:Zscan26	UTSW	13	21445140	missense	possibly damaging	0.84
R4692:Zscan26	UTSW	13	21445257	missense	probably damaging	1.00
R5818:Zscan26	UTSW	13	21445761	missense	probably benign	0.00
R6089:Zscan26	UTSW	13	21448329	missense	probably damaging	0.97
R8029:Zscan26	UTSW	13	21445350	missense	probably damaging	1.00
R9448:Zscan26	UTSW	13	21448261	missense	probably benign
Z1088:Zscan26	UTSW	13	21445463	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CCTGATGGTCAGCAGCAACAGAAC -3'
(R):5'- TGCTGTAACTGTGGGACCTGAGAG -3'

Sequencing Primer
(F):5'- TGAGATCCTACAGATTCCCAGG -3'
(R):5'- gctcacaacattctgtaactcc -3'

Posted On

2014-01-29