Incidental Mutation 'V7581:Tspyl3'
ID 152537
Institutional Source Beutler Lab
Gene Symbol Tspyl3
Ensembl Gene ENSMUSG00000074671
Gene Name TSPY-like 3
Synonyms LOC241732
Accession Numbers
Is this an essential gene? Probably non essential (E-score: 0.089) question?
Stock # V7581 () of strain stinger
Quality Score 154
Status Not validated
Chromosome 2
Chromosomal Location 153222370-153225441 bp(-) (GRCm38)
Type of Mutation missense
DNA Base Change (assembly) A to G at 153225060 bp (GRCm38)
Zygosity Heterozygous
Amino Acid Change Valine to Alanine at position 86 (V86A)
Ref Sequence ENSEMBL: ENSMUSP00000096799 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000056924] [ENSMUST00000099194] [ENSMUST00000109795]
AlphaFold Q3UYP3
Predicted Effect probably benign
Transcript: ENSMUST00000056924
SMART Domains Protein: ENSMUSP00000055709
Gene: ENSMUSG00000051413

DomainStartEndE-ValueType
ZnF_C2H2 68 92 5.99e-4 SMART
ZnF_C2H2 98 120 1.03e-2 SMART
ZnF_C2H2 127 149 1.36e-2 SMART
ZnF_C2H2 156 178 6.23e-2 SMART
ZnF_C2H2 191 213 1.26e-2 SMART
ZnF_C2H2 219 242 3.11e-2 SMART
low complexity region 324 340 N/A INTRINSIC
low complexity region 369 397 N/A INTRINSIC
low complexity region 417 432 N/A INTRINSIC
low complexity region 443 458 N/A INTRINSIC
Predicted Effect probably benign
Transcript: ENSMUST00000099194
AA Change: V86A

PolyPhen 2 Score 0.070 (Sensitivity: 0.94; Specificity: 0.84)
SMART Domains Protein: ENSMUSP00000096799
Gene: ENSMUSG00000074671
AA Change: V86A

DomainStartEndE-ValueType
low complexity region 41 52 N/A INTRINSIC
low complexity region 76 90 N/A INTRINSIC
Pfam:NAP 160 307 2.9e-23 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000109795
SMART Domains Protein: ENSMUSP00000105419
Gene: ENSMUSG00000051413

DomainStartEndE-ValueType
ZnF_C2H2 68 92 5.99e-4 SMART
ZnF_C2H2 98 120 1.03e-2 SMART
ZnF_C2H2 127 149 1.36e-2 SMART
ZnF_C2H2 156 178 6.23e-2 SMART
ZnF_C2H2 191 213 1.26e-2 SMART
ZnF_C2H2 219 242 3.11e-2 SMART
low complexity region 324 340 N/A INTRINSIC
low complexity region 369 397 N/A INTRINSIC
low complexity region 417 432 N/A INTRINSIC
low complexity region 443 458 N/A INTRINSIC
Coding Region Coverage
  • 1x: 99.0%
  • 3x: 98.2%
  • 10x: 95.7%
  • 20x: 90.5%
Validation Efficiency
Allele List at MGI
Other mutations in this stock
Total: 46 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
9530002B09Rik A T 4: 122,701,257 H102L possibly damaging Het
Abcb10 GGCCATCG GG 8: 123,969,761 probably benign Het
Atp6v1h A G 1: 5,124,443 T282A possibly damaging Het
Clasp1 G A 1: 118,581,348 R1027Q probably damaging Het
D630003M21Rik T C 2: 158,201,011 T870A probably benign Het
Dnah12 T A 14: 26,773,093 N1369K possibly damaging Het
Dnajc22 T A 15: 99,101,482 Y183N probably damaging Het
Dpyd C T 3: 118,897,126 Q295* probably null Het
Erv3 T C 2: 131,855,926 H171R possibly damaging Het
Fam221b T C 4: 43,665,865 T249A probably benign Het
Fcgr1 T C 3: 96,284,276 *405W probably null Het
Glrx3 A G 7: 137,459,153 H172R probably benign Het
Gm10770 T A 2: 150,179,484 K38* probably null Het
Gm4787 G A 12: 81,377,567 Q606* probably null Het
Hira G A 16: 18,894,821 A29T probably damaging Het
Hnrnpab A T 11: 51,602,624 N252K probably benign Het
Izumo4 A T 10: 80,703,891 T155S probably benign Het
Kcnb2 A G 1: 15,710,091 I396V probably benign Het
Klc1 A T 12: 111,774,572 I161F probably benign Het
Lpar5 C A 6: 125,081,727 A137E possibly damaging Het
Lrp4 C T 2: 91,488,518 S900L possibly damaging Het
Muc6 T G 7: 141,647,613 E808A probably benign Het
Olfr1406 G T 1: 173,183,964 L157I probably benign Het
Olfr480 G T 7: 108,066,678 T40K probably benign Het
Otop3 T A 11: 115,344,838 L432Q probably damaging Het
Papln C T 12: 83,778,834 R608C possibly damaging Het
Pelp1 T A 11: 70,398,150 T257S probably damaging Het
Pik3cd A C 4: 149,657,319 L390R probably damaging Het
Ppwd1 A G 13: 104,220,237 Y257H probably damaging Het
Prkcb G T 7: 122,528,476 W274C probably damaging Het
Rbbp8nl T A 2: 180,278,208 T558S probably benign Het
Recql4 T C 15: 76,706,169 D705G possibly damaging Het
Slc30a4 T A 2: 122,689,538 M136L probably benign Het
Slc5a6 C T 5: 31,042,613 probably null Het
Spaca1 T C 4: 34,039,311 E192G probably damaging Het
Spata31 C A 13: 64,921,648 P537T probably benign Het
Tmc3 T C 7: 83,622,505 V955A probably benign Het
Tnrc6c G A 11: 117,723,326 R770H probably damaging Het
Toe1 A T 4: 116,806,111 N56K probably damaging Het
Tprkb A G 6: 85,928,782 K150E probably damaging Het
Trps1 T C 15: 50,831,577 K150E probably damaging Het
Vmn2r68 C T 7: 85,221,880 V732I probably benign Het
Wdr60 A C 12: 116,211,840 S906A possibly damaging Het
Zfp292 C T 4: 34,806,783 C2087Y possibly damaging Het
Zfp933 G A 4: 147,826,470 A223V probably damaging Het
Zmynd8 G A 2: 165,812,394 R724* probably null Het
Other mutations in Tspyl3
AlleleSourceChrCoordTypePredicted EffectPPH Score
F5770:Tspyl3 UTSW 2 153225060 missense probably benign 0.07
R0035:Tspyl3 UTSW 2 153224320 missense probably damaging 1.00
R0866:Tspyl3 UTSW 2 153224934 missense probably damaging 1.00
R1780:Tspyl3 UTSW 2 153225256 missense probably damaging 0.97
R1930:Tspyl3 UTSW 2 153224797 missense probably damaging 1.00
R3015:Tspyl3 UTSW 2 153224730 missense probably damaging 1.00
R4296:Tspyl3 UTSW 2 153225156 missense possibly damaging 0.96
R5913:Tspyl3 UTSW 2 153224716 missense probably benign 0.17
R6933:Tspyl3 UTSW 2 153225283 missense probably benign
V7580:Tspyl3 UTSW 2 153225060 missense probably benign 0.07
V7583:Tspyl3 UTSW 2 153225060 missense probably benign 0.07
Predicted Primers PCR Primer
(F):5'- TGCGAGTAGCAATGGACACCAC -3'
(R):5'- ATACAAGAGCGGGATGCACACC -3'

Sequencing Primer
(F):5'- AGGTAGCCAAGCATGTCTTC -3'
(R):5'- CGCCTTCCTCAGAAAACACT -3'
Posted On 2014-01-29