Mutagenetix > Incidental Mutations

Incidental Mutation 'R1081:Zfp770'

152680

Institutional Source

Beutler Lab

Gene Symbol

Zfp770

Ensembl Gene

ENSMUSG00000040321

Gene Name

zinc finger protein 770

Synonyms

6430601A21Rik

MMRRC Submission

039167-MU

Accession Numbers

Ncbi RefSeq: NM_175466.4; MGI: 2445100

Is this an essential gene?

Possibly non essential (E-score: 0.258) question?

Stock #

R1081 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

114193461-114201469 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 114197127 bp

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Histidine at position 154 (Y154H)

Ref Sequence

ENSEMBL: ENSMUSP00000052194 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000050668]

Predicted Effect

probably damaging
Transcript: ENSMUST00000050668
AA Change: Y154H

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000052194
Gene: ENSMUSG00000040321
AA Change: Y154H

Domain	Start	End	E-Value	Type
ZnF_C2H2	31	53	1.18e-2	SMART
ZnF_C2H2	59	81	4.11e-2	SMART
ZnF_C2H2	85	107	3.58e-2	SMART
ZnF_C2H2	164	186	2.09e-3	SMART
ZnF_C2H2	192	214	3.58e-2	SMART
ZnF_C2H2	220	242	3.29e-1	SMART
ZnF_C2H2	298	318	1.93e2	SMART
low complexity region	341	354	N/A	INTRINSIC
ZnF_C2H2	485	507	5.9e-3	SMART
ZnF_C2H2	513	535	1.82e-3	SMART
low complexity region	576	595	N/A	INTRINSIC
ZnF_C2H2	640	662	9.58e-3	SMART
ZnF_C2H2	668	690	7.37e-4	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000123562

Coding Region Coverage

1x: 99.0%
3x: 98.2%
10x: 96.0%
20x: 91.8%

Validation Efficiency

Allele List at MGI

All alleles(4) : Targeted(3) Gene trapped(1)

Other mutations in this stock

Total: 43 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700003E16Rik	A	G	6: 83,162,020	E309G	probably benign	Het
Abr	T	C	11: 76,455,615	K448E	probably damaging	Het
Atxn2l	T	C	7: 126,494,212	Y785C	probably damaging	Het
Atxn3	T	G	12: 101,934,349	D225A	probably damaging	Het
BC049730	T	C	7: 24,713,542		probably null	Het
Cdh15	G	C	8: 122,857,495	E112Q	probably damaging	Het
Cntnap5c	A	G	17: 58,305,525	D853G	possibly damaging	Het
Dnah9	A	G	11: 66,084,877	Y1449H	probably damaging	Het
Dsc2	T	G	18: 20,033,295	T760P	probably damaging	Het
Dync2h1	A	T	9: 7,005,488		probably null	Het
Epg5	T	C	18: 77,959,533	F611L	possibly damaging	Het
Fat3	T	C	9: 16,375,284	D981G	possibly damaging	Het
Gimap3	A	T	6: 48,765,152	C281*	probably null	Het
Ids	T	C	X: 70,361,110	D149G	possibly damaging	Het
Inpp4b	A	G	8: 82,069,024	I826V	probably damaging	Het
Kcnrg	C	A	14: 61,607,714	H68N	possibly damaging	Het
Klra6	A	G	6: 130,022,625	Y127H	probably damaging	Het
Mepce	A	G	5: 137,784,696	L456P	probably damaging	Het
Mink1	T	C	11: 70,607,035	L488P	probably benign	Het
Mndal	T	A	1: 173,860,222	E482V	probably benign	Het
Mob1b	G	A	5: 88,753,162	V143I	probably benign	Het
Msh4	T	C	3: 153,872,358	E433G	probably benign	Het
Myof	A	T	19: 37,986,088	I201N	probably damaging	Het
Naip1	G	A	13: 100,423,070	S1142F	probably benign	Het
Ntsr1	G	A	2: 180,538,756	S285N	probably benign	Het
Olfr319	C	T	11: 58,702,498	H266Y	probably damaging	Het
Olfr598	A	G	7: 103,329,038	Y184C	probably damaging	Het
Olfr66	T	A	7: 103,882,177	H22L	possibly damaging	Het
Olfr730	A	G	14: 50,187,197	S7P	probably damaging	Het
P2rx4	A	G	5: 122,727,233	E307G	probably damaging	Het
Pcdh15	A	G	10: 74,450,313	D793G	probably damaging	Het
Rpap1	G	A	2: 119,771,269	R737W	probably damaging	Het
Rpusd4	A	G	9: 35,275,088	K307E	probably benign	Het
Shtn1	T	A	19: 58,975,015	T623S	probably benign	Het
Sntg1	A	G	1: 8,445,119	C397R	possibly damaging	Het
Stat5a	T	C	11: 100,881,060	F646S	probably damaging	Het
Tacc2	A	G	7: 130,728,574	E196G	possibly damaging	Het
Tcf25	T	C	8: 123,381,473	V89A	probably benign	Het
Trim7	G	T	11: 48,849,705	V210L	probably damaging	Het
Vmn2r57	A	G	7: 41,428,211	M177T	possibly damaging	Het
Wwc2	G	A	8: 47,828,764		probably benign	Het
Zfp28	A	G	7: 6,389,780	I152V	possibly damaging	Het
Zfp628	A	G	7: 4,920,183	H468R	probably damaging	Het

Other mutations in Zfp770

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00090:Zfp770	APN	2	114195932	missense	probably benign	0.20
IGL00478:Zfp770	APN	2	114197465	missense	probably damaging	1.00
IGL01539:Zfp770	APN	2	114197093	missense	probably damaging	0.99
IGL01778:Zfp770	APN	2	114196238	missense	probably damaging	0.96
IGL02596:Zfp770	APN	2	114195827	missense	probably benign	0.11
IGL03227:Zfp770	APN	2	114197089	nonsense	probably null
R0057:Zfp770	UTSW	2	114197232	nonsense	probably null
R0057:Zfp770	UTSW	2	114197232	nonsense	probably null
R1446:Zfp770	UTSW	2	114197033	missense	probably damaging	0.99
R4477:Zfp770	UTSW	2	114196884	missense	probably damaging	1.00
R4597:Zfp770	UTSW	2	114196770	missense	possibly damaging	0.46
R4964:Zfp770	UTSW	2	114197387	missense	probably benign	0.24
R4966:Zfp770	UTSW	2	114197387	missense	probably benign	0.24
R5259:Zfp770	UTSW	2	114197193	missense	probably benign	0.00
R5440:Zfp770	UTSW	2	114196115	missense	probably benign	0.42
R5910:Zfp770	UTSW	2	114196232	nonsense	probably null
R5941:Zfp770	UTSW	2	114197546	missense	possibly damaging	0.83
R6074:Zfp770	UTSW	2	114196389	missense	possibly damaging	0.68
R6341:Zfp770	UTSW	2	114196759	missense	probably benign	0.14
R7181:Zfp770	UTSW	2	114197391	missense	probably damaging	1.00
R7288:Zfp770	UTSW	2	114195661	nonsense	probably null
R7935:Zfp770	UTSW	2	114196824	missense	probably benign	0.00
R8119:Zfp770	UTSW	2	114197027	missense	probably damaging	1.00
R8304:Zfp770	UTSW	2	114197410	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GGCAGGAAGACACTTGTGTCCATTG -3'
(R):5'- CAACTCGTTCATCTGGAGAGGCATC -3'

Sequencing Primer
(F):5'- AAAAGCATCCTGCTTTGCG -3'
(R):5'- CAGTTGTAATATTTGCCAACGCC -3'

Posted On

2014-01-29