Incidental Mutation 'R1081:Olfr66'
ID152698
Institutional Source Beutler Lab
Gene Symbol Olfr66
Ensembl Gene ENSMUSG00000058200
Gene Nameolfactory receptor 66
Synonyms5'[b]1, MOR1-3, GA_x6K02T2PBJ9-6620959-6620024
MMRRC Submission 039167-MU
Accession Numbers
Is this an essential gene? Probably non essential (E-score: 0.134) question?
Stock #R1081 (G1)
Quality Score225
Status Not validated
Chromosome7
Chromosomal Location103879443-103886252 bp(-) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) T to A at 103882177 bp
ZygosityHeterozygous
Amino Acid Change Histidine to Leucine at position 22 (H22L)
Ref Sequence ENSEMBL: ENSMUSP00000149058 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000079117] [ENSMUST00000216303]
Predicted Effect possibly damaging
Transcript: ENSMUST00000079117
AA Change: H22L

PolyPhen 2 Score 0.872 (Sensitivity: 0.83; Specificity: 0.93)
SMART Domains Protein: ENSMUSP00000078119
Gene: ENSMUSG00000058200
AA Change: H22L

DomainStartEndE-ValueType
Pfam:7tm_4 29 308 3.9e-113 PFAM
Pfam:7TM_GPCR_Srsx 32 300 9.7e-7 PFAM
Pfam:7tm_1 39 290 8.4e-20 PFAM
Predicted Effect noncoding transcript
Transcript: ENSMUST00000120890
Predicted Effect possibly damaging
Transcript: ENSMUST00000216303
AA Change: H22L

PolyPhen 2 Score 0.872 (Sensitivity: 0.83; Specificity: 0.93)
Coding Region Coverage
  • 1x: 99.0%
  • 3x: 98.2%
  • 10x: 96.0%
  • 20x: 91.8%
Validation Efficiency
MGI Phenotype FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]
Allele List at MGI
Other mutations in this stock
Total: 43 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
1700003E16Rik A G 6: 83,162,020 E309G probably benign Het
Abr T C 11: 76,455,615 K448E probably damaging Het
Atxn2l T C 7: 126,494,212 Y785C probably damaging Het
Atxn3 T G 12: 101,934,349 D225A probably damaging Het
BC049730 T C 7: 24,713,542 probably null Het
Cdh15 G C 8: 122,857,495 E112Q probably damaging Het
Cntnap5c A G 17: 58,305,525 D853G possibly damaging Het
Dnah9 A G 11: 66,084,877 Y1449H probably damaging Het
Dsc2 T G 18: 20,033,295 T760P probably damaging Het
Dync2h1 A T 9: 7,005,488 probably null Het
Epg5 T C 18: 77,959,533 F611L possibly damaging Het
Fat3 T C 9: 16,375,284 D981G possibly damaging Het
Gimap3 A T 6: 48,765,152 C281* probably null Het
Ids T C X: 70,361,110 D149G possibly damaging Het
Inpp4b A G 8: 82,069,024 I826V probably damaging Het
Kcnrg C A 14: 61,607,714 H68N possibly damaging Het
Klra6 A G 6: 130,022,625 Y127H probably damaging Het
Mepce A G 5: 137,784,696 L456P probably damaging Het
Mink1 T C 11: 70,607,035 L488P probably benign Het
Mndal T A 1: 173,860,222 E482V probably benign Het
Mob1b G A 5: 88,753,162 V143I probably benign Het
Msh4 T C 3: 153,872,358 E433G probably benign Het
Myof A T 19: 37,986,088 I201N probably damaging Het
Naip1 G A 13: 100,423,070 S1142F probably benign Het
Ntsr1 G A 2: 180,538,756 S285N probably benign Het
Olfr319 C T 11: 58,702,498 H266Y probably damaging Het
Olfr598 A G 7: 103,329,038 Y184C probably damaging Het
Olfr730 A G 14: 50,187,197 S7P probably damaging Het
P2rx4 A G 5: 122,727,233 E307G probably damaging Het
Pcdh15 A G 10: 74,450,313 D793G probably damaging Het
Rpap1 G A 2: 119,771,269 R737W probably damaging Het
Rpusd4 A G 9: 35,275,088 K307E probably benign Het
Shtn1 T A 19: 58,975,015 T623S probably benign Het
Sntg1 A G 1: 8,445,119 C397R possibly damaging Het
Stat5a T C 11: 100,881,060 F646S probably damaging Het
Tacc2 A G 7: 130,728,574 E196G possibly damaging Het
Tcf25 T C 8: 123,381,473 V89A probably benign Het
Trim7 G T 11: 48,849,705 V210L probably damaging Het
Vmn2r57 A G 7: 41,428,211 M177T possibly damaging Het
Wwc2 G A 8: 47,828,764 probably benign Het
Zfp28 A G 7: 6,389,780 I152V possibly damaging Het
Zfp628 A G 7: 4,920,183 H468R probably damaging Het
Zfp770 A G 2: 114,197,127 Y154H probably damaging Het
Other mutations in Olfr66
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL02342:Olfr66 APN 7 103881380 missense probably benign 0.16
IGL02935:Olfr66 APN 7 103881664 missense probably damaging 1.00
IGL03033:Olfr66 APN 7 103881517 missense probably damaging 1.00
IGL03105:Olfr66 APN 7 103882132 missense possibly damaging 0.55
R1780:Olfr66 UTSW 7 103881592 missense probably benign 0.00
R4470:Olfr66 UTSW 7 103881749 missense probably benign 0.02
R4724:Olfr66 UTSW 7 103881649 missense probably benign 0.02
R5463:Olfr66 UTSW 7 103881334 missense probably benign
R5498:Olfr66 UTSW 7 103881632 missense probably damaging 0.97
R5987:Olfr66 UTSW 7 103881700 missense probably damaging 0.99
R6054:Olfr66 UTSW 7 103881826 missense probably damaging 0.98
R7815:Olfr66 UTSW 7 103881785 missense probably benign 0.16
R8026:Olfr66 UTSW 7 103881697 missense possibly damaging 0.96
Predicted Primers PCR Primer
(F):5'- TCGATCATAAGACATGGCAAGCAAGAC -3'
(R):5'- GTGACCTGAAGACATGCTACCATGAG -3'

Sequencing Primer
(F):5'- CCCGATTCTACAATGGCTAGTGAG -3'
(R):5'- CTACCATGAGGTGGCAGTAGG -3'
Posted On2014-01-29