Mutagenetix > Incidental Mutation

Incidental Mutation 'R1329:Vmn2r116'

156128

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r116

Ensembl Gene

ENSMUSG00000090966

Gene Name

vomeronasal 2, receptor 116

Synonyms

V2Rp5, EG619697

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.062) question?

Stock #

R1329 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

23384803-23401864 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 23387188 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Serine at position 358 (N358S)

Ref Sequence

ENSEMBL: ENSMUSP00000128106 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000164856]

AlphaFold

E9Q6I0

Predicted Effect

possibly damaging
Transcript: ENSMUST00000164856
AA Change: N358S

PolyPhen 2 Score 0.893 (Sensitivity: 0.82; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000128106
Gene: ENSMUSG00000090966
AA Change: N358S

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	73	469	4.4e-30	PFAM
Pfam:NCD3G	511	564	1.2e-22	PFAM
low complexity region	589	594	N/A	INTRINSIC
Pfam:7tm_3	595	832	8.7e-57	PFAM

Coding Region Coverage

1x: 99.0%
3x: 98.2%
10x: 96.1%
20x: 92.5%

Validation Efficiency

MGI Phenotype

PHENOTYPE: Female mice homozygous for a knock-out allele stimulated with male pheromone (Gm6084) fail to exhibit an increase in lordosis behavior and successful intromission. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 25 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acot7	C	T	4: 152,229,784 (GRCm38)	Q188*	probably null	Het
Ano5	T	C	7: 51,546,785 (GRCm38)	Y141H	probably benign	Het
Atg101	G	A	15: 101,290,290 (GRCm38)	G92D	probably null	Het
Brwd1	A	G	16: 96,003,234 (GRCm38)	I1912T	probably benign	Het
Cad	G	T	5: 31,059,582 (GRCm38)	G263W	probably damaging	Het
Clstn2	T	C	9: 97,458,174 (GRCm38)	E715G	probably damaging	Het
Cracd	A	G	5: 76,657,932 (GRCm38)		probably benign	Het
Dbp	C	T	7: 45,708,328 (GRCm38)	P70S	probably damaging	Het
Fem1al	T	A	11: 29,823,553 (GRCm38)	N635Y	probably benign	Het
Gpr61	T	A	3: 108,150,514 (GRCm38)	H277L	probably benign	Het
Gsdma3	G	T	11: 98,632,392 (GRCm38)	V203F	probably damaging	Het
Ifih1	G	C	2: 62,617,487 (GRCm38)		probably null	Het
Myo1e	T	A	9: 70,338,738 (GRCm38)	C404S	possibly damaging	Het
Myrfl	A	G	10: 116,777,342 (GRCm38)		probably null	Het
Nfat5	T	G	8: 107,369,027 (GRCm38)	M1300R	probably benign	Het
Nfrkb	C	T	9: 31,414,647 (GRCm38)	P1129S	possibly damaging	Het
Nubp2	A	T	17: 24,883,864 (GRCm38)	N208K	possibly damaging	Het
Or8g20	A	G	9: 39,484,444 (GRCm38)	S270P	probably damaging	Het
Ovch2	T	C	7: 107,785,446 (GRCm38)	D488G	probably damaging	Het
Rfx6	A	T	10: 51,693,737 (GRCm38)	Y202F	probably damaging	Het
Rin2	C	T	2: 145,860,446 (GRCm38)	T354I	probably benign	Het
Usp4	T	A	9: 108,372,566 (GRCm38)	V431E	probably damaging	Het
Vil1	A	G	1: 74,427,558 (GRCm38)	I636V	probably benign	Het
Wdr47	T	A	3: 108,627,299 (GRCm38)	N511K	probably benign	Het
Wdr5	T	A	2: 27,531,671 (GRCm38)	F222I	probably damaging	Het

Other mutations in Vmn2r116

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00898:Vmn2r116	APN	17	23,385,995 (GRCm38)	missense	possibly damaging	0.94
IGL00985:Vmn2r116	APN	17	23,401,515 (GRCm38)	missense	probably damaging	1.00
IGL00990:Vmn2r116	APN	17	23,387,236 (GRCm38)	missense	probably benign	0.12
IGL00990:Vmn2r116	APN	17	23,397,727 (GRCm38)	missense	probably damaging	1.00
IGL01383:Vmn2r116	APN	17	23,401,601 (GRCm38)	missense	probably damaging	1.00
IGL01459:Vmn2r116	APN	17	23,384,929 (GRCm38)	missense	probably damaging	1.00
IGL01725:Vmn2r116	APN	17	23,386,645 (GRCm38)	missense	probably damaging	1.00
IGL02125:Vmn2r116	APN	17	23,397,627 (GRCm38)	splice site	probably benign
IGL02170:Vmn2r116	APN	17	23,384,933 (GRCm38)	missense	probably benign
IGL02209:Vmn2r116	APN	17	23,388,787 (GRCm38)	missense	probably damaging	1.00
IGL02226:Vmn2r116	APN	17	23,384,834 (GRCm38)	missense	probably null
IGL02272:Vmn2r116	APN	17	23,386,004 (GRCm38)	missense	probably damaging	1.00
IGL02272:Vmn2r116	APN	17	23,385,999 (GRCm38)	missense	probably benign	0.06
IGL02403:Vmn2r116	APN	17	23,387,364 (GRCm38)	missense	probably damaging	1.00
IGL02686:Vmn2r116	APN	17	23,388,793 (GRCm38)	missense	probably damaging	0.99
IGL02750:Vmn2r116	APN	17	23,397,634 (GRCm38)	splice site	probably benign
IGL02977:Vmn2r116	APN	17	23,388,774 (GRCm38)	missense	possibly damaging	0.90
PIT4449001:Vmn2r116	UTSW	17	23,388,947 (GRCm38)	missense	probably benign	0.41
R0015:Vmn2r116	UTSW	17	23,401,849 (GRCm38)	missense	probably benign	0.03
R0219:Vmn2r116	UTSW	17	23,386,098 (GRCm38)	nonsense	probably null
R0281:Vmn2r116	UTSW	17	23,401,413 (GRCm38)	missense	possibly damaging	0.90
R0415:Vmn2r116	UTSW	17	23,387,279 (GRCm38)	missense	possibly damaging	0.55
R0592:Vmn2r116	UTSW	17	23,386,915 (GRCm38)	missense	probably damaging	0.99
R0610:Vmn2r116	UTSW	17	23,387,312 (GRCm38)	missense	probably damaging	1.00
R0635:Vmn2r116	UTSW	17	23,386,887 (GRCm38)	missense	possibly damaging	0.95
R0843:Vmn2r116	UTSW	17	23,400,960 (GRCm38)	missense	probably benign	0.01
R1396:Vmn2r116	UTSW	17	23,386,141 (GRCm38)	missense	probably benign
R1401:Vmn2r116	UTSW	17	23,386,596 (GRCm38)	splice site	probably benign
R1574:Vmn2r116	UTSW	17	23,387,089 (GRCm38)	missense	probably damaging	0.99
R1574:Vmn2r116	UTSW	17	23,387,089 (GRCm38)	missense	probably damaging	0.99
R1766:Vmn2r116	UTSW	17	23,401,766 (GRCm38)	missense	probably damaging	0.98
R2157:Vmn2r116	UTSW	17	23,401,469 (GRCm38)	missense	probably damaging	1.00
R3622:Vmn2r116	UTSW	17	23,386,051 (GRCm38)	missense	probably benign	0.11
R3690:Vmn2r116	UTSW	17	23,384,824 (GRCm38)	missense	unknown
R4298:Vmn2r116	UTSW	17	23,401,827 (GRCm38)	missense	possibly damaging	0.69
R4373:Vmn2r116	UTSW	17	23,401,421 (GRCm38)	missense	probably benign	0.01
R4860:Vmn2r116	UTSW	17	23,401,803 (GRCm38)	missense	probably benign
R4941:Vmn2r116	UTSW	17	23,401,142 (GRCm38)	missense	probably damaging	1.00
R5119:Vmn2r116	UTSW	17	23,387,164 (GRCm38)	missense	probably benign	0.01
R5503:Vmn2r116	UTSW	17	23,386,804 (GRCm38)	missense	probably benign	0.07
R5510:Vmn2r116	UTSW	17	23,386,121 (GRCm38)	missense	probably damaging	1.00
R5538:Vmn2r116	UTSW	17	23,401,067 (GRCm38)	missense	probably benign	0.00
R5689:Vmn2r116	UTSW	17	23,397,719 (GRCm38)	missense	probably benign	0.30
R5765:Vmn2r116	UTSW	17	23,401,404 (GRCm38)	missense	probably damaging	0.99
R5794:Vmn2r116	UTSW	17	23,385,968 (GRCm38)	missense	probably damaging	0.99
R5807:Vmn2r116	UTSW	17	23,387,307 (GRCm38)	missense	probably damaging	1.00
R5837:Vmn2r116	UTSW	17	23,387,080 (GRCm38)	missense	probably damaging	1.00
R6262:Vmn2r116	UTSW	17	23,387,377 (GRCm38)	missense	probably benign	0.03
R6298:Vmn2r116	UTSW	17	23,386,762 (GRCm38)	missense	probably damaging	1.00
R6651:Vmn2r116	UTSW	17	23,388,831 (GRCm38)	nonsense	probably null
R6667:Vmn2r116	UTSW	17	23,401,092 (GRCm38)	missense	probably damaging	1.00
R7393:Vmn2r116	UTSW	17	23,386,125 (GRCm38)	missense	probably benign	0.14
R7571:Vmn2r116	UTSW	17	23,384,856 (GRCm38)	splice site	probably null
R7940:Vmn2r116	UTSW	17	23,386,972 (GRCm38)	missense	probably damaging	0.99
R8510:Vmn2r116	UTSW	17	23,385,931 (GRCm38)	nonsense	probably null
R8950:Vmn2r116	UTSW	17	23,401,493 (GRCm38)	missense	probably damaging	1.00
R8956:Vmn2r116	UTSW	17	23,386,762 (GRCm38)	missense	probably damaging	1.00
R8977:Vmn2r116	UTSW	17	23,386,942 (GRCm38)	missense	possibly damaging	0.56
R9030:Vmn2r116	UTSW	17	23,384,890 (GRCm38)	missense	possibly damaging	0.82
R9077:Vmn2r116	UTSW	17	23,385,982 (GRCm38)	missense	probably benign	0.14
R9223:Vmn2r116	UTSW	17	23,401,167 (GRCm38)	missense	probably damaging	1.00
R9401:Vmn2r116	UTSW	17	23,401,592 (GRCm38)	missense	probably damaging	1.00
R9449:Vmn2r116	UTSW	17	23,386,945 (GRCm38)	missense	probably benign	0.01
R9746:Vmn2r116	UTSW	17	23,401,823 (GRCm38)	missense	probably benign	0.08
R9755:Vmn2r116	UTSW	17	23,401,091 (GRCm38)	missense	probably damaging	1.00
R9759:Vmn2r116	UTSW	17	23,401,386 (GRCm38)	missense	possibly damaging	0.90
R9800:Vmn2r116	UTSW	17	23,401,425 (GRCm38)	missense	probably damaging	0.97
S24628:Vmn2r116	UTSW	17	23,387,279 (GRCm38)	missense	possibly damaging	0.55
Z1176:Vmn2r116	UTSW	17	23,401,428 (GRCm38)	missense	probably damaging	1.00
Z1177:Vmn2r116	UTSW	17	23,388,892 (GRCm38)	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- GGAACTTTTCATCCTGCCCTGAGTG -3'
(R):5'- GTACATGGGCAAAAGCATACACAGC -3'

Sequencing Primer
(F):5'- ACAGTACCTTAGCTGTGAGC -3'
(R):5'- GGATGAGCAGTTCTTCAGTGTCTT -3'

Posted On

2014-02-11