Mutagenetix > Incidental Mutations

Incidental Mutation 'R1355:Olfr1466'

156308

Institutional Source

Beutler Lab

Gene Symbol

Olfr1466

Ensembl Gene

ENSMUSG00000096485

Gene Name

olfactory receptor 1466

Synonyms

MOR202-12, GA_x6K02T2RE5P-3672907-3673839

MMRRC Submission

039420-MU

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.075) question?

Stock #

R1355 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

13338862-13343572 bp(+) (GRCm38)

Type of Mutation

nonsense

DNA Base Change (assembly)

T to A at 13342518 bp

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Stop codon at position 253 (Y253*)

Ref Sequence

ENSEMBL: ENSMUSP00000147188 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000075868] [ENSMUST00000207124]

Predicted Effect

probably null
Transcript: ENSMUST00000075868
AA Change: Y253*

SMART Domains

Protein: ENSMUSP00000075265
Gene: ENSMUSG00000096485
AA Change: Y253*

Domain	Start	End	E-Value	Type
Pfam:7tm_4	32	310	4.1e-49	PFAM
Pfam:7TM_GPCR_Srsx	36	306	1.9e-6	PFAM
Pfam:7tm_1	42	291	7e-19	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000207124
AA Change: Y253*

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 99.0%
3x: 98.1%
10x: 95.8%
20x: 91.4%

Validation Efficiency

100% (57/57)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 54 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcb10	C	T	8: 123,962,052	G495D	probably damaging	Het
Ankrd52	A	G	10: 128,388,696	D781G	possibly damaging	Het
Arhgef5	T	A	6: 43,283,912	F1424I	probably damaging	Het
Atp10b	G	A	11: 43,151,655	W14*	probably null	Het
B4galnt4	T	C	7: 141,065,395	V259A	probably damaging	Het
Ccdc141	A	T	2: 77,030,601	I944N	probably damaging	Het
Ccdc146	A	T	5: 21,321,242	D224E	probably damaging	Het
Ccne1	A	G	7: 38,106,322	I43T	possibly damaging	Het
Cd226	T	A	18: 89,247,023	S29T	probably benign	Het
Cebpz	G	T	17: 78,935,324	D300E	probably benign	Het
Cryzl1	A	G	16: 91,692,658	V266A	possibly damaging	Het
Cyp2c68	A	G	19: 39,740,956	L29P	probably damaging	Het
Dennd3	A	C	15: 73,540,854		probably benign	Het
Dpy19l4	C	T	4: 11,303,371	W183*	probably null	Het
Eml6	T	G	11: 29,833,085	S599R	probably benign	Het
Erc1	A	T	6: 119,743,420	L440*	probably null	Het
Fam71e1	A	G	7: 44,496,691	K2E	possibly damaging	Het
Frem3	A	G	8: 80,690,702	Y2012C	probably damaging	Het
Gm10288	A	T	3: 146,838,993		noncoding transcript	Het
Gm1110	T	A	9: 26,883,761	K476N	probably benign	Het
Gm11937	A	T	11: 99,609,907	S95T	possibly damaging	Het
Hist1h2bl	T	A	13: 21,715,857	Q96L	probably damaging	Het
Hs6st1	T	A	1: 36,103,576	H197Q	probably damaging	Het
Ism1	A	T	2: 139,732,074	I115F	possibly damaging	Het
Itgb8	C	T	12: 119,171,003	G443E	probably benign	Het
Kalrn	T	A	16: 33,975,584	I1274F	possibly damaging	Het
Lrrcc1	G	T	3: 14,548,114	V299L	probably benign	Het
Mettl5	A	T	2: 69,881,420		probably null	Het
Nlrp2	G	T	7: 5,327,491	N635K	possibly damaging	Het
Olfr140	G	A	2: 90,051,613	T237I	probably benign	Het
Olfr1537	G	C	9: 39,238,251	P58A	probably benign	Het
Olfr198	A	G	16: 59,201,680	S249P	probably damaging	Het
Plxna1	A	G	6: 89,320,766		probably benign	Het
Ppp4r1	A	T	17: 65,840,987	E924D	probably benign	Het
Prdm2	C	T	4: 143,131,963	V1586I	probably benign	Het
Pros1	A	G	16: 62,919,558	K457E	probably benign	Het
Rer1	T	A	4: 155,075,624	M156L	probably benign	Het
Rgs16	A	T	1: 153,743,668	K140M	probably damaging	Het
Rgsl1	A	G	1: 153,807,761	M1T	probably null	Het
Setdb2	T	A	14: 59,417,441	K333N	probably damaging	Het
Sgo2a	A	G	1: 58,017,965	T1103A	possibly damaging	Het
Sik3	C	T	9: 46,195,872		probably benign	Het
Slc44a3	C	A	3: 121,531,671	G47V	probably damaging	Het
Snrpd1	G	A	18: 10,627,818	G103D	probably benign	Het
Soga3	A	T	10: 29,147,322	T222S	probably benign	Het
Sspo	T	A	6: 48,448,626	S60R	probably benign	Het
Susd1	C	T	4: 59,424,114	C37Y	possibly damaging	Het
Tiam1	A	T	16: 89,898,221	I116N	probably benign	Het
Ttc3	A	G	16: 94,418,637	S492G	possibly damaging	Het
Uckl1	G	T	2: 181,573,376	T213K	probably damaging	Het
Usp38	T	C	8: 80,985,033	E791G	possibly damaging	Het
Vps13b	C	T	15: 35,422,454	R187C	probably damaging	Het
Vwa3a	A	G	7: 120,784,111	Y645C	probably damaging	Het
Wasf3	T	C	5: 146,470,208		probably benign	Het

Other mutations in Olfr1466

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02442:Olfr1466	APN	19	13342120	missense	probably benign	0.13
IGL02568:Olfr1466	APN	19	13342219	missense	probably benign	0.08
IGL03073:Olfr1466	APN	19	13342022	missense	probably benign	0.00
R0943:Olfr1466	UTSW	19	13341793	missense	probably benign	0.00
R1301:Olfr1466	UTSW	19	13341847	missense	probably benign	0.05
R1524:Olfr1466	UTSW	19	13342122	nonsense	probably null
R1568:Olfr1466	UTSW	19	13342175	missense	probably benign	0.14
R1993:Olfr1466	UTSW	19	13341814	missense	possibly damaging	0.65
R2031:Olfr1466	UTSW	19	13342406	missense	probably benign	0.18
R3693:Olfr1466	UTSW	19	13342529	missense	possibly damaging	0.73
R3694:Olfr1466	UTSW	19	13342529	missense	possibly damaging	0.73
R3853:Olfr1466	UTSW	19	13342498	missense	possibly damaging	0.55
R5313:Olfr1466	UTSW	19	13342065	missense	probably benign	0.07
R5467:Olfr1466	UTSW	19	13342157	missense	probably damaging	1.00
R6060:Olfr1466	UTSW	19	13342133	missense	probably benign	0.08
R7125:Olfr1466	UTSW	19	13341739	critical splice acceptor site	probably null
R7591:Olfr1466	UTSW	19	13342255	missense	probably benign	0.28
Z1177:Olfr1466	UTSW	19	13342255	missense	probably benign	0.28

Predicted Primers

PCR Primer
(F):5'- TGCCAGCAATCATGGCTCTCTC -3'
(R):5'- TGTTCCTTCATGTCCAGGCCAAAATG -3'

Sequencing Primer
(F):5'- GGCTCTCTCTTGCTCTGATAGAC -3'
(R):5'- GTCCAGGCCAAAATGATATTCATAGG -3'

Posted On

2014-02-11