Mutagenetix > Incidental Mutation

Incidental Mutation 'R1346:Etl4'

156492

Institutional Source

Beutler Lab

Gene Symbol

Etl4

Ensembl Gene

ENSMUSG00000036617

Gene Name

enhancer trap locus 4

Synonyms

6620402G01Rik, Sickle tail, E330027G05Rik, Etl-4, Skt, 9430077C05Rik

MMRRC Submission

039411-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.809) question?

Stock #

R1346 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

19915018-20815346 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 20810955 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Serine to Proline at position 1013 (S1013P)

Ref Sequence

ENSEMBL: ENSMUSP00000110255 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000045555] [ENSMUST00000066509] [ENSMUST00000114604] [ENSMUST00000114606] [ENSMUST00000114607] [ENSMUST00000114608] [ENSMUST00000114614] [ENSMUST00000114627]

AlphaFold

A2AQ25

Predicted Effect

probably benign
Transcript: ENSMUST00000045555

SMART Domains

Protein: ENSMUSP00000041431
Gene: ENSMUSG00000036617

Domain	Start	End	E-Value	Type
Pfam:AIP3	188	291	1.7e-11	PFAM
low complexity region	313	328	N/A	INTRINSIC
low complexity region	350	368	N/A	INTRINSIC
coiled coil region	620	652	N/A	INTRINSIC
low complexity region	1067	1096	N/A	INTRINSIC
low complexity region	1212	1231	N/A	INTRINSIC
low complexity region	1296	1314	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000066509
AA Change: S1330P

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000066170
Gene: ENSMUSG00000036617
AA Change: S1330P

Domain	Start	End	E-Value	Type
low complexity region	313	328	N/A	INTRINSIC
low complexity region	350	368	N/A	INTRINSIC
coiled coil region	655	687	N/A	INTRINSIC
low complexity region	1102	1131	N/A	INTRINSIC
low complexity region	1372	1381	N/A	INTRINSIC
low complexity region	1470	1495	N/A	INTRINSIC
low complexity region	1571	1582	N/A	INTRINSIC
coiled coil region	1658	1686	N/A	INTRINSIC
low complexity region	1724	1737	N/A	INTRINSIC
low complexity region	1806	1825	N/A	INTRINSIC
low complexity region	1890	1908	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000114604

SMART Domains

Protein: ENSMUSP00000110251
Gene: ENSMUSG00000036617

Domain	Start	End	E-Value	Type
Pfam:AIP3	188	291	1.7e-11	PFAM
low complexity region	313	328	N/A	INTRINSIC
low complexity region	350	368	N/A	INTRINSIC
coiled coil region	655	687	N/A	INTRINSIC
low complexity region	1102	1131	N/A	INTRINSIC
low complexity region	1207	1226	N/A	INTRINSIC
low complexity region	1291	1309	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000114606

SMART Domains

Protein: ENSMUSP00000110253
Gene: ENSMUSG00000036617

Domain	Start	End	E-Value	Type
low complexity region	31	46	N/A	INTRINSIC
low complexity region	68	86	N/A	INTRINSIC
coiled coil region	338	370	N/A	INTRINSIC
low complexity region	785	814	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000114607

SMART Domains

Protein: ENSMUSP00000110254
Gene: ENSMUSG00000036617

Domain	Start	End	E-Value	Type
low complexity region	31	46	N/A	INTRINSIC
low complexity region	68	86	N/A	INTRINSIC
coiled coil region	338	370	N/A	INTRINSIC
low complexity region	785	814	N/A	INTRINSIC

Predicted Effect

possibly damaging
Transcript: ENSMUST00000114608
AA Change: S1013P

PolyPhen 2 Score 0.945 (Sensitivity: 0.80; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000110255
Gene: ENSMUSG00000036617
AA Change: S1013P

Domain	Start	End	E-Value	Type
low complexity region	31	46	N/A	INTRINSIC
low complexity region	68	86	N/A	INTRINSIC
coiled coil region	338	370	N/A	INTRINSIC
low complexity region	785	814	N/A	INTRINSIC
low complexity region	1055	1064	N/A	INTRINSIC
low complexity region	1153	1178	N/A	INTRINSIC
low complexity region	1254	1265	N/A	INTRINSIC
coiled coil region	1341	1369	N/A	INTRINSIC
low complexity region	1407	1420	N/A	INTRINSIC
low complexity region	1489	1508	N/A	INTRINSIC
low complexity region	1573	1591	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000114614

SMART Domains

Protein: ENSMUSP00000110261
Gene: ENSMUSG00000036617

Domain	Start	End	E-Value	Type
Pfam:AIP3	188	291	1.7e-11	PFAM
low complexity region	313	328	N/A	INTRINSIC
low complexity region	350	368	N/A	INTRINSIC
coiled coil region	620	652	N/A	INTRINSIC
low complexity region	1056	1085	N/A	INTRINSIC
low complexity region	1201	1220	N/A	INTRINSIC
low complexity region	1285	1303	N/A	INTRINSIC

Predicted Effect

possibly damaging
Transcript: ENSMUST00000114627
AA Change: S1381P

PolyPhen 2 Score 0.465 (Sensitivity: 0.89; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000110274
Gene: ENSMUSG00000036617
AA Change: S1381P

Domain	Start	End	E-Value	Type
low complexity region	21	38	N/A	INTRINSIC
low complexity region	60	72	N/A	INTRINSIC
Pfam:AIP3	239	341	2.4e-14	PFAM
low complexity region	364	379	N/A	INTRINSIC
low complexity region	401	419	N/A	INTRINSIC
Pfam:AIP3	600	841	1.1e-12	PFAM
low complexity region	1153	1182	N/A	INTRINSIC
low complexity region	1423	1432	N/A	INTRINSIC
low complexity region	1521	1546	N/A	INTRINSIC
low complexity region	1622	1633	N/A	INTRINSIC
coiled coil region	1709	1737	N/A	INTRINSIC
low complexity region	1775	1788	N/A	INTRINSIC
low complexity region	1857	1876	N/A	INTRINSIC
low complexity region	1941	1959	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000125772

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000139531

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000156587

Meta Mutation Damage Score

0.1795

Coding Region Coverage

1x: 98.9%
3x: 97.9%
10x: 95.2%
20x: 90.1%

Validation Efficiency

98% (43/44)

MGI Phenotype

PHENOTYPE: Mice homozygous for a gene-trapped allele display malformations of the notochord and caudal vertebrae and may exhibit caudal tail kinks. Mice homozygous for another gene-trapped allele have malformed caudal vertebrae and intervertebral disk abnormalities; about half display kinked tails. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 38 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
3425401B19Rik	C	A	14: 32,382,771 (GRCm39)	A1065S	probably benign	Het
Ak5	G	T	3: 152,239,071 (GRCm39)	D301E	probably damaging	Het
Akap13	G	A	7: 75,259,340 (GRCm39)	G655S	possibly damaging	Het
Arap3	A	T	18: 38,108,971 (GRCm39)	C1228S	probably damaging	Het
Arfgef1	T	C	1: 10,229,958 (GRCm39)	T1248A	probably benign	Het
Atf7ip2	G	A	16: 10,052,195 (GRCm39)	V225I	probably damaging	Het
Bdp1	G	A	13: 100,215,263 (GRCm39)	Q374*	probably null	Het
Cacng6	G	T	7: 3,483,438 (GRCm39)	W255C	possibly damaging	Het
Camta2	T	C	11: 70,567,293 (GRCm39)	K628R	possibly damaging	Het
Catsperg1	A	T	7: 28,881,759 (GRCm39)		probably null	Het
Cers4	A	G	8: 4,565,632 (GRCm39)	E26G	probably damaging	Het
Chfr	A	G	5: 110,288,313 (GRCm39)	D76G	probably damaging	Het
Chrng	C	A	1: 87,135,985 (GRCm39)	Q245K	probably benign	Het
Cnn2	T	C	10: 79,829,414 (GRCm39)		probably benign	Het
Dyrk2	T	C	10: 118,695,624 (GRCm39)	K545E	possibly damaging	Het
Eif3d	A	G	15: 77,852,754 (GRCm39)	I9T	probably damaging	Het
Elovl7	A	G	13: 108,410,883 (GRCm39)	I153V	probably benign	Het
Furin	C	T	7: 80,041,932 (GRCm39)		probably benign	Het
Gart	G	T	16: 91,425,070 (GRCm39)		probably null	Het
Gm572	G	A	4: 148,739,354 (GRCm39)	V61M	possibly damaging	Het
Hspbap1	G	A	16: 35,622,035 (GRCm39)	A127T	probably damaging	Het
Kcnh2	T	C	5: 24,527,658 (GRCm39)	D898G	possibly damaging	Het
Kcnrg	A	G	14: 61,849,144 (GRCm39)	T202A	probably benign	Het
Lhx1	T	C	11: 84,412,905 (GRCm39)	E36G	possibly damaging	Het
Lrp1	T	C	10: 127,441,735 (GRCm39)	N167S	probably damaging	Het
Parp9	A	T	16: 35,777,267 (GRCm39)	M171L	probably benign	Het
Pla2g4e	G	A	2: 120,013,253 (GRCm39)	R356W	probably damaging	Het
Ppp4c	C	T	7: 126,391,222 (GRCm39)		probably benign	Het
Rab3c	G	A	13: 110,397,120 (GRCm39)	R49C	probably damaging	Het
Rbm25	T	C	12: 83,691,167 (GRCm39)		probably benign	Het
Sema4g	T	A	19: 44,986,091 (GRCm39)	S311T	possibly damaging	Het
Skida1	T	C	2: 18,053,090 (GRCm39)	I21V	possibly damaging	Het
Slc25a32	T	A	15: 38,963,411 (GRCm39)	I137F	probably benign	Het
Stard9	T	C	2: 120,543,929 (GRCm39)	V4409A	probably damaging	Het
Stx2	G	A	5: 129,065,852 (GRCm39)		probably benign	Het
Timeless	C	T	10: 128,078,234 (GRCm39)	T248M	possibly damaging	Het
Tlr2	T	A	3: 83,743,900 (GRCm39)	N728Y	probably damaging	Het
Zfp592	A	T	7: 80,687,812 (GRCm39)	N913Y	possibly damaging	Het

Other mutations in Etl4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00907:Etl4	APN	2	20,771,289 (GRCm39)	missense	possibly damaging	0.81
IGL00944:Etl4	APN	2	20,534,865 (GRCm39)	missense	possibly damaging	0.52
IGL01078:Etl4	APN	2	20,811,342 (GRCm39)	nonsense	probably null
IGL01099:Etl4	APN	2	20,811,922 (GRCm39)	missense	probably benign	0.06
IGL01337:Etl4	APN	2	20,790,198 (GRCm39)	missense	probably benign	0.01
IGL01348:Etl4	APN	2	20,811,784 (GRCm39)	missense	probably damaging	1.00
IGL01349:Etl4	APN	2	20,718,207 (GRCm39)	missense	probably damaging	1.00
IGL01407:Etl4	APN	2	20,748,667 (GRCm39)	missense	probably damaging	0.99
IGL01552:Etl4	APN	2	20,783,000 (GRCm39)	missense	probably damaging	0.99
IGL01662:Etl4	APN	2	20,811,460 (GRCm39)	missense	probably benign	0.04
IGL01687:Etl4	APN	2	20,534,898 (GRCm39)	missense	probably damaging	1.00
IGL01793:Etl4	APN	2	20,748,709 (GRCm39)	missense	possibly damaging	0.87
IGL01844:Etl4	APN	2	20,811,493 (GRCm39)	missense	probably benign	0.06
IGL02025:Etl4	APN	2	20,811,337 (GRCm39)	missense	probably damaging	1.00
IGL02088:Etl4	APN	2	20,811,359 (GRCm39)	missense	probably damaging	1.00
IGL02134:Etl4	APN	2	20,811,240 (GRCm39)	missense	possibly damaging	0.79
IGL02369:Etl4	APN	2	20,535,000 (GRCm39)	missense	probably damaging	1.00
IGL02480:Etl4	APN	2	20,793,335 (GRCm39)	missense	probably damaging	0.99
IGL02560:Etl4	APN	2	20,748,529 (GRCm39)	missense	probably damaging	1.00
IGL02851:Etl4	APN	2	20,812,840 (GRCm39)	missense	possibly damaging	0.46
IGL02893:Etl4	APN	2	20,765,021 (GRCm39)	splice site	probably benign
IGL02951:Etl4	APN	2	20,806,348 (GRCm39)	splice site	probably benign
IGL03119:Etl4	APN	2	20,718,198 (GRCm39)	missense	probably damaging	1.00
IGL03267:Etl4	APN	2	20,789,993 (GRCm39)	nonsense	probably null
IGL03379:Etl4	APN	2	20,666,827 (GRCm39)	missense	possibly damaging	0.87
R0038:Etl4	UTSW	2	20,748,385 (GRCm39)	missense	probably damaging	1.00
R0038:Etl4	UTSW	2	20,748,385 (GRCm39)	missense	probably damaging	1.00
R0095:Etl4	UTSW	2	20,748,679 (GRCm39)	missense	probably damaging	1.00
R0100:Etl4	UTSW	2	20,344,716 (GRCm39)	missense	probably benign
R0311:Etl4	UTSW	2	20,811,940 (GRCm39)	missense	probably damaging	1.00
R0346:Etl4	UTSW	2	20,764,463 (GRCm39)	critical splice donor site	probably null
R0348:Etl4	UTSW	2	20,782,940 (GRCm39)	missense	probably damaging	1.00
R0379:Etl4	UTSW	2	20,812,165 (GRCm39)	missense	probably damaging	0.98
R0571:Etl4	UTSW	2	20,748,580 (GRCm39)	missense	probably damaging	0.99
R0697:Etl4	UTSW	2	20,748,672 (GRCm39)	missense	probably damaging	1.00
R0707:Etl4	UTSW	2	20,810,382 (GRCm39)	splice site	probably benign
R0980:Etl4	UTSW	2	20,806,378 (GRCm39)	missense	probably damaging	1.00
R1120:Etl4	UTSW	2	20,811,514 (GRCm39)	missense	probably benign	0.00
R1254:Etl4	UTSW	2	20,812,734 (GRCm39)	missense	probably damaging	1.00
R1460:Etl4	UTSW	2	20,793,288 (GRCm39)	missense	probably damaging	1.00
R1503:Etl4	UTSW	2	20,748,685 (GRCm39)	missense	possibly damaging	0.94
R1547:Etl4	UTSW	2	20,790,039 (GRCm39)	missense	probably damaging	1.00
R1627:Etl4	UTSW	2	20,806,390 (GRCm39)	missense	possibly damaging	0.91
R1635:Etl4	UTSW	2	20,811,219 (GRCm39)	missense	probably damaging	1.00
R1716:Etl4	UTSW	2	20,748,492 (GRCm39)	missense	probably damaging	1.00
R1795:Etl4	UTSW	2	20,812,837 (GRCm39)	critical splice donor site	probably null
R1885:Etl4	UTSW	2	20,748,795 (GRCm39)	missense	probably damaging	1.00
R2039:Etl4	UTSW	2	20,790,039 (GRCm39)	missense	probably damaging	1.00
R2083:Etl4	UTSW	2	20,748,360 (GRCm39)	missense	probably damaging	1.00
R2109:Etl4	UTSW	2	20,790,153 (GRCm39)	missense	probably benign	0.27
R2153:Etl4	UTSW	2	20,803,545 (GRCm39)	missense	probably benign	0.00
R2403:Etl4	UTSW	2	20,812,117 (GRCm39)	nonsense	probably null
R2883:Etl4	UTSW	2	20,810,985 (GRCm39)	missense	possibly damaging	0.83
R2985:Etl4	UTSW	2	20,786,660 (GRCm39)	missense	probably damaging	1.00
R3402:Etl4	UTSW	2	20,786,693 (GRCm39)	missense	probably damaging	1.00
R3696:Etl4	UTSW	2	20,806,473 (GRCm39)	critical splice donor site	probably null
R3755:Etl4	UTSW	2	20,748,348 (GRCm39)	missense	probably benign	0.10
R3813:Etl4	UTSW	2	20,793,246 (GRCm39)	missense	probably damaging	1.00
R3829:Etl4	UTSW	2	20,790,232 (GRCm39)	missense	probably benign	0.07
R3887:Etl4	UTSW	2	20,534,772 (GRCm39)	nonsense	probably null
R3888:Etl4	UTSW	2	20,534,772 (GRCm39)	nonsense	probably null
R3889:Etl4	UTSW	2	20,534,772 (GRCm39)	nonsense	probably null
R3958:Etl4	UTSW	2	20,344,854 (GRCm39)	missense	probably benign
R3959:Etl4	UTSW	2	20,344,854 (GRCm39)	missense	probably benign
R3960:Etl4	UTSW	2	20,344,854 (GRCm39)	missense	probably benign
R4058:Etl4	UTSW	2	20,810,830 (GRCm39)	missense	possibly damaging	0.59
R4074:Etl4	UTSW	2	20,814,030 (GRCm39)	utr 3 prime	probably benign
R4077:Etl4	UTSW	2	20,812,772 (GRCm39)	missense	probably damaging	1.00
R4078:Etl4	UTSW	2	20,812,772 (GRCm39)	missense	probably damaging	1.00
R4127:Etl4	UTSW	2	20,748,886 (GRCm39)	missense	possibly damaging	0.93
R4200:Etl4	UTSW	2	20,786,694 (GRCm39)	missense	probably damaging	1.00
R4492:Etl4	UTSW	2	20,811,676 (GRCm39)	missense	possibly damaging	0.67
R4514:Etl4	UTSW	2	20,666,709 (GRCm39)	missense	probably damaging	1.00
R4820:Etl4	UTSW	2	20,811,496 (GRCm39)	missense	possibly damaging	0.85
R4825:Etl4	UTSW	2	20,811,738 (GRCm39)	missense	probably damaging	1.00
R4888:Etl4	UTSW	2	20,344,922 (GRCm39)	critical splice donor site	probably null
R4938:Etl4	UTSW	2	20,803,460 (GRCm39)	missense	probably benign	0.00
R4943:Etl4	UTSW	2	20,812,092 (GRCm39)	missense	probably benign	0.05
R5121:Etl4	UTSW	2	20,344,922 (GRCm39)	critical splice donor site	probably null
R5191:Etl4	UTSW	2	20,344,810 (GRCm39)	missense	probably damaging	0.99
R5198:Etl4	UTSW	2	20,718,198 (GRCm39)	missense	probably damaging	1.00
R5199:Etl4	UTSW	2	20,748,853 (GRCm39)	missense	probably damaging	1.00
R5470:Etl4	UTSW	2	20,534,791 (GRCm39)	missense	probably damaging	0.99
R5513:Etl4	UTSW	2	20,748,638 (GRCm39)	missense	probably damaging	1.00
R5620:Etl4	UTSW	2	20,535,037 (GRCm39)	missense	probably damaging	1.00
R5635:Etl4	UTSW	2	20,811,846 (GRCm39)	missense	probably damaging	1.00
R5641:Etl4	UTSW	2	20,811,273 (GRCm39)	frame shift	probably null
R5690:Etl4	UTSW	2	20,810,647 (GRCm39)	missense	probably benign	0.01
R5784:Etl4	UTSW	2	20,811,016 (GRCm39)	missense	possibly damaging	0.79
R5794:Etl4	UTSW	2	20,811,323 (GRCm39)	missense	probably damaging	1.00
R5908:Etl4	UTSW	2	20,748,718 (GRCm39)	missense	probably damaging	0.96
R5982:Etl4	UTSW	2	20,785,826 (GRCm39)	missense	probably damaging	1.00
R6151:Etl4	UTSW	2	20,718,171 (GRCm39)	missense	probably damaging	1.00
R6192:Etl4	UTSW	2	20,806,362 (GRCm39)	missense	probably damaging	0.98
R6238:Etl4	UTSW	2	20,806,379 (GRCm39)	missense	probably damaging	1.00
R6248:Etl4	UTSW	2	20,813,900 (GRCm39)	missense	possibly damaging	0.90
R6292:Etl4	UTSW	2	20,748,384 (GRCm39)	missense	probably damaging	1.00
R6610:Etl4	UTSW	2	20,718,180 (GRCm39)	missense	probably damaging	1.00
R6739:Etl4	UTSW	2	20,718,246 (GRCm39)	missense	probably damaging	1.00
R6846:Etl4	UTSW	2	20,748,919 (GRCm39)	missense	possibly damaging	0.94
R6863:Etl4	UTSW	2	20,811,120 (GRCm39)	missense	probably benign	0.01
R6873:Etl4	UTSW	2	20,802,803 (GRCm39)	splice site	probably null
R7003:Etl4	UTSW	2	20,810,695 (GRCm39)	missense	probably benign	0.03
R7155:Etl4	UTSW	2	20,811,742 (GRCm39)	missense	probably damaging	0.96
R7207:Etl4	UTSW	2	20,714,387 (GRCm39)	missense	probably damaging	0.99
R7230:Etl4	UTSW	2	20,802,799 (GRCm39)	missense	probably damaging	1.00
R7305:Etl4	UTSW	2	20,714,368 (GRCm39)	missense	probably damaging	1.00
R7389:Etl4	UTSW	2	20,789,904 (GRCm39)	nonsense	probably null
R7396:Etl4	UTSW	2	20,803,449 (GRCm39)	missense	possibly damaging	0.62
R7441:Etl4	UTSW	2	20,749,000 (GRCm39)	missense	possibly damaging	0.87
R7626:Etl4	UTSW	2	20,718,189 (GRCm39)	missense	probably damaging	1.00
R7776:Etl4	UTSW	2	20,811,957 (GRCm39)	missense	probably damaging	0.99
R7779:Etl4	UTSW	2	20,714,288 (GRCm39)	missense	probably damaging	1.00
R7798:Etl4	UTSW	2	20,786,757 (GRCm39)	critical splice donor site	probably null
R7851:Etl4	UTSW	2	20,748,951 (GRCm39)	missense	probably damaging	1.00
R7861:Etl4	UTSW	2	20,810,721 (GRCm39)	missense	probably benign
R7901:Etl4	UTSW	2	20,294,821 (GRCm39)	missense	possibly damaging	0.83
R8053:Etl4	UTSW	2	20,666,774 (GRCm39)	missense	probably damaging	1.00
R8124:Etl4	UTSW	2	20,811,451 (GRCm39)	missense	probably benign	0.06
R8133:Etl4	UTSW	2	20,811,082 (GRCm39)	missense	possibly damaging	0.86
R8203:Etl4	UTSW	2	20,789,916 (GRCm39)	missense	possibly damaging	0.61
R8238:Etl4	UTSW	2	20,811,342 (GRCm39)	nonsense	probably null
R8263:Etl4	UTSW	2	20,748,965 (GRCm39)	missense	probably benign	0.00
R8299:Etl4	UTSW	2	20,748,874 (GRCm39)	missense	possibly damaging	0.81
R8318:Etl4	UTSW	2	20,793,341 (GRCm39)	missense	probably damaging	1.00
R8334:Etl4	UTSW	2	20,785,857 (GRCm39)	missense	probably damaging	0.96
R8443:Etl4	UTSW	2	20,810,977 (GRCm39)	missense	probably benign	0.04
R8525:Etl4	UTSW	2	20,534,892 (GRCm39)	missense	probably damaging	1.00
R8679:Etl4	UTSW	2	20,714,288 (GRCm39)	missense	probably damaging	1.00
R8918:Etl4	UTSW	2	20,811,246 (GRCm39)	missense	probably benign	0.00
R8918:Etl4	UTSW	2	20,748,733 (GRCm39)	missense	probably benign
R9062:Etl4	UTSW	2	20,748,616 (GRCm39)	missense	probably damaging	0.99
R9095:Etl4	UTSW	2	20,782,964 (GRCm39)	missense	probably damaging	1.00
R9200:Etl4	UTSW	2	20,786,702 (GRCm39)	missense	probably damaging	1.00
R9416:Etl4	UTSW	2	20,748,784 (GRCm39)	missense	probably benign	0.17
R9437:Etl4	UTSW	2	20,813,872 (GRCm39)	missense	probably benign	0.20
R9451:Etl4	UTSW	2	20,813,926 (GRCm39)	missense	probably benign	0.03
R9489:Etl4	UTSW	2	20,771,345 (GRCm39)	missense	possibly damaging	0.75
R9531:Etl4	UTSW	2	20,294,818 (GRCm39)	start codon destroyed	probably null	0.01
R9605:Etl4	UTSW	2	20,771,345 (GRCm39)	missense	possibly damaging	0.75
R9623:Etl4	UTSW	2	20,811,052 (GRCm39)	missense
R9631:Etl4	UTSW	2	20,666,749 (GRCm39)	missense	probably benign	0.28
R9632:Etl4	UTSW	2	20,666,749 (GRCm39)	missense	probably benign	0.28
R9646:Etl4	UTSW	2	20,802,724 (GRCm39)	missense	probably benign	0.00
R9732:Etl4	UTSW	2	20,748,373 (GRCm39)	missense	probably damaging	0.98
R9755:Etl4	UTSW	2	20,790,048 (GRCm39)	missense	probably benign	0.17
R9771:Etl4	UTSW	2	20,811,537 (GRCm39)	missense	probably benign
RF003:Etl4	UTSW	2	20,524,729 (GRCm39)	nonsense	probably null
X0018:Etl4	UTSW	2	20,814,001 (GRCm39)	missense	probably damaging	0.98
X0022:Etl4	UTSW	2	20,714,375 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TGACACCTTCACAGAGGAAAATGCC -3'
(R):5'- TCCTCACTGGGAGAACTTGGAGTTG -3'

Sequencing Primer
(F):5'- TTCCCAGAAAAATGTGCCTAAGG -3'
(R):5'- GATTCTCCATCCTCATTCATGTTC -3'

Posted On

2014-02-11