Mutagenetix > Incidental Mutation

Incidental Mutation 'R1349:Vmn2r63'

156599

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r63

Ensembl Gene

ENSMUSG00000090751

Gene Name

vomeronasal 2, receptor 63

Synonyms

EG435975

MMRRC Submission

039414-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.074) question?

Stock #

R1349 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

42552675-42583213 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 42578642 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Phenylalanine to Leucine at position 84 (F84L)

Ref Sequence

ENSEMBL: ENSMUSP00000129089 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000163803]

AlphaFold

E9Q0K5

Predicted Effect

possibly damaging
Transcript: ENSMUST00000163803
AA Change: F84L

PolyPhen 2 Score 0.955 (Sensitivity: 0.79; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000129089
Gene: ENSMUSG00000090751
AA Change: F84L

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	77	471	3.5e-43	PFAM
Pfam:NCD3G	514	567	5.1e-23	PFAM
Pfam:7tm_3	600	835	3.5e-51	PFAM

Meta Mutation Damage Score

0.1795

Coding Region Coverage

1x: 99.0%
3x: 98.1%
10x: 95.6%
20x: 90.8%

Validation Efficiency

98% (46/47)

Allele List at MGI

Other mutations in this stock

Total: 45 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2810004N23Rik	G	A	8: 125,587,992 (GRCm39)	T36I	possibly damaging	Het
Adcy2	T	A	13: 68,816,652 (GRCm39)	N778I	probably damaging	Het
Ak5	G	T	3: 152,239,071 (GRCm39)	D301E	probably damaging	Het
Akap13	G	A	7: 75,259,340 (GRCm39)	G655S	possibly damaging	Het
Ankrd28	A	T	14: 31,467,218 (GRCm39)	M248K	probably benign	Het
Atf7ip2	G	A	16: 10,052,195 (GRCm39)	V225I	probably damaging	Het
Ccdc157	A	T	11: 4,099,056 (GRCm39)	I48N	probably benign	Het
Cd209d	C	A	8: 3,928,515 (GRCm39)		probably benign	Het
Cecr2	G	A	6: 120,734,564 (GRCm39)	G613E	probably damaging	Het
Clspn	C	T	4: 126,457,770 (GRCm39)	A98V	probably benign	Het
Cntnap5b	G	A	1: 100,091,813 (GRCm39)	D499N	probably benign	Het
Cox7a2	G	A	9: 79,665,819 (GRCm39)	R21*	probably null	Het
Cul9	C	G	17: 46,833,101 (GRCm39)	A1326P	probably damaging	Het
Dbpht2	C	CNNNNNNNNNNNNNNNNNN	12: 74,345,836 (GRCm39)		noncoding transcript	Het
Dlg1	T	C	16: 31,631,638 (GRCm39)	I208T	probably damaging	Het
Dmxl1	A	T	18: 50,021,920 (GRCm39)	N1612Y	probably damaging	Het
Epha3	A	G	16: 63,431,416 (GRCm39)	I495T	possibly damaging	Het
Frem1	T	C	4: 82,840,542 (GRCm39)		probably benign	Het
Glipr1	A	G	10: 111,829,437 (GRCm39)	V108A	probably benign	Het
Gpatch2l	T	C	12: 86,307,483 (GRCm39)	L287P	possibly damaging	Het
Hp	T	G	8: 110,301,938 (GRCm39)	K337Q	probably benign	Het
Htr1a	T	A	13: 105,581,874 (GRCm39)	C371*	probably null	Het
Leo1	T	C	9: 75,356,751 (GRCm39)	V377A	possibly damaging	Het
Lsg1	A	G	16: 30,383,472 (GRCm39)	F583L	possibly damaging	Het
Map4k4	C	A	1: 40,060,319 (GRCm39)	P1103Q	probably damaging	Het
Mybph	T	C	1: 134,121,353 (GRCm39)	S38P	probably benign	Het
Myo1e	T	G	9: 70,194,351 (GRCm39)		probably benign	Het
Nefh	T	TNNNNNNNNNNNNNNNNNN	11: 4,891,010 (GRCm39)		probably benign	Het
Oca2	T	A	7: 56,185,716 (GRCm39)	M814K	probably benign	Het
Odad3	C	T	9: 21,904,916 (GRCm39)	R290H	probably damaging	Het
Pkd1	T	C	17: 24,794,240 (GRCm39)	C1976R	probably damaging	Het
Pogz	T	A	3: 94,768,199 (GRCm39)	L126M	probably damaging	Het
Rec8	T	C	14: 55,856,431 (GRCm39)	Y68H	probably damaging	Het
Ryr3	T	A	2: 112,664,546 (GRCm39)	S1582C	probably damaging	Het
Sh3pxd2a	A	T	19: 47,256,160 (GRCm39)	W853R	probably damaging	Het
Slc6a7	C	T	18: 61,133,615 (GRCm39)	G527D	probably benign	Het
Spopfm1	A	G	3: 94,173,435 (GRCm39)	T148A	possibly damaging	Het
Tgm1	A	G	14: 55,948,658 (GRCm39)		probably benign	Het
Tnxb	T	C	17: 34,929,267 (GRCm39)	V2770A	possibly damaging	Het
Togaram1	T	A	12: 65,057,919 (GRCm39)	M1502K	probably damaging	Het
Vmn1r11	G	A	6: 57,114,963 (GRCm39)	C209Y	probably benign	Het
Vmn2r102	A	T	17: 19,880,887 (GRCm39)		probably benign	Het
Vmn2r12	T	C	5: 109,234,452 (GRCm39)	M587V	probably benign	Het
Wdr35	A	T	12: 9,069,870 (GRCm39)		probably benign	Het
Wdr73	C	A	7: 80,543,000 (GRCm39)	V176L	probably damaging	Het

Other mutations in Vmn2r63

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01399:Vmn2r63	APN	7	42,553,543 (GRCm39)	missense	probably damaging	0.99
IGL01765:Vmn2r63	APN	7	42,552,788 (GRCm39)	missense	probably benign
IGL02203:Vmn2r63	APN	7	42,553,432 (GRCm39)	missense	probably benign	0.01
IGL02825:Vmn2r63	APN	7	42,576,274 (GRCm39)	critical splice donor site	probably null
IGL03155:Vmn2r63	APN	7	42,552,878 (GRCm39)	missense	probably damaging	1.00
IGL03260:Vmn2r63	APN	7	42,578,616 (GRCm39)	missense	probably damaging	1.00
IGL03411:Vmn2r63	APN	7	42,577,368 (GRCm39)	missense	probably benign	0.42
PIT4280001:Vmn2r63	UTSW	7	42,553,409 (GRCm39)	missense	probably damaging	1.00
R0066:Vmn2r63	UTSW	7	42,576,514 (GRCm39)	splice site	probably benign
R0328:Vmn2r63	UTSW	7	42,552,699 (GRCm39)	missense	probably benign	0.09
R0344:Vmn2r63	UTSW	7	42,553,042 (GRCm39)	missense	probably damaging	0.98
R0554:Vmn2r63	UTSW	7	42,583,129 (GRCm39)	nonsense	probably null
R0555:Vmn2r63	UTSW	7	42,577,952 (GRCm39)	nonsense	probably null
R0685:Vmn2r63	UTSW	7	42,577,434 (GRCm39)	missense	probably benign	0.13
R0751:Vmn2r63	UTSW	7	42,577,459 (GRCm39)	missense	probably damaging	1.00
R1372:Vmn2r63	UTSW	7	42,578,642 (GRCm39)	missense	possibly damaging	0.95
R1416:Vmn2r63	UTSW	7	42,577,339 (GRCm39)	missense	probably benign	0.00
R1502:Vmn2r63	UTSW	7	42,578,015 (GRCm39)	missense	possibly damaging	0.62
R1563:Vmn2r63	UTSW	7	42,553,550 (GRCm39)	missense	probably benign	0.00
R1652:Vmn2r63	UTSW	7	42,577,635 (GRCm39)	missense	probably benign	0.32
R1693:Vmn2r63	UTSW	7	42,577,743 (GRCm39)	missense	probably benign
R1698:Vmn2r63	UTSW	7	42,583,038 (GRCm39)	missense	probably benign
R1753:Vmn2r63	UTSW	7	42,577,669 (GRCm39)	nonsense	probably null
R2136:Vmn2r63	UTSW	7	42,576,297 (GRCm39)	missense	probably damaging	0.99
R2175:Vmn2r63	UTSW	7	42,583,004 (GRCm39)	critical splice donor site	probably null
R2261:Vmn2r63	UTSW	7	42,578,031 (GRCm39)	missense	probably benign	0.02
R2262:Vmn2r63	UTSW	7	42,578,031 (GRCm39)	missense	probably benign	0.02
R2263:Vmn2r63	UTSW	7	42,578,031 (GRCm39)	missense	probably benign	0.02
R3413:Vmn2r63	UTSW	7	42,576,406 (GRCm39)	missense	probably benign	0.04
R3426:Vmn2r63	UTSW	7	42,576,406 (GRCm39)	missense	probably benign	0.04
R3427:Vmn2r63	UTSW	7	42,576,406 (GRCm39)	missense	probably benign	0.04
R3802:Vmn2r63	UTSW	7	42,552,829 (GRCm39)	missense	probably damaging	0.99
R4319:Vmn2r63	UTSW	7	42,576,406 (GRCm39)	missense	probably benign	0.04
R4321:Vmn2r63	UTSW	7	42,576,406 (GRCm39)	missense	probably benign	0.04
R4323:Vmn2r63	UTSW	7	42,576,406 (GRCm39)	missense	probably benign	0.04
R4346:Vmn2r63	UTSW	7	42,577,537 (GRCm39)	missense	possibly damaging	0.67
R4568:Vmn2r63	UTSW	7	42,583,250 (GRCm39)	splice site	probably null
R4649:Vmn2r63	UTSW	7	42,553,114 (GRCm39)	missense	possibly damaging	0.79
R4653:Vmn2r63	UTSW	7	42,553,114 (GRCm39)	missense	possibly damaging	0.79
R4679:Vmn2r63	UTSW	7	42,577,544 (GRCm39)	missense	probably benign	0.00
R4734:Vmn2r63	UTSW	7	42,577,544 (GRCm39)	missense	probably benign	0.00
R4741:Vmn2r63	UTSW	7	42,577,544 (GRCm39)	missense	probably benign	0.00
R4748:Vmn2r63	UTSW	7	42,577,544 (GRCm39)	missense	probably benign	0.00
R4806:Vmn2r63	UTSW	7	42,576,314 (GRCm39)	missense	probably benign	0.34
R4933:Vmn2r63	UTSW	7	42,553,402 (GRCm39)	missense	probably damaging	1.00
R5198:Vmn2r63	UTSW	7	42,553,169 (GRCm39)	missense	probably benign	0.01
R5399:Vmn2r63	UTSW	7	42,577,701 (GRCm39)	missense	probably benign
R5400:Vmn2r63	UTSW	7	42,577,635 (GRCm39)	missense	probably benign	0.32
R6158:Vmn2r63	UTSW	7	42,583,104 (GRCm39)	missense	probably damaging	1.00
R6185:Vmn2r63	UTSW	7	42,578,435 (GRCm39)	missense	probably damaging	0.98
R6267:Vmn2r63	UTSW	7	42,578,059 (GRCm39)	splice site	probably null
R6362:Vmn2r63	UTSW	7	42,552,721 (GRCm39)	missense	probably benign
R6706:Vmn2r63	UTSW	7	42,578,001 (GRCm39)	missense	probably damaging	1.00
R6764:Vmn2r63	UTSW	7	42,552,695 (GRCm39)	missense	probably damaging	0.97
R7104:Vmn2r63	UTSW	7	42,577,959 (GRCm39)	missense	possibly damaging	0.67
R7503:Vmn2r63	UTSW	7	42,583,014 (GRCm39)	missense	probably benign	0.02
R7506:Vmn2r63	UTSW	7	42,576,391 (GRCm39)	missense	probably damaging	1.00
R7525:Vmn2r63	UTSW	7	42,576,406 (GRCm39)	missense	possibly damaging	0.52
R7658:Vmn2r63	UTSW	7	42,574,693 (GRCm39)	missense	probably damaging	1.00
R7663:Vmn2r63	UTSW	7	42,576,466 (GRCm39)	missense	probably benign	0.00
R7702:Vmn2r63	UTSW	7	42,577,553 (GRCm39)	missense	possibly damaging	0.46
R7918:Vmn2r63	UTSW	7	42,552,955 (GRCm39)	missense	probably damaging	1.00
R8826:Vmn2r63	UTSW	7	42,576,441 (GRCm39)	missense	probably benign	0.38
R8973:Vmn2r63	UTSW	7	42,577,919 (GRCm39)	missense	probably benign	0.19
R9024:Vmn2r63	UTSW	7	42,577,874 (GRCm39)	missense	probably benign	0.14
R9154:Vmn2r63	UTSW	7	42,576,413 (GRCm39)	missense	probably damaging	0.96
R9731:Vmn2r63	UTSW	7	42,553,361 (GRCm39)	missense	probably benign	0.32
Z1088:Vmn2r63	UTSW	7	42,577,983 (GRCm39)	missense	probably benign	0.09

Predicted Primers

PCR Primer
(F):5'- ATGACGTTCCAGTGAGTGCTGC -3'
(R):5'- GTCACAGAAACTTGGGTGACTTGGT -3'

Sequencing Primer
(F):5'- TGCAGGTAAATTTCTTTTCTGGC -3'
(R):5'- CTGTAGAGACTGATAAGCCAAGATTC -3'

Posted On

2014-02-11