|Institutional Source||Beutler Lab|
|Gene Name||NIMA (never in mitosis gene a)-related expressed kinase 3|
|Is this an essential gene?||Non essential (E-score: 0.000)|
|Stock #||R0001 (G1)|
|Chromosomal Location||22128283-22166435 bp(-) (GRCm38)|
|Type of Mutation||splice site|
|DNA Base Change (assembly)||A to T at 22158612 bp (GRCm38)|
|Amino Acid Change|
|Ref Sequence||ENSEMBL: ENSMUSP00000136876 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000033865] [ENSMUST00000110730] [ENSMUST00000178324]|
|Meta Mutation Damage Score||0.0898|
|Coding Region Coverage||
|Validation Efficiency||99% (76/77)|
|MGI Phenotype||FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the NimA (never in mitosis A) family of serine/threonine protein kinases. The encoded protein differs from other NimA family members in that it is not cell cycle regulated and is found primarily in the cytoplasm. The kinase is activated by prolactin stimulation, leading to phosphorylation of VAV2 guanine nucleotide exchange factor, paxillin, and activation of the RAC1 GTPase. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2009]|
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Nek3||
(F):5'- CCTAAAAGAAGGGAAggggctagagac -3'
(R):5'- ACAAAACATGGGAAACGGATTTCTGAAC -3'
(F):5'- cacacacacacacacacac -3'
(R):5'- GGAAACGGATTTCTGAACTTCTCC -3'