Mutagenetix > Incidental Mutations

Incidental Mutation 'R1325:Cry2'

157177

Institutional Source

Beutler Lab

Gene Symbol

Cry2

Ensembl Gene

ENSMUSG00000068742

Gene Name

cryptochrome 2 (photolyase-like)

Synonyms

MMRRC Submission

039391-MU

Accession Numbers

Is this an essential gene?

Probably essential (E-score: 0.752) question?

Stock #

R1325 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

92403646-92434043 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 92413770 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 353 (T353A)

Ref Sequence

ENSEMBL: ENSMUSP00000106909 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000090559] [ENSMUST00000111278]

AlphaFold

Q9R194

Predicted Effect

probably damaging
Transcript: ENSMUST00000090559
AA Change: T353A

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000088047
Gene: ENSMUSG00000068742
AA Change: T353A

Domain	Start	End	E-Value	Type
low complexity region	2	13	N/A	INTRINSIC
Pfam:DNA_photolyase	23	187	2.4e-50	PFAM
Pfam:FAD_binding_7	231	504	4.4e-89	PFAM
low complexity region	562	573	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000111278
AA Change: T353A

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000106909
Gene: ENSMUSG00000068742
AA Change: T353A

Domain	Start	End	E-Value	Type
low complexity region	2	13	N/A	INTRINSIC
Pfam:DNA_photolyase	23	189	3.6e-50	PFAM
Pfam:FAD_binding_7	230	506	1.4e-105	PFAM
low complexity region	562	573	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000126002

Coding Region Coverage

1x: 98.9%
3x: 98.1%
10x: 95.8%
20x: 91.5%

Validation Efficiency

MGI Phenotype

FUNCTION: This gene encodes a flavin adenine dinucleotide-binding protein that is a key component of the circadian core oscillator complex, which regulates the circadian clock. This gene is upregulated by Clock/Arntl heterodimers but then represses this upregulation in a feedback loop using Per/Cry heterodimers to interact with Clock/Arntl. Polymorphisms in this gene have been associated with altered sleep patterns. The encoded protein is widely conserved across plants and animals. [provided by RefSeq, Feb 2014]
PHENOTYPE: Homozygotes for targeted null mutations exhibit a one-hour longer circadian period under constant darkness, and reduced expression of another circadian gene in the suprachiasmatic nucleus in response to acute light exposure. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 21 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4921504E06Rik	A	G	2: 19,495,127	I293T	possibly damaging	Het
Abca14	A	G	7: 120,247,322	I666V	probably benign	Het
Acsl1	G	A	8: 46,513,300	V164I	probably benign	Het
Aox3	C	T	1: 58,176,567	Q1053*	probably null	Het
Arhgap31	A	G	16: 38,602,942	S921P	probably benign	Het
Bcl6	A	G	16: 23,972,347	M419T	probably benign	Het
Bdp1	A	G	13: 100,099,008	I145T	probably damaging	Het
Btaf1	T	C	19: 36,969,162	V456A	possibly damaging	Het
Dcxr	A	G	11: 120,726,555		probably null	Het
Echdc1	A	T	10: 29,317,548	T14S	probably benign	Het
Fgd6	G	A	10: 94,127,427	R1129H	probably damaging	Het
Fstl1	A	G	16: 37,828,721	D197G	probably damaging	Het
Gjb3	G	A	4: 127,326,431	R103W	probably damaging	Het
Krt1	A	T	15: 101,848,206		probably null	Het
Lrrc9	C	A	12: 72,497,104	Q1116K	probably damaging	Het
Ncor2	T	C	5: 125,118,780		probably benign	Het
Nrbp1	T	A	5: 31,245,813	I210N	probably damaging	Het
Olfr531	G	A	7: 140,400,881	P55L	probably damaging	Het
Prl2c2	G	C	13: 13,002,201	T47R	probably damaging	Het
Slc17a6	A	G	7: 51,661,552	E338G	probably benign	Het
Ttn	A	G	2: 76,900,394		probably benign	Het

Other mutations in Cry2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01995:Cry2	APN	2	92424632	missense	probably benign	0.15
IGL02167:Cry2	APN	2	92433821	missense	possibly damaging	0.93
IGL02183:Cry2	APN	2	92413039	missense	probably damaging	0.99
IGL02343:Cry2	APN	2	92426921	missense	possibly damaging	0.90
IGL02432:Cry2	APN	2	92413667	missense	probably damaging	0.99
IGL02725:Cry2	APN	2	92413260	splice site	probably benign
IGL02932:Cry2	APN	2	92413117	nonsense	probably null
IGL03122:Cry2	APN	2	92413295	missense	probably damaging	1.00
IGL03366:Cry2	APN	2	92413715	missense	probably damaging	1.00
R0679:Cry2	UTSW	2	92413715	missense	probably damaging	1.00
R1862:Cry2	UTSW	2	92424566	missense	probably damaging	1.00
R1891:Cry2	UTSW	2	92413640	missense	possibly damaging	0.93
R2189:Cry2	UTSW	2	92411692	missense	possibly damaging	0.84
R4032:Cry2	UTSW	2	92413827	missense	probably benign	0.00
R4689:Cry2	UTSW	2	92424554	missense	probably benign	0.38
R5130:Cry2	UTSW	2	92424599	missense	probably benign	0.28
R5145:Cry2	UTSW	2	92413060	missense	probably benign
R5970:Cry2	UTSW	2	92412967	missense	probably benign	0.08
R6179:Cry2	UTSW	2	92413842	missense	probably damaging	0.98
R7102:Cry2	UTSW	2	92413093	missense	probably damaging	0.99
R7158:Cry2	UTSW	2	92413715	missense	probably damaging	1.00
R7213:Cry2	UTSW	2	92413659	missense	probably benign	0.00
R7257:Cry2	UTSW	2	92412981	missense	possibly damaging	0.67
R7378:Cry2	UTSW	2	92413664	missense	probably damaging	1.00
R7427:Cry2	UTSW	2	92413047	missense	possibly damaging	0.74
R7428:Cry2	UTSW	2	92413047	missense	possibly damaging	0.74
R7440:Cry2	UTSW	2	92413638	missense	probably damaging	1.00
R7531:Cry2	UTSW	2	92413005	missense	probably damaging	0.98
R8234:Cry2	UTSW	2	92412629	missense	probably benign
R8350:Cry2	UTSW	2	92413941	missense	probably benign	0.00
R8450:Cry2	UTSW	2	92413941	missense	probably benign	0.00
R8496:Cry2	UTSW	2	92426939	missense	probably damaging	1.00
R9172:Cry2	UTSW	2	92413648	missense	probably damaging	1.00
R9283:Cry2	UTSW	2	92413904	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- ACTATTACACCCTCAGGGAGCCAG -3'
(R):5'- AGCATCGTCACAGTGCTAAGAGCC -3'

Sequencing Primer
(F):5'- CCCAGTCTATAAGGAGCTGC -3'
(R):5'- AGGCAGACACTCATCTCTGTG -3'

Posted On

2014-02-18