Mutagenetix > Incidental Mutations

Incidental Mutation 'R1319:Ang2'

157643

Institutional Source

Beutler Lab

Gene Symbol

Ang2

Ensembl Gene

ENSMUSG00000047894

Gene Name

angiogenin, ribonuclease A family, member 2

Synonyms

Rnase5b, Angrp

MMRRC Submission

039385-MU

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.062) question?

Stock #

R1319 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

51195324-51199148 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 51195707 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 73 (T73A)

Ref Sequence

ENSEMBL: ENSMUSP00000154100 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000051274] [ENSMUST00000228835]

AlphaFold

Q64438

Predicted Effect

probably benign
Transcript: ENSMUST00000051274
AA Change: T73A

PolyPhen 2 Score 0.024 (Sensitivity: 0.95; Specificity: 0.81)

SMART Domains

Protein: ENSMUSP00000062902
Gene: ENSMUSG00000047894
AA Change: T73A

Domain	Start	End	E-Value	Type
low complexity region	5	18	N/A	INTRINSIC
RNAse_Pc	26	142	3.11e-62	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000228835
AA Change: T73A

PolyPhen 2 Score 0.024 (Sensitivity: 0.95; Specificity: 0.81)

Coding Region Coverage

1x: 99.0%
3x: 98.1%
10x: 95.5%
20x: 90.3%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 26 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700021F07Rik	A	T	2: 173,527,923	S77C	probably damaging	Het
Adam28	T	C	14: 68,609,129	E745G	probably benign	Het
Adamts12	G	T	15: 11,286,791	K827N	probably benign	Het
Bbs10	T	C	10: 111,298,874	L51P	probably damaging	Het
Bean1	T	C	8: 104,217,224	I137T	probably benign	Het
Cttnbp2	T	C	6: 18,434,630	T410A	probably benign	Het
Cyp4a10	A	C	4: 115,521,145	I143L	probably damaging	Het
Dlg2	A	T	7: 92,438,023	Q788L	probably damaging	Het
Epha10	G	A	4: 124,881,914	V14I	probably benign	Het
Eprs	G	A	1: 185,384,962	D401N	probably damaging	Het
Fam169a	T	C	13: 97,097,562	V114A	probably damaging	Het
Fbn2	G	A	18: 58,200,610	P178S	possibly damaging	Het
Fcrls	A	C	3: 87,262,177		probably null	Het
Grm1	G	T	10: 10,689,398	H1055Q	probably benign	Het
Mcm6	T	C	1: 128,349,052	N267S	probably benign	Het
Olfr262	T	C	19: 12,241,502	D53G	probably damaging	Het
Phc3	T	C	3: 30,929,869	I699V	probably damaging	Het
Prl2c2	G	C	13: 13,002,201	T47R	probably damaging	Het
Pyroxd1	T	C	6: 142,359,148	V367A	probably benign	Het
R3hdm1	G	A	1: 128,231,405	R939H	probably benign	Het
Rag1	A	T	2: 101,643,192	I535N	probably damaging	Het
Rhot1	T	C	11: 80,246,021	C310R	probably damaging	Het
Tnrc6a	G	A	7: 123,184,251	V1481M	probably benign	Het
Vmn1r234	A	T	17: 21,228,910	M29L	probably benign	Het
Vmn2r68	A	G	7: 85,232,492	I460T	probably damaging	Het
Zfhx3	T	A	8: 108,933,833	Y1240N	probably damaging	Het

Other mutations in Ang2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R0276:Ang2	UTSW	14	51195518	missense	probably damaging	0.98
R1548:Ang2	UTSW	14	51195533	nonsense	probably null
R3736:Ang2	UTSW	14	51195656	nonsense	probably null
R4329:Ang2	UTSW	14	51195868	missense	possibly damaging	0.77
R4754:Ang2	UTSW	14	51195517	missense	probably damaging	1.00
R5457:Ang2	UTSW	14	51195835	missense	probably damaging	1.00
R7718:Ang2	UTSW	14	51195760	missense	probably benign
R8317:Ang2	UTSW	14	51195892	missense	probably benign	0.34
R8325:Ang2	UTSW	14	51195503	missense	probably damaging	1.00
R8725:Ang2	UTSW	14	51195539	missense	probably benign	0.40
R8727:Ang2	UTSW	14	51195539	missense	probably benign	0.40

Predicted Primers

PCR Primer
(F):5'- AAAGTGGACAGGCCAGCCATTCTC -3'
(R):5'- CCTTGGCAGGAATCTCTGTTGGAAG -3'

Sequencing Primer
(F):5'- GCCAGCCATTCTCACAGC -3'
(R):5'- GATGAGCCCAGGTCCTTTG -3'

Posted On

2014-02-18