Mutagenetix > Incidental Mutation

Incidental Mutation 'R1305:Ifna2'

157769

Institutional Source

Beutler Lab

Gene Symbol

Ifna2

Ensembl Gene

ENSMUSG00000078354

Gene Name

interferon alpha 2

Synonyms

Ifa2

MMRRC Submission

039371-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.065) question?

Stock #

R1305 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

88601444-88602016 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 88601614 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 135 (T135A)

Ref Sequence

ENSEMBL: ENSMUSP00000100778 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000105147]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000105147
AA Change: T135A

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000100778
Gene: ENSMUSG00000078354
AA Change: T135A

Domain	Start	End	E-Value	Type
signal peptide	1	23	N/A	INTRINSIC
IFabd	58	175	1.24e-70	SMART

Coding Region Coverage

1x: 98.9%
3x: 98.2%
10x: 96.1%
20x: 91.9%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 25 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Atxn2	G	A	5: 121,887,247 (GRCm39)	V306I	probably damaging	Het
Enc1	T	C	13: 97,383,208 (GRCm39)	Y573H	possibly damaging	Het
Gm9869	T	C	9: 60,729,168 (GRCm39)		probably benign	Het
Ifngr1	C	T	10: 19,482,001 (GRCm39)	T197M	possibly damaging	Het
Kcnj4	A	T	15: 79,369,020 (GRCm39)	V320E	probably damaging	Het
Map2	T	A	1: 66,464,554 (GRCm39)	I1648N	probably damaging	Het
Map4k1	A	T	7: 28,694,890 (GRCm39)	M435L	probably benign	Het
Mark3	T	A	12: 111,581,880 (GRCm39)		probably null	Het
Mex3c	T	A	18: 73,723,306 (GRCm39)	F466L	probably benign	Het
Msl3l2	T	C	10: 55,991,631 (GRCm39)	C119R	probably damaging	Het
Msln	T	C	17: 25,972,001 (GRCm39)	E72G	probably benign	Het
Nid2	T	C	14: 19,818,930 (GRCm39)	S475P	probably benign	Het
Nme8	T	A	13: 19,881,077 (GRCm39)	N18I	possibly damaging	Het
Nwd2	G	T	5: 63,902,540 (GRCm39)	W86L	probably damaging	Het
Or4p21	A	T	2: 88,276,646 (GRCm39)	L212*	probably null	Het
Or7a37	T	C	10: 78,805,933 (GRCm39)	I150T	probably benign	Het
Pard3	T	A	8: 128,032,891 (GRCm39)	S162T	possibly damaging	Het
Ppm1a	C	A	12: 72,830,494 (GRCm39)	D6E	probably damaging	Het
Rcor3	C	T	1: 191,800,646 (GRCm39)	V312I	possibly damaging	Het
Rgs20	T	A	1: 5,091,262 (GRCm39)		probably null	Het
Slc11a2	C	A	15: 100,307,963 (GRCm39)		probably null	Het
Snrnp40	C	G	4: 130,271,836 (GRCm39)		probably null	Het
Vmn1r124	C	A	7: 20,994,188 (GRCm39)	V119F	probably benign	Het
Zfp827	A	G	8: 79,787,523 (GRCm39)	T230A	possibly damaging	Het
Zfp945	T	C	17: 23,071,360 (GRCm39)	K180E	probably damaging	Het

Other mutations in Ifna2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01882:Ifna2	APN	4	88,601,979 (GRCm39)	missense	possibly damaging	0.87
IGL03247:Ifna2	APN	4	88,601,614 (GRCm39)	missense	probably benign	0.00
PIT4687001:Ifna2	UTSW	4	88,601,542 (GRCm39)	missense	possibly damaging	0.92
R0691:Ifna2	UTSW	4	88,601,895 (GRCm39)	missense	probably damaging	1.00
R6470:Ifna2	UTSW	4	88,601,751 (GRCm39)	missense	probably benign	0.01
R7886:Ifna2	UTSW	4	88,601,506 (GRCm39)	missense	probably damaging	1.00
R8560:Ifna2	UTSW	4	88,601,502 (GRCm39)	missense	possibly damaging	0.93
R8931:Ifna2	UTSW	4	88,601,712 (GRCm39)	missense	probably benign	0.30
R9037:Ifna2	UTSW	4	88,601,664 (GRCm39)	missense	probably benign	0.11
X0013:Ifna2	UTSW	4	88,601,771 (GRCm39)	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- AGATGCAGTTTCTAGTCCAGGGGAG -3'
(R):5'- GATAACCAGCAGATCCAGAAGGCTC -3'

Sequencing Primer
(F):5'- CACACTTTGTCTCACACTCAC -3'
(R):5'- GAAGGCTCAAGCCATCCCTG -3'

Posted On

2014-02-18