Mutagenetix > Incidental Mutation

Incidental Mutation 'R1296:Mslnl'

158155

Institutional Source

Beutler Lab

Gene Symbol

Mslnl

Ensembl Gene

ENSMUSG00000041062

Gene Name

mesothelin-like

Synonyms

MMRRC Submission

039362-MU

Accession Numbers

Is this an essential gene?

Non essential (E-score: 0.000) question?

Stock #

R1296 (G1)

Quality Score

122

Status

Validated

Chromosome

Chromosomal Location

25736040-25748330 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 25743240 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Leucine to Proline at position 204 (L204P)

Ref Sequence

ENSEMBL: ENSMUSP00000049020 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000047098]

AlphaFold

Q8C160

Predicted Effect

probably damaging
Transcript: ENSMUST00000047098
AA Change: L204P

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000049020
Gene: ENSMUSG00000041062
AA Change: L204P

Domain	Start	End	E-Value	Type
signal peptide	1	23	N/A	INTRINSIC
Pfam:Mesothelin	29	589	2.8e-70	PFAM
low complexity region	633	653	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000102319

Meta Mutation Damage Score

0.5693

Coding Region Coverage

1x: 98.8%
3x: 97.7%
10x: 94.5%
20x: 87.1%

Validation Efficiency

100% (72/72)

Allele List at MGI

Other mutations in this stock

Total: 63 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adam29	G	A	8: 55,871,719	Q567*	probably null	Het
Apol11a	T	C	15: 77,511,019		probably benign	Het
Arhgap29	A	G	3: 121,992,395	H275R	probably benign	Het
Arhgef17	C	A	7: 100,881,269	E428*	probably null	Het
Atm	A	T	9: 53,456,530	V2431E	probably damaging	Het
Atn1	G	T	6: 124,747,787	P161Q	unknown	Het
Atp13a2	T	C	4: 140,993,802	S99P	probably damaging	Het
Atp8a1	A	T	5: 67,622,706		probably benign	Het
Cbwd1	A	T	19: 24,942,675		probably benign	Het
Cdk18	T	C	1: 132,119,960		probably benign	Het
Cep85	A	G	4: 134,167,400	W32R	probably damaging	Het
Cntn4	G	T	6: 106,509,402	G264C	probably damaging	Het
Col6a4	A	G	9: 106,062,853	S1293P	possibly damaging	Het
Col6a6	T	C	9: 105,781,091	K641E	probably damaging	Het
Dmd	A	T	X: 83,878,520	K1465N	probably damaging	Het
Dus2	T	C	8: 106,053,043	V403A	possibly damaging	Het
Frs2	C	T	10: 117,081,074	C5Y	probably benign	Het
Gm5174	A	G	10: 86,657,002		noncoding transcript	Het
Gm6990	T	A	19: 56,755,334		noncoding transcript	Het
Gpr61	A	G	3: 108,150,481	V288A	possibly damaging	Het
Grik3	G	A	4: 125,704,564		probably benign	Het
Haao	T	A	17: 83,838,838	Q69L	probably benign	Het
Ints6	T	C	14: 62,704,903		probably benign	Het
Ints8	T	C	4: 11,221,204	I724V	possibly damaging	Het
Lrrk2	G	A	15: 91,728,920	C749Y	probably damaging	Het
Map4k1	A	G	7: 28,998,452	D471G	possibly damaging	Het
Mbtd1	A	G	11: 93,910,359	Y122C	probably damaging	Het
Mrfap1	A	G	5: 36,796,473	S41P	possibly damaging	Het
Mrm2	T	C	5: 140,328,553	T176A	probably benign	Het
Muc6	T	C	7: 141,651,879	E112G	probably benign	Het
Nfyb	A	G	10: 82,750,831		probably benign	Het
Nlgn3	T	C	X: 101,308,916		probably benign	Het
Nr3c1	G	A	18: 39,486,998	Q79*	probably null	Het
Nxpe4	C	G	9: 48,396,493	T299R	probably benign	Het
Otud4	C	A	8: 79,673,974	H1105N	unknown	Het
Pcnx2	A	G	8: 125,773,833	L1506P	probably damaging	Het
Prl2c5	T	A	13: 13,189,424	H88Q	probably damaging	Het
Psmb2	A	G	4: 126,687,032	Y73C	probably damaging	Het
Rbl1	A	T	2: 157,169,971	V688D	probably benign	Het
Rhox2g	C	A	X: 37,643,212		probably benign	Het
Rmnd5a	G	A	6: 71,398,455	L80F	probably benign	Het
Ryr2	T	C	13: 11,687,879		probably benign	Het
Sele	T	A	1: 164,050,810	S239R	probably damaging	Het
Siglecf	A	T	7: 43,355,920	R435*	probably null	Het
Slc23a1	C	T	18: 35,622,623	V407M	possibly damaging	Het
Slc6a14	G	A	X: 21,721,568	V122I	probably benign	Het
Spdl1	T	A	11: 34,813,607	E466D	unknown	Het
Stau2	A	G	1: 16,440,372	F121L	probably benign	Het
Stxbp1	A	T	2: 32,794,636	S594T	probably benign	Het
Sufu	G	A	19: 46,454,720		probably benign	Het
Tap2	G	T	17: 34,211,915	V330L	probably benign	Het
Tbc1d1	T	C	5: 64,264,432	L389P	probably damaging	Het
Tbx2	A	G	11: 85,834,766	E181G	probably damaging	Het
Tmem56	A	G	3: 121,207,291	V231A	probably benign	Het
Tmprss9	G	T	10: 80,890,445	A510S	probably benign	Het
Tnxb	G	A	17: 34,671,577	C298Y	probably damaging	Het
Tril	G	T	6: 53,818,027	R737S	probably damaging	Het
Ugt2a3	A	T	5: 87,327,146	L413Q	probably damaging	Het
Vcan	A	G	13: 89,657,556	I2335T	probably damaging	Het
Vmn2r28	T	C	7: 5,481,545	N552S	possibly damaging	Het
Zc3h7a	C	T	16: 11,161,026	R95H	probably damaging	Het
Zfp598	A	G	17: 24,679,649	N474S	probably benign	Het
Zpld1	T	C	16: 55,248,334	D138G	probably damaging	Het

Other mutations in Mslnl

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01621:Mslnl	APN	17	25743667	unclassified	probably benign
IGL01629:Mslnl	APN	17	25744775	missense	possibly damaging	0.95
IGL02084:Mslnl	APN	17	25746151	missense	probably benign	0.07
IGL02408:Mslnl	APN	17	25747998	missense	possibly damaging	0.80
IGL02726:Mslnl	APN	17	25744103	critical splice donor site	probably null
IGL03387:Mslnl	APN	17	25744077	missense	probably benign	0.06
R0561:Mslnl	UTSW	17	25743203	nonsense	probably null
R0881:Mslnl	UTSW	17	25742965	missense	possibly damaging	0.82
R1295:Mslnl	UTSW	17	25743240	missense	probably damaging	1.00
R1582:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1629:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1630:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1631:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1632:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1794:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1850:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1866:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1876:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1914:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2166:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2241:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2243:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2247:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2282:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2284:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2852:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2867:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2867:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2877:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2878:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2919:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2920:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3026:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3405:Mslnl	UTSW	17	25746181	missense	probably damaging	1.00
R3406:Mslnl	UTSW	17	25746181	missense	probably damaging	1.00
R3411:Mslnl	UTSW	17	25744517	missense	probably benign	0.05
R3434:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3546:Mslnl	UTSW	17	25744969	missense	probably damaging	0.98
R3612:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3729:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3730:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3802:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3804:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3894:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3895:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4454:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4455:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4456:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4457:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4561:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4562:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4564:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4600:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4601:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4610:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4704:Mslnl	UTSW	17	25738978	missense	possibly damaging	0.73
R5155:Mslnl	UTSW	17	25738968	nonsense	probably null
R5257:Mslnl	UTSW	17	25746165	missense	probably benign	0.00
R5456:Mslnl	UTSW	17	25743159	missense	probably damaging	0.98
R5645:Mslnl	UTSW	17	25737842	missense	possibly damaging	0.95
R6007:Mslnl	UTSW	17	25746775	missense	probably benign	0.00
R6083:Mslnl	UTSW	17	25737902	missense	possibly damaging	0.83
R6142:Mslnl	UTSW	17	25744557	missense	probably damaging	1.00
R6761:Mslnl	UTSW	17	25746073	missense	probably damaging	1.00
R7058:Mslnl	UTSW	17	25743212	missense	probably benign	0.03
R7156:Mslnl	UTSW	17	25743210	missense	probably benign	0.20
R7467:Mslnl	UTSW	17	25736921	start codon destroyed	probably benign	0.33
R7687:Mslnl	UTSW	17	25743183	missense	probably damaging	0.97
R7807:Mslnl	UTSW	17	25746777	missense	probably benign	0.03
R8682:Mslnl	UTSW	17	25746988	missense	probably benign
R8735:Mslnl	UTSW	17	25745088	missense	probably benign	0.09
R8742:Mslnl	UTSW	17	25745073	missense	probably damaging	1.00
R9208:Mslnl	UTSW	17	25742720	missense	possibly damaging	0.94
R9264:Mslnl	UTSW	17	25742532	intron	probably benign
RF007:Mslnl	UTSW	17	25743228	missense	possibly damaging	0.87

Predicted Primers

PCR Primer
(F):5'- AACTTGCCCAACCAGAGAGTTGCC -3'
(R):5'- CTGTCCTGGGTTCAAACCTCAAGC -3'

Sequencing Primer
(F):5'- ACAGCCTTGGCCTGTCTG -3'
(R):5'- AAGCCCCTTGCATCTTATCTG -3'

Posted On

2014-02-18