Mutagenetix > Incidental Mutation

Incidental Mutation 'R1420:Or8g35'

160054

Institutional Source

Beutler Lab

Gene Symbol

Or8g35

Ensembl Gene

ENSMUSG00000063176

Gene Name

olfactory receptor family 8 subfamily G member 35

Synonyms

Olfr955, MOR171-50, GA_x6K02T2PVTD-33167297-33166353, MOR171-34

MMRRC Submission

039476-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.079) question?

Stock #

R1420 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

39381076-39382020 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 39381289 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Histidine to Glutamine at position 244 (H244Q)

Ref Sequence

ENSEMBL: ENSMUSP00000151246 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000073946] [ENSMUST00000220176]

AlphaFold

Q9EQ97

Predicted Effect

probably damaging
Transcript: ENSMUST00000073946
AA Change: H244Q

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000073602
Gene: ENSMUSG00000063176
AA Change: H244Q

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	308	1.2e-46	PFAM
Pfam:7tm_1	41	290	1.2e-19	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000216492

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000219910

Predicted Effect

probably damaging
Transcript: ENSMUST00000220176
AA Change: H244Q

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

Coding Region Coverage

1x: 98.9%
3x: 98.0%
10x: 95.6%
20x: 90.6%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 52 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aldh1l2	A	G	10: 83,331,799 (GRCm39)	Y669H	probably damaging	Het
Amotl2	A	T	9: 102,601,982 (GRCm39)	M409L	possibly damaging	Het
Ankrd35	A	G	3: 96,592,054 (GRCm39)	E780G	probably benign	Het
Brwd1	T	C	16: 95,837,234 (GRCm39)	Y866C	probably damaging	Het
Cul4b	G	C	X: 37,653,918 (GRCm39)		probably null	Het
Daw1	A	T	1: 83,137,548 (GRCm39)	Y10F	possibly damaging	Het
Dgki	A	T	6: 37,027,204 (GRCm39)		probably null	Het
Dnah3	A	T	7: 119,551,202 (GRCm39)	V3039E	probably damaging	Het
Ercc4	C	T	16: 12,948,073 (GRCm39)	T340I	probably benign	Het
Fam83b	T	C	9: 76,399,894 (GRCm39)	N403S	possibly damaging	Het
Fbh1	A	G	2: 11,772,493 (GRCm39)	F63L	probably benign	Het
Foxm1	G	A	6: 128,349,884 (GRCm39)	R395H	possibly damaging	Het
Gtpbp4	C	T	13: 9,023,298 (GRCm39)	A589T	probably benign	Het
Ifna7	C	A	4: 88,734,906 (GRCm39)	H148N	probably damaging	Het
Il23r	T	C	6: 67,463,181 (GRCm39)	Y104C	probably damaging	Het
Il31ra	A	T	13: 112,668,286 (GRCm39)	W347R	probably damaging	Het
Ints7	T	G	1: 191,345,169 (GRCm39)	F620V	possibly damaging	Het
Iqcd	T	C	5: 120,738,860 (GRCm39)	L226P	probably damaging	Het
Jak3	G	T	8: 72,134,182 (GRCm39)	R428L	possibly damaging	Het
Kif28	C	A	1: 179,529,962 (GRCm39)	C733F	probably damaging	Het
Klkb1	C	A	8: 45,729,183 (GRCm39)	C347F	probably damaging	Het
Ksr2	A	G	5: 117,552,904 (GRCm39)	E4G	probably benign	Het
Lama1	T	C	17: 68,097,942 (GRCm39)	L1774P	probably damaging	Het
Lrriq3	A	G	3: 154,893,349 (GRCm39)	E350G	probably benign	Het
Nav3	G	A	10: 109,659,115 (GRCm39)	A834V	probably benign	Het
Ncoa6	G	A	2: 155,263,073 (GRCm39)	Q454*	probably null	Het
Nfatc2	C	A	2: 168,346,585 (GRCm39)	M836I	probably benign	Het
Nphp3	G	T	9: 103,913,092 (GRCm39)		probably null	Het
Olfml2b	C	T	1: 170,496,596 (GRCm39)	T409M	probably benign	Het
Oprk1	A	G	1: 5,672,544 (GRCm39)	K227R	probably damaging	Het
Pate1	A	T	9: 35,596,505 (GRCm39)	W87R	probably damaging	Het
Pcnx4	T	C	12: 72,602,760 (GRCm39)	Y341H	probably benign	Het
Pde4c	T	A	8: 71,201,066 (GRCm39)	H421Q	probably damaging	Het
Pglyrp4	T	C	3: 90,636,021 (GRCm39)	V82A	probably damaging	Het
Pnmt	G	T	11: 98,278,502 (GRCm39)	R156L	probably benign	Het
Prdm9	C	A	17: 15,764,638 (GRCm39)	C714F	probably damaging	Het
Pwp1	T	C	10: 85,712,402 (GRCm39)	V80A	probably damaging	Het
Pxdn	T	C	12: 30,052,067 (GRCm39)	L568P	probably damaging	Het
Rasgrp4	C	A	7: 28,839,770 (GRCm39)	Q161K	probably damaging	Het
Rnf138	A	G	18: 21,159,159 (GRCm39)	E193G	probably damaging	Het
Slc16a6	A	G	11: 109,345,772 (GRCm39)	V413A	probably damaging	Het
Slc7a15	T	C	12: 8,584,442 (GRCm39)	T363A	probably benign	Het
Sufu	T	C	19: 46,385,623 (GRCm39)	S28P	probably benign	Het
Tex19.1	T	C	11: 121,037,872 (GRCm39)	S77P	probably damaging	Het
Tmc7	A	T	7: 118,165,440 (GRCm39)	Y91*	probably null	Het
Ttbk2	C	G	2: 120,576,393 (GRCm39)	R792S	probably benign	Het
Tyw1	T	C	5: 130,303,586 (GRCm39)		probably null	Het
U2surp	A	G	9: 95,344,856 (GRCm39)	S907P	probably benign	Het
Vmn2r115	ATCTTCT	ATCT	17: 23,578,962 (GRCm39)		probably benign	Het
Vps50	T	A	6: 3,588,007 (GRCm39)	L660*	probably null	Het
Wdr55	A	G	18: 36,893,392 (GRCm39)	E18G	probably benign	Het
Wipi1	A	G	11: 109,469,198 (GRCm39)	V331A	probably benign	Het

Other mutations in Or8g35

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00157:Or8g35	APN	9	39,381,539 (GRCm39)	missense	probably benign	0.00
IGL02550:Or8g35	APN	9	39,381,842 (GRCm39)	missense	probably benign	0.42
IGL02743:Or8g35	APN	9	39,381,542 (GRCm39)	missense	probably benign	0.10
R0029:Or8g35	UTSW	9	39,381,956 (GRCm39)	missense	probably benign	0.06
R0329:Or8g35	UTSW	9	39,381,852 (GRCm39)	missense	possibly damaging	0.52
R0610:Or8g35	UTSW	9	39,381,119 (GRCm39)	missense	probably damaging	0.98
R1636:Or8g35	UTSW	9	39,381,215 (GRCm39)	missense	probably benign	0.03
R1937:Or8g35	UTSW	9	39,381,333 (GRCm39)	missense	possibly damaging	0.63
R2655:Or8g35	UTSW	9	39,381,924 (GRCm39)	missense	probably benign
R2944:Or8g35	UTSW	9	39,381,234 (GRCm39)	missense	possibly damaging	0.52
R3788:Or8g35	UTSW	9	39,381,365 (GRCm39)	missense	probably benign	0.03
R4829:Or8g35	UTSW	9	39,381,663 (GRCm39)	missense	probably damaging	0.99
R5625:Or8g35	UTSW	9	39,381,099 (GRCm39)	missense	probably benign
R6168:Or8g35	UTSW	9	39,381,953 (GRCm39)	missense	probably damaging	1.00
R6383:Or8g35	UTSW	9	39,381,926 (GRCm39)	missense	probably damaging	1.00
R6418:Or8g35	UTSW	9	39,381,112 (GRCm39)	missense	probably benign	0.07
R6645:Or8g35	UTSW	9	39,381,562 (GRCm39)	missense	probably benign	0.03
R7062:Or8g35	UTSW	9	39,381,353 (GRCm39)	missense	probably benign
R7765:Or8g35	UTSW	9	39,381,612 (GRCm39)	missense	probably benign	0.28
R7847:Or8g35	UTSW	9	39,381,801 (GRCm39)	missense	probably benign	0.42
R8122:Or8g35	UTSW	9	39,381,822 (GRCm39)	missense	probably damaging	1.00
R9578:Or8g35	UTSW	9	39,381,201 (GRCm39)	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- TTCTCAGGCTGTAGATCATGGGATTCA -3'
(R):5'- GCTTAATCAGTGCCACAGCTCACA -3'

Sequencing Primer
(F):5'- TCATGGGATTCAACATAGGCAC -3'
(R):5'- TCACACAGTCTTCCTGCTAAG -3'

Posted On

2014-03-14