Mutagenetix > Incidental Mutation

Incidental Mutation 'R1398:Vps13d'

160223

Institutional Source

Beutler Lab

Gene Symbol

Vps13d

Ensembl Gene

ENSMUSG00000020220

Gene Name

vacuolar protein sorting 13D

Synonyms

MMRRC Submission

039460-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R1398 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

144972622-145195005 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 145099983 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Leucine to Glutamine at position 1726 (L1726Q)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000020441] [ENSMUST00000036579]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000020441
AA Change: L2875Q

PolyPhen 2 Score 0.961 (Sensitivity: 0.78; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000020441
Gene: ENSMUSG00000020220
AA Change: L2875Q

Domain	Start	End	E-Value	Type
Pfam:Chorein_N	2	118	1.8e-37	PFAM
low complexity region	407	423	N/A	INTRINSIC
low complexity region	534	555	N/A	INTRINSIC
coiled coil region	665	685	N/A	INTRINSIC
low complexity region	765	781	N/A	INTRINSIC
low complexity region	1316	1329	N/A	INTRINSIC
low complexity region	1590	1603	N/A	INTRINSIC
Blast:IL1	1605	1726	2e-6	BLAST
low complexity region	1868	1883	N/A	INTRINSIC
low complexity region	2128	2141	N/A	INTRINSIC
UBA	2632	2669	3.73e-5	SMART
low complexity region	2674	2684	N/A	INTRINSIC
low complexity region	2707	2718	N/A	INTRINSIC
low complexity region	2866	2884	N/A	INTRINSIC
low complexity region	2973	2983	N/A	INTRINSIC
Pfam:DUF1162	3246	3530	1.1e-110	PFAM
low complexity region	3797	3810	N/A	INTRINSIC
low complexity region	3913	3921	N/A	INTRINSIC
low complexity region	4119	4132	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000036579
AA Change: L2906Q

PolyPhen 2 Score 0.295 (Sensitivity: 0.91; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000043240
Gene: ENSMUSG00000020220
AA Change: L2906Q

Domain	Start	End	E-Value	Type
Pfam:Chorein_N	2	116	3.5e-35	PFAM
Pfam:VPS13	131	353	9.6e-57	PFAM
low complexity region	407	423	N/A	INTRINSIC
low complexity region	534	555	N/A	INTRINSIC
Pfam:VPS13_mid_rpt	608	896	4.3e-35	PFAM
low complexity region	1316	1329	N/A	INTRINSIC
low complexity region	1590	1603	N/A	INTRINSIC
Blast:IL1	1605	1726	2e-6	BLAST
low complexity region	1868	1883	N/A	INTRINSIC
low complexity region	2128	2141	N/A	INTRINSIC
UBA	2632	2669	3.73e-5	SMART
low complexity region	2674	2684	N/A	INTRINSIC
low complexity region	2707	2718	N/A	INTRINSIC
low complexity region	2891	2909	N/A	INTRINSIC
low complexity region	2998	3008	N/A	INTRINSIC
Pfam:SHR-BD	3271	3555	4.2e-86	PFAM
low complexity region	3822	3835	N/A	INTRINSIC
low complexity region	3938	3946	N/A	INTRINSIC
Pfam:VPS13_C	3978	4126	4.8e-24	PFAM
low complexity region	4144	4157	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000185113
AA Change: L1726Q

Meta Mutation Damage Score

0.6676

Coding Region Coverage

1x: 99.0%
3x: 98.1%
10x: 95.6%
20x: 90.7%

Validation Efficiency

96% (75/78)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a protein belonging to the vacuolar-protein-sorting-13 gene family. In yeast, vacuolar-protein-sorting-13 proteins are involved in trafficking of membrane proteins between the trans-Golgi network and the prevacuolar compartment. While several transcript variants may exist for this gene, the full-length natures of only two have been described to date. These two represent the major variants of this gene and encode distinct isoforms. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a transgenic gene disruption exhibit embryonic lethality at E7. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 74 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4931408C20Rik	G	T	1: 26,685,341	Q253K	possibly damaging	Het
9330182L06Rik	A	G	5: 9,380,297	Y69C	probably damaging	Het
Abca17	T	C	17: 24,328,537	K288E	probably damaging	Het
Aldh3a2	T	C	11: 61,256,736		probably null	Het
Anks1b	A	G	10: 90,050,029	T196A	probably damaging	Het
Anks6	T	A	4: 47,044,926	T327S	possibly damaging	Het
Bdh2	T	C	3: 135,295,296		probably benign	Het
C4b	T	A	17: 34,730,719		probably benign	Het
Cacna2d1	G	A	5: 16,357,766	V847I	possibly damaging	Het
Cadps	G	T	14: 12,449,822	T1129K	probably damaging	Het
Cdc45	A	G	16: 18,781,971		probably benign	Het
Cep63	A	T	9: 102,603,086		probably benign	Het
Chil4	T	A	3: 106,219,509		probably null	Het
Cnot11	A	G	1: 39,545,180	R478G	probably damaging	Het
Cyp2c67	A	G	19: 39,638,625	S254P	probably damaging	Het
Dnah11	T	A	12: 118,057,106	K87*	probably null	Het
Dpy19l2	T	A	9: 24,581,263		probably benign	Het
Dsc1	A	T	18: 20,088,336	I694N	probably damaging	Het
Ehd4	A	T	2: 120,127,600	I168K	probably benign	Het
Eif4e	A	T	3: 138,546,375	N25Y	probably damaging	Het
Eme2	G	A	17: 24,892,918	S263F	probably damaging	Het
Fam160b1	T	A	19: 57,372,926		probably benign	Het
Fgfrl1	T	A	5: 108,706,281		probably benign	Het
Gm3159	T	A	14: 4,398,586	Y92*	probably null	Het
Gm4922	T	A	10: 18,783,748	S409C	possibly damaging	Het
Gmcl1	G	A	6: 86,714,262		probably benign	Het
Grsf1	A	G	5: 88,665,847	Y231H	probably benign	Het
Heatr4	T	A	12: 83,967,621	H614L	possibly damaging	Het
Hoxa13	C	G	6: 52,260,647		probably benign	Het
Hoxa13	G	C	6: 52,260,648		probably benign	Het
Isg20l2	C	A	3: 87,938,754	L325I	probably benign	Het
Kalrn	A	G	16: 34,212,820	Y879H	probably damaging	Het
Kcnk10	C	A	12: 98,436,226	W318L	probably damaging	Het
Kctd1	T	C	18: 15,062,597	E323G	possibly damaging	Het
Kif4	G	T	X: 100,689,097	A492S	probably benign	Het
Krtap4-1	T	C	11: 99,627,732	T151A	unknown	Het
Ldlr	A	T	9: 21,739,542	Q449L	probably benign	Het
Lepr	T	A	4: 101,792,019	D872E	probably damaging	Het
Lgals12	C	T	19: 7,603,957		probably benign	Het
Lrig3	C	T	10: 126,003,088	P488L	probably benign	Het
Lrrc4b	A	C	7: 44,462,452	I583L	probably benign	Het
Lyst	T	C	13: 13,740,536	S3272P	possibly damaging	Het
March2	T	C	17: 33,696,122	H166R	probably damaging	Het
Mtbp	C	A	15: 55,577,537	Y373*	probably null	Het
Myh2	C	T	11: 67,185,287	H767Y	probably benign	Het
Ncam1	G	A	9: 49,517,589		probably benign	Het
Neb	T	A	2: 52,289,646	N1282Y	probably damaging	Het
Nectin3	A	G	16: 46,448,756	Y428H	possibly damaging	Het
Nrros	A	T	16: 32,143,144	I649N	probably damaging	Het
Nvl	A	T	1: 181,097,126		probably benign	Het
Olfr495	T	A	7: 108,395,501	V127E	probably damaging	Het
Pms1	A	T	1: 53,207,276	V368E	possibly damaging	Het
Polq	T	A	16: 37,062,495	S1674T	possibly damaging	Het
Ppp1r21	T	C	17: 88,542,879	V31A	probably damaging	Het
Rev3l	T	A	10: 39,821,583	V692E	probably benign	Het
Robo4	T	C	9: 37,408,076		probably null	Het
Rps6kc1	T	C	1: 190,800,015	I597V	probably damaging	Het
Rtel1	T	A	2: 181,335,865		probably null	Het
Scn9a	A	G	2: 66,484,586	M1587T	probably benign	Het
Sec31b	T	A	19: 44,523,665	I597F	probably benign	Het
Skint5	T	C	4: 113,779,071	N650S	unknown	Het
Slc22a28	G	T	19: 8,130,202	S167*	probably null	Het
Slfn1	T	A	11: 83,121,142	M28K	probably damaging	Het
Smc6	T	C	12: 11,271,879		probably benign	Het
Sox8	T	C	17: 25,567,883	H282R	probably benign	Het
Syngr3	C	A	17: 24,686,440	V161L	probably benign	Het
Trak1	C	T	9: 121,454,359	S397F	probably damaging	Het
Uso1	C	T	5: 92,181,468	A405V	probably benign	Het
Uvrag	G	T	7: 99,065,820	Y190*	probably null	Het
Vwf	A	T	6: 125,603,457	Q556L	probably benign	Het
Wdr70	T	A	15: 8,035,844	M246L	probably benign	Het
Yipf3	T	C	17: 46,251,446	F285S	probably damaging	Het
Zdhhc13	G	A	7: 48,826,873	G579R	probably damaging	Het
Zdhhc18	G	C	4: 133,627,297	F125L	probably benign	Het

Other mutations in Vps13d

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00163:Vps13d	APN	4	145,168,540 (GRCm38)	missense	probably damaging	0.98
IGL00484:Vps13d	APN	4	145,126,575 (GRCm38)	missense	probably benign	0.04
IGL00591:Vps13d	APN	4	145,190,559 (GRCm38)	missense	possibly damaging	0.95
IGL00816:Vps13d	APN	4	145,155,994 (GRCm38)	missense	probably benign	0.00
IGL00835:Vps13d	APN	4	145,160,652 (GRCm38)	missense	probably damaging	0.97
IGL00847:Vps13d	APN	4	145,085,408 (GRCm38)	missense	probably benign	0.26
IGL01084:Vps13d	APN	4	145,154,955 (GRCm38)	missense	probably benign	0.00
IGL01116:Vps13d	APN	4	144,972,750 (GRCm38)	unclassified	probably benign
IGL01150:Vps13d	APN	4	145,149,275 (GRCm38)	missense	probably benign
IGL01329:Vps13d	APN	4	145,156,206 (GRCm38)	missense	possibly damaging	0.69
IGL01338:Vps13d	APN	4	145,088,322 (GRCm38)	missense	probably damaging	1.00
IGL01583:Vps13d	APN	4	145,045,088 (GRCm38)	missense	probably damaging	1.00
IGL01598:Vps13d	APN	4	145,016,901 (GRCm38)	missense	probably benign	0.21
IGL01620:Vps13d	APN	4	145,094,867 (GRCm38)	missense	possibly damaging	0.70
IGL01636:Vps13d	APN	4	145,075,048 (GRCm38)	missense	probably damaging	1.00
IGL01723:Vps13d	APN	4	145,173,145 (GRCm38)	missense	possibly damaging	0.84
IGL01895:Vps13d	APN	4	145,156,266 (GRCm38)	missense	possibly damaging	0.57
IGL01981:Vps13d	APN	4	145,086,747 (GRCm38)	missense	probably damaging	0.99
IGL02192:Vps13d	APN	4	145,148,858 (GRCm38)	missense	probably benign	0.02
IGL02197:Vps13d	APN	4	145,128,309 (GRCm38)	missense	probably benign	0.01
IGL02209:Vps13d	APN	4	145,156,101 (GRCm38)	missense	probably damaging	0.97
IGL02219:Vps13d	APN	4	145,168,146 (GRCm38)	missense	probably benign	0.00
IGL02377:Vps13d	APN	4	145,156,364 (GRCm38)	missense	probably damaging	1.00
IGL02404:Vps13d	APN	4	145,148,735 (GRCm38)	missense	probably damaging	1.00
IGL02552:Vps13d	APN	4	145,173,137 (GRCm38)	missense	possibly damaging	0.46
IGL02651:Vps13d	APN	4	145,164,559 (GRCm38)	missense	probably benign	0.02
IGL02708:Vps13d	APN	4	145,128,280 (GRCm38)	missense	probably benign	0.12
IGL02811:Vps13d	APN	4	145,131,765 (GRCm38)	missense	possibly damaging	0.55
IGL02821:Vps13d	APN	4	145,148,762 (GRCm38)	missense	probably damaging	0.98
IGL02838:Vps13d	APN	4	145,075,025 (GRCm38)	missense	probably benign	0.31
IGL02968:Vps13d	APN	4	145,122,498 (GRCm38)	missense	probably benign	0.32
IGL03176:Vps13d	APN	4	145,074,963 (GRCm38)	missense	probably benign	0.16
IGL03352:Vps13d	APN	4	145,167,502 (GRCm38)	missense	possibly damaging	0.49
IGL03374:Vps13d	APN	4	145,108,575 (GRCm38)	missense	possibly damaging	0.70
IGL03375:Vps13d	APN	4	145,091,947 (GRCm38)	missense	probably damaging	1.00
IGL03383:Vps13d	APN	4	145,168,319 (GRCm38)	critical splice acceptor site	probably null
IGL03411:Vps13d	APN	4	145,149,324 (GRCm38)	missense	probably damaging	1.00
broken	UTSW	4	145,086,735 (GRCm38)	missense
demotion	UTSW	4	145,138,613 (GRCm38)	missense
BB008:Vps13d	UTSW	4	145,096,284 (GRCm38)	nonsense	probably null
BB018:Vps13d	UTSW	4	145,096,284 (GRCm38)	nonsense	probably null
PIT4283001:Vps13d	UTSW	4	145,108,588 (GRCm38)	missense
PIT4434001:Vps13d	UTSW	4	145,155,247 (GRCm38)	missense
R0069:Vps13d	UTSW	4	145,062,563 (GRCm38)	missense	probably benign	0.09
R0069:Vps13d	UTSW	4	145,062,563 (GRCm38)	missense	probably benign	0.09
R0076:Vps13d	UTSW	4	145,164,694 (GRCm38)	splice site	probably benign
R0211:Vps13d	UTSW	4	145,114,778 (GRCm38)	missense	probably benign	0.08
R0219:Vps13d	UTSW	4	145,105,909 (GRCm38)	missense	probably benign	0.01
R0284:Vps13d	UTSW	4	145,144,802 (GRCm38)	missense	probably benign	0.01
R0345:Vps13d	UTSW	4	145,117,625 (GRCm38)	missense	possibly damaging	0.81
R0400:Vps13d	UTSW	4	145,065,827 (GRCm38)	missense	probably benign	0.00
R0417:Vps13d	UTSW	4	144,976,560 (GRCm38)	missense	probably benign	0.19
R0538:Vps13d	UTSW	4	145,045,095 (GRCm38)	missense	probably damaging	1.00
R0560:Vps13d	UTSW	4	145,054,190 (GRCm38)	missense	probably damaging	1.00
R0627:Vps13d	UTSW	4	145,087,184 (GRCm38)	missense	probably damaging	1.00
R0707:Vps13d	UTSW	4	145,155,932 (GRCm38)	missense	probably damaging	1.00
R0782:Vps13d	UTSW	4	145,126,625 (GRCm38)	splice site	probably benign
R0925:Vps13d	UTSW	4	145,156,551 (GRCm38)	missense	probably damaging	1.00
R0993:Vps13d	UTSW	4	145,117,692 (GRCm38)	nonsense	probably null
R1135:Vps13d	UTSW	4	145,155,589 (GRCm38)	missense	probably benign	0.01
R1165:Vps13d	UTSW	4	145,126,471 (GRCm38)	missense	probably benign
R1263:Vps13d	UTSW	4	145,170,348 (GRCm38)	missense	probably benign	0.01
R1397:Vps13d	UTSW	4	145,141,334 (GRCm38)	missense	probably damaging	1.00
R1521:Vps13d	UTSW	4	145,105,861 (GRCm38)	missense	probably benign	0.00
R1522:Vps13d	UTSW	4	145,098,172 (GRCm38)	splice site	probably null
R1725:Vps13d	UTSW	4	145,143,260 (GRCm38)	missense	possibly damaging	0.90
R1759:Vps13d	UTSW	4	145,155,857 (GRCm38)	missense	probably benign
R1826:Vps13d	UTSW	4	145,155,003 (GRCm38)	missense	probably damaging	0.96
R1900:Vps13d	UTSW	4	145,126,606 (GRCm38)	missense	probably benign	0.23
R1943:Vps13d	UTSW	4	145,155,857 (GRCm38)	missense	probably benign
R1955:Vps13d	UTSW	4	145,156,143 (GRCm38)	missense	probably damaging	1.00
R2008:Vps13d	UTSW	4	145,155,243 (GRCm38)	missense	probably benign	0.00
R2013:Vps13d	UTSW	4	145,108,508 (GRCm38)	missense	probably damaging	0.99
R2014:Vps13d	UTSW	4	145,108,508 (GRCm38)	missense	probably damaging	0.99
R2038:Vps13d	UTSW	4	145,181,115 (GRCm38)	critical splice donor site	probably null
R2108:Vps13d	UTSW	4	145,075,047 (GRCm38)	missense	probably damaging	0.99
R2130:Vps13d	UTSW	4	145,156,101 (GRCm38)	missense	probably benign	0.17
R2134:Vps13d	UTSW	4	145,148,339 (GRCm38)	missense	probably benign	0.00
R2168:Vps13d	UTSW	4	145,087,323 (GRCm38)	splice site	probably benign
R2220:Vps13d	UTSW	4	145,178,320 (GRCm38)	missense	probably damaging	1.00
R2240:Vps13d	UTSW	4	145,110,895 (GRCm38)	missense	possibly damaging	0.70
R2332:Vps13d	UTSW	4	145,148,686 (GRCm38)	missense	probably benign
R2357:Vps13d	UTSW	4	145,074,977 (GRCm38)	frame shift	probably null
R2365:Vps13d	UTSW	4	145,087,324 (GRCm38)	splice site	probably benign
R2571:Vps13d	UTSW	4	145,149,136 (GRCm38)	missense	probably benign	0.20
R3149:Vps13d	UTSW	4	145,126,577 (GRCm38)	missense	possibly damaging	0.70
R3150:Vps13d	UTSW	4	145,086,790 (GRCm38)	missense	probably damaging	0.98
R3547:Vps13d	UTSW	4	145,074,975 (GRCm38)	missense	probably damaging	0.99
R3716:Vps13d	UTSW	4	145,075,726 (GRCm38)	missense	probably damaging	1.00
R3718:Vps13d	UTSW	4	145,075,726 (GRCm38)	missense	probably damaging	1.00
R3725:Vps13d	UTSW	4	145,115,648 (GRCm38)	splice site	probably benign
R3794:Vps13d	UTSW	4	145,085,437 (GRCm38)	splice site	probably benign
R3875:Vps13d	UTSW	4	145,190,544 (GRCm38)	missense	probably damaging	1.00
R3948:Vps13d	UTSW	4	145,141,340 (GRCm38)	missense	probably damaging	1.00
R3953:Vps13d	UTSW	4	145,148,880 (GRCm38)	missense	probably damaging	1.00
R4021:Vps13d	UTSW	4	145,075,061 (GRCm38)	missense	possibly damaging	0.90
R4323:Vps13d	UTSW	4	145,152,778 (GRCm38)	missense	probably benign	0.28
R4346:Vps13d	UTSW	4	145,072,529 (GRCm38)	intron	probably benign
R4509:Vps13d	UTSW	4	145,062,602 (GRCm38)	missense	probably damaging	1.00
R4613:Vps13d	UTSW	4	145,131,655 (GRCm38)	missense	possibly damaging	0.95
R4657:Vps13d	UTSW	4	145,074,842 (GRCm38)	missense	probably damaging	1.00
R4680:Vps13d	UTSW	4	145,108,510 (GRCm38)	missense	possibly damaging	0.94
R4688:Vps13d	UTSW	4	145,178,212 (GRCm38)	missense	probably benign
R4797:Vps13d	UTSW	4	145,054,155 (GRCm38)	missense	probably damaging	1.00
R4798:Vps13d	UTSW	4	145,178,056 (GRCm38)	missense	probably damaging	0.98
R4817:Vps13d	UTSW	4	145,069,165 (GRCm38)	missense	probably damaging	1.00
R4839:Vps13d	UTSW	4	145,085,430 (GRCm38)	missense	possibly damaging	0.95
R4860:Vps13d	UTSW	4	145,087,161 (GRCm38)	missense	probably benign
R4860:Vps13d	UTSW	4	145,087,161 (GRCm38)	missense	probably benign
R4869:Vps13d	UTSW	4	145,128,042 (GRCm38)	missense	probably damaging	1.00
R4904:Vps13d	UTSW	4	145,155,445 (GRCm38)	missense	probably damaging	1.00
R4912:Vps13d	UTSW	4	145,155,857 (GRCm38)	missense	probably benign
R4916:Vps13d	UTSW	4	144,983,393 (GRCm38)	missense	probably damaging	1.00
R4976:Vps13d	UTSW	4	145,105,898 (GRCm38)	missense	possibly damaging	0.82
R5029:Vps13d	UTSW	4	145,156,282 (GRCm38)	missense	probably benign	0.02
R5049:Vps13d	UTSW	4	145,086,766 (GRCm38)	missense	probably damaging	1.00
R5077:Vps13d	UTSW	4	145,088,241 (GRCm38)	missense	probably damaging	0.98
R5119:Vps13d	UTSW	4	145,105,898 (GRCm38)	missense	possibly damaging	0.82
R5227:Vps13d	UTSW	4	145,181,207 (GRCm38)	splice site	probably null
R5291:Vps13d	UTSW	4	145,062,569 (GRCm38)	missense	probably damaging	0.99
R5344:Vps13d	UTSW	4	145,178,334 (GRCm38)	missense	probably damaging	0.98
R5348:Vps13d	UTSW	4	145,065,889 (GRCm38)	missense	probably damaging	0.99
R5478:Vps13d	UTSW	4	145,167,550 (GRCm38)	missense	probably damaging	0.99
R5632:Vps13d	UTSW	4	145,074,882 (GRCm38)	missense	probably damaging	0.99
R5642:Vps13d	UTSW	4	145,170,302 (GRCm38)	missense	possibly damaging	0.66
R5712:Vps13d	UTSW	4	145,087,173 (GRCm38)	missense	probably benign	0.07
R5747:Vps13d	UTSW	4	145,168,283 (GRCm38)	missense	probably benign	0.00
R5752:Vps13d	UTSW	4	145,148,970 (GRCm38)	missense	probably benign	0.06
R5804:Vps13d	UTSW	4	145,100,070 (GRCm38)	missense	probably benign	0.03
R5917:Vps13d	UTSW	4	145,100,010 (GRCm38)	missense	probably damaging	0.96
R5932:Vps13d	UTSW	4	145,045,041 (GRCm38)	missense	possibly damaging	0.71
R5940:Vps13d	UTSW	4	145,074,975 (GRCm38)	missense	probably benign	0.09
R5978:Vps13d	UTSW	4	145,122,611 (GRCm38)	missense	probably benign
R6031:Vps13d	UTSW	4	145,168,509 (GRCm38)	missense	probably benign	0.01
R6031:Vps13d	UTSW	4	145,168,509 (GRCm38)	missense	probably benign	0.01
R6143:Vps13d	UTSW	4	145,148,565 (GRCm38)	missense	possibly damaging	0.95
R6174:Vps13d	UTSW	4	144,975,193 (GRCm38)	nonsense	probably null
R6191:Vps13d	UTSW	4	145,149,348 (GRCm38)	missense	probably damaging	1.00
R6198:Vps13d	UTSW	4	145,148,990 (GRCm38)	missense	probably benign	0.28
R6374:Vps13d	UTSW	4	145,122,681 (GRCm38)	missense	probably damaging	1.00
R6379:Vps13d	UTSW	4	145,088,258 (GRCm38)	missense	probably benign
R6388:Vps13d	UTSW	4	145,155,574 (GRCm38)	missense	probably benign	0.06
R6418:Vps13d	UTSW	4	145,092,280 (GRCm38)	missense	probably damaging	0.98
R6466:Vps13d	UTSW	4	145,057,495 (GRCm38)	missense	possibly damaging	0.47
R6602:Vps13d	UTSW	4	145,103,664 (GRCm38)	intron	probably benign
R6604:Vps13d	UTSW	4	145,181,124 (GRCm38)	missense	probably damaging	1.00
R7051:Vps13d	UTSW	4	145,163,344 (GRCm38)	missense	probably benign	0.00
R7052:Vps13d	UTSW	4	145,163,344 (GRCm38)	missense	probably benign	0.00
R7103:Vps13d	UTSW	4	145,115,492 (GRCm38)	missense
R7231:Vps13d	UTSW	4	145,057,462 (GRCm38)	missense
R7246:Vps13d	UTSW	4	145,156,050 (GRCm38)	missense
R7339:Vps13d	UTSW	4	145,121,368 (GRCm38)	missense
R7409:Vps13d	UTSW	4	145,141,254 (GRCm38)	missense
R7419:Vps13d	UTSW	4	145,115,503 (GRCm38)	missense
R7424:Vps13d	UTSW	4	145,148,747 (GRCm38)	missense
R7439:Vps13d	UTSW	4	145,105,856 (GRCm38)	missense
R7440:Vps13d	UTSW	4	145,128,411 (GRCm38)	missense
R7528:Vps13d	UTSW	4	145,091,922 (GRCm38)	missense
R7547:Vps13d	UTSW	4	145,057,538 (GRCm38)	missense
R7558:Vps13d	UTSW	4	145,154,580 (GRCm38)	missense
R7699:Vps13d	UTSW	4	145,085,405 (GRCm38)	missense
R7729:Vps13d	UTSW	4	145,075,052 (GRCm38)	missense
R7789:Vps13d	UTSW	4	145,100,065 (GRCm38)	missense
R7813:Vps13d	UTSW	4	145,178,063 (GRCm38)	nonsense	probably null
R7834:Vps13d	UTSW	4	145,108,573 (GRCm38)	missense
R7840:Vps13d	UTSW	4	145,103,676 (GRCm38)	missense
R7880:Vps13d	UTSW	4	145,181,114 (GRCm38)	critical splice donor site	probably null
R7912:Vps13d	UTSW	4	145,173,127 (GRCm38)	missense
R7915:Vps13d	UTSW	4	145,086,819 (GRCm38)	missense
R7931:Vps13d	UTSW	4	145,096,284 (GRCm38)	nonsense	probably null
R8021:Vps13d	UTSW	4	145,148,675 (GRCm38)	missense
R8048:Vps13d	UTSW	4	145,155,567 (GRCm38)	missense
R8057:Vps13d	UTSW	4	144,975,183 (GRCm38)	missense
R8063:Vps13d	UTSW	4	145,114,757 (GRCm38)	missense
R8131:Vps13d	UTSW	4	145,156,137 (GRCm38)	missense
R8190:Vps13d	UTSW	4	145,152,751 (GRCm38)	missense
R8226:Vps13d	UTSW	4	145,149,290 (GRCm38)	missense
R8241:Vps13d	UTSW	4	145,148,477 (GRCm38)	missense
R8254:Vps13d	UTSW	4	144,983,312 (GRCm38)	splice site	probably benign
R8305:Vps13d	UTSW	4	145,092,288 (GRCm38)	missense
R8415:Vps13d	UTSW	4	145,091,979 (GRCm38)	missense
R8460:Vps13d	UTSW	4	145,170,439 (GRCm38)	intron	probably benign
R8487:Vps13d	UTSW	4	145,155,247 (GRCm38)	missense	probably benign	0.11
R8543:Vps13d	UTSW	4	145,016,783 (GRCm38)	nonsense	probably null
R8679:Vps13d	UTSW	4	145,085,407 (GRCm38)	missense
R8716:Vps13d	UTSW	4	145,075,778 (GRCm38)	missense
R8749:Vps13d	UTSW	4	145,138,613 (GRCm38)	missense
R8772:Vps13d	UTSW	4	145,075,032 (GRCm38)	missense
R8788:Vps13d	UTSW	4	145,086,735 (GRCm38)	missense
R8789:Vps13d	UTSW	4	145,069,173 (GRCm38)	missense
R8836:Vps13d	UTSW	4	145,156,078 (GRCm38)	missense
R8874:Vps13d	UTSW	4	145,155,202 (GRCm38)	missense
R8918:Vps13d	UTSW	4	145,046,303 (GRCm38)	missense
R9129:Vps13d	UTSW	4	145,171,679 (GRCm38)	missense
R9220:Vps13d	UTSW	4	145,056,488 (GRCm38)	missense
R9233:Vps13d	UTSW	4	145,152,774 (GRCm38)	missense
R9234:Vps13d	UTSW	4	145,149,222 (GRCm38)	missense
R9256:Vps13d	UTSW	4	145,155,804 (GRCm38)	missense
R9350:Vps13d	UTSW	4	145,155,763 (GRCm38)	missense
R9398:Vps13d	UTSW	4	145,170,386 (GRCm38)	nonsense	probably null
R9415:Vps13d	UTSW	4	145,069,957 (GRCm38)	missense
R9438:Vps13d	UTSW	4	145,131,744 (GRCm38)	missense
R9469:Vps13d	UTSW	4	145,054,121 (GRCm38)	missense
R9487:Vps13d	UTSW	4	145,081,299 (GRCm38)	critical splice donor site	probably null
R9524:Vps13d	UTSW	4	145,096,244 (GRCm38)	missense
R9616:Vps13d	UTSW	4	145,098,131 (GRCm38)	missense
R9655:Vps13d	UTSW	4	145,086,735 (GRCm38)	missense
R9709:Vps13d	UTSW	4	145,149,345 (GRCm38)	missense
R9767:Vps13d	UTSW	4	145,152,736 (GRCm38)	missense
R9773:Vps13d	UTSW	4	145,092,049 (GRCm38)	missense
R9779:Vps13d	UTSW	4	145,072,402 (GRCm38)	missense
R9796:Vps13d	UTSW	4	145,127,935 (GRCm38)	critical splice donor site	probably null
X0021:Vps13d	UTSW	4	145,155,025 (GRCm38)	missense	probably damaging	0.99
Z1176:Vps13d	UTSW	4	145,107,067 (GRCm38)	missense
Z1177:Vps13d	UTSW	4	145,178,296 (GRCm38)	missense
Z1177:Vps13d	UTSW	4	145,154,908 (GRCm38)	missense

Predicted Primers

PCR Primer
(F):5'- AGTATGGAGCCTACAGAAGCCTGG -3'
(R):5'- CAGCAAACGTGCAGTTCCTTTTCTC -3'

Sequencing Primer
(F):5'- ACAGAAGCCTGGGATGACTTTTC -3'
(R):5'- CGTCACACAGGAGACAGC -3'

Posted On

2014-03-14