|Institutional Source||Beutler Lab|
|Gene Name||kinesin family member 4|
|Synonyms||N-5 kinesin, Kns4, D330050K22Rik|
|Is this an essential gene?||Possibly essential (E-score: 0.556)|
|Stock #||R1398 (G1)|
|Chromosomal Location||100625737-100727214 bp(+) (GRCm38)|
|Type of Mutation||missense|
|DNA Base Change (assembly)||G to T at 100689097 bp|
|Amino Acid Change||Alanine to Serine at position 492 (A492S)|
|Ref Sequence||ENSEMBL: ENSMUSP00000048383 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000048962]|
|Predicted Effect||probably benign
AA Change: A492S
PolyPhen 2 Score 0.003 (Sensitivity: 0.98; Specificity: 0.44)
AA Change: A492S
|Meta Mutation Damage Score||0.0336|
|Coding Region Coverage||
|Validation Efficiency||96% (75/78)|
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the kinesin 4 subfamily of kinesin related proteins. The encoded protein is an ATP dependent microtubule-based motor protein that is involved in the intracellular transport of membranous organelles. This protein also associates with condensed chromosome arms and may be involved in maintaining chromosome integrity during mitosis. This protein may also be involved in the organization of the central spindle prior to cytokinesis. A pseudogene of this gene is found on chromosome X.[provided by RefSeq, Mar 2010]
PHENOTYPE: Male chimeras hemizygous for a gene trapped allele appear normal at E9.5. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Kif4||
(F):5'- TCAATACCAGCGATTGATTCCACCC -3'
(R):5'- ATGAAACGCGATGCTGCTGAGG -3'
(F):5'- GCTAAAGTGCAGCCATCTATG -3'
(R):5'- GAGGAGTCTCAGCGTTCATTACC -3'