Mutagenetix > Incidental Mutation

Incidental Mutation 'R1405:Grip2'

160519

Institutional Source

Beutler Lab

Gene Symbol

Grip2

Ensembl Gene

ENSMUSG00000030098

Gene Name

glutamate receptor interacting protein 2

Synonyms

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R1405 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

91738490-91804231 bp(-) (GRCm39)

Type of Mutation

splice site

DNA Base Change (assembly)

A to T at 91765133 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000124709 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000159684] [ENSMUST00000159684] [ENSMUST00000159684] [ENSMUST00000159684] [ENSMUST00000161566] [ENSMUST00000161566] [ENSMUST00000162293] [ENSMUST00000162293] [ENSMUST00000162300] [ENSMUST00000162300] [ENSMUST00000162300] [ENSMUST00000162300]

AlphaFold

G3XA20

Predicted Effect

probably null
Transcript: ENSMUST00000159684

SMART Domains

Protein: ENSMUSP00000125047
Gene: ENSMUSG00000030098

Domain	Start	End	E-Value	Type
low complexity region	25	36	N/A	INTRINSIC
PDZ	62	136	1.12e-12	SMART
PDZ	161	239	3.8e-15	SMART
PDZ	262	337	7.9e-13	SMART
low complexity region	385	390	N/A	INTRINSIC
PDZ	426	506	2.18e-15	SMART
PDZ	527	602	3.86e-16	SMART
PDZ	625	699	1.38e-17	SMART
low complexity region	778	793	N/A	INTRINSIC
low complexity region	867	878	N/A	INTRINSIC
PDZ	910	982	2.95e-12	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000159684

SMART Domains

Protein: ENSMUSP00000125047
Gene: ENSMUSG00000030098

Domain	Start	End	E-Value	Type
low complexity region	25	36	N/A	INTRINSIC
PDZ	62	136	1.12e-12	SMART
PDZ	161	239	3.8e-15	SMART
PDZ	262	337	7.9e-13	SMART
low complexity region	385	390	N/A	INTRINSIC
PDZ	426	506	2.18e-15	SMART
PDZ	527	602	3.86e-16	SMART
PDZ	625	699	1.38e-17	SMART
low complexity region	778	793	N/A	INTRINSIC
low complexity region	867	878	N/A	INTRINSIC
PDZ	910	982	2.95e-12	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000159684

SMART Domains

Protein: ENSMUSP00000125047
Gene: ENSMUSG00000030098

Domain	Start	End	E-Value	Type
low complexity region	25	36	N/A	INTRINSIC
PDZ	62	136	1.12e-12	SMART
PDZ	161	239	3.8e-15	SMART
PDZ	262	337	7.9e-13	SMART
low complexity region	385	390	N/A	INTRINSIC
PDZ	426	506	2.18e-15	SMART
PDZ	527	602	3.86e-16	SMART
PDZ	625	699	1.38e-17	SMART
low complexity region	778	793	N/A	INTRINSIC
low complexity region	867	878	N/A	INTRINSIC
PDZ	910	982	2.95e-12	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000159684

SMART Domains

Protein: ENSMUSP00000125047
Gene: ENSMUSG00000030098

Domain	Start	End	E-Value	Type
low complexity region	25	36	N/A	INTRINSIC
PDZ	62	136	1.12e-12	SMART
PDZ	161	239	3.8e-15	SMART
PDZ	262	337	7.9e-13	SMART
low complexity region	385	390	N/A	INTRINSIC
PDZ	426	506	2.18e-15	SMART
PDZ	527	602	3.86e-16	SMART
PDZ	625	699	1.38e-17	SMART
low complexity region	778	793	N/A	INTRINSIC
low complexity region	867	878	N/A	INTRINSIC
PDZ	910	982	2.95e-12	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000161545

SMART Domains

Protein: ENSMUSP00000125538
Gene: ENSMUSG00000030098

Domain	Start	End	E-Value	Type
low complexity region	22	33	N/A	INTRINSIC
PDB:2QT5\|B	42	89	3e-9	PDB
SCOP:d1lcya1	47	89	2e-7	SMART
low complexity region	103	119	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000161545

SMART Domains

Protein: ENSMUSP00000125538
Gene: ENSMUSG00000030098

Domain	Start	End	E-Value	Type
low complexity region	22	33	N/A	INTRINSIC
PDB:2QT5\|B	42	89	3e-9	PDB
SCOP:d1lcya1	47	89	2e-7	SMART
low complexity region	103	119	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000161566

SMART Domains

Protein: ENSMUSP00000123941
Gene: ENSMUSG00000030098

Domain	Start	End	E-Value	Type
low complexity region	25	36	N/A	INTRINSIC
PDZ	62	136	9.96e-10	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000161566

SMART Domains

Protein: ENSMUSP00000123941
Gene: ENSMUSG00000030098

Domain	Start	End	E-Value	Type
low complexity region	25	36	N/A	INTRINSIC
PDZ	62	136	9.96e-10	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000162293

SMART Domains

Protein: ENSMUSP00000124717
Gene: ENSMUSG00000030098

Domain	Start	End	E-Value	Type
PDZ	10	84	1.12e-12	SMART
PDZ	109	187	3.8e-15	SMART
PDZ	210	285	7.9e-13	SMART
low complexity region	336	348	N/A	INTRINSIC
low complexity region	374	379	N/A	INTRINSIC
PDZ	415	495	2.18e-15	SMART
PDZ	516	591	3.86e-16	SMART
PDZ	614	688	1.38e-17	SMART
low complexity region	767	782	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000162293

SMART Domains

Protein: ENSMUSP00000124717
Gene: ENSMUSG00000030098

Domain	Start	End	E-Value	Type
PDZ	10	84	1.12e-12	SMART
PDZ	109	187	3.8e-15	SMART
PDZ	210	285	7.9e-13	SMART
low complexity region	336	348	N/A	INTRINSIC
low complexity region	374	379	N/A	INTRINSIC
PDZ	415	495	2.18e-15	SMART
PDZ	516	591	3.86e-16	SMART
PDZ	614	688	1.38e-17	SMART
low complexity region	767	782	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000162300

SMART Domains

Protein: ENSMUSP00000124709
Gene: ENSMUSG00000030098

Domain	Start	End	E-Value	Type
low complexity region	25	36	N/A	INTRINSIC
PDZ	62	136	1.12e-12	SMART
PDZ	161	239	3.8e-15	SMART
PDZ	262	337	7.9e-13	SMART
low complexity region	388	400	N/A	INTRINSIC
low complexity region	426	431	N/A	INTRINSIC
PDZ	467	547	2.18e-15	SMART
PDZ	568	643	3.86e-16	SMART
PDZ	666	740	1.38e-17	SMART
low complexity region	819	834	N/A	INTRINSIC
low complexity region	908	919	N/A	INTRINSIC
PDZ	951	1023	2.95e-12	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000162300

SMART Domains

Protein: ENSMUSP00000124709
Gene: ENSMUSG00000030098

Domain	Start	End	E-Value	Type
low complexity region	25	36	N/A	INTRINSIC
PDZ	62	136	1.12e-12	SMART
PDZ	161	239	3.8e-15	SMART
PDZ	262	337	7.9e-13	SMART
low complexity region	388	400	N/A	INTRINSIC
low complexity region	426	431	N/A	INTRINSIC
PDZ	467	547	2.18e-15	SMART
PDZ	568	643	3.86e-16	SMART
PDZ	666	740	1.38e-17	SMART
low complexity region	819	834	N/A	INTRINSIC
low complexity region	908	919	N/A	INTRINSIC
PDZ	951	1023	2.95e-12	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000162300

SMART Domains

Protein: ENSMUSP00000124709
Gene: ENSMUSG00000030098

Domain	Start	End	E-Value	Type
low complexity region	25	36	N/A	INTRINSIC
PDZ	62	136	1.12e-12	SMART
PDZ	161	239	3.8e-15	SMART
PDZ	262	337	7.9e-13	SMART
low complexity region	388	400	N/A	INTRINSIC
low complexity region	426	431	N/A	INTRINSIC
PDZ	467	547	2.18e-15	SMART
PDZ	568	643	3.86e-16	SMART
PDZ	666	740	1.38e-17	SMART
low complexity region	819	834	N/A	INTRINSIC
low complexity region	908	919	N/A	INTRINSIC
PDZ	951	1023	2.95e-12	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000162300

SMART Domains

Protein: ENSMUSP00000124709
Gene: ENSMUSG00000030098

Domain	Start	End	E-Value	Type
low complexity region	25	36	N/A	INTRINSIC
PDZ	62	136	1.12e-12	SMART
PDZ	161	239	3.8e-15	SMART
PDZ	262	337	7.9e-13	SMART
low complexity region	388	400	N/A	INTRINSIC
low complexity region	426	431	N/A	INTRINSIC
PDZ	467	547	2.18e-15	SMART
PDZ	568	643	3.86e-16	SMART
PDZ	666	740	1.38e-17	SMART
low complexity region	819	834	N/A	INTRINSIC
low complexity region	908	919	N/A	INTRINSIC
PDZ	951	1023	2.95e-12	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000205964

Coding Region Coverage

1x: 99.0%
3x: 96.7%
10x: 83.5%
20x: 53.1%

Validation Efficiency

MGI Phenotype

PHENOTYPE: Homozygous null mice are born in numbers expected by the Mendelian ratio and show no overt phenotype. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 38 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Arap1	G	A	7: 101,047,643 (GRCm39)		probably null	Het
Asb8	G	A	15: 98,039,248 (GRCm39)	H51Y	possibly damaging	Het
Capn10	T	G	1: 92,872,744 (GRCm39)	V490G	probably benign	Het
Ccdc146	G	A	5: 21,604,730 (GRCm39)	S36L	probably benign	Het
Celsr1	C	T	15: 85,789,635 (GRCm39)		probably null	Het
Clvs2	C	A	10: 33,389,256 (GRCm39)	*328L	probably null	Het
Cul9	C	G	17: 46,833,101 (GRCm39)	A1326P	probably damaging	Het
Dstn	A	G	2: 143,780,356 (GRCm39)	K19E	probably damaging	Het
Ehmt2	T	A	17: 35,125,553 (GRCm39)	H134Q	probably benign	Het
Faah	G	A	4: 115,858,345 (GRCm39)	P411S	probably damaging	Het
Fn1	A	G	1: 71,681,237 (GRCm39)	F364L	probably damaging	Het
Gmnc	A	T	16: 26,779,196 (GRCm39)	N270K	possibly damaging	Het
Hmg20a	A	T	9: 56,384,587 (GRCm39)	Q119L	possibly damaging	Het
Ipo7	T	C	7: 109,629,048 (GRCm39)	I106T	probably benign	Het
Ipo7	C	T	7: 109,638,456 (GRCm39)	P241L	probably damaging	Het
Katnb1	T	C	8: 95,824,801 (GRCm39)	Y574H	probably damaging	Het
Larp6	A	C	9: 60,644,849 (GRCm39)	M330L	probably benign	Het
Lrrc8e	T	C	8: 4,281,754 (GRCm39)	Y30H	probably damaging	Het
Nop56	T	C	2: 130,119,868 (GRCm39)	V420A	probably benign	Het
Nrg1	T	C	8: 32,407,855 (GRCm39)	D126G	probably benign	Het
Prdm1	T	A	10: 44,315,961 (GRCm39)	N725I	probably damaging	Het
Prl3a1	A	G	13: 27,459,051 (GRCm39)		probably null	Het
Psmd2	T	C	16: 20,471,034 (GRCm39)	L59P	possibly damaging	Het
Ptgdr2	T	C	19: 10,918,395 (GRCm39)	V304A	probably benign	Het
Rasa3	A	G	8: 13,638,027 (GRCm39)	V339A	possibly damaging	Het
Sec24c	G	A	14: 20,742,593 (GRCm39)		probably null	Het
Serpinb9e	A	G	13: 33,444,009 (GRCm39)	D343G	probably benign	Het
Sfi1	TCGC	TC	11: 3,096,254 (GRCm39)		probably null	Het
Sfi1	CCTCTC	CCTCTCTC	11: 3,127,419 (GRCm39)		probably benign	Het
Stab1	A	C	14: 30,870,958 (GRCm39)	V1297G	probably benign	Het
Stk4	C	T	2: 163,942,448 (GRCm39)	T360M	probably benign	Het
Tmprss2	G	A	16: 97,398,005 (GRCm39)	T57I	probably benign	Het
Tnrc6a	A	G	7: 122,770,301 (GRCm39)	D697G	probably damaging	Het
Vwa5b2	T	A	16: 20,423,066 (GRCm39)	D1021E	probably benign	Het
Wdr46	C	A	17: 34,168,057 (GRCm39)	P543Q	probably damaging	Het
Zfp1005	T	A	2: 150,109,620 (GRCm39)	Y103*	probably null	Het
Zfp287	T	A	11: 62,619,137 (GRCm39)	D119V	probably damaging	Het
Zxdc	A	G	6: 90,361,225 (GRCm39)	S737G	possibly damaging	Het

Other mutations in Grip2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01141:Grip2	APN	6	91,759,878 (GRCm39)	missense	probably benign	0.00
IGL01748:Grip2	APN	6	91,741,724 (GRCm39)	missense	probably damaging	1.00
IGL01838:Grip2	APN	6	91,741,744 (GRCm39)	missense	possibly damaging	0.92
IGL02392:Grip2	APN	6	91,764,276 (GRCm39)	missense	probably damaging	1.00
IGL02620:Grip2	APN	6	91,755,587 (GRCm39)	missense	possibly damaging	0.93
IGL02862:Grip2	APN	6	91,765,085 (GRCm39)	missense	probably damaging	0.98
IGL03027:Grip2	APN	6	91,755,852 (GRCm39)	missense	probably benign	0.02
IGL03180:Grip2	APN	6	91,762,742 (GRCm39)	splice site	probably benign
R0265:Grip2	UTSW	6	91,750,773 (GRCm39)	critical splice donor site	probably null
R0448:Grip2	UTSW	6	91,756,194 (GRCm39)	missense	probably damaging	1.00
R0597:Grip2	UTSW	6	91,773,178 (GRCm39)	intron	probably benign
R1405:Grip2	UTSW	6	91,765,133 (GRCm39)	splice site	probably null
R1466:Grip2	UTSW	6	91,765,424 (GRCm39)	missense	probably damaging	0.98
R1466:Grip2	UTSW	6	91,765,424 (GRCm39)	missense	probably damaging	0.98
R1664:Grip2	UTSW	6	91,742,233 (GRCm39)	missense	probably damaging	1.00
R1703:Grip2	UTSW	6	91,754,379 (GRCm39)	missense	probably damaging	1.00
R1793:Grip2	UTSW	6	91,760,623 (GRCm39)	missense	probably benign	0.03
R1951:Grip2	UTSW	6	91,760,829 (GRCm39)	missense	probably damaging	1.00
R2001:Grip2	UTSW	6	91,756,831 (GRCm39)	missense	probably benign	0.00
R4730:Grip2	UTSW	6	91,762,693 (GRCm39)	makesense	probably null
R4754:Grip2	UTSW	6	91,756,173 (GRCm39)	missense	probably damaging	0.97
R4754:Grip2	UTSW	6	91,756,163 (GRCm39)	missense	probably damaging	1.00
R4773:Grip2	UTSW	6	91,759,413 (GRCm39)	missense	possibly damaging	0.80
R5135:Grip2	UTSW	6	91,750,897 (GRCm39)	missense	possibly damaging	0.89
R5213:Grip2	UTSW	6	91,756,812 (GRCm39)	missense	probably benign	0.04
R5972:Grip2	UTSW	6	91,784,262 (GRCm39)	missense	probably benign	0.01
R6176:Grip2	UTSW	6	91,756,832 (GRCm39)	missense	probably benign	0.00
R6188:Grip2	UTSW	6	91,740,514 (GRCm39)	missense	probably damaging	1.00
R6289:Grip2	UTSW	6	91,755,852 (GRCm39)	missense	probably benign	0.02
R6345:Grip2	UTSW	6	91,742,369 (GRCm39)	missense	possibly damaging	0.91
R6348:Grip2	UTSW	6	91,757,419 (GRCm39)	missense	probably damaging	0.99
R6394:Grip2	UTSW	6	91,764,182 (GRCm39)	missense	probably damaging	1.00
R6658:Grip2	UTSW	6	91,763,472 (GRCm39)	missense	probably damaging	1.00
R7065:Grip2	UTSW	6	91,760,550 (GRCm39)	critical splice donor site	probably null
R7074:Grip2	UTSW	6	91,761,689 (GRCm39)	missense	probably benign	0.24
R7308:Grip2	UTSW	6	91,755,669 (GRCm39)	missense	possibly damaging	0.74
R7607:Grip2	UTSW	6	91,765,393 (GRCm39)	missense	probably benign
R7617:Grip2	UTSW	6	91,742,031 (GRCm39)	splice site	probably null
R7970:Grip2	UTSW	6	91,763,513 (GRCm39)	missense	probably benign	0.07
R8221:Grip2	UTSW	6	91,762,665 (GRCm39)	missense	possibly damaging	0.90
R8549:Grip2	UTSW	6	91,750,769 (GRCm39)	splice site	probably null
R8838:Grip2	UTSW	6	91,762,721 (GRCm39)	utr 3 prime	probably benign
R8962:Grip2	UTSW	6	91,754,391 (GRCm39)	missense	probably damaging	1.00
R9430:Grip2	UTSW	6	91,784,265 (GRCm39)	missense	probably benign	0.05
R9699:Grip2	UTSW	6	91,742,318 (GRCm39)	missense	probably benign
R9732:Grip2	UTSW	6	91,761,686 (GRCm39)	missense	probably damaging	0.99
RF003:Grip2	UTSW	6	91,760,574 (GRCm39)	missense	probably benign	0.02
Z1176:Grip2	UTSW	6	91,740,491 (GRCm39)	missense	possibly damaging	0.55

Predicted Primers

PCR Primer
(F):5'- GACATGGAAGGGTACATGGCCTTG -3'
(R):5'- GTTCTAGTGACTCGTGACGTAGCG -3'

Sequencing Primer
(F):5'- TGTGGCAGACATCAAGCTC -3'
(R):5'- GTGACGTAGCGGCACTG -3'

Posted On

2014-03-14