Mutagenetix > Incidental Mutation

Incidental Mutation 'R1447:Zfp976'

160701

Institutional Source

Beutler Lab

Gene Symbol

Zfp976

Ensembl Gene

ENSMUSG00000074158

Gene Name

zinc finger protein 976

Synonyms

9830147E19Rik

MMRRC Submission

039502-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.092) question?

Stock #

R1447 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

42258950-42292012 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to T at 42262023 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Proline to Threonine at position 605 (P605T)

Ref Sequence

ENSEMBL: ENSMUSP00000141023 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000098503] [ENSMUST00000187616]

AlphaFold

E9Q981

Predicted Effect

unknown
Transcript: ENSMUST00000098503
AA Change: P606T

SMART Domains

Protein: ENSMUSP00000096105
Gene: ENSMUSG00000074158
AA Change: P606T

Domain	Start	End	E-Value	Type
KRAB	4	66	1.73e-18	SMART
ZnF_C2H2	131	153	7.78e-3	SMART
ZnF_C2H2	159	181	1.67e-2	SMART
ZnF_C2H2	187	209	5.9e-3	SMART
ZnF_C2H2	215	237	1.38e-3	SMART
ZnF_C2H2	243	265	8.94e-3	SMART
ZnF_C2H2	271	293	2.24e-3	SMART
ZnF_C2H2	299	321	1.03e-2	SMART
ZnF_C2H2	327	349	1.58e-3	SMART
ZnF_C2H2	355	377	7.9e-4	SMART
ZnF_C2H2	383	405	5.9e-3	SMART
ZnF_C2H2	411	433	2.57e-3	SMART
ZnF_C2H2	439	461	3.16e-3	SMART
ZnF_C2H2	467	489	1.26e-2	SMART
ZnF_C2H2	495	517	8.34e-3	SMART
ZnF_C2H2	523	545	3.63e-3	SMART
ZnF_C2H2	551	573	4.79e-3	SMART
ZnF_C2H2	579	601	9.73e-4	SMART
ZnF_C2H2	607	629	3.63e-3	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000107994

Predicted Effect

possibly damaging
Transcript: ENSMUST00000187616
AA Change: P605T

PolyPhen 2 Score 0.785 (Sensitivity: 0.85; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000141023
Gene: ENSMUSG00000074158
AA Change: P605T

Domain	Start	End	E-Value	Type
KRAB	3	65	7.4e-21	SMART
ZnF_C2H2	130	152	3.2e-5	SMART
ZnF_C2H2	158	180	7.1e-5	SMART
ZnF_C2H2	186	208	2.4e-5	SMART
ZnF_C2H2	214	236	5.7e-6	SMART
ZnF_C2H2	242	264	3.8e-5	SMART
ZnF_C2H2	270	292	9.7e-6	SMART
ZnF_C2H2	298	320	4.2e-5	SMART
ZnF_C2H2	326	348	6.6e-6	SMART
ZnF_C2H2	354	376	3.3e-6	SMART
ZnF_C2H2	382	404	2.5e-5	SMART
ZnF_C2H2	410	432	1e-5	SMART
ZnF_C2H2	438	460	1.3e-5	SMART
ZnF_C2H2	466	488	5.2e-5	SMART
ZnF_C2H2	494	516	3.6e-5	SMART
ZnF_C2H2	522	544	1.6e-5	SMART
ZnF_C2H2	550	572	2e-5	SMART
ZnF_C2H2	578	600	4e-6	SMART
ZnF_C2H2	606	628	1.5e-5	SMART

Coding Region Coverage

1x: 98.8%
3x: 97.6%
10x: 93.8%
20x: 84.2%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 58 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adamts12	A	T	15: 11,263,447 (GRCm39)	D603V	probably benign	Het
Ahnak	A	T	19: 8,984,446 (GRCm39)	E1910V	probably damaging	Het
Aif1	G	A	17: 35,391,127 (GRCm39)	P44L	probably benign	Het
Amotl1	A	G	9: 14,467,038 (GRCm39)	V704A	probably benign	Het
Ankrd50	A	T	3: 38,509,691 (GRCm39)	V892E	probably damaging	Het
Aoc1	G	A	6: 48,883,176 (GRCm39)	V351I	probably benign	Het
Atp6v1b1	T	C	6: 83,734,924 (GRCm39)	V412A	possibly damaging	Het
Atp8b2	A	T	3: 89,851,477 (GRCm39)	I906N	probably damaging	Het
Bltp1	T	C	3: 37,019,735 (GRCm39)	L2048P	probably damaging	Het
Bnc2	A	G	4: 84,211,457 (GRCm39)	V304A	probably benign	Het
Btaf1	A	G	19: 36,969,854 (GRCm39)	D1176G	probably benign	Het
C1qtnf3	G	A	15: 10,952,735 (GRCm39)	G66R	probably damaging	Het
Ccdc162	T	C	10: 41,456,243 (GRCm39)	E360G	probably damaging	Het
Csmd1	C	A	8: 15,975,306 (GRCm39)	G2968*	probably null	Het
Cyp2j7	A	T	4: 96,083,530 (GRCm39)	F473L	possibly damaging	Het
Ddx24	T	C	12: 103,390,566 (GRCm39)	K142E	possibly damaging	Het
Dnah1	A	T	14: 31,028,855 (GRCm39)	M625K	probably benign	Het
Dnah9	A	T	11: 65,999,308 (GRCm39)	Y944N	possibly damaging	Het
Eef1akmt1	T	A	14: 57,803,441 (GRCm39)	K38*	probably null	Het
Enpp2	C	T	15: 54,782,994 (GRCm39)		probably null	Het
Eps8l1	A	G	7: 4,477,055 (GRCm39)	E508G	probably damaging	Het
Etaa1	A	T	11: 17,896,625 (GRCm39)	D497E	possibly damaging	Het
Fam181b	C	T	7: 92,729,368 (GRCm39)	A47V	probably damaging	Het
Fubp3	T	C	2: 31,490,559 (GRCm39)	V221A	probably damaging	Het
Golga2	T	C	2: 32,187,788 (GRCm39)	V191A	possibly damaging	Het
Haus5	G	T	7: 30,361,216 (GRCm39)		probably null	Het
Hydin	T	C	8: 111,249,798 (GRCm39)	L2247P	probably damaging	Het
Lama5	A	C	2: 179,827,671 (GRCm39)	I2197S	probably damaging	Het
Mapre3	C	T	5: 31,019,151 (GRCm39)		probably benign	Het
Mast2	C	A	4: 116,169,210 (GRCm39)	M733I	probably benign	Het
Mphosph10	A	T	7: 64,030,698 (GRCm39)	F514I	probably damaging	Het
Mterf3	A	G	13: 67,065,103 (GRCm39)	L266P	probably damaging	Het
Nup205	A	G	6: 35,192,120 (GRCm39)	D1114G	probably benign	Het
Or1ak2	T	G	2: 36,827,788 (GRCm39)	V219G	possibly damaging	Het
Or1e21	A	G	11: 73,344,700 (GRCm39)	F113L	probably benign	Het
Phf24	G	T	4: 42,938,232 (GRCm39)	E119*	probably null	Het
Pik3r5	G	A	11: 68,385,003 (GRCm39)	R636Q	probably benign	Het
Plaat3	G	A	19: 7,556,598 (GRCm39)	R133H	probably benign	Het
Rida	T	A	15: 34,488,757 (GRCm39)	Q45L	possibly damaging	Het
Ros1	T	C	10: 51,974,954 (GRCm39)	T1544A	possibly damaging	Het
Rpl7	T	A	1: 16,172,821 (GRCm39)	Y166F	probably benign	Het
Rps6ka5	A	G	12: 100,544,084 (GRCm39)	I338T	probably benign	Het
Scn3a	A	T	2: 65,300,324 (GRCm39)	N1347K	probably damaging	Het
Serpinb6d	A	G	13: 33,854,739 (GRCm39)	D238G	probably benign	Het
Sh3rf2	T	A	18: 42,234,736 (GRCm39)	I173N	probably benign	Het
Smarcc2	T	C	10: 128,305,660 (GRCm39)		probably null	Het
Thoc2l	T	C	5: 104,670,070 (GRCm39)	S1531P	possibly damaging	Het
Thsd4	T	C	9: 59,904,496 (GRCm39)	N567D	probably benign	Het
Tmprss13	C	A	9: 45,239,878 (GRCm39)	P62Q	unknown	Het
Tmprss7	T	C	16: 45,501,033 (GRCm39)	Q256R	probably benign	Het
Tssc4	A	G	7: 142,623,892 (GRCm39)	T67A	probably benign	Het
Tssk5	G	A	15: 76,256,304 (GRCm39)	Q372*	probably null	Het
Usp47	G	T	7: 111,673,775 (GRCm39)		probably null	Het
Utp15	A	G	13: 98,389,386 (GRCm39)	I304T	possibly damaging	Het
Vmn1r35	A	C	6: 66,655,890 (GRCm39)	V93G	probably benign	Het
Zfhx3	A	C	8: 109,675,076 (GRCm39)	Q2042P	probably benign	Het
Zfp532	C	A	18: 65,758,061 (GRCm39)	R665S	probably damaging	Het
Zfpl1	A	G	19: 6,132,649 (GRCm39)	V125A	possibly damaging	Het

Other mutations in Zfp976

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00465:Zfp976	APN	7	42,263,109 (GRCm39)	missense	unknown
IGL01102:Zfp976	APN	7	42,263,333 (GRCm39)	nonsense	probably null
IGL01111:Zfp976	APN	7	42,265,711 (GRCm39)	missense	probably damaging	0.99
IGL01628:Zfp976	APN	7	42,261,935 (GRCm39)	missense	unknown
IGL02008:Zfp976	APN	7	42,263,656 (GRCm39)	splice site	probably benign
IGL02548:Zfp976	APN	7	42,261,953 (GRCm39)	missense	unknown
R0190:Zfp976	UTSW	7	42,291,948 (GRCm39)	start gained	probably benign
R0685:Zfp976	UTSW	7	42,263,141 (GRCm39)	missense	probably damaging	0.98
R1310:Zfp976	UTSW	7	42,262,610 (GRCm39)	missense	probably damaging	1.00
R1353:Zfp976	UTSW	7	42,265,442 (GRCm39)	missense	probably damaging	0.99
R1569:Zfp976	UTSW	7	42,262,806 (GRCm39)	missense	probably damaging	1.00
R1702:Zfp976	UTSW	7	42,265,424 (GRCm39)	missense	possibly damaging	0.86
R1829:Zfp976	UTSW	7	42,265,735 (GRCm39)	missense	probably damaging	1.00
R1939:Zfp976	UTSW	7	42,263,105 (GRCm39)	missense	unknown
R1978:Zfp976	UTSW	7	42,263,265 (GRCm39)	missense	probably damaging	1.00
R1981:Zfp976	UTSW	7	42,263,046 (GRCm39)	missense	probably damaging	0.99
R2160:Zfp976	UTSW	7	42,263,354 (GRCm39)	missense	probably benign
R2192:Zfp976	UTSW	7	42,262,695 (GRCm39)	missense	probably damaging	1.00
R3121:Zfp976	UTSW	7	42,262,938 (GRCm39)	missense	probably damaging	1.00
R4210:Zfp976	UTSW	7	42,265,749 (GRCm39)	missense	probably damaging	0.99
R4724:Zfp976	UTSW	7	42,262,457 (GRCm39)	missense	possibly damaging	0.91
R4943:Zfp976	UTSW	7	42,261,846 (GRCm39)	unclassified	probably benign
R5047:Zfp976	UTSW	7	42,262,843 (GRCm39)	nonsense	probably null
R5071:Zfp976	UTSW	7	42,262,354 (GRCm39)	nonsense	probably null
R5125:Zfp976	UTSW	7	42,261,925 (GRCm39)	splice site	probably null
R5178:Zfp976	UTSW	7	42,261,925 (GRCm39)	splice site	probably null
R5305:Zfp976	UTSW	7	42,262,902 (GRCm39)	missense	probably benign	0.00
R5777:Zfp976	UTSW	7	42,263,504 (GRCm39)	missense	probably benign	0.00
R6153:Zfp976	UTSW	7	42,263,610 (GRCm39)	missense	probably damaging	0.99
R6694:Zfp976	UTSW	7	42,263,610 (GRCm39)	missense	probably damaging	0.99
R7226:Zfp976	UTSW	7	42,262,684 (GRCm39)	nonsense	probably null
R7479:Zfp976	UTSW	7	42,262,603 (GRCm39)	missense	probably benign	0.01
R7561:Zfp976	UTSW	7	42,265,701 (GRCm39)	missense	probably damaging	1.00
R8178:Zfp976	UTSW	7	42,262,959 (GRCm39)	missense	probably benign	0.03
R8261:Zfp976	UTSW	7	42,262,125 (GRCm39)	missense	unknown
R8715:Zfp976	UTSW	7	42,262,869 (GRCm39)	missense	possibly damaging	0.89
R8921:Zfp976	UTSW	7	42,262,575 (GRCm39)	missense	possibly damaging	0.57
R9168:Zfp976	UTSW	7	42,263,011 (GRCm39)	nonsense	probably null
R9575:Zfp976	UTSW	7	42,262,041 (GRCm39)	missense	unknown
Z1088:Zfp976	UTSW	7	42,262,184 (GRCm39)	missense	possibly damaging	0.71

Predicted Primers

Posted On

2014-03-14