Mutagenetix > Incidental Mutation

Incidental Mutation 'R1441:Vmn2r58'

161008

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r58

Ensembl Gene

ENSMUSG00000090383

Gene Name

vomeronasal 2, receptor 58

Synonyms

EG628422

MMRRC Submission

039496-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.280) question?

Stock #

R1441 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

41486305-41522094 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 41486864 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Isoleucine at position 677 (T677I)

Ref Sequence

ENSEMBL: ENSMUSP00000126966 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000171671]

AlphaFold

K7N6V2

Predicted Effect

probably damaging
Transcript: ENSMUST00000171671
AA Change: T677I

PolyPhen 2 Score 0.997 (Sensitivity: 0.41; Specificity: 0.98)

SMART Domains

Protein: ENSMUSP00000126966
Gene: ENSMUSG00000090383
AA Change: T677I

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	77	471	8e-43	PFAM
Pfam:NCD3G	514	567	1.8e-23	PFAM
Pfam:7tm_3	597	835	2.9e-52	PFAM

Coding Region Coverage

1x: 99.0%
3x: 98.1%
10x: 95.6%
20x: 90.4%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 47 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adamts18	A	T	8: 114,481,194 (GRCm39)		probably null	Het
Ankrd16	T	C	2: 11,783,557 (GRCm39)	L53P	probably damaging	Het
Arsk	A	T	13: 76,223,083 (GRCm39)	N171K	probably benign	Het
Brwd1	A	C	16: 95,867,351 (GRCm39)	C161W	probably damaging	Het
Card9	T	C	2: 26,249,402 (GRCm39)	N53S	probably benign	Het
Ccdc13	A	T	9: 121,642,515 (GRCm39)	V403E	probably benign	Het
Ccdc83	T	A	7: 89,893,351 (GRCm39)	E135D	probably damaging	Het
Ccser1	C	T	6: 62,357,016 (GRCm39)	T818I	probably benign	Het
Cd44	T	A	2: 102,676,763 (GRCm39)	T301S	probably damaging	Het
Eepd1	G	A	9: 25,394,499 (GRCm39)	M254I	probably benign	Het
Ephb4	C	T	5: 137,359,509 (GRCm39)	R360C	probably damaging	Het
Fam149a	G	T	8: 45,808,684 (GRCm39)	Q150K	probably damaging	Het
G6pc2	G	A	2: 69,051,198 (GRCm39)	C97Y	probably damaging	Het
Gcsam	T	A	16: 45,433,401 (GRCm39)	M15K	probably benign	Het
Impdh2	C	A	9: 108,441,975 (GRCm39)	T201K	probably benign	Het
Kdm2b	C	T	5: 123,070,943 (GRCm39)	E379K	probably benign	Het
Mcm3ap	T	C	10: 76,307,000 (GRCm39)	V371A	probably benign	Het
Mink1	T	A	11: 70,497,940 (GRCm39)	N514K	possibly damaging	Het
Mmp12	C	T	9: 7,354,787 (GRCm39)	P330L	probably damaging	Het
Mroh2a	A	G	1: 88,169,353 (GRCm39)	D676G	possibly damaging	Het
Myo1a	C	T	10: 127,555,148 (GRCm39)	P838L	probably benign	Het
Naip5	T	C	13: 100,356,225 (GRCm39)	H1130R	possibly damaging	Het
Ninl	C	A	2: 150,813,044 (GRCm39)	G204V	probably benign	Het
Or12k5	C	A	2: 36,895,131 (GRCm39)	R165L	possibly damaging	Het
Or2a54	T	C	6: 43,092,880 (GRCm39)	V68A	probably benign	Het
Or4k51	T	C	2: 111,585,347 (GRCm39)	F251S	probably damaging	Het
Or5ac20	G	A	16: 59,104,228 (GRCm39)	L211F	probably benign	Het
Or5an11	T	A	19: 12,245,750 (GRCm39)	L52*	probably null	Het
Or8c15	T	C	9: 38,120,777 (GRCm39)	C141R	probably damaging	Het
Phactr4	G	A	4: 132,104,559 (GRCm39)	T256I	probably benign	Het
Pip4p2	A	T	4: 14,892,477 (GRCm39)	I114L	possibly damaging	Het
Ptpn22	G	T	3: 103,781,563 (GRCm39)	W114L	probably damaging	Het
Rasa1	C	T	13: 85,400,540 (GRCm39)		probably null	Het
Rbks	C	T	5: 31,817,341 (GRCm39)	V143I	probably benign	Het
Rbm19	T	C	5: 120,269,241 (GRCm39)	F515L	probably damaging	Het
Ror1	A	G	4: 100,298,180 (GRCm39)	T518A	probably benign	Het
Rpusd4	C	A	9: 35,184,065 (GRCm39)	A240E	probably damaging	Het
Rufy3	T	C	5: 88,780,374 (GRCm39)	L374P	probably damaging	Het
Sf3a3	T	C	4: 124,618,935 (GRCm39)	S299P	probably damaging	Het
Slc7a12	T	G	3: 14,562,414 (GRCm39)	S264A	possibly damaging	Het
Tasor	T	A	14: 27,186,217 (GRCm39)	C805*	probably null	Het
Tm9sf1	T	C	14: 55,873,782 (GRCm39)	Y572C	probably damaging	Het
Tpcn2	G	A	7: 144,813,871 (GRCm39)	S475L	probably benign	Het
Trim17	A	G	11: 58,856,018 (GRCm39)	D25G	probably damaging	Het
Ttn	T	A	2: 76,572,121 (GRCm39)	K26257N	probably damaging	Het
Txndc11	C	A	16: 10,952,414 (GRCm39)		probably benign	Het
Utrn	A	G	10: 12,559,039 (GRCm39)	S1405P	probably damaging	Het

Other mutations in Vmn2r58

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00089:Vmn2r58	APN	7	41,513,854 (GRCm39)	missense	possibly damaging	0.53
IGL00924:Vmn2r58	APN	7	41,486,891 (GRCm39)	missense	probably damaging	1.00
IGL01291:Vmn2r58	APN	7	41,513,935 (GRCm39)	missense	probably benign	0.02
IGL01480:Vmn2r58	APN	7	41,514,116 (GRCm39)	missense	probably benign	0.01
IGL01551:Vmn2r58	APN	7	41,514,703 (GRCm39)	missense	probably damaging	0.97
IGL01591:Vmn2r58	APN	7	41,514,753 (GRCm39)	missense	probably benign	0.03
IGL01940:Vmn2r58	APN	7	41,487,071 (GRCm39)	missense	probably benign	0.00
IGL01994:Vmn2r58	APN	7	41,486,394 (GRCm39)	missense	probably damaging	0.98
IGL02041:Vmn2r58	APN	7	41,514,703 (GRCm39)	missense	probably damaging	0.97
IGL02222:Vmn2r58	APN	7	41,513,449 (GRCm39)	missense	possibly damaging	0.81
IGL02317:Vmn2r58	APN	7	41,486,765 (GRCm39)	missense	possibly damaging	0.89
IGL02614:Vmn2r58	APN	7	41,486,553 (GRCm39)	missense	probably damaging	1.00
IGL02673:Vmn2r58	APN	7	41,514,082 (GRCm39)	missense	possibly damaging	0.90
IGL03323:Vmn2r58	APN	7	41,511,295 (GRCm39)	missense	probably benign	0.06
IGL03337:Vmn2r58	APN	7	41,513,810 (GRCm39)	missense	possibly damaging	0.93
IGL03380:Vmn2r58	APN	7	41,513,874 (GRCm39)	missense	probably benign	0.00
ANU05:Vmn2r58	UTSW	7	41,513,935 (GRCm39)	missense	probably benign	0.02
R0138:Vmn2r58	UTSW	7	41,487,048 (GRCm39)	missense	probably damaging	1.00
R0141:Vmn2r58	UTSW	7	41,511,309 (GRCm39)	missense	probably benign	0.11
R0421:Vmn2r58	UTSW	7	41,514,628 (GRCm39)	missense	probably benign	0.02
R0604:Vmn2r58	UTSW	7	41,510,000 (GRCm39)	missense	possibly damaging	0.78
R0854:Vmn2r58	UTSW	7	41,486,562 (GRCm39)	missense	probably damaging	1.00
R1413:Vmn2r58	UTSW	7	41,513,387 (GRCm39)	missense	probably benign	0.01
R1678:Vmn2r58	UTSW	7	41,513,480 (GRCm39)	missense	probably benign	0.40
R1691:Vmn2r58	UTSW	7	41,486,913 (GRCm39)	missense	possibly damaging	0.95
R1699:Vmn2r58	UTSW	7	41,509,951 (GRCm39)	missense	probably benign
R1865:Vmn2r58	UTSW	7	41,486,682 (GRCm39)	missense	possibly damaging	0.95
R2008:Vmn2r58	UTSW	7	41,509,924 (GRCm39)	missense	probably damaging	1.00
R2036:Vmn2r58	UTSW	7	41,513,417 (GRCm39)	missense	probably benign
R2202:Vmn2r58	UTSW	7	41,513,594 (GRCm39)	missense	probably benign	0.07
R3787:Vmn2r58	UTSW	7	41,513,498 (GRCm39)	missense	probably benign	0.01
R3883:Vmn2r58	UTSW	7	41,513,914 (GRCm39)	nonsense	probably null
R3944:Vmn2r58	UTSW	7	41,513,885 (GRCm39)	missense	probably benign	0.03
R3949:Vmn2r58	UTSW	7	41,513,348 (GRCm39)	missense	probably benign	0.08
R4232:Vmn2r58	UTSW	7	41,487,011 (GRCm39)	missense	possibly damaging	0.91
R4409:Vmn2r58	UTSW	7	41,522,051 (GRCm39)	missense	possibly damaging	0.69
R4411:Vmn2r58	UTSW	7	41,511,360 (GRCm39)	missense	possibly damaging	0.85
R4413:Vmn2r58	UTSW	7	41,511,360 (GRCm39)	missense	possibly damaging	0.85
R4600:Vmn2r58	UTSW	7	41,522,046 (GRCm39)	missense	probably benign	0.03
R4610:Vmn2r58	UTSW	7	41,487,117 (GRCm39)	missense	probably benign
R4646:Vmn2r58	UTSW	7	41,509,935 (GRCm39)	missense	probably damaging	0.96
R4793:Vmn2r58	UTSW	7	41,514,495 (GRCm39)	missense	probably damaging	0.99
R4870:Vmn2r58	UTSW	7	41,486,639 (GRCm39)	missense	possibly damaging	0.76
R4981:Vmn2r58	UTSW	7	41,486,885 (GRCm39)	missense	probably damaging	1.00
R4993:Vmn2r58	UTSW	7	41,487,176 (GRCm39)	missense	probably benign	0.00
R5024:Vmn2r58	UTSW	7	41,513,746 (GRCm39)	missense	probably damaging	0.99
R5064:Vmn2r58	UTSW	7	41,486,534 (GRCm39)	missense	probably damaging	0.99
R5330:Vmn2r58	UTSW	7	41,513,384 (GRCm39)	nonsense	probably null
R5526:Vmn2r58	UTSW	7	41,522,069 (GRCm39)	missense	probably benign	0.01
R5980:Vmn2r58	UTSW	7	41,514,480 (GRCm39)	missense	possibly damaging	0.81
R6163:Vmn2r58	UTSW	7	41,486,825 (GRCm39)	missense	probably benign	0.31
R6365:Vmn2r58	UTSW	7	41,513,607 (GRCm39)	missense	probably benign	0.42
R6567:Vmn2r58	UTSW	7	41,514,673 (GRCm39)	missense	probably benign	0.34
R6594:Vmn2r58	UTSW	7	41,486,535 (GRCm39)	missense	possibly damaging	0.69
R6980:Vmn2r58	UTSW	7	41,513,662 (GRCm39)	missense	possibly damaging	0.64
R7373:Vmn2r58	UTSW	7	41,487,212 (GRCm39)	missense	probably damaging	1.00
R7458:Vmn2r58	UTSW	7	41,487,123 (GRCm39)	missense	probably benign	0.06
R7630:Vmn2r58	UTSW	7	41,513,611 (GRCm39)	missense	probably damaging	0.99
R7807:Vmn2r58	UTSW	7	41,521,910 (GRCm39)	missense	probably benign	0.05
R8114:Vmn2r58	UTSW	7	41,511,392 (GRCm39)	missense	probably damaging	1.00
R8232:Vmn2r58	UTSW	7	41,514,076 (GRCm39)	missense	probably damaging	0.97
R8313:Vmn2r58	UTSW	7	41,521,952 (GRCm39)	missense	probably benign	0.01
R8412:Vmn2r58	UTSW	7	41,513,722 (GRCm39)	missense	probably benign	0.01
R8530:Vmn2r58	UTSW	7	41,513,576 (GRCm39)	missense	probably damaging	1.00
R8851:Vmn2r58	UTSW	7	41,487,219 (GRCm39)	missense	probably benign
R8881:Vmn2r58	UTSW	7	41,486,609 (GRCm39)	missense	probably benign	0.05
R8936:Vmn2r58	UTSW	7	41,513,981 (GRCm39)	missense
R9045:Vmn2r58	UTSW	7	41,487,087 (GRCm39)	missense	probably benign	0.00
R9166:Vmn2r58	UTSW	7	41,513,431 (GRCm39)	missense	probably damaging	1.00
R9706:Vmn2r58	UTSW	7	41,510,000 (GRCm39)	missense	probably damaging	0.99
RF006:Vmn2r58	UTSW	7	41,486,383 (GRCm39)	frame shift	probably null
RF027:Vmn2r58	UTSW	7	41,486,383 (GRCm39)	frame shift	probably null
Z1176:Vmn2r58	UTSW	7	41,513,789 (GRCm39)	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- TCCCAGTACAGTGTGGAAGGCAAC -3'
(R):5'- CGTCAAGGCCAATAACAGGACTCTC -3'

Sequencing Primer
(F):5'- AACTGTTGAACCCATGTTGC -3'
(R):5'- AGGACTCTCAGTTACATCCTGC -3'

Posted On

2014-03-14