Mutagenetix > Incidental Mutation

Incidental Mutation 'R1423:Vmn2r15'

161165

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r15

Ensembl Gene

ENSMUSG00000091375

Gene Name

vomeronasal 2, receptor 15

Synonyms

EG211223

MMRRC Submission

039479-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.090) question?

Stock #

R1423 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

109434135-109445422 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 109441093 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Cysteine at position 255 (Y255C)

Ref Sequence

ENSEMBL: ENSMUSP00000128333 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000167133]

AlphaFold

L7N2A0

Predicted Effect

probably damaging
Transcript: ENSMUST00000167133
AA Change: Y255C

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000128333
Gene: ENSMUSG00000091375
AA Change: Y255C

Domain	Start	End	E-Value	Type
signal peptide	1	24	N/A	INTRINSIC
Pfam:ANF_receptor	75	472	1e-29	PFAM
Pfam:NCD3G	514	568	5.8e-18	PFAM
Pfam:7tm_3	601	836	9.1e-55	PFAM

Meta Mutation Damage Score

0.6467

Coding Region Coverage

1x: 98.9%
3x: 97.9%
10x: 95.0%
20x: 88.8%

Validation Efficiency

100% (48/48)

Allele List at MGI

Other mutations in this stock

Total: 46 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ankrd13d	A	G	19: 4,331,097 (GRCm39)	S64P	probably damaging	Het
Armh4	T	C	14: 49,988,896 (GRCm39)	E691G	probably damaging	Het
Bag4	T	C	8: 26,258,302 (GRCm39)	S342G	probably damaging	Het
Bbs1	G	A	19: 4,944,291 (GRCm39)	T446I	probably benign	Het
Bmyc	A	G	2: 25,597,236 (GRCm39)	D100G	probably damaging	Het
Btbd7	T	C	12: 102,751,734 (GRCm39)	D1010G	possibly damaging	Het
Celsr3	A	T	9: 108,704,104 (GRCm39)	T196S	probably benign	Het
Crtam	C	A	9: 40,884,918 (GRCm39)	R161L	probably benign	Het
Cyp11b2	C	A	15: 74,724,979 (GRCm39)	G290V	probably damaging	Het
Edc4	C	T	8: 106,617,843 (GRCm39)		probably benign	Het
Exd1	A	T	2: 119,370,494 (GRCm39)		probably benign	Het
Fam120a	G	T	13: 49,039,219 (GRCm39)	A979E	possibly damaging	Het
Fbxo16	T	C	14: 65,524,623 (GRCm39)		probably benign	Het
Fbxo44	C	G	4: 148,240,726 (GRCm39)	R220S	probably damaging	Het
Gabrb2	A	G	11: 42,420,298 (GRCm39)	N173S	probably damaging	Het
Gm13741	A	T	2: 87,486,674 (GRCm39)	I197K	possibly damaging	Het
Helb	T	A	10: 119,944,871 (GRCm39)	I222F	probably damaging	Het
Hesx1	A	T	14: 26,723,876 (GRCm39)	Q153L	probably null	Het
Isca1	T	C	13: 59,910,593 (GRCm39)	N33S	probably benign	Het
Itsn2	T	C	12: 4,723,572 (GRCm39)	V1142A	probably damaging	Het
Lama5	T	G	2: 179,837,434 (GRCm39)	T984P	probably damaging	Het
Lima1	T	A	15: 99,717,626 (GRCm39)	K127*	probably null	Het
Lmbrd1	T	A	1: 24,785,959 (GRCm39)	V418D	probably damaging	Het
Mertk	T	C	2: 128,620,883 (GRCm39)	V575A	probably damaging	Het
Mrps28	T	C	3: 8,965,184 (GRCm39)	H85R	probably benign	Het
Mrtfb	T	C	16: 13,230,105 (GRCm39)	V930A	possibly damaging	Het
Naip2	G	A	13: 100,291,386 (GRCm39)	T1184M	probably benign	Het
Naip2	C	T	13: 100,298,368 (GRCm39)	G556D	probably benign	Het
Naip2	TCCC	TCC	13: 100,291,355 (GRCm39)		probably benign	Het
Naip2	G	A	13: 100,291,380 (GRCm39)	S1186F	possibly damaging	Het
Nhlrc3	A	T	3: 53,369,836 (GRCm39)	L45H	probably damaging	Het
Or52e5	T	G	7: 104,719,226 (GRCm39)	M184R	probably damaging	Het
Or52u1	C	T	7: 104,237,682 (GRCm39)	R224*	probably null	Het
Or8s16	T	C	15: 98,211,324 (GRCm39)	T36A	probably damaging	Het
Parp14	G	A	16: 35,677,130 (GRCm39)	A946V	probably benign	Het
Pcdhb15	A	T	18: 37,606,975 (GRCm39)	D69V	probably damaging	Het
Pitpnm1	G	A	19: 4,162,392 (GRCm39)	R1074H	probably damaging	Het
Plb1	A	G	5: 32,450,601 (GRCm39)	N381D	probably benign	Het
Poc1b	T	C	10: 98,988,725 (GRCm39)	S247P	probably damaging	Het
Prl7b1	T	A	13: 27,786,110 (GRCm39)	N186I	probably damaging	Het
Riok1	T	C	13: 38,233,090 (GRCm39)	M241T	probably damaging	Het
Tigit	T	C	16: 43,469,395 (GRCm39)	E232G	probably benign	Het
Tpst2	T	A	5: 112,455,488 (GRCm39)	L9Q	probably benign	Het
Ttbk1	T	C	17: 46,757,080 (GRCm39)		probably benign	Het
Vmn1r3	A	T	4: 3,185,231 (GRCm39)	N25K	probably damaging	Het
Vmn2r121	T	A	X: 123,039,602 (GRCm39)	H522L	possibly damaging	Het

Other mutations in Vmn2r15

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01335:Vmn2r15	APN	5	109,434,602 (GRCm39)	missense	possibly damaging	0.70
IGL01367:Vmn2r15	APN	5	109,441,075 (GRCm39)	missense	probably damaging	0.99
IGL01844:Vmn2r15	APN	5	109,434,135 (GRCm39)	makesense	probably null
IGL02190:Vmn2r15	APN	5	109,441,240 (GRCm39)	missense	probably damaging	1.00
IGL02754:Vmn2r15	APN	5	109,441,134 (GRCm39)	nonsense	probably null
IGL02797:Vmn2r15	APN	5	109,445,250 (GRCm39)	missense	probably benign	0.18
IGL03301:Vmn2r15	APN	5	109,445,221 (GRCm39)	critical splice donor site	probably null
IGL03407:Vmn2r15	APN	5	109,434,185 (GRCm39)	nonsense	probably null
BB001:Vmn2r15	UTSW	5	109,434,254 (GRCm39)	missense	probably damaging	1.00
BB011:Vmn2r15	UTSW	5	109,434,254 (GRCm39)	missense	probably damaging	1.00
PIT4445001:Vmn2r15	UTSW	5	109,435,008 (GRCm39)	missense	probably damaging	0.99
PIT4520001:Vmn2r15	UTSW	5	109,434,871 (GRCm39)	missense	probably damaging	1.00
R0038:Vmn2r15	UTSW	5	109,441,010 (GRCm39)	missense	possibly damaging	0.46
R0111:Vmn2r15	UTSW	5	109,435,022 (GRCm39)	missense	possibly damaging	0.56
R0379:Vmn2r15	UTSW	5	109,434,344 (GRCm39)	missense	probably damaging	1.00
R0427:Vmn2r15	UTSW	5	109,434,953 (GRCm39)	missense	probably damaging	1.00
R0639:Vmn2r15	UTSW	5	109,440,881 (GRCm39)	missense	probably benign	0.22
R0964:Vmn2r15	UTSW	5	109,445,401 (GRCm39)	missense	probably benign	0.34
R1147:Vmn2r15	UTSW	5	109,441,072 (GRCm39)	missense	probably damaging	1.00
R1147:Vmn2r15	UTSW	5	109,441,072 (GRCm39)	missense	probably damaging	1.00
R1232:Vmn2r15	UTSW	5	109,441,168 (GRCm39)	missense	probably benign	0.39
R1241:Vmn2r15	UTSW	5	109,440,770 (GRCm39)	missense	probably damaging	1.00
R1244:Vmn2r15	UTSW	5	109,441,092 (GRCm39)	nonsense	probably null
R1394:Vmn2r15	UTSW	5	109,442,014 (GRCm39)	missense	probably benign	0.44
R1395:Vmn2r15	UTSW	5	109,442,014 (GRCm39)	missense	probably benign	0.44
R1439:Vmn2r15	UTSW	5	109,441,953 (GRCm39)	missense	probably damaging	1.00
R1513:Vmn2r15	UTSW	5	109,441,195 (GRCm39)	missense	probably damaging	1.00
R1777:Vmn2r15	UTSW	5	109,442,136 (GRCm39)	missense	possibly damaging	0.79
R1844:Vmn2r15	UTSW	5	109,434,860 (GRCm39)	nonsense	probably null
R2072:Vmn2r15	UTSW	5	109,434,619 (GRCm39)	missense	possibly damaging	0.65
R2074:Vmn2r15	UTSW	5	109,434,619 (GRCm39)	missense	possibly damaging	0.65
R2122:Vmn2r15	UTSW	5	109,434,322 (GRCm39)	missense	probably damaging	1.00
R2208:Vmn2r15	UTSW	5	109,445,309 (GRCm39)	missense	possibly damaging	0.64
R2268:Vmn2r15	UTSW	5	109,441,073 (GRCm39)	missense	probably benign	0.31
R2831:Vmn2r15	UTSW	5	109,434,458 (GRCm39)	missense	probably damaging	1.00
R3848:Vmn2r15	UTSW	5	109,445,312 (GRCm39)	missense	probably benign	0.00
R4058:Vmn2r15	UTSW	5	109,441,312 (GRCm39)	missense	probably damaging	0.99
R4615:Vmn2r15	UTSW	5	109,441,348 (GRCm39)	missense	possibly damaging	0.91
R4663:Vmn2r15	UTSW	5	109,441,940 (GRCm39)	missense	probably benign
R4681:Vmn2r15	UTSW	5	109,434,488 (GRCm39)	missense	probably damaging	0.97
R4751:Vmn2r15	UTSW	5	109,434,620 (GRCm39)	missense	probably benign	0.01
R5095:Vmn2r15	UTSW	5	109,436,317 (GRCm39)	critical splice acceptor site	probably null
R5300:Vmn2r15	UTSW	5	109,441,974 (GRCm39)	missense	probably damaging	0.99
R5309:Vmn2r15	UTSW	5	109,440,956 (GRCm39)	missense	probably damaging	0.99
R5335:Vmn2r15	UTSW	5	109,434,673 (GRCm39)	missense	probably damaging	0.99
R5421:Vmn2r15	UTSW	5	109,434,401 (GRCm39)	missense	probably damaging	1.00
R5805:Vmn2r15	UTSW	5	109,434,806 (GRCm39)	missense	possibly damaging	0.88
R6280:Vmn2r15	UTSW	5	109,441,291 (GRCm39)	missense	possibly damaging	0.65
R6324:Vmn2r15	UTSW	5	109,434,137 (GRCm39)	makesense	probably null
R6383:Vmn2r15	UTSW	5	109,441,092 (GRCm39)	nonsense	probably null
R6772:Vmn2r15	UTSW	5	109,434,238 (GRCm39)	missense	probably damaging	0.99
R6991:Vmn2r15	UTSW	5	109,441,180 (GRCm39)	missense	probably damaging	1.00
R7194:Vmn2r15	UTSW	5	109,440,649 (GRCm39)	missense	probably damaging	1.00
R7365:Vmn2r15	UTSW	5	109,445,388 (GRCm39)	missense	probably benign	0.15
R7365:Vmn2r15	UTSW	5	109,441,105 (GRCm39)	missense	probably benign	0.19
R7423:Vmn2r15	UTSW	5	109,445,394 (GRCm39)	missense	probably benign	0.00
R7552:Vmn2r15	UTSW	5	109,440,774 (GRCm39)	nonsense	probably null
R7619:Vmn2r15	UTSW	5	109,436,190 (GRCm39)	critical splice donor site	probably null
R7892:Vmn2r15	UTSW	5	109,434,217 (GRCm39)	missense	probably damaging	1.00
R7924:Vmn2r15	UTSW	5	109,434,254 (GRCm39)	missense	probably damaging	1.00
R8058:Vmn2r15	UTSW	5	109,440,956 (GRCm39)	missense	probably damaging	0.99
R8099:Vmn2r15	UTSW	5	109,441,185 (GRCm39)	missense	possibly damaging	0.58
R8189:Vmn2r15	UTSW	5	109,434,713 (GRCm39)	missense	probably benign	0.02
R8275:Vmn2r15	UTSW	5	109,434,150 (GRCm39)	missense	probably benign	0.08
R8277:Vmn2r15	UTSW	5	109,441,310 (GRCm39)	missense	probably damaging	1.00
R8277:Vmn2r15	UTSW	5	109,440,970 (GRCm39)	missense	probably benign	0.00
R8465:Vmn2r15	UTSW	5	109,445,302 (GRCm39)	missense	probably damaging	1.00
R8679:Vmn2r15	UTSW	5	109,434,779 (GRCm39)	missense	probably benign	0.31
R8682:Vmn2r15	UTSW	5	109,441,938 (GRCm39)	missense	probably damaging	0.98
R8809:Vmn2r15	UTSW	5	109,434,874 (GRCm39)	missense	probably benign	0.22
R9016:Vmn2r15	UTSW	5	109,442,109 (GRCm39)	missense	probably benign	0.01
R9372:Vmn2r15	UTSW	5	109,441,953 (GRCm39)	missense	possibly damaging	0.60
R9596:Vmn2r15	UTSW	5	109,440,791 (GRCm39)	missense	probably benign	0.01
R9619:Vmn2r15	UTSW	5	109,440,622 (GRCm39)	missense	possibly damaging	0.58
R9685:Vmn2r15	UTSW	5	109,440,598 (GRCm39)	missense	probably benign	0.05
R9716:Vmn2r15	UTSW	5	109,445,224 (GRCm39)	missense	possibly damaging	0.88
R9772:Vmn2r15	UTSW	5	109,434,923 (GRCm39)	missense	probably damaging	1.00
X0065:Vmn2r15	UTSW	5	109,441,174 (GRCm39)	missense	probably damaging	1.00
Z1187:Vmn2r15	UTSW	5	109,434,529 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GGTGTGCAAAAGTGACAGTTCCG -3'
(R):5'- CCTGAGTGACCATGACCAGTTTCC -3'

Sequencing Primer
(F):5'- TGACAGTTCCGTGAAAGAAATC -3'
(R):5'- TATGTCCATCAGGTAGCCATCAAG -3'

Posted On

2014-03-14