Mutagenetix > Incidental Mutation

Incidental Mutation 'R1428:Ifi27l2a'

161405

Institutional Source

Beutler Lab

Gene Symbol

Ifi27l2a

Ensembl Gene

ENSMUSG00000079017

Gene Name

interferon, alpha-inducible protein 27 like 2A

Synonyms

Ifi27, 2310061N23Rik

MMRRC Submission

039484-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R1428 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

103408426-103409939 bp(-) (GRCm39)

Type of Mutation

unclassified

DNA Base Change (assembly)

G to T at 103409093 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000082142 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000055071] [ENSMUST00000066701] [ENSMUST00000076702] [ENSMUST00000076788] [ENSMUST00000079294] [ENSMUST00000085065]

AlphaFold

Q8R412

Predicted Effect

unknown
Transcript: ENSMUST00000055071
AA Change: A66E

SMART Domains

Protein: ENSMUSP00000054698
Gene: ENSMUSG00000079017
AA Change: A66E

Domain	Start	End	E-Value	Type
Pfam:Ifi-6-16	10	88	4.1e-31	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000066701

SMART Domains

Protein: ENSMUSP00000068729
Gene: ENSMUSG00000064215

Domain	Start	End	E-Value	Type
transmembrane domain	21	43	N/A	INTRINSIC
transmembrane domain	53	75	N/A	INTRINSIC
Pfam:Ifi-6-16	85	167	2.6e-33	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000076702

SMART Domains

Protein: ENSMUSP00000075994
Gene: ENSMUSG00000064215

Domain	Start	End	E-Value	Type
low complexity region	43	59	N/A	INTRINSIC
Pfam:Ifi-6-16	73	157	7.1e-32	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000076788

SMART Domains

Protein: ENSMUSP00000076068
Gene: ENSMUSG00000064215

Domain	Start	End	E-Value	Type
Pfam:Ifi-6-16	35	117	7.2e-32	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000079294

SMART Domains

Protein: ENSMUSP00000078275
Gene: ENSMUSG00000064215

Domain	Start	End	E-Value	Type
low complexity region	43	59	N/A	INTRINSIC
Pfam:Ifi-6-16	122	202	8e-33	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000085065

SMART Domains

Protein: ENSMUSP00000082142
Gene: ENSMUSG00000064215

Domain	Start	End	E-Value	Type
low complexity region	43	59	N/A	INTRINSIC
Pfam:Ifi-6-16	132	210	1.8e-29	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000153670

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.0%
3x: 98.0%
10x: 95.5%
20x: 90.2%

Validation Efficiency

98% (56/57)

MGI Phenotype

PHENOTYPE: Mice homozygous for a knock-out allele exhibit normal susceptibility to infection with H1N1 or H7N7 influenza A virus, although sloughing of dead cells into bronchiolar lumen persists somewhat longer after infection than in infected controls. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 49 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700034J05Rik	T	C	6: 146,853,909 (GRCm39)	I249M	possibly damaging	Het
Abl1	G	T	2: 31,691,822 (GRCm39)	A1114S	probably damaging	Het
Acbd5	T	C	2: 22,989,733 (GRCm39)	V452A	probably damaging	Het
Armc12	A	G	17: 28,756,910 (GRCm39)	D225G	probably damaging	Het
Atad3a	T	A	4: 155,840,139 (GRCm39)	Q121H	probably damaging	Het
Atosa	A	T	9: 74,913,603 (GRCm39)	T79S	probably benign	Het
Bptf	T	A	11: 106,963,873 (GRCm39)	I1711F	probably damaging	Het
C2cd4c	A	T	10: 79,448,064 (GRCm39)	I361N	probably damaging	Het
Canx	A	G	11: 50,199,221 (GRCm39)		probably benign	Het
Ccdc127	C	T	13: 74,505,034 (GRCm39)	T194I	probably benign	Het
Cdc42ep3	T	C	17: 79,642,465 (GRCm39)	K152E	probably benign	Het
Cdh15	G	C	8: 123,584,234 (GRCm39)	E112Q	probably damaging	Het
Cnbd2	T	C	2: 156,181,204 (GRCm39)		probably null	Het
Cplane1	T	A	15: 8,248,853 (GRCm39)	Y1801N	possibly damaging	Het
Crybg1	T	C	10: 43,851,074 (GRCm39)	N1599S	probably benign	Het
Cyc1	T	C	15: 76,228,548 (GRCm39)	V59A	probably benign	Het
Cyp2j11	T	C	4: 96,183,117 (GRCm39)	K484E	probably benign	Het
Ddx60	T	C	8: 62,411,193 (GRCm39)		probably benign	Het
Epg5	T	C	18: 78,005,642 (GRCm39)	S711P	probably damaging	Het
Espl1	A	T	15: 102,214,120 (GRCm39)	Q649L	probably benign	Het
Eya1	G	A	1: 14,374,638 (GRCm39)		probably benign	Het
Fat2	T	A	11: 55,186,913 (GRCm39)	Y1311F	probably damaging	Het
Garin5b	T	A	7: 4,760,687 (GRCm39)	H675L	possibly damaging	Het
Gins4	T	C	8: 23,717,144 (GRCm39)	Y208C	probably damaging	Het
Gsk3b	T	C	16: 37,910,937 (GRCm39)	V17A	probably benign	Het
Gykl1	A	T	18: 52,827,833 (GRCm39)	K347I	probably benign	Het
Helz	T	A	11: 107,483,666 (GRCm39)		probably benign	Het
Hivep3	C	T	4: 119,953,772 (GRCm39)	T696I	possibly damaging	Het
Kif13a	A	G	13: 46,944,987 (GRCm39)		probably benign	Het
Kpna3	C	T	14: 61,620,669 (GRCm39)		probably benign	Het
Mmp15	C	G	8: 96,096,190 (GRCm39)	P327R	probably benign	Het
Mrc1	A	T	2: 14,320,074 (GRCm39)	T1003S	probably benign	Het
Mtss1	T	C	15: 58,819,239 (GRCm39)	D393G	probably benign	Het
Olfm4	T	A	14: 80,258,843 (GRCm39)	Y331N	probably damaging	Het
Or10a49	T	A	7: 108,468,167 (GRCm39)	N65Y	probably damaging	Het
Or4c12b	G	C	2: 89,646,725 (GRCm39)	L12F	probably damaging	Het
P2rx3	G	C	2: 84,855,294 (GRCm39)	T54R	possibly damaging	Het
Pacsin1	C	T	17: 27,924,937 (GRCm39)	T217I	probably damaging	Het
Phlpp1	A	G	1: 106,308,155 (GRCm39)		probably null	Het
Pknox1	T	C	17: 31,811,066 (GRCm39)		probably benign	Het
Plb1	A	G	5: 32,422,256 (GRCm39)	R70G	possibly damaging	Het
Rab3gap2	G	A	1: 184,980,101 (GRCm39)	A340T	probably damaging	Het
Rnf103	T	A	6: 71,485,983 (GRCm39)	W205R	probably damaging	Het
Rps6kc1	G	A	1: 190,530,923 (GRCm39)	T936M	probably damaging	Het
Spef2	T	C	15: 9,596,793 (GRCm39)		probably benign	Het
Sstr4	A	G	2: 148,238,279 (GRCm39)	S297G	probably benign	Het
Timm8a1	C	T	X: 133,438,872 (GRCm39)	E93K	probably benign	Het
Uck1	A	C	2: 32,148,367 (GRCm39)	Y150D	probably damaging	Het
Yipf4	A	G	17: 74,805,300 (GRCm39)		probably benign	Het

Other mutations in Ifi27l2a

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01114:Ifi27l2a	APN	12	103,403,792 (GRCm39)	unclassified	probably benign
IGL01872:Ifi27l2a	APN	12	103,401,719 (GRCm39)	missense	probably damaging	0.99
IGL03061:Ifi27l2a	APN	12	103,401,803 (GRCm39)	missense	possibly damaging	0.85
IGL03402:Ifi27l2a	APN	12	103,405,772 (GRCm39)	missense	probably damaging	1.00
R0924:Ifi27l2a	UTSW	12	103,408,639 (GRCm39)	missense	probably damaging	0.99
R1712:Ifi27l2a	UTSW	12	103,406,202 (GRCm39)	splice site	probably null
R1763:Ifi27l2a	UTSW	12	103,403,941 (GRCm39)	missense	possibly damaging	0.80
R6883:Ifi27l2a	UTSW	12	103,409,756 (GRCm39)	unclassified	probably benign
R8325:Ifi27l2a	UTSW	12	103,409,144 (GRCm39)	missense	unknown
R8868:Ifi27l2a	UTSW	12	103,402,899 (GRCm39)	missense	possibly damaging	0.93
R9149:Ifi27l2a	UTSW	12	103,405,678 (GRCm39)	missense	possibly damaging	0.92

Predicted Primers

PCR Primer
(F):5'- AGTGACCTGATGCTCAGAGATGCC -3'
(R):5'- GTCCCCAGAAACAGTCAGGGAATTG -3'

Sequencing Primer
(F):5'- GAGATGCTCTCTCTATAGCAGGAC -3'
(R):5'- GTGGGTCATTTCCCTGAGCC -3'

Posted On

2014-03-14