Mutagenetix > Incidental Mutations

Incidental Mutation 'R1426:Zyg11b'

162242

Institutional Source

Beutler Lab

Gene Symbol

Zyg11b

Ensembl Gene

ENSMUSG00000034636

Gene Name

zyg-ll family member B, cell cycle regulator

Synonyms

1110046I03Rik, LOC242610, 2810482G21Rik, D4Mgi23

MMRRC Submission

039482-MU

Accession Numbers

Is this an essential gene?

Possibly non essential (E-score: 0.465) question?

Stock #

R1426 (G1)

Quality Score

204

Status

Validated

Chromosome

Chromosomal Location

108229724-108301096 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 108250812 bp

Zygosity

Heterozygous

Amino Acid Change

Arginine to Cysteine at position 466 (R466C)

Ref Sequence

ENSEMBL: ENSMUSP00000043844 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000043616]

Predicted Effect

probably damaging
Transcript: ENSMUST00000043616
AA Change: R466C

PolyPhen 2 Score 0.996 (Sensitivity: 0.55; Specificity: 0.98)

SMART Domains

Protein: ENSMUSP00000043844
Gene: ENSMUSG00000034636
AA Change: R466C

Domain	Start	End	E-Value	Type
SCOP:d1jdha_	370	722	3e-16	SMART
Blast:ARM	480	526	1e-17	BLAST
Blast:ARM	528	570	3e-19	BLAST
Blast:ARM	638	679	3e-10	BLAST

Meta Mutation Damage Score

0.6467

Coding Region Coverage

1x: 98.8%
3x: 97.7%
10x: 94.3%
20x: 86.1%

Validation Efficiency

100% (40/40)

Allele List at MGI

Other mutations in this stock

Total: 38 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcc10	A	T	17: 46,324,435	V214E	probably damaging	Het
Adh1	A	G	3: 138,286,795	D224G	probably damaging	Het
Arhgap28	C	A	17: 67,857,464	Q554H	probably damaging	Het
Atp8a2	T	C	14: 59,860,270	K770E	probably benign	Het
Brat1	G	A	5: 140,718,013	V674I	probably benign	Het
Brd2	ATCTTCTTC	ATCTTC	17: 34,114,007		probably benign	Het
Ccdc162	T	C	10: 41,553,182	D438G	possibly damaging	Het
Cyp4x1	T	A	4: 115,112,791		probably benign	Het
Dip2a	T	C	10: 76,279,820		probably benign	Het
Eif2s1	A	G	12: 78,881,168	D206G	probably benign	Het
Elovl7	T	A	13: 108,282,494	I220N	possibly damaging	Het
Gsto1	A	G	19: 47,857,942	E76G	probably damaging	Het
Hspa14	A	T	2: 3,508,821	W12R	probably damaging	Het
L3mbtl2	T	A	15: 81,676,317	C260S	possibly damaging	Het
Lama3	G	T	18: 12,481,098		probably null	Het
Lrrc34	T	A	3: 30,643,579		probably benign	Het
Lrrc45	A	T	11: 120,720,013	Q525L	probably benign	Het
Lss	T	C	10: 76,536,303	I164T	probably damaging	Het
Myh11	T	A	16: 14,205,931	K1527*	probably null	Het
Naip2	T	C	13: 100,161,854	E558G	probably benign	Het
Naip2	C	T	13: 100,161,860	G556D	probably benign	Het
Ncoa1	T	A	12: 4,270,737		probably benign	Het
Olfr262	G	T	19: 12,241,182	Q160K	possibly damaging	Het
Olfr768	A	T	10: 129,093,690	C95S	probably damaging	Het
Pafah1b3	T	C	7: 25,297,135	E41G	possibly damaging	Het
Pnmal1	C	T	7: 16,960,984	P255S	possibly damaging	Het
Prkar2b	A	T	12: 31,962,988		probably benign	Het
Rbck1	A	T	2: 152,327,241		probably benign	Het
Rcor2	A	G	19: 7,271,030	S137G	possibly damaging	Het
Slc25a48	T	A	13: 56,448,991		probably benign	Het
Slc7a4	A	G	16: 17,573,944		probably null	Het
Tert	T	C	13: 73,642,353		probably benign	Het
Traf7	A	T	17: 24,511,681	I344N	probably damaging	Het
Vmn1r194	T	A	13: 22,245,066	F284L	probably damaging	Het
Xpc	A	G	6: 91,493,238	M699T	probably damaging	Het
Zbtb5	T	C	4: 44,993,968	H472R	possibly damaging	Het
Zfp786	A	G	6: 47,825,079	V88A	probably benign	Het
Zkscan7	T	C	9: 122,895,163	I399T	probably benign	Het

Other mutations in Zyg11b

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01080:Zyg11b	APN	4	108237416	missense	probably damaging	1.00
IGL01143:Zyg11b	APN	4	108244994	missense	possibly damaging	0.69
IGL01627:Zyg11b	APN	4	108250788	missense	probably benign	0.19
IGL02517:Zyg11b	APN	4	108266318	missense	probably damaging	0.96
IGL03166:Zyg11b	APN	4	108265889	missense	probably benign	0.32
arch	UTSW	4	108237380	nonsense	probably null
R0326:Zyg11b	UTSW	4	108272253	missense	possibly damaging	0.77
R0345:Zyg11b	UTSW	4	108266407	missense	probably damaging	1.00
R0396:Zyg11b	UTSW	4	108255308	missense	probably damaging	1.00
R0571:Zyg11b	UTSW	4	108260042	missense	probably damaging	1.00
R0718:Zyg11b	UTSW	4	108242076	missense	possibly damaging	0.94
R1495:Zyg11b	UTSW	4	108266213	missense	probably damaging	1.00
R1829:Zyg11b	UTSW	4	108266093	missense	possibly damaging	0.65
R1907:Zyg11b	UTSW	4	108255226	missense	probably damaging	1.00
R1916:Zyg11b	UTSW	4	108272283	missense	probably damaging	0.99
R1980:Zyg11b	UTSW	4	108265930	missense	probably damaging	0.99
R2070:Zyg11b	UTSW	4	108250819	missense	possibly damaging	0.73
R2495:Zyg11b	UTSW	4	108244724	critical splice donor site	probably null
R4717:Zyg11b	UTSW	4	108241872	missense	probably damaging	0.99
R5889:Zyg11b	UTSW	4	108237380	nonsense	probably null
R5957:Zyg11b	UTSW	4	108245013	missense	probably damaging	1.00
R6606:Zyg11b	UTSW	4	108236089	missense	probably benign	0.00
R7151:Zyg11b	UTSW	4	108244922	missense	possibly damaging	0.49
R7316:Zyg11b	UTSW	4	108250502	missense	possibly damaging	0.81
R7488:Zyg11b	UTSW	4	108266458	missense	possibly damaging	0.65
X0022:Zyg11b	UTSW	4	108236101	missense	probably benign
X0067:Zyg11b	UTSW	4	108255346	missense	probably benign
Z1177:Zyg11b	UTSW	4	108255364	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CAAGGGCCAGTAAACTTACCCTGAC -3'
(R):5'- GTCCCAAGTTGCACCATCGACC -3'

Sequencing Primer
(F):5'- TGAGTTTACTACAGACTGCCG -3'
(R):5'- CCACATCTGGCTCCCTGAAG -3'

Posted On

2014-03-14