Mutagenetix > Incidental Mutation

Incidental Mutation 'R1396:Wrn'

162647

Institutional Source

Beutler Lab

Gene Symbol

Wrn

Ensembl Gene

ENSMUSG00000031583

Gene Name

Werner syndrome RecQ like helicase

Synonyms

MMRRC Submission

039458-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.381) question?

Stock #

R1396 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

33234384-33385527 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 33268819 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Glycine to Aspartic acid at position 769 (G769D)

Ref Sequence

ENSEMBL: ENSMUSP00000147379 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000033990] [ENSMUST00000033991] [ENSMUST00000211498]

AlphaFold

O09053

Predicted Effect

probably damaging
Transcript: ENSMUST00000033990
AA Change: G1012D

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000033990
Gene: ENSMUSG00000031583
AA Change: G1012D

Domain	Start	End	E-Value	Type
35EXOc	47	226	1e-47	SMART
low complexity region	484	489	N/A	INTRINSIC
DEXDc	509	704	2.3e-28	SMART
HELICc	743	824	3.7e-27	SMART
RQC	923	1028	3.1e-28	SMART
HRDC	1115	1194	1.5e-26	SMART
low complexity region	1205	1216	N/A	INTRINSIC
Pfam:HTH_40	1222	1318	2.7e-9	PFAM
low complexity region	1342	1356	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000033991
AA Change: G1012D

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000033991
Gene: ENSMUSG00000031583
AA Change: G1012D

Domain	Start	End	E-Value	Type
35EXOc	47	226	1.1e-47	SMART
low complexity region	484	489	N/A	INTRINSIC
DEXDc	509	704	2.4e-28	SMART
HELICc	743	824	3.7e-27	SMART
Pfam:RecQ_Zn_bind	835	905	2.2e-8	PFAM
RQC	923	1028	3.2e-28	SMART
HRDC	1115	1194	1.5e-26	SMART
low complexity region	1205	1216	N/A	INTRINSIC
Pfam:HTH_40	1223	1317	4.3e-10	PFAM
low complexity region	1342	1356	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000211498
AA Change: G769D

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

Coding Region Coverage

1x: 98.9%
3x: 98.0%
10x: 95.3%
20x: 89.4%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the RecQ subfamily and the DEAH (Asp-Glu-Ala-His) subfamily of DNA and RNA helicases. DNA helicases are involved in many aspects of DNA metabolism, including transcription, replication, recombination, and repair. This protein contains a nuclear localization signal in the C-terminus and shows a predominant nucleolar localization. It possesses an intrinsic 3' to 5' DNA helicase activity, and is also a 3' to 5' exonuclease. Based on interactions between this protein and Ku70/80 heterodimer in DNA end processing, this protein may be involved in the repair of double strand DNA breaks. Defects in this gene are the cause of Werner syndrome, an autosomal recessive disorder characterized by premature aging. [provided by RefSeq, Jul 2008]
PHENOTYPE: Homozygous mutants show enhanced frequency and variety of tumors in conjunction with Trp53 knockout alleles. Homozygotes also have an elevated frequency of somatic reversion of the pink-eyed dilution unstable mutation. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 57 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4921530L21Rik	A	G	14: 95,882,551 (GRCm38)	N248S	probably benign	Het
4930486L24Rik	G	A	13: 60,853,243 (GRCm38)	P160S	probably benign	Het
Adamts12	G	T	15: 11,311,472 (GRCm38)	D1272Y	probably benign	Het
Akr1c20	A	T	13: 4,507,727 (GRCm38)	V267D	probably damaging	Het
C1s1	A	G	6: 124,531,051 (GRCm38)	S660P	probably damaging	Het
Ccdc40	A	G	11: 119,231,803 (GRCm38)	T144A	possibly damaging	Het
Cdk20	T	C	13: 64,437,403 (GRCm38)	I167T	probably damaging	Het
Chd6	A	G	2: 160,983,103 (GRCm38)	L1212S	probably damaging	Het
Clock	G	C	5: 76,266,802 (GRCm38)	D15E	probably benign	Het
Clstn2	A	G	9: 97,461,393 (GRCm38)	V667A	probably benign	Het
Cr2	A	T	1: 195,169,253 (GRCm38)		probably null	Het
Cyp2e1	A	G	7: 140,773,079 (GRCm38)	D343G	probably damaging	Het
Dync1h1	G	A	12: 110,636,509 (GRCm38)	E2195K	probably benign	Het
Etf1	G	A	18: 34,908,167 (GRCm38)	T298I	possibly damaging	Het
Gm5431	T	C	11: 48,895,434 (GRCm38)		probably benign	Het
Gss	G	A	2: 155,567,721 (GRCm38)	T265I	probably damaging	Het
Heatr1	C	T	13: 12,406,046 (GRCm38)	S406L	possibly damaging	Het
Hgsnat	C	A	8: 25,957,335 (GRCm38)	M310I	possibly damaging	Het
Inpp5b	G	A	4: 124,789,080 (GRCm38)	R598H	probably damaging	Het
Ints2	G	T	11: 86,249,248 (GRCm38)	Q253K	probably damaging	Het
Kng1	A	G	16: 23,078,980 (GRCm38)	M377V	probably benign	Het
Krt72	C	T	15: 101,786,005 (GRCm38)		probably null	Het
Lemd2	G	C	17: 27,190,732 (GRCm38)	R482G	probably damaging	Het
Lrpprc	C	T	17: 84,726,303 (GRCm38)	D1049N	possibly damaging	Het
Lrrc49	A	T	9: 60,680,527 (GRCm38)	H117Q	probably damaging	Het
Mcm6	A	T	1: 128,351,476 (GRCm38)	F191Y	probably damaging	Het
Mecom	T	C	3: 29,979,800 (GRCm38)	T252A	possibly damaging	Het
Mgat4e	T	C	1: 134,541,533 (GRCm38)	T258A	probably benign	Het
Mpeg1	A	G	19: 12,462,804 (GRCm38)	N542S	probably damaging	Het
Nln	C	T	13: 104,061,753 (GRCm38)	V184I	probably benign	Het
Nova1	A	C	12: 46,816,893 (GRCm38)	F91L	unknown	Het
Polk	T	C	13: 96,484,208 (GRCm38)	I516V	probably benign	Het
Ppig	C	T	2: 69,749,018 (GRCm38)	P357S	unknown	Het
Ptpro	T	A	6: 137,443,594 (GRCm38)	V1007D	probably damaging	Het
Rasal2	A	T	1: 157,164,666 (GRCm38)	H552Q	probably damaging	Het
Rnf41	G	A	10: 128,435,571 (GRCm38)	E117K	probably benign	Het
Sbk3	A	G	7: 4,967,453 (GRCm38)	Y306H	possibly damaging	Het
Senp1	A	G	15: 98,076,554 (GRCm38)	S126P	probably benign	Het
Sfr1	A	G	19: 47,733,690 (GRCm38)	K182E	probably benign	Het
Slc25a4	C	A	8: 46,209,288 (GRCm38)	R111L	probably damaging	Het
Slc9c1	A	T	16: 45,573,347 (GRCm38)	Y551F	probably benign	Het
Stard4	A	T	18: 33,206,210 (GRCm38)	N80K	probably damaging	Het
Tbk1	G	A	10: 121,571,916 (GRCm38)	T104M	probably damaging	Het
Tedc2	T	A	17: 24,216,317 (GRCm38)	E366V	probably damaging	Het
Tedc2	C	A	17: 24,216,318 (GRCm38)	E366*	probably null	Het
Tmem102	A	T	11: 69,804,370 (GRCm38)	W259R	probably damaging	Het
Tnk1	A	T	11: 69,853,136 (GRCm38)	C466S	probably benign	Het
Tspoap1	A	C	11: 87,766,120 (GRCm38)	Q307P	probably damaging	Het
Ugt2b35	A	G	5: 87,011,530 (GRCm38)	N528D	possibly damaging	Het
Usp6nl	A	G	2: 6,426,998 (GRCm38)		probably null	Het
Vmn1r203	C	T	13: 22,524,508 (GRCm38)	T153M	probably benign	Het
Vmn1r89	T	A	7: 13,220,011 (GRCm38)	S157T	probably damaging	Het
Vmn2r103	A	T	17: 19,792,968 (GRCm38)	Y117F	probably benign	Het
Vmn2r116	A	C	17: 23,386,141 (GRCm38)	M143L	probably benign	Het
Vmn2r62	A	T	7: 42,764,837 (GRCm38)	D727E	probably damaging	Het
Vps13c	A	G	9: 67,955,022 (GRCm38)	I2974V	probably benign	Het
Zhx3	T	A	2: 160,781,020 (GRCm38)	H409L	possibly damaging	Het

Other mutations in Wrn

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00659:Wrn	APN	8	33,322,377 (GRCm38)	splice site	probably benign
IGL00661:Wrn	APN	8	33,319,145 (GRCm38)	splice site	probably benign
IGL01472:Wrn	APN	8	33,329,172 (GRCm38)	missense	possibly damaging	0.93
IGL01544:Wrn	APN	8	33,324,526 (GRCm38)	missense	probably benign	0.00
IGL01599:Wrn	APN	8	33,241,011 (GRCm38)	missense	possibly damaging	0.69
IGL01688:Wrn	APN	8	33,310,702 (GRCm38)	splice site	probably benign
IGL01916:Wrn	APN	8	33,257,224 (GRCm38)	missense	possibly damaging	0.78
IGL01925:Wrn	APN	8	33,319,180 (GRCm38)	missense	probably benign	0.42
IGL02068:Wrn	APN	8	33,310,749 (GRCm38)	missense	probably benign	0.38
IGL02084:Wrn	APN	8	33,285,179 (GRCm38)	missense	probably benign
IGL02167:Wrn	APN	8	33,317,555 (GRCm38)	missense	probably damaging	1.00
IGL02230:Wrn	APN	8	33,317,563 (GRCm38)	missense	probably damaging	1.00
IGL02717:Wrn	APN	8	33,343,573 (GRCm38)	missense	probably damaging	1.00
IGL02982:Wrn	APN	8	33,343,066 (GRCm38)	missense	probably damaging	1.00
IGL03030:Wrn	APN	8	33,248,961 (GRCm38)	missense	possibly damaging	0.94
IGL03088:Wrn	APN	8	33,268,823 (GRCm38)	splice site	probably benign
IGL03179:Wrn	APN	8	33,310,706 (GRCm38)	splice site	probably null
IGL03306:Wrn	APN	8	33,336,121 (GRCm38)	missense	probably damaging	1.00
R0004:Wrn	UTSW	8	33,317,560 (GRCm38)	missense	probably damaging	1.00
R0190:Wrn	UTSW	8	33,240,983 (GRCm38)	missense	probably benign	0.02
R0441:Wrn	UTSW	8	33,268,750 (GRCm38)	missense	probably benign	0.24
R0463:Wrn	UTSW	8	33,280,815 (GRCm38)	missense	possibly damaging	0.84
R0538:Wrn	UTSW	8	33,336,091 (GRCm38)	missense	probably damaging	0.99
R0682:Wrn	UTSW	8	33,267,820 (GRCm38)	missense	probably benign	0.00
R0729:Wrn	UTSW	8	33,248,918 (GRCm38)	splice site	probably null
R0744:Wrn	UTSW	8	33,295,006 (GRCm38)	missense	possibly damaging	0.91
R0836:Wrn	UTSW	8	33,295,006 (GRCm38)	missense	possibly damaging	0.91
R1168:Wrn	UTSW	8	33,316,408 (GRCm38)	missense	probably damaging	1.00
R1301:Wrn	UTSW	8	33,292,686 (GRCm38)	missense	probably damaging	1.00
R1352:Wrn	UTSW	8	33,294,916 (GRCm38)	missense	probably benign	0.25
R1432:Wrn	UTSW	8	33,319,141 (GRCm38)	splice site	probably benign
R1523:Wrn	UTSW	8	33,292,716 (GRCm38)	missense	probably benign	0.23
R1625:Wrn	UTSW	8	33,329,130 (GRCm38)	missense	probably benign	0.01
R1664:Wrn	UTSW	8	33,280,766 (GRCm38)	splice site	probably null
R1773:Wrn	UTSW	8	33,343,561 (GRCm38)	missense	probably damaging	1.00
R1864:Wrn	UTSW	8	33,288,864 (GRCm38)	missense	probably damaging	0.99
R1868:Wrn	UTSW	8	33,257,221 (GRCm38)	missense	probably benign	0.03
R2011:Wrn	UTSW	8	33,236,404 (GRCm38)	missense	probably benign	0.02
R2075:Wrn	UTSW	8	33,322,329 (GRCm38)	missense	probably benign	0.00
R2091:Wrn	UTSW	8	33,267,825 (GRCm38)	missense	probably benign
R2213:Wrn	UTSW	8	33,257,015 (GRCm38)	missense	probably benign	0.05
R2255:Wrn	UTSW	8	33,329,202 (GRCm38)	missense	probably benign	0.13
R2276:Wrn	UTSW	8	33,324,556 (GRCm38)	missense	probably benign	0.02
R3177:Wrn	UTSW	8	33,317,554 (GRCm38)	missense	probably damaging	1.00
R3277:Wrn	UTSW	8	33,317,554 (GRCm38)	missense	probably damaging	1.00
R3779:Wrn	UTSW	8	33,241,020 (GRCm38)	missense	probably damaging	1.00
R3827:Wrn	UTSW	8	33,324,520 (GRCm38)	missense	probably benign	0.00
R4111:Wrn	UTSW	8	33,352,155 (GRCm38)	missense	probably benign	0.02
R4392:Wrn	UTSW	8	33,251,832 (GRCm38)	missense	probably damaging	0.99
R4458:Wrn	UTSW	8	33,294,998 (GRCm38)	missense	probably damaging	0.99
R4650:Wrn	UTSW	8	33,255,509 (GRCm38)	missense	probably benign	0.05
R4656:Wrn	UTSW	8	33,335,991 (GRCm38)	splice site	probably null
R4657:Wrn	UTSW	8	33,335,991 (GRCm38)	splice site	probably null
R4667:Wrn	UTSW	8	33,324,338 (GRCm38)	missense	probably benign	0.00
R4735:Wrn	UTSW	8	33,285,222 (GRCm38)	missense	probably damaging	1.00
R4933:Wrn	UTSW	8	33,322,343 (GRCm38)	missense	probably benign	0.01
R5104:Wrn	UTSW	8	33,267,867 (GRCm38)	splice site	probably null
R5166:Wrn	UTSW	8	33,352,072 (GRCm38)	critical splice donor site	probably null
R5279:Wrn	UTSW	8	33,241,101 (GRCm38)	missense	probably damaging	1.00
R5400:Wrn	UTSW	8	33,294,917 (GRCm38)	missense	probably benign	0.02
R5575:Wrn	UTSW	8	33,336,130 (GRCm38)	missense	probably benign	0.02
R5695:Wrn	UTSW	8	33,324,318 (GRCm38)	missense	probably benign	0.26
R5729:Wrn	UTSW	8	33,268,778 (GRCm38)	missense	probably benign	0.02
R6044:Wrn	UTSW	8	33,236,429 (GRCm38)	missense	probably damaging	1.00
R6139:Wrn	UTSW	8	33,353,332 (GRCm38)	missense	probably damaging	1.00
R6158:Wrn	UTSW	8	33,319,172 (GRCm38)	missense	probably damaging	1.00
R6192:Wrn	UTSW	8	33,284,654 (GRCm38)	missense	probably benign	0.12
R6243:Wrn	UTSW	8	33,284,654 (GRCm38)	missense	possibly damaging	0.94
R6354:Wrn	UTSW	8	33,343,638 (GRCm38)	missense	possibly damaging	0.93
R6429:Wrn	UTSW	8	33,342,996 (GRCm38)	missense	probably damaging	1.00
R6490:Wrn	UTSW	8	33,319,220 (GRCm38)	missense	probably benign	0.01
R6529:Wrn	UTSW	8	33,335,976 (GRCm38)	splice site	probably null
R6535:Wrn	UTSW	8	33,336,103 (GRCm38)	missense	probably damaging	0.99
R7001:Wrn	UTSW	8	33,352,129 (GRCm38)	missense	probably benign	0.04
R7114:Wrn	UTSW	8	33,285,121 (GRCm38)	frame shift	probably null
R7198:Wrn	UTSW	8	33,324,318 (GRCm38)	missense	probably benign	0.00
R7200:Wrn	UTSW	8	33,322,348 (GRCm38)	missense	probably benign	0.00
R7227:Wrn	UTSW	8	33,248,946 (GRCm38)	missense	probably damaging	1.00
R7299:Wrn	UTSW	8	33,292,718 (GRCm38)	missense	probably damaging	1.00
R7374:Wrn	UTSW	8	33,268,911 (GRCm38)	missense	probably damaging	1.00
R7402:Wrn	UTSW	8	33,248,966 (GRCm38)	missense	probably benign	0.00
R7404:Wrn	UTSW	8	33,248,966 (GRCm38)	missense	probably benign	0.00
R7405:Wrn	UTSW	8	33,248,966 (GRCm38)	missense	probably benign	0.00
R7464:Wrn	UTSW	8	33,335,996 (GRCm38)	critical splice donor site	probably null
R7474:Wrn	UTSW	8	33,329,181 (GRCm38)	missense	probably damaging	0.96
R7609:Wrn	UTSW	8	33,310,713 (GRCm38)	missense	possibly damaging	0.50
R7729:Wrn	UTSW	8	33,324,426 (GRCm38)	missense	probably benign	0.21
R7830:Wrn	UTSW	8	33,269,054 (GRCm38)	missense	probably damaging	0.97
R7998:Wrn	UTSW	8	33,292,643 (GRCm38)	missense	probably benign	0.10
R8239:Wrn	UTSW	8	33,329,185 (GRCm38)	missense	probably damaging	1.00
R8262:Wrn	UTSW	8	33,324,246 (GRCm38)	missense	probably benign	0.07
R8410:Wrn	UTSW	8	33,269,020 (GRCm38)	missense	probably damaging	1.00
R8480:Wrn	UTSW	8	33,288,768 (GRCm38)	missense	probably benign	0.10
R8530:Wrn	UTSW	8	33,280,824 (GRCm38)	missense	possibly damaging	0.83
R8540:Wrn	UTSW	8	33,352,126 (GRCm38)	missense	probably damaging	0.96
R8708:Wrn	UTSW	8	33,292,643 (GRCm38)	missense	probably damaging	0.96
R8783:Wrn	UTSW	8	33,336,013 (GRCm38)	missense	probably null	1.00
R8870:Wrn	UTSW	8	33,329,192 (GRCm38)	missense	probably benign	0.01
R8876:Wrn	UTSW	8	33,324,394 (GRCm38)	missense	probably benign	0.00
R9050:Wrn	UTSW	8	33,342,993 (GRCm38)	missense	probably damaging	1.00
R9329:Wrn	UTSW	8	33,240,978 (GRCm38)	missense	probably benign
R9595:Wrn	UTSW	8	33,268,933 (GRCm38)	missense	probably benign
R9621:Wrn	UTSW	8	33,324,273 (GRCm38)	missense	probably benign	0.01
R9623:Wrn	UTSW	8	33,284,616 (GRCm38)	critical splice donor site	probably null
R9797:Wrn	UTSW	8	33,268,922 (GRCm38)	missense	probably benign	0.02
RF010:Wrn	UTSW	8	33,288,765 (GRCm38)	missense	probably benign	0.13
X0017:Wrn	UTSW	8	33,280,782 (GRCm38)	missense	probably damaging	1.00
Z1176:Wrn	UTSW	8	33,334,209 (GRCm38)	missense	probably damaging	0.98

Predicted Primers

PCR Primer
(F):5'- AGCCTGTTAAGCTCTGTATGTCTTGC -3'
(R):5'- GGTGGAAGACCCTTTCTCACCATCTC -3'

Sequencing Primer
(F):5'- GCAGTCGGATACTTGATATGAACC -3'
(R):5'- TCTCACCATCTCATAGCTGAAGG -3'

Posted On

2014-03-17