Mutagenetix > Incidental Mutations

Incidental Mutation 'R1393:Zfp810'

162699

Institutional Source

Beutler Lab

Gene Symbol

Zfp810

Ensembl Gene

ENSMUSG00000066829

Gene Name

zinc finger protein 810

Synonyms

MMRRC Submission

039455-MU

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.092) question?

Stock #

R1393 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

22276748-22307648 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 22280514 bp

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glycine at position 90 (D90G)

Ref Sequence

ENSEMBL: ENSMUSP00000083459 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000086278] [ENSMUST00000215202]

Predicted Effect

probably benign
Transcript: ENSMUST00000086278
AA Change: D90G

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000083459
Gene: ENSMUSG00000066829
AA Change: D90G

Domain	Start	End	E-Value	Type
KRAB	4	64	1.09e-33	SMART
ZnF_C2H2	126	148	2.44e2	SMART
ZnF_C2H2	182	204	3.07e-1	SMART
ZnF_C2H2	210	232	8.47e-4	SMART
ZnF_C2H2	238	260	6.78e-3	SMART
ZnF_C2H2	266	288	6.13e-1	SMART
ZnF_C2H2	294	316	5.06e-2	SMART
ZnF_C2H2	322	344	4.79e-3	SMART
ZnF_C2H2	350	372	2.99e-4	SMART
ZnF_C2H2	378	400	1.33e-1	SMART
ZnF_C2H2	406	428	2.75e-3	SMART
ZnF_C2H2	434	456	1.58e-3	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000214499

Predicted Effect

probably benign
Transcript: ENSMUST00000215202

Coding Region Coverage

1x: 98.9%
3x: 97.9%
10x: 95.1%
20x: 88.7%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 25 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcf3	A	G	16: 20,560,430	N682S	probably benign	Het
Acta2	G	A	19: 34,241,792	R337C	probably damaging	Het
Anxa5	G	A	3: 36,453,509	T194I	probably damaging	Het
Atf1	A	G	15: 100,232,766	T6A	possibly damaging	Het
Atg4d	T	A	9: 21,270,833	Y317N	probably damaging	Het
Bcl6	A	G	16: 23,977,566	V37A	probably damaging	Het
Bsn	T	C	9: 108,110,517		probably benign	Het
Cd300ld2	G	A	11: 115,012,578		probably benign	Het
Cdh15	G	C	8: 122,857,495	E112Q	probably damaging	Het
Chad	A	T	11: 94,565,314	M73L	probably benign	Het
Copz1	A	G	15: 103,294,744	N95S	probably benign	Het
Cwf19l2	T	C	9: 3,456,818	V717A	probably benign	Het
Dock3	C	T	9: 106,911,349	G140R	probably damaging	Het
Gm14226	A	T	2: 155,024,191	S23C	probably damaging	Het
Gria2	T	C	3: 80,707,098	E545G	probably damaging	Het
Nxpe2	T	C	9: 48,326,614	T114A	probably damaging	Het
Olfr811	A	G	10: 129,801,932	F198L	probably benign	Het
Patj	G	A	4: 98,424,411	V329I	probably benign	Het
Ptcd3	T	A	6: 71,889,621	T404S	probably benign	Het
Rasa1	A	G	13: 85,223,522	C867R	probably damaging	Het
Rps24	A	G	14: 24,491,762	T6A	probably damaging	Het
Rsad2	T	C	12: 26,456,377	S15G	probably damaging	Het
Serpina12	T	C	12: 104,037,750	I208V	possibly damaging	Het
Spock1	A	T	13: 57,907,454	L45Q	probably damaging	Het
Stat3	A	T	11: 100,888,765		probably null	Het

Other mutations in Zfp810

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00484:Zfp810	APN	9	22278309	nonsense	probably null
IGL03079:Zfp810	APN	9	22284127	missense	probably damaging	1.00
IGL03402:Zfp810	APN	9	22279145	utr 3 prime	probably null
H8562:Zfp810	UTSW	9	22279091	missense	probably benign	0.42
R1116:Zfp810	UTSW	9	22279085	missense	probably benign	0.11
R1160:Zfp810	UTSW	9	22278532	missense	possibly damaging	0.64
R1171:Zfp810	UTSW	9	22278826	missense	possibly damaging	0.95
R1608:Zfp810	UTSW	9	22278920	missense	probably benign	0.00
R1644:Zfp810	UTSW	9	22279028	missense	possibly damaging	0.67
R1766:Zfp810	UTSW	9	22278532	missense	possibly damaging	0.64
R2568:Zfp810	UTSW	9	22279238	missense	probably benign	0.01
R3684:Zfp810	UTSW	9	22278235	missense	probably benign	0.01
R4002:Zfp810	UTSW	9	22278892	missense	probably damaging	1.00
R4134:Zfp810	UTSW	9	22279073	missense	probably damaging	0.97
R4135:Zfp810	UTSW	9	22279073	missense	probably damaging	0.97
R4334:Zfp810	UTSW	9	22278784	missense	probably benign	0.00
R4545:Zfp810	UTSW	9	22278745	missense	probably damaging	0.96
R5399:Zfp810	UTSW	9	22278829	missense	possibly damaging	0.91
R5622:Zfp810	UTSW	9	22279096	missense	probably benign	0.00
R5643:Zfp810	UTSW	9	22283171	missense	probably benign	0.26
R7375:Zfp810	UTSW	9	22290537	critical splice donor site	probably null
R7441:Zfp810	UTSW	9	22279272	nonsense	probably null
R7809:Zfp810	UTSW	9	22278982	missense	possibly damaging	0.51

Predicted Primers

PCR Primer
(F):5'- CAGAGTTATTTCCCCAGATGCCTCG -3'
(R):5'- AGCTGTCAGTGTGCAGTGTAATTCC -3'

Sequencing Primer
(F):5'- GACTTGACTGCTCAGTCTGATATTC -3'
(R):5'- CCAATTCATTGAAGACTAGGATGTGC -3'

Posted On

2014-03-17