Mutagenetix > Incidental Mutations

Incidental Mutation 'R1489:Vmn1r174'

163597

Institutional Source

Beutler Lab

Gene Symbol

Vmn1r174

Ensembl Gene

ENSMUSG00000090411

Gene Name

vomeronasal 1 receptor 174

Synonyms

V1rd22

MMRRC Submission

039541-MU

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.060) question?

Stock #

R1489 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

23752270-23762403 bp(+) (GRCm38)

Type of Mutation

nonsense

DNA Base Change (assembly)

C to T at 23754556 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Glutamine to Stop codon at position 216 (Q216*)

Ref Sequence

ENSEMBL: ENSMUSP00000154506 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000167551] [ENSMUST00000228331]

AlphaFold

E9PYW5

Predicted Effect

probably null
Transcript: ENSMUST00000167551
AA Change: Q216*

SMART Domains

Protein: ENSMUSP00000126478
Gene: ENSMUSG00000090411
AA Change: Q216*

Domain	Start	End	E-Value	Type
Pfam:TAS2R	8	300	4e-9	PFAM
Pfam:V1R	43	300	5e-19	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000228331
AA Change: Q216*

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 99.2%
3x: 98.4%
10x: 96.7%
20x: 93.9%

Validation Efficiency

96% (52/54)

Allele List at MGI

Other mutations in this stock

Total: 49 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2700097O09Rik	A	G	12: 55,059,510	S143P	possibly damaging	Het
4930522H14Rik	T	C	4: 109,505,457	K218E	possibly damaging	Het
Abcb1a	T	A	5: 8,686,300		probably null	Het
Adam39	A	G	8: 40,824,994	T141A	possibly damaging	Het
Adam6b	T	A	12: 113,491,451	S629R	probably benign	Het
Ap3b2	C	A	7: 81,463,690	E924*	probably null	Het
Armc3	A	T	2: 19,310,047	Y856F	probably benign	Het
Asap1	A	G	15: 64,172,730	L142P	probably damaging	Het
Atg9a	T	A	1: 75,186,090	D427V	probably damaging	Het
Atl2	G	A	17: 79,852,706	A17V	probably benign	Het
Atxn2l	A	T	7: 126,496,467	S379T	probably damaging	Het
C1ql3	G	T	2: 13,010,642	P69Q	possibly damaging	Het
Cap2	A	T	13: 46,609,635	I114F	probably damaging	Het
Cox16	T	C	12: 81,474,615	N135S	probably null	Het
Dnajc13	A	T	9: 104,231,035	H180Q	possibly damaging	Het
Dpy19l3	A	T	7: 35,725,410	Y73*	probably null	Het
Duox2	T	A	2: 122,293,396	M436L	probably benign	Het
Exoc6	T	A	19: 37,597,120	M481K	possibly damaging	Het
Fbxw2	GCCCCC	GCCCCCCCC	2: 34,812,817		probably benign	Het
Fmn1	T	A	2: 113,365,212	V419E	unknown	Het
Fndc4	T	C	5: 31,293,451	*232W	probably null	Het
Foxc1	C	T	13: 31,808,612	R469*	probably null	Het
Fsip2	A	G	2: 82,979,811	H2158R	probably benign	Het
Ghdc	C	T	11: 100,768,257	G373D	probably benign	Het
Gm10330	A	T	12: 23,780,031	S50T	probably benign	Het
Gm8251	A	G	1: 44,057,790	F1383L	probably benign	Het
Gm8251	T	C	1: 44,061,507	I144V	probably benign	Het
Hlx	G	T	1: 184,731,987	A52D	probably damaging	Het
Lonrf1	A	G	8: 36,222,954	V650A	probably damaging	Het
Map1a	T	C	2: 121,300,437	V578A	possibly damaging	Het
Mbd3	C	G	10: 80,393,906	D190H	probably damaging	Het
Mcpt9	T	C	14: 56,027,519	K175R	probably benign	Het
Mia3	A	G	1: 183,338,674	S85P	probably benign	Het
Myrip	T	C	9: 120,432,529	F403L	probably damaging	Het
Nox4	A	G	7: 87,304,889	Y134C	probably damaging	Het
Numb	A	G	12: 83,795,443	L642P	probably damaging	Het
Pappa	A	T	4: 65,180,948	Y568F	possibly damaging	Het
Pdzrn4	T	C	15: 92,677,712	L333P	probably benign	Het
Prl	A	T	13: 27,057,636	S3C	probably damaging	Het
Ptprc	T	C	1: 138,120,086	T60A	possibly damaging	Het
Rbm10	C	T	X: 20,637,664		probably benign	Het
Slc7a7	C	T	14: 54,408,646	R120H	probably damaging	Het
Smpd1	T	C	7: 105,556,554		probably null	Het
Spta1	A	G	1: 174,231,325	E1942G	probably damaging	Het
Tmem38a	A	G	8: 72,579,635	Y66C	probably damaging	Het
Tnnt1	A	T	7: 4,507,525	Y232*	probably null	Het
Tpte	T	C	8: 22,349,389		probably null	Het
Virma	T	C	4: 11,521,164	V907A	probably damaging	Het
Zswim3	T	C	2: 164,819,981	V127A	probably benign	Het

Other mutations in Vmn1r174

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00331:Vmn1r174	APN	7	23754533	missense	possibly damaging	0.77
IGL00950:Vmn1r174	APN	7	23754486	missense	possibly damaging	0.96
IGL01484:Vmn1r174	APN	7	23754324	nonsense	probably null
IGL02014:Vmn1r174	APN	7	23754158	missense	probably damaging	1.00
IGL02190:Vmn1r174	APN	7	23754827	missense	unknown
IGL03265:Vmn1r174	APN	7	23754473	nonsense	probably null
IGL03335:Vmn1r174	APN	7	23754512	missense	probably benign	0.41
R0529:Vmn1r174	UTSW	7	23754197	missense	probably benign	0.00
R1645:Vmn1r174	UTSW	7	23754352	missense	possibly damaging	0.87
R1691:Vmn1r174	UTSW	7	23753912	start codon destroyed	probably null	1.00
R1753:Vmn1r174	UTSW	7	23754197	missense	probably benign	0.00
R1939:Vmn1r174	UTSW	7	23754107	missense	probably damaging	0.99
R1988:Vmn1r174	UTSW	7	23754625	missense	probably damaging	0.98
R2299:Vmn1r174	UTSW	7	23754004	missense	probably benign	0.08
R4429:Vmn1r174	UTSW	7	23754140	missense	probably benign	0.02
R4516:Vmn1r174	UTSW	7	23754343	missense	probably benign	0.01
R4589:Vmn1r174	UTSW	7	23754779	nonsense	probably null
R5175:Vmn1r174	UTSW	7	23754728	missense	probably benign	0.03
R5392:Vmn1r174	UTSW	7	23754802	missense	unknown
R5503:Vmn1r174	UTSW	7	23754137	missense	probably benign	0.03
R5568:Vmn1r174	UTSW	7	23754494	missense	probably damaging	0.96
R6705:Vmn1r174	UTSW	7	23754426	missense	probably benign	0.25
R8168:Vmn1r174	UTSW	7	23754671	missense	probably damaging	0.99
R8190:Vmn1r174	UTSW	7	23754143	missense	probably damaging	1.00
R8523:Vmn1r174	UTSW	7	23754757	missense	probably damaging	1.00
R8555:Vmn1r174	UTSW	7	23754545	missense	possibly damaging	0.84
R8913:Vmn1r174	UTSW	7	23753950	missense	possibly damaging	0.90
R8979:Vmn1r174	UTSW	7	23754467	missense	possibly damaging	0.92
R8990:Vmn1r174	UTSW	7	23754531	missense	possibly damaging	0.71
X0011:Vmn1r174	UTSW	7	23754481	missense	probably benign	0.03

Predicted Primers

PCR Primer
(F):5'- TTTGCTGGCCCTCAACTGAGAC -3'
(R):5'- TGCAGCCAAAATTTCACTGACATGC -3'

Sequencing Primer
(F):5'- CTCACTGGGTTTCCAAGCAATATG -3'
(R):5'- ATTTCACTGACATGCCTCAAC -3'

Posted On

2014-03-28