Mutagenetix > Incidental Mutation

Incidental Mutation 'R1476:Unc79'

164088

Institutional Source

Beutler Lab

Gene Symbol

Unc79

Ensembl Gene

ENSMUSG00000021198

Gene Name

unc-79 homolog

Synonyms

9030205A07Rik, Mlca3

MMRRC Submission

039529-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R1476 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

102915118-103150324 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 103149784 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Leucine to Proline at position 2626 (L2626P)

Ref Sequence

ENSEMBL: ENSMUSP00000136332 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000085079] [ENSMUST00000101099] [ENSMUST00000178076] [ENSMUST00000179002]

AlphaFold

Q0KK59

Predicted Effect

possibly damaging
Transcript: ENSMUST00000085079
AA Change: L2430P

PolyPhen 2 Score 0.956 (Sensitivity: 0.79; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000082156
Gene: ENSMUSG00000021198
AA Change: L2430P

Domain	Start	End	E-Value	Type
Pfam:UNC-79	1	469	3.1e-223	PFAM
low complexity region	732	737	N/A	INTRINSIC
low complexity region	846	862	N/A	INTRINSIC
low complexity region	968	977	N/A	INTRINSIC
low complexity region	1114	1125	N/A	INTRINSIC
low complexity region	1313	1325	N/A	INTRINSIC
low complexity region	1428	1440	N/A	INTRINSIC
low complexity region	1471	1476	N/A	INTRINSIC
low complexity region	1477	1489	N/A	INTRINSIC
low complexity region	1490	1504	N/A	INTRINSIC
low complexity region	1541	1556	N/A	INTRINSIC
low complexity region	1861	1870	N/A	INTRINSIC
low complexity region	2237	2246	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000101099
AA Change: L2568P

PolyPhen 2 Score 0.996 (Sensitivity: 0.55; Specificity: 0.98)

SMART Domains

Protein: ENSMUSP00000098659
Gene: ENSMUSG00000021198
AA Change: L2568P

Domain	Start	End	E-Value	Type
Pfam:UNC-79	113	646	1.2e-226	PFAM
low complexity region	909	914	N/A	INTRINSIC
low complexity region	1023	1039	N/A	INTRINSIC
low complexity region	1145	1154	N/A	INTRINSIC
low complexity region	1291	1302	N/A	INTRINSIC
low complexity region	1490	1502	N/A	INTRINSIC
low complexity region	1605	1617	N/A	INTRINSIC
low complexity region	1648	1653	N/A	INTRINSIC
low complexity region	1654	1666	N/A	INTRINSIC
low complexity region	1667	1681	N/A	INTRINSIC
low complexity region	1718	1733	N/A	INTRINSIC
low complexity region	1999	2008	N/A	INTRINSIC
low complexity region	2375	2384	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000178076
AA Change: L2433P

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000136888
Gene: ENSMUSG00000021198
AA Change: L2433P

Domain	Start	End	E-Value	Type
Pfam:UNC-79	1	450	4.2e-213	PFAM
low complexity region	713	718	N/A	INTRINSIC
low complexity region	827	843	N/A	INTRINSIC
low complexity region	949	958	N/A	INTRINSIC
low complexity region	1117	1128	N/A	INTRINSIC
low complexity region	1316	1328	N/A	INTRINSIC
low complexity region	1431	1443	N/A	INTRINSIC
low complexity region	1474	1479	N/A	INTRINSIC
low complexity region	1480	1492	N/A	INTRINSIC
low complexity region	1493	1507	N/A	INTRINSIC
low complexity region	1544	1559	N/A	INTRINSIC
low complexity region	1864	1873	N/A	INTRINSIC
low complexity region	2240	2249	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000179002
AA Change: L2626P

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000136332
Gene: ENSMUSG00000021198
AA Change: L2626P

Domain	Start	End	E-Value	Type
Pfam:UNC-79	60	593	1.3e-226	PFAM
low complexity region	856	861	N/A	INTRINSIC
low complexity region	970	986	N/A	INTRINSIC
low complexity region	1092	1101	N/A	INTRINSIC
low complexity region	1260	1271	N/A	INTRINSIC
low complexity region	1509	1521	N/A	INTRINSIC
low complexity region	1624	1636	N/A	INTRINSIC
low complexity region	1667	1672	N/A	INTRINSIC
low complexity region	1673	1685	N/A	INTRINSIC
low complexity region	1686	1700	N/A	INTRINSIC
low complexity region	1737	1752	N/A	INTRINSIC
low complexity region	2057	2066	N/A	INTRINSIC
low complexity region	2433	2442	N/A	INTRINSIC

Meta Mutation Damage Score

0.9484

Coding Region Coverage

1x: 99.3%
3x: 98.4%
10x: 96.5%
20x: 93.1%

Validation Efficiency

95% (80/84)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The NALCN channel is responsible for Na(+) leak currents. The protein encoded by this gene, along with UNC80, is an accessory subunit of the NALCN channel that contributes to the Ca(2+) sensitivity of the channel. [provided by RefSeq, Sep 2016]
PHENOTYPE: Homozygous mutation results in lethality within the first week after birth, mostly at P0 or P1. Pups fail to nurse and have no milk in stomachs resulting in weakness, inactivity and no weight gain. [provided by MGI curators]

Allele List at MGI

All alleles(2) : Targeted, knock-out(1) Chemically induced(1)

Other mutations in this stock

Total: 78 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2310033P09Rik	A	G	11: 59,099,528 (GRCm39)		probably benign	Het
A730015C16Rik	G	A	4: 108,705,205 (GRCm39)	V40M	probably damaging	Het
Abcg2	A	G	6: 58,655,322 (GRCm39)	D419G	probably benign	Het
Adamts17	A	C	7: 66,725,091 (GRCm39)	E777A	probably damaging	Het
Ak1	A	G	2: 32,523,478 (GRCm39)	K166R	probably benign	Het
Ankrd12	T	C	17: 66,293,300 (GRCm39)	K711R	probably damaging	Het
Ate1	A	T	7: 130,020,301 (GRCm39)		probably null	Het
Atp5f1a	A	G	18: 77,869,625 (GRCm39)	H519R	probably benign	Het
Best1	G	T	19: 9,967,853 (GRCm39)	Y284*	probably null	Het
C2cd6	A	G	1: 59,115,887 (GRCm39)		probably benign	Het
Casz1	G	T	4: 149,030,628 (GRCm39)	V1216L	probably benign	Het
Cdc42bpg	A	G	19: 6,363,812 (GRCm39)	D493G	probably damaging	Het
Ces2f	A	G	8: 105,679,134 (GRCm39)	D317G	possibly damaging	Het
Chst15	A	T	7: 131,872,002 (GRCm39)	M93K	possibly damaging	Het
Cntnap5a	T	C	1: 115,828,750 (GRCm39)	L58P	probably damaging	Het
Crip3	T	C	17: 46,741,702 (GRCm39)		probably benign	Het
Csmd2	G	A	4: 128,380,794 (GRCm39)	E2117K	probably benign	Het
Cstf3	A	G	2: 104,478,564 (GRCm39)	D212G	possibly damaging	Het
Cttnbp2	T	C	6: 18,434,220 (GRCm39)	K546R	probably damaging	Het
Cubn	A	G	2: 13,480,931 (GRCm39)	I308T	probably benign	Het
Cxcl9	T	A	5: 92,472,972 (GRCm39)	D75V	probably damaging	Het
Dcdc5	C	T	2: 106,188,977 (GRCm39)		noncoding transcript	Het
Defa30	C	A	8: 21,624,752 (GRCm39)	T25K	possibly damaging	Het
Dock7	A	T	4: 98,967,672 (GRCm39)	H239Q	possibly damaging	Het
Dpp4	A	G	2: 62,178,245 (GRCm39)	V629A	possibly damaging	Het
Fam83a	T	C	15: 57,873,341 (GRCm39)	M390T	probably benign	Het
Fem1c	A	G	18: 46,657,552 (GRCm39)	L54P	probably damaging	Het
Fntb	T	A	12: 76,957,007 (GRCm39)	M282K	probably benign	Het
Galnt2l	A	G	8: 122,996,323 (GRCm39)		probably benign	Het
Gm11099	G	A	2: 58,749,482 (GRCm39)		probably benign	Het
Gm1527	T	C	3: 28,980,705 (GRCm39)	S602P	probably benign	Het
Gm5478	T	A	15: 101,553,080 (GRCm39)	I331F	probably damaging	Het
Gm7853	C	A	14: 35,811,540 (GRCm39)		noncoding transcript	Het
Gsta2	A	T	9: 78,249,147 (GRCm39)	C18S	probably benign	Het
H1f8	T	C	6: 115,924,701 (GRCm39)	V69A	possibly damaging	Het
Hecw1	T	C	13: 14,480,671 (GRCm39)	E465G	probably damaging	Het
Herc1	A	G	9: 66,415,548 (GRCm39)	D4841G	probably damaging	Het
Hus1b	A	G	13: 31,130,984 (GRCm39)	V225A	probably benign	Het
Keg1	A	T	19: 12,693,387 (GRCm39)	M137L	probably benign	Het
Kmt2a	C	T	9: 44,735,932 (GRCm39)		probably benign	Het
Megf6	G	T	4: 154,261,578 (GRCm39)	V68L	probably benign	Het
Mga	T	A	2: 119,772,156 (GRCm39)	V1672E	probably damaging	Het
Mios	T	C	6: 8,234,237 (GRCm39)	S803P	probably benign	Het
Mrtfa	T	C	15: 80,902,409 (GRCm39)		probably benign	Het
Msh4	A	T	3: 153,569,021 (GRCm39)	Y851N	probably damaging	Het
Mybl1	A	C	1: 9,742,886 (GRCm39)		probably null	Het
Myo5c	A	G	9: 75,183,221 (GRCm39)	Y865C	probably damaging	Het
Naip1	T	C	13: 100,563,378 (GRCm39)	S596G	probably benign	Het
Nek5	G	T	8: 22,586,747 (GRCm39)	Q355K	possibly damaging	Het
Nphp3	G	T	9: 103,903,126 (GRCm39)	R701L	possibly damaging	Het
Or52n2	A	T	7: 104,542,444 (GRCm39)	Y130*	probably null	Het
Or5j3	T	C	2: 86,128,823 (GRCm39)	I221T	probably damaging	Het
Or5m10	T	A	2: 85,717,592 (GRCm39)	Y149*	probably null	Het
Or5t9	T	C	2: 86,659,542 (GRCm39)	S149P	probably benign	Het
Or9g4	T	A	2: 85,505,262 (GRCm39)	T78S	possibly damaging	Het
Palm	A	G	10: 79,651,021 (GRCm39)	N149D	possibly damaging	Het
Pot1b	T	C	17: 55,960,451 (GRCm39)	I626M	possibly damaging	Het
Ptprt	T	A	2: 161,769,404 (GRCm39)	D487V	probably damaging	Het
Qpct	A	G	17: 79,378,201 (GRCm39)	I124V	probably benign	Het
Rbm12b1	T	A	4: 12,145,817 (GRCm39)	D596E	possibly damaging	Het
Rnf157	A	G	11: 116,245,585 (GRCm39)	C277R	probably damaging	Het
Rnf169	A	G	7: 99,574,535 (GRCm39)	S687P	possibly damaging	Het
Sfxn1	T	A	13: 54,246,469 (GRCm39)		probably null	Het
Slc6a21	T	C	7: 44,922,052 (GRCm39)	V649A	probably benign	Het
Slit3	A	T	11: 35,577,126 (GRCm39)	T1120S	probably damaging	Het
Spem2	A	T	11: 69,708,896 (GRCm39)	M58K	probably benign	Het
Sprr2k	T	A	3: 92,340,703 (GRCm39)		probably benign	Het
Sspo	T	C	6: 48,440,334 (GRCm39)		probably null	Het
Sv2b	A	T	7: 74,769,791 (GRCm39)	F584I	possibly damaging	Het
Tkfc	A	T	19: 10,572,690 (GRCm39)	M317K	probably null	Het
Tnni3k	C	T	3: 154,735,942 (GRCm39)	G134S	probably benign	Het
Ttn	C	T	2: 76,570,124 (GRCm39)	R26923H	probably damaging	Het
Tuba3b	T	C	6: 145,564,179 (GRCm39)	V75A	possibly damaging	Het
Usp24	A	T	4: 106,219,130 (GRCm39)	I491F	probably damaging	Het
V1ra8	A	T	6: 90,180,132 (GRCm39)	I112F	probably damaging	Het
Vmn1r29	T	G	6: 58,284,663 (GRCm39)	F128V	probably benign	Het
Wdr87-ps	A	G	7: 29,234,315 (GRCm39)		noncoding transcript	Het
Zfp157	T	A	5: 138,453,357 (GRCm39)		probably null	Het

Other mutations in Unc79

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00718:Unc79	APN	12	103,135,906 (GRCm39)	missense	possibly damaging	0.68
IGL00835:Unc79	APN	12	103,108,149 (GRCm39)	splice site	probably benign
IGL00917:Unc79	APN	12	103,054,766 (GRCm39)	missense	possibly damaging	0.53
IGL01012:Unc79	APN	12	103,078,714 (GRCm39)	missense	probably damaging	1.00
IGL01121:Unc79	APN	12	103,131,890 (GRCm39)	missense	probably damaging	0.99
IGL01303:Unc79	APN	12	103,128,126 (GRCm39)	missense	possibly damaging	0.94
IGL01305:Unc79	APN	12	102,968,130 (GRCm39)	missense	probably damaging	0.99
IGL01315:Unc79	APN	12	103,054,780 (GRCm39)	missense	possibly damaging	0.66
IGL01388:Unc79	APN	12	103,136,018 (GRCm39)	splice site	probably benign
IGL01415:Unc79	APN	12	103,074,944 (GRCm39)	missense	probably damaging	1.00
IGL01447:Unc79	APN	12	103,045,177 (GRCm39)	missense	probably damaging	1.00
IGL01655:Unc79	APN	12	103,134,546 (GRCm39)	missense	probably benign	0.00
IGL01662:Unc79	APN	12	103,115,279 (GRCm39)	missense	possibly damaging	0.92
IGL01728:Unc79	APN	12	103,131,943 (GRCm39)	missense	probably damaging	0.98
IGL01767:Unc79	APN	12	103,108,256 (GRCm39)	missense	probably damaging	1.00
IGL02080:Unc79	APN	12	102,968,234 (GRCm39)	missense	probably damaging	1.00
IGL02115:Unc79	APN	12	102,964,933 (GRCm39)	missense	probably damaging	1.00
IGL02176:Unc79	APN	12	102,965,006 (GRCm39)	splice site	probably null
IGL02186:Unc79	APN	12	102,977,542 (GRCm39)	missense	probably benign	0.04
IGL02205:Unc79	APN	12	103,045,260 (GRCm39)	missense	probably damaging	1.00
IGL02337:Unc79	APN	12	103,122,705 (GRCm39)	splice site	probably benign
IGL02498:Unc79	APN	12	103,137,837 (GRCm39)	missense	probably damaging	0.99
IGL02508:Unc79	APN	12	103,078,535 (GRCm39)	missense	probably damaging	0.97
IGL02508:Unc79	APN	12	103,078,277 (GRCm39)	splice site	probably benign
IGL02557:Unc79	APN	12	103,148,418 (GRCm39)	splice site	probably benign
IGL02589:Unc79	APN	12	103,139,755 (GRCm39)	missense	probably damaging	1.00
IGL02611:Unc79	APN	12	103,131,967 (GRCm39)	missense	probably damaging	0.97
IGL02728:Unc79	APN	12	103,088,688 (GRCm39)	missense	possibly damaging	0.53
IGL02827:Unc79	APN	12	103,041,105 (GRCm39)	missense	possibly damaging	0.88
IGL03028:Unc79	APN	12	103,139,785 (GRCm39)	missense	possibly damaging	0.83
IGL03144:Unc79	APN	12	103,008,401 (GRCm39)	missense	probably damaging	1.00
IGL03229:Unc79	APN	12	103,100,798 (GRCm39)	missense	probably damaging	0.99
IGL03269:Unc79	APN	12	103,054,936 (GRCm39)	missense	probably damaging	1.00
IGL03325:Unc79	APN	12	103,135,869 (GRCm39)	missense	probably damaging	0.98
pencil-thin	UTSW	12	103,075,040 (GRCm39)	splice site	probably null
sweetpea	UTSW	12	103,025,777 (GRCm39)	missense	probably damaging	1.00
3-1:Unc79	UTSW	12	103,039,009 (GRCm39)	nonsense	probably null
ANU22:Unc79	UTSW	12	102,968,130 (GRCm39)	missense	probably damaging	0.99
R0046:Unc79	UTSW	12	103,091,940 (GRCm39)	missense	probably damaging	0.99
R0046:Unc79	UTSW	12	103,091,940 (GRCm39)	missense	probably damaging	0.99
R0067:Unc79	UTSW	12	103,025,777 (GRCm39)	missense	probably damaging	1.00
R0067:Unc79	UTSW	12	103,025,777 (GRCm39)	missense	probably damaging	1.00
R0107:Unc79	UTSW	12	103,100,784 (GRCm39)	missense	possibly damaging	0.70
R0110:Unc79	UTSW	12	103,045,329 (GRCm39)	critical splice donor site	probably null
R0128:Unc79	UTSW	12	103,054,693 (GRCm39)	splice site	probably benign
R0166:Unc79	UTSW	12	103,122,812 (GRCm39)	missense	probably damaging	1.00
R0208:Unc79	UTSW	12	103,058,286 (GRCm39)	missense	probably benign	0.00
R0211:Unc79	UTSW	12	103,039,051 (GRCm39)	missense	probably benign	0.01
R0211:Unc79	UTSW	12	103,039,051 (GRCm39)	missense	probably benign	0.01
R0218:Unc79	UTSW	12	103,075,040 (GRCm39)	splice site	probably null
R0244:Unc79	UTSW	12	103,079,150 (GRCm39)	missense	probably damaging	1.00
R0305:Unc79	UTSW	12	103,079,459 (GRCm39)	missense	probably benign	0.18
R0310:Unc79	UTSW	12	103,027,666 (GRCm39)	missense	probably damaging	1.00
R0325:Unc79	UTSW	12	103,137,903 (GRCm39)	missense	probably damaging	0.98
R0369:Unc79	UTSW	12	103,055,031 (GRCm39)	critical splice donor site	probably null
R0450:Unc79	UTSW	12	103,045,329 (GRCm39)	critical splice donor site	probably null
R0503:Unc79	UTSW	12	103,045,127 (GRCm39)	missense	probably benign	0.01
R0542:Unc79	UTSW	12	103,060,437 (GRCm39)	splice site	probably benign
R0845:Unc79	UTSW	12	103,139,703 (GRCm39)	splice site	probably benign
R0893:Unc79	UTSW	12	102,957,687 (GRCm39)	missense	probably damaging	1.00
R1078:Unc79	UTSW	12	103,041,112 (GRCm39)	missense	probably benign	0.03
R1148:Unc79	UTSW	12	103,078,926 (GRCm39)	missense	probably damaging	1.00
R1148:Unc79	UTSW	12	103,078,926 (GRCm39)	missense	probably damaging	1.00
R1159:Unc79	UTSW	12	103,013,311 (GRCm39)	splice site	probably benign
R1191:Unc79	UTSW	12	103,013,271 (GRCm39)	nonsense	probably null
R1307:Unc79	UTSW	12	103,036,335 (GRCm39)	missense	probably damaging	1.00
R1368:Unc79	UTSW	12	103,122,772 (GRCm39)	missense	probably damaging	1.00
R1650:Unc79	UTSW	12	103,079,052 (GRCm39)	missense	possibly damaging	0.85
R1777:Unc79	UTSW	12	103,078,714 (GRCm39)	missense	probably damaging	1.00
R1796:Unc79	UTSW	12	103,109,005 (GRCm39)	missense	probably damaging	0.99
R1824:Unc79	UTSW	12	103,025,579 (GRCm39)	missense	probably damaging	1.00
R1830:Unc79	UTSW	12	103,100,737 (GRCm39)	missense	probably damaging	1.00
R1927:Unc79	UTSW	12	103,135,951 (GRCm39)	missense	probably damaging	1.00
R1958:Unc79	UTSW	12	103,041,178 (GRCm39)	missense	probably benign	0.19
R1958:Unc79	UTSW	12	102,957,621 (GRCm39)	missense	probably damaging	1.00
R1980:Unc79	UTSW	12	102,977,538 (GRCm39)	nonsense	probably null
R2019:Unc79	UTSW	12	103,137,830 (GRCm39)	critical splice acceptor site	probably null
R2290:Unc79	UTSW	12	103,112,625 (GRCm39)	missense	probably damaging	1.00
R2939:Unc79	UTSW	12	102,957,684 (GRCm39)	missense	probably damaging	1.00
R2962:Unc79	UTSW	12	103,061,378 (GRCm39)	missense	possibly damaging	0.72
R3176:Unc79	UTSW	12	103,079,476 (GRCm39)	missense	probably damaging	1.00
R3276:Unc79	UTSW	12	103,079,476 (GRCm39)	missense	probably damaging	1.00
R3683:Unc79	UTSW	12	103,041,062 (GRCm39)	missense	probably benign	0.00
R3684:Unc79	UTSW	12	103,041,062 (GRCm39)	missense	probably benign	0.00
R3686:Unc79	UTSW	12	103,054,920 (GRCm39)	missense	probably damaging	1.00
R3760:Unc79	UTSW	12	103,058,964 (GRCm39)	missense	probably damaging	1.00
R4031:Unc79	UTSW	12	103,039,018 (GRCm39)	missense	possibly damaging	0.46
R4039:Unc79	UTSW	12	103,041,208 (GRCm39)	missense	possibly damaging	0.88
R4110:Unc79	UTSW	12	103,025,629 (GRCm39)	missense	probably damaging	1.00
R4113:Unc79	UTSW	12	103,025,629 (GRCm39)	missense	probably damaging	1.00
R4159:Unc79	UTSW	12	103,036,512 (GRCm39)	intron	probably benign
R4273:Unc79	UTSW	12	103,088,612 (GRCm39)	missense	probably damaging	0.99
R4292:Unc79	UTSW	12	103,149,703 (GRCm39)	missense	probably damaging	0.99
R4334:Unc79	UTSW	12	103,045,233 (GRCm39)	missense	probably benign
R4513:Unc79	UTSW	12	102,988,019 (GRCm39)	missense	probably damaging	1.00
R4562:Unc79	UTSW	12	102,957,720 (GRCm39)	missense	probably damaging	1.00
R4576:Unc79	UTSW	12	102,968,062 (GRCm39)	splice site	probably benign
R4645:Unc79	UTSW	12	103,079,081 (GRCm39)	missense	probably benign
R4758:Unc79	UTSW	12	103,128,080 (GRCm39)	nonsense	probably null
R4787:Unc79	UTSW	12	103,013,257 (GRCm39)	missense	probably damaging	1.00
R4852:Unc79	UTSW	12	103,139,725 (GRCm39)	missense	probably damaging	0.98
R4883:Unc79	UTSW	12	103,060,592 (GRCm39)	missense	probably damaging	0.99
R4898:Unc79	UTSW	12	103,128,079 (GRCm39)	missense	probably damaging	0.99
R4979:Unc79	UTSW	12	103,078,691 (GRCm39)	missense	probably benign
R5044:Unc79	UTSW	12	103,078,962 (GRCm39)	missense	probably benign	0.32
R5053:Unc79	UTSW	12	103,071,007 (GRCm39)	missense	probably damaging	1.00
R5061:Unc79	UTSW	12	103,134,700 (GRCm39)	missense	possibly damaging	0.94
R5075:Unc79	UTSW	12	103,041,213 (GRCm39)	missense	possibly damaging	0.63
R5101:Unc79	UTSW	12	103,078,769 (GRCm39)	missense	probably damaging	1.00
R5236:Unc79	UTSW	12	103,060,654 (GRCm39)	critical splice donor site	probably null
R5240:Unc79	UTSW	12	103,037,010 (GRCm39)	missense	probably damaging	0.99
R5383:Unc79	UTSW	12	103,070,886 (GRCm39)	missense	possibly damaging	0.53
R5461:Unc79	UTSW	12	103,078,397 (GRCm39)	missense	probably damaging	1.00
R5535:Unc79	UTSW	12	103,135,962 (GRCm39)	missense	possibly damaging	0.84
R5609:Unc79	UTSW	12	103,094,527 (GRCm39)	missense	probably benign
R5639:Unc79	UTSW	12	103,137,831 (GRCm39)	missense	probably damaging	1.00
R5704:Unc79	UTSW	12	102,968,202 (GRCm39)	missense	probably damaging	1.00
R5923:Unc79	UTSW	12	103,078,727 (GRCm39)	missense	probably damaging	1.00
R5925:Unc79	UTSW	12	103,091,989 (GRCm39)	splice site	probably null
R5975:Unc79	UTSW	12	103,091,885 (GRCm39)	missense	possibly damaging	0.53
R6047:Unc79	UTSW	12	103,027,717 (GRCm39)	missense	probably damaging	1.00
R6156:Unc79	UTSW	12	103,027,717 (GRCm39)	missense	probably damaging	1.00
R6175:Unc79	UTSW	12	103,149,708 (GRCm39)	missense	probably damaging	0.98
R6292:Unc79	UTSW	12	103,108,991 (GRCm39)	missense	possibly damaging	0.88
R6313:Unc79	UTSW	12	103,078,878 (GRCm39)	missense	probably damaging	1.00
R6391:Unc79	UTSW	12	102,987,269 (GRCm39)	missense	probably damaging	1.00
R6405:Unc79	UTSW	12	103,134,595 (GRCm39)	missense	probably damaging	0.97
R6416:Unc79	UTSW	12	103,097,905 (GRCm39)	missense	possibly damaging	0.86
R6467:Unc79	UTSW	12	103,139,771 (GRCm39)	missense	probably damaging	1.00
R6573:Unc79	UTSW	12	103,027,647 (GRCm39)	missense	probably damaging	1.00
R6614:Unc79	UTSW	12	102,957,689 (GRCm39)	missense	probably damaging	1.00
R6654:Unc79	UTSW	12	103,045,308 (GRCm39)	missense	probably damaging	1.00
R6654:Unc79	UTSW	12	103,045,307 (GRCm39)	missense	probably damaging	0.99
R6700:Unc79	UTSW	12	103,091,962 (GRCm39)	missense	possibly damaging	0.92
R6724:Unc79	UTSW	12	103,071,120 (GRCm39)	missense	probably damaging	1.00
R6819:Unc79	UTSW	12	103,108,267 (GRCm39)	missense	probably benign	0.12
R6869:Unc79	UTSW	12	103,079,331 (GRCm39)	missense	probably benign	0.33
R6879:Unc79	UTSW	12	103,115,046 (GRCm39)	splice site	probably null
R6942:Unc79	UTSW	12	103,088,704 (GRCm39)	critical splice donor site	probably null
R6961:Unc79	UTSW	12	103,079,174 (GRCm39)	missense	probably damaging	1.00
R6973:Unc79	UTSW	12	102,964,699 (GRCm39)	missense	possibly damaging	0.86
R6980:Unc79	UTSW	12	103,025,759 (GRCm39)	missense	probably damaging	1.00
R7124:Unc79	UTSW	12	103,027,652 (GRCm39)	missense	probably damaging	0.99
R7144:Unc79	UTSW	12	103,108,885 (GRCm39)	missense	probably benign	0.06
R7197:Unc79	UTSW	12	103,078,765 (GRCm39)	missense	probably benign
R7209:Unc79	UTSW	12	103,091,883 (GRCm39)	missense	probably benign
R7232:Unc79	UTSW	12	103,100,734 (GRCm39)	missense	possibly damaging	0.49
R7304:Unc79	UTSW	12	103,029,449 (GRCm39)	missense	probably damaging	1.00
R7354:Unc79	UTSW	12	103,108,961 (GRCm39)	missense	possibly damaging	0.79
R7384:Unc79	UTSW	12	103,137,837 (GRCm39)	missense	probably benign	0.11
R7400:Unc79	UTSW	12	103,070,889 (GRCm39)	missense	probably damaging	1.00
R7417:Unc79	UTSW	12	103,055,017 (GRCm39)	missense	possibly damaging	0.85
R7470:Unc79	UTSW	12	103,061,235 (GRCm39)	missense	probably damaging	1.00
R7842:Unc79	UTSW	12	103,058,313 (GRCm39)	missense	probably damaging	1.00
R8037:Unc79	UTSW	12	103,016,178 (GRCm39)	missense	probably damaging	1.00
R8041:Unc79	UTSW	12	103,054,726 (GRCm39)	missense	probably benign	0.06
R8146:Unc79	UTSW	12	103,036,416 (GRCm39)	missense	probably damaging	0.98
R8276:Unc79	UTSW	12	102,968,122 (GRCm39)	missense	possibly damaging	0.94
R8427:Unc79	UTSW	12	103,045,297 (GRCm39)	missense	probably benign	0.24
R8501:Unc79	UTSW	12	103,058,897 (GRCm39)	missense	probably damaging	1.00
R8510:Unc79	UTSW	12	103,070,898 (GRCm39)	missense	probably damaging	1.00
R8531:Unc79	UTSW	12	103,013,922 (GRCm39)	missense	probably damaging	1.00
R8531:Unc79	UTSW	12	103,049,855 (GRCm39)	missense	probably benign	0.13
R8795:Unc79	UTSW	12	103,074,513 (GRCm39)	missense	probably damaging	1.00
R9017:Unc79	UTSW	12	103,074,874 (GRCm39)	critical splice acceptor site	probably null
R9121:Unc79	UTSW	12	102,968,095 (GRCm39)	missense	probably damaging	1.00
R9196:Unc79	UTSW	12	103,078,613 (GRCm39)	missense	probably benign
R9443:Unc79	UTSW	12	103,037,035 (GRCm39)	missense	probably damaging	1.00
R9548:Unc79	UTSW	12	102,977,495 (GRCm39)	missense	probably damaging	1.00
R9600:Unc79	UTSW	12	103,135,972 (GRCm39)	missense	probably benign	0.07
R9767:Unc79	UTSW	12	103,079,234 (GRCm39)	missense	probably benign
R9787:Unc79	UTSW	12	103,112,620 (GRCm39)	missense	probably benign	0.00
RF010:Unc79	UTSW	12	103,079,046 (GRCm39)	missense	probably benign	0.17
X0017:Unc79	UTSW	12	103,074,520 (GRCm39)	missense	probably damaging	0.99
X0028:Unc79	UTSW	12	102,957,662 (GRCm39)	missense	probably damaging	1.00
Z1088:Unc79	UTSW	12	102,987,271 (GRCm39)	missense	probably damaging	1.00
Z1176:Unc79	UTSW	12	103,108,312 (GRCm39)	missense	probably benign	0.03
Z1176:Unc79	UTSW	12	103,054,937 (GRCm39)	missense	probably damaging	1.00
Z1177:Unc79	UTSW	12	103,131,948 (GRCm39)	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- TGCACTTAGCAGAAAGAACTGACCC -3'
(R):5'- AGAAAGCTTCGTTTGTGGCCCTC -3'

Sequencing Primer
(F):5'- GAAAGAACTGACCCGTCTACC -3'
(R):5'- GAGAGTACAGTACCAAGGTTCCC -3'

Posted On

2014-03-28