Mutagenetix > Incidental Mutation

Incidental Mutation 'R1146:Or10g9b'

165115

Institutional Source

Beutler Lab

Gene Symbol

Or10g9b

Ensembl Gene

ENSMUSG00000060254

Gene Name

olfactory receptor family 10 subfamily G member 9B

Synonyms

MOR223-2, GA_x6K02T2PVTD-33705428-33704496, Olfr980

MMRRC Submission

039219-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.073) question?

Stock #

R1146 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

39917290-39918325 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 39917390 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Aspartic acid at position 285 (V285D)

Ref Sequence

ENSEMBL: ENSMUSP00000150496 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000073932] [ENSMUST00000080835] [ENSMUST00000215523] [ENSMUST00000216463]

AlphaFold

Q8VH08

Predicted Effect

possibly damaging
Transcript: ENSMUST00000073932
AA Change: V285D

PolyPhen 2 Score 0.948 (Sensitivity: 0.79; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000091386
Gene: ENSMUSG00000060254
AA Change: V285D

Domain	Start	End	E-Value	Type
Pfam:7tm_4	29	304	1.4e-55	PFAM
Pfam:7tm_1	39	287	5.4e-23	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000080835

SMART Domains

Protein: ENSMUSP00000079648
Gene: ENSMUSG00000059473

Domain	Start	End	E-Value	Type
Pfam:7tm_4	29	304	7.9e-58	PFAM
Pfam:7tm_1	39	287	5.2e-22	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000215523

Predicted Effect

possibly damaging
Transcript: ENSMUST00000216463
AA Change: V285D

PolyPhen 2 Score 0.948 (Sensitivity: 0.79; Specificity: 0.95)

Meta Mutation Damage Score

0.3855

Coding Region Coverage

1x: 99.6%
3x: 98.5%
10x: 95.4%
20x: 88.7%

Validation Efficiency

98% (44/45)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 50 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adgrv1	A	T	13: 81,679,795 (GRCm39)	V1848E	probably damaging	Het
Alpk3	A	G	7: 80,727,343 (GRCm39)	K158E	probably damaging	Het
Arrdc4	T	A	7: 68,389,756 (GRCm39)	E356D	probably damaging	Het
Asb4	A	G	6: 5,423,591 (GRCm39)	N246S	probably damaging	Het
Ccdc150	A	T	1: 54,404,130 (GRCm39)		probably benign	Het
Ctsj	G	A	13: 61,150,312 (GRCm39)	P230L	probably benign	Het
Dync1i2	C	T	2: 71,058,164 (GRCm39)		probably benign	Het
Eme1	A	G	11: 94,536,277 (GRCm39)	L564P	probably damaging	Het
Fabp3	C	T	4: 130,206,180 (GRCm39)	T57I	probably benign	Het
Frg1	A	G	8: 41,864,254 (GRCm39)		probably benign	Het
Fzd2	A	T	11: 102,496,206 (GRCm39)	S217C	possibly damaging	Het
Gaa	G	T	11: 119,165,730 (GRCm39)	R81L	probably damaging	Het
Gfral	A	G	9: 76,074,341 (GRCm39)	V368A	probably benign	Het
Gm10774	T	C	2: 126,551,392 (GRCm39)	Y29C	probably benign	Het
Gucy1a2	T	A	9: 3,759,830 (GRCm39)	N545K	probably damaging	Het
Herc2	T	C	7: 55,796,444 (GRCm39)	S1939P	probably benign	Het
Ifnk	T	C	4: 35,152,231 (GRCm39)	I53T	probably benign	Het
Iqub	G	A	6: 24,505,627 (GRCm39)	L94F	possibly damaging	Het
Kpna1	C	T	16: 35,853,749 (GRCm39)	R460*	probably null	Het
Masp1	T	C	16: 23,310,865 (GRCm39)	E189G	probably damaging	Het
Mogat1	A	G	1: 78,500,250 (GRCm39)	I105V	probably benign	Het
Mpi	A	G	9: 57,452,472 (GRCm39)		probably benign	Het
Msh2	C	A	17: 87,987,488 (GRCm39)	D209E	probably benign	Het
Nsf	G	A	11: 103,719,364 (GRCm39)	T646I	probably damaging	Het
Or4c10b	C	T	2: 89,711,550 (GRCm39)	P127S	probably damaging	Het
Or5b24	T	A	19: 12,912,329 (GRCm39)	S76T	possibly damaging	Het
Or6b2	G	A	1: 92,407,612 (GRCm39)	H244Y	probably damaging	Het
Otogl	T	A	10: 107,722,374 (GRCm39)	I327F	probably damaging	Het
Pappa2	T	C	1: 158,682,552 (GRCm39)	D832G	probably damaging	Het
Pax5	G	T	4: 44,697,512 (GRCm39)		probably benign	Het
Pfkfb4	A	G	9: 108,836,794 (GRCm39)	E163G	probably benign	Het
Phc1	T	C	6: 122,300,416 (GRCm39)		probably benign	Het
Piwil1	T	C	5: 128,824,957 (GRCm39)	S552P	probably benign	Het
Ppfia3	A	C	7: 45,001,639 (GRCm39)	D424E	probably benign	Het
Prss16	A	G	13: 22,191,138 (GRCm39)		probably benign	Het
Ptpro	T	A	6: 137,420,592 (GRCm39)	V1007D	probably damaging	Het
Rbsn	A	G	6: 92,178,711 (GRCm39)		probably null	Het
Rexo1	C	T	10: 80,380,239 (GRCm39)	S919N	probably benign	Het
Sec31a	T	C	5: 100,510,032 (GRCm39)	N1152D	probably damaging	Het
Sel1l3	A	T	5: 53,274,445 (GRCm39)	F1012I	possibly damaging	Het
Sema4c	A	G	1: 36,589,646 (GRCm39)	V539A	probably benign	Het
Sf3b5	A	G	10: 12,884,575 (GRCm39)	E70G	possibly damaging	Het
Tmcc2	TTGCTGCTGCTGCTGCTGC	TTGCTGCTGCTGCTGC	1: 132,285,493 (GRCm39)		probably benign	Het
Tor1aip2	T	C	1: 155,940,483 (GRCm39)	V263A	possibly damaging	Het
Unc45b	A	G	11: 82,813,733 (GRCm39)	E380G	probably damaging	Het
Usp16	C	T	16: 87,271,536 (GRCm39)	T364M	possibly damaging	Het
Wwox	T	C	8: 115,438,776 (GRCm39)	S281P	probably damaging	Het
Zfp110	A	G	7: 12,580,721 (GRCm39)		probably null	Het
Zfp335	G	A	2: 164,738,043 (GRCm39)	A856V	probably benign	Het
Zfp652	G	A	11: 95,640,608 (GRCm39)	E178K	possibly damaging	Het

Other mutations in Or10g9b

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02256:Or10g9b	APN	9	39,917,349 (GRCm39)	missense	probably benign
IGL02378:Or10g9b	APN	9	39,917,769 (GRCm39)	missense	probably damaging	1.00
IGL03384:Or10g9b	APN	9	39,917,766 (GRCm39)	missense	probably benign
IGL03402:Or10g9b	APN	9	39,917,802 (GRCm39)	missense	probably benign	0.31
BB010:Or10g9b	UTSW	9	39,918,431 (GRCm39)	start gained	probably benign
PIT4651001:Or10g9b	UTSW	9	39,917,526 (GRCm39)	missense	probably damaging	0.97
R0013:Or10g9b	UTSW	9	39,917,651 (GRCm39)	missense	probably damaging	1.00
R1146:Or10g9b	UTSW	9	39,917,390 (GRCm39)	missense	possibly damaging	0.95
R4541:Or10g9b	UTSW	9	39,917,589 (GRCm39)	missense	possibly damaging	0.95
R4562:Or10g9b	UTSW	9	39,917,577 (GRCm39)	missense	probably damaging	0.99
R4731:Or10g9b	UTSW	9	39,917,564 (GRCm39)	missense	probably damaging	1.00
R4732:Or10g9b	UTSW	9	39,917,564 (GRCm39)	missense	probably damaging	1.00
R4733:Or10g9b	UTSW	9	39,917,564 (GRCm39)	missense	probably damaging	1.00
R4825:Or10g9b	UTSW	9	39,918,038 (GRCm39)	missense	possibly damaging	0.72
R5619:Or10g9b	UTSW	9	39,918,039 (GRCm39)	missense	probably benign	0.07
R5770:Or10g9b	UTSW	9	39,917,634 (GRCm39)	missense	probably benign	0.01
R5791:Or10g9b	UTSW	9	39,918,030 (GRCm39)	missense	probably damaging	1.00
R6813:Or10g9b	UTSW	9	39,917,753 (GRCm39)	missense	probably benign
R6819:Or10g9b	UTSW	9	39,917,844 (GRCm39)	missense	probably benign	0.00
R6970:Or10g9b	UTSW	9	39,918,009 (GRCm39)	missense	probably benign	0.00
R7490:Or10g9b	UTSW	9	39,917,720 (GRCm39)	missense	probably damaging	1.00
R7511:Or10g9b	UTSW	9	39,918,229 (GRCm39)	missense	possibly damaging	0.63
R7933:Or10g9b	UTSW	9	39,918,431 (GRCm39)	start gained	probably benign
R8243:Or10g9b	UTSW	9	39,917,484 (GRCm39)	missense	probably benign	0.19
Z1088:Or10g9b	UTSW	9	39,917,892 (GRCm39)	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- CCTCACACTGGATCTTGACCACATC -3'
(R):5'- TTTCCAAACATGTGCCTCACACTGC -3'

Sequencing Primer
(F):5'- TGGATCTTGACCACATCACAATG -3'
(R):5'- ACACTGCATCGTGGTCC -3'

Posted On

2014-03-28