Mutagenetix > Incidental Mutation

Incidental Mutation 'R1147:Vmn2r114'

165180

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r114

Ensembl Gene

ENSMUSG00000091945

Gene Name

vomeronasal 2, receptor 114

Synonyms

EG666002

MMRRC Submission

039220-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.142) question?

Stock #

R1147 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

23509908-23531287 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 23530037 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Histidine to Glutamine at position 123 (H123Q)

Ref Sequence

ENSEMBL: ENSMUSP00000127505 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000168033]

AlphaFold

E9Q281

Predicted Effect

probably benign
Transcript: ENSMUST00000168033
AA Change: H123Q

PolyPhen 2 Score 0.004 (Sensitivity: 0.98; Specificity: 0.59)

SMART Domains

Protein: ENSMUSP00000127505
Gene: ENSMUSG00000091945
AA Change: H123Q

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	154	470	1.5e-24	PFAM
Pfam:NCD3G	511	564	1.5e-18	PFAM
Pfam:7tm_3	597	832	1.4e-55	PFAM

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.2%
3x: 98.3%
10x: 96.0%
20x: 91.6%

Validation Efficiency

98% (47/48)

Allele List at MGI

Other mutations in this stock

Total: 47 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adam20	T	C	8: 41,248,655 (GRCm39)	I255T	possibly damaging	Het
Aknad1	T	A	3: 108,659,857 (GRCm39)	N290K	possibly damaging	Het
Ano8	C	A	8: 71,934,661 (GRCm39)	V447F	probably damaging	Het
Arhgef12	C	T	9: 42,955,552 (GRCm39)		probably benign	Het
Arsi	G	A	18: 61,049,723 (GRCm39)	G202E	probably benign	Het
Ash1l	A	T	3: 88,892,194 (GRCm39)	M1358L	possibly damaging	Het
Ccdc110	A	G	8: 46,397,121 (GRCm39)	K837E	possibly damaging	Het
Cd19	T	A	7: 126,010,217 (GRCm39)	D384V	possibly damaging	Het
Ces1f	C	T	8: 93,984,909 (GRCm39)	V473I	possibly damaging	Het
Chd6	C	T	2: 160,832,191 (GRCm39)	E994K	probably damaging	Het
Col5a2	G	T	1: 45,415,931 (GRCm39)	N1405K	probably damaging	Het
Dnah7b	A	G	1: 46,379,426 (GRCm39)	D3720G	probably damaging	Het
Dsel	T	C	1: 111,789,939 (GRCm39)	T199A	possibly damaging	Het
Fabp3	C	T	4: 130,206,180 (GRCm39)	T57I	probably benign	Het
Flacc1	A	T	1: 58,708,622 (GRCm39)	Y215N	probably damaging	Het
Hrg	G	T	16: 22,779,754 (GRCm39)	C344F	probably damaging	Het
Htt	T	C	5: 35,008,596 (GRCm39)	Y1462H	probably damaging	Het
Kcnh2	T	A	5: 24,529,385 (GRCm39)	I784F	probably damaging	Het
Kifc3	T	C	8: 95,864,546 (GRCm39)	T55A	probably damaging	Het
Kirrel1	C	T	3: 86,996,458 (GRCm39)	M380I	probably null	Het
Lsamp	G	A	16: 41,994,499 (GRCm39)		probably benign	Het
Meis3	G	A	7: 15,917,701 (GRCm39)		probably benign	Het
Nlrp4d	A	T	7: 10,122,644 (GRCm39)	N73K	probably benign	Het
Oog3	A	G	4: 143,884,982 (GRCm39)	F318S	possibly damaging	Het
Or2a20	A	T	6: 43,194,146 (GRCm39)	T100S	probably damaging	Het
Or52w1	G	A	7: 105,018,484 (GRCm39)	R308Q	probably benign	Het
Pde5a	C	T	3: 122,587,962 (GRCm39)	T376M	probably damaging	Het
Pkhd1l1	A	G	15: 44,400,837 (GRCm39)	I2204V	probably null	Het
Ppp1r13l	A	G	7: 19,109,772 (GRCm39)	D731G	probably damaging	Het
Prob1	G	A	18: 35,787,859 (GRCm39)	Q132*	probably null	Het
Ptk6	C	T	2: 180,837,590 (GRCm39)	G443D	probably benign	Het
Ptpro	T	A	6: 137,420,592 (GRCm39)	V1007D	probably damaging	Het
Ptprs	T	C	17: 56,730,504 (GRCm39)	D749G	probably damaging	Het
Ralgapa1	A	T	12: 55,749,265 (GRCm39)	D1212E	probably damaging	Het
Rsad1	T	C	11: 94,434,966 (GRCm39)	Y290C	probably damaging	Het
Scamp1	A	G	13: 94,361,394 (GRCm39)		probably null	Het
Slc6a11	T	A	6: 114,221,831 (GRCm39)	I507N	possibly damaging	Het
Stx18	T	G	5: 38,284,267 (GRCm39)		probably benign	Het
Sybu	A	T	15: 44,609,651 (GRCm39)	F78I	probably damaging	Het
Tecpr2	T	G	12: 110,907,872 (GRCm39)		probably benign	Het
Tox	A	T	4: 6,823,055 (GRCm39)	N87K	possibly damaging	Het
Trrap	G	A	5: 144,741,576 (GRCm39)	G1308R	probably damaging	Het
Trub2	A	G	2: 29,677,644 (GRCm39)		probably benign	Het
Vmn2r15	T	A	5: 109,441,072 (GRCm39)	Y262F	probably damaging	Het
Vmn2r33	C	T	7: 7,557,144 (GRCm39)	E519K	probably benign	Het
Zfat	A	T	15: 68,084,432 (GRCm39)		probably benign	Het
Zfp106	A	T	2: 120,351,017 (GRCm39)	C1545S	probably damaging	Het

Other mutations in Vmn2r114

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00157:Vmn2r114	APN	17	23,510,639 (GRCm39)	missense	probably damaging	1.00
IGL00990:Vmn2r114	APN	17	23,510,212 (GRCm39)	missense	probably damaging	1.00
IGL00990:Vmn2r114	APN	17	23,509,957 (GRCm39)	missense	probably benign	0.23
IGL00990:Vmn2r114	APN	17	23,509,939 (GRCm39)	missense	probably benign
IGL01838:Vmn2r114	APN	17	23,515,956 (GRCm39)	missense	probably benign	0.44
IGL01990:Vmn2r114	APN	17	23,529,355 (GRCm39)	missense	probably benign	0.22
IGL01994:Vmn2r114	APN	17	23,529,451 (GRCm39)	missense	probably damaging	1.00
IGL02153:Vmn2r114	APN	17	23,510,782 (GRCm39)	missense	probably benign	0.01
IGL02453:Vmn2r114	APN	17	23,530,108 (GRCm39)	missense	probably benign	0.00
IGL02621:Vmn2r114	APN	17	23,529,494 (GRCm39)	missense	probably damaging	0.98
IGL02938:Vmn2r114	APN	17	23,510,263 (GRCm39)	missense	probably benign	0.10
IGL03130:Vmn2r114	APN	17	23,515,970 (GRCm39)	splice site	probably benign
IGL03325:Vmn2r114	APN	17	23,510,652 (GRCm39)	missense	probably damaging	1.00
BB004:Vmn2r114	UTSW	17	23,510,619 (GRCm39)	missense	probably damaging	1.00
R0109:Vmn2r114	UTSW	17	23,529,549 (GRCm39)	nonsense	probably null
R0164:Vmn2r114	UTSW	17	23,528,800 (GRCm39)	critical splice donor site	probably null
R0310:Vmn2r114	UTSW	17	23,509,917 (GRCm39)	missense	probably benign	0.23
R0583:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R0677:Vmn2r114	UTSW	17	23,529,568 (GRCm39)	missense	probably damaging	1.00
R1127:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R1147:Vmn2r114	UTSW	17	23,530,037 (GRCm39)	missense	probably benign	0.00
R1157:Vmn2r114	UTSW	17	23,529,314 (GRCm39)	missense	possibly damaging	0.60
R1323:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R1347:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R1435:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R1437:Vmn2r114	UTSW	17	23,510,185 (GRCm39)	missense	probably damaging	1.00
R1585:Vmn2r114	UTSW	17	23,510,675 (GRCm39)	missense	probably damaging	0.98
R1641:Vmn2r114	UTSW	17	23,515,962 (GRCm39)	missense	probably benign	0.00
R1748:Vmn2r114	UTSW	17	23,527,035 (GRCm39)	missense	probably benign	0.17
R1954:Vmn2r114	UTSW	17	23,530,086 (GRCm39)	missense	probably benign	0.32
R2081:Vmn2r114	UTSW	17	23,510,083 (GRCm39)	missense	possibly damaging	0.91
R2103:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R2113:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R2134:Vmn2r114	UTSW	17	23,510,737 (GRCm39)	missense	probably damaging	1.00
R2149:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R2424:Vmn2r114	UTSW	17	23,515,842 (GRCm39)	missense	possibly damaging	0.90
R2847:Vmn2r114	UTSW	17	23,509,948 (GRCm39)	missense	probably benign	0.00
R2848:Vmn2r114	UTSW	17	23,509,948 (GRCm39)	missense	probably benign	0.00
R2893:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R3017:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R3018:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R3019:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R3020:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R3021:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R4628:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R4668:Vmn2r114	UTSW	17	23,529,447 (GRCm39)	missense	possibly damaging	0.83
R4840:Vmn2r114	UTSW	17	23,510,353 (GRCm39)	missense	probably damaging	0.97
R4841:Vmn2r114	UTSW	17	23,529,336 (GRCm39)	missense	probably benign	0.04
R4842:Vmn2r114	UTSW	17	23,529,336 (GRCm39)	missense	probably benign	0.04
R4856:Vmn2r114	UTSW	17	23,527,008 (GRCm39)	missense	probably benign	0.11
R4886:Vmn2r114	UTSW	17	23,527,008 (GRCm39)	missense	probably benign	0.11
R4992:Vmn2r114	UTSW	17	23,510,765 (GRCm39)	missense	probably benign	0.03
R5182:Vmn2r114	UTSW	17	23,510,632 (GRCm39)	missense	probably damaging	0.96
R5223:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R5405:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R5449:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R5615:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R5834:Vmn2r114	UTSW	17	23,529,599 (GRCm39)	missense	possibly damaging	0.90
R6150:Vmn2r114	UTSW	17	23,510,269 (GRCm39)	missense	probably benign	0.03
R6277:Vmn2r114	UTSW	17	23,509,954 (GRCm39)	missense	possibly damaging	0.93
R6403:Vmn2r114	UTSW	17	23,528,939 (GRCm39)	missense	probably damaging	0.99
R6589:Vmn2r114	UTSW	17	23,510,642 (GRCm39)	missense	probably damaging	1.00
R6613:Vmn2r114	UTSW	17	23,529,220 (GRCm39)	missense	possibly damaging	0.82
R6747:Vmn2r114	UTSW	17	23,528,850 (GRCm39)	missense	probably benign	0.00
R6837:Vmn2r114	UTSW	17	23,529,176 (GRCm39)	missense	probably benign	0.10
R6911:Vmn2r114	UTSW	17	23,510,104 (GRCm39)	missense	probably damaging	0.98
R6950:Vmn2r114	UTSW	17	23,529,137 (GRCm39)	missense	probably benign	0.03
R7276:Vmn2r114	UTSW	17	23,509,934 (GRCm39)	missense	probably damaging	0.97
R7482:Vmn2r114	UTSW	17	23,510,468 (GRCm39)	missense	probably damaging	1.00
R7514:Vmn2r114	UTSW	17	23,527,035 (GRCm39)	missense	probably null	0.96
R7523:Vmn2r114	UTSW	17	23,529,611 (GRCm39)	missense	probably benign	0.01
R7563:Vmn2r114	UTSW	17	23,510,000 (GRCm39)	missense	probably benign	0.01
R7585:Vmn2r114	UTSW	17	23,510,239 (GRCm39)	missense	probably damaging	1.00
R7593:Vmn2r114	UTSW	17	23,510,817 (GRCm39)	nonsense	probably null
R7611:Vmn2r114	UTSW	17	23,515,944 (GRCm39)	missense	probably damaging	0.97
R7641:Vmn2r114	UTSW	17	23,527,177 (GRCm39)	missense	possibly damaging	0.53
R7651:Vmn2r114	UTSW	17	23,509,986 (GRCm39)	nonsense	probably null
R7970:Vmn2r114	UTSW	17	23,530,186 (GRCm39)	missense	probably benign	0.00
R8737:Vmn2r114	UTSW	17	23,529,142 (GRCm39)	missense	probably benign	0.36
R8802:Vmn2r114	UTSW	17	23,528,836 (GRCm39)	missense	possibly damaging	0.65
R8847:Vmn2r114	UTSW	17	23,528,986 (GRCm39)	missense	probably damaging	1.00
R8991:Vmn2r114	UTSW	17	23,529,286 (GRCm39)	missense	probably damaging	1.00
R9138:Vmn2r114	UTSW	17	23,510,578 (GRCm39)	missense	probably damaging	1.00
R9173:Vmn2r114	UTSW	17	23,510,527 (GRCm39)	missense	probably damaging	0.99
R9175:Vmn2r114	UTSW	17	23,527,212 (GRCm39)	missense	probably damaging	1.00
R9657:Vmn2r114	UTSW	17	23,510,690 (GRCm39)	missense	probably damaging	1.00
R9670:Vmn2r114	UTSW	17	23,531,098 (GRCm39)	missense
X0065:Vmn2r114	UTSW	17	23,529,931 (GRCm39)	missense	probably benign	0.34
Z1088:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null

Predicted Primers

PCR Primer
(F):5'- TTAGATTTGCCCAGGCCAAGTAAGG -3'
(R):5'- ACAATGCAATCCAGTGGAGAATTGTCC -3'

Sequencing Primer
(F):5'- TAAATAAacacacaaacacacacac -3'
(R):5'- CAGTGGAGAATTGTCCTTTTATTGAC -3'

Posted On

2014-03-28