Mutagenetix > Incidental Mutation

Incidental Mutation 'R1473:Vmn2r6'

165901

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r6

Ensembl Gene

ENSMUSG00000090581

Gene Name

vomeronasal 2, receptor 6

Synonyms

EG620718, EG667069

MMRRC Submission

039526-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.171) question?

Stock #

R1473 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

64537561-64565298 bp(-) (GRCm38)

Type of Mutation

nonsense

DNA Base Change (assembly)

G to T at 64538158 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Stop codon at position 715 (Y715*)

Ref Sequence

ENSEMBL: ENSMUSP00000135148 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000165012] [ENSMUST00000176481]

AlphaFold

H3BK29

Predicted Effect

probably null
Transcript: ENSMUST00000165012
AA Change: Y626*

SMART Domains

Protein: ENSMUSP00000131831
Gene: ENSMUSG00000090581
AA Change: Y626*

Domain	Start	End	E-Value	Type
Pfam:ANF_receptor	1	416	1.4e-72	PFAM
Pfam:Peripla_BP_6	58	244	1.2e-10	PFAM
Pfam:NCD3G	458	511	1.8e-17	PFAM
Pfam:7tm_3	542	779	3.9e-76	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000176481
AA Change: Y715*

SMART Domains

Protein: ENSMUSP00000135148
Gene: ENSMUSG00000090581
AA Change: Y715*

Domain	Start	End	E-Value	Type
signal peptide	1	22	N/A	INTRINSIC
Pfam:ANF_receptor	88	505	9.8e-77	PFAM
Pfam:Peripla_BP_6	142	331	3.4e-10	PFAM
Pfam:NCD3G	547	600	5.4e-17	PFAM
Pfam:7tm_3	633	867	3.9e-47	PFAM

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 99.2%
3x: 98.4%
10x: 96.5%
20x: 93.2%

Validation Efficiency

98% (88/90)

Allele List at MGI

Other mutations in this stock

Total: 89 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ablim1	T	C	19: 57,068,236	D342G	probably damaging	Het
Acad8	T	C	9: 26,979,041	T293A	probably benign	Het
Adamts13	C	A	2: 26,981,753	Y310*	probably null	Het
Adcy6	T	A	15: 98,592,743	Y1102F	probably damaging	Het
Ahctf1	G	A	1: 179,776,108	T791M	probably benign	Het
Ahctf1	A	G	1: 179,799,279	V18A	probably damaging	Het
Ampd3	T	G	7: 110,804,935	S564R	probably damaging	Het
Anapc1	A	T	2: 128,617,697	I1814K	possibly damaging	Het
Arl4c	A	T	1: 88,701,609	L19Q	probably damaging	Het
Atp6v0e2	T	C	6: 48,539,264	Y49H	probably damaging	Het
Ccdc24	G	T	4: 117,869,904		probably benign	Het
Clcn6	A	C	4: 148,024,156	F139V	possibly damaging	Het
Col2a1	A	G	15: 97,982,908		probably benign	Het
Crip2	T	A	12: 113,143,500	C29S	probably damaging	Het
Cyp2a4	A	C	7: 26,314,763	N455T	probably benign	Het
Dhcr7	A	G	7: 143,841,368	D113G	probably damaging	Het
Dhcr7	T	C	7: 143,847,068	Y323H	probably damaging	Het
Dnah7a	A	C	1: 53,496,014	S2696A	probably benign	Het
Dnajc12	A	G	10: 63,397,244	T55A	probably benign	Het
Drosha	G	A	15: 12,912,520	E1075K	probably benign	Het
Duox2	A	G	2: 122,287,121	S911P	possibly damaging	Het
Ephb2	G	A	4: 136,694,058	A327V	possibly damaging	Het
Espl1	C	T	15: 102,320,443	T1711I	possibly damaging	Het
Fmnl2	A	G	2: 52,858,207	K22R	possibly damaging	Het
Fzd6	T	C	15: 39,030,963	F175L	probably damaging	Het
Gm4737	T	A	16: 46,154,819	E65V	probably damaging	Het
Gm5155	A	G	7: 17,905,091		noncoding transcript	Het
Gm6526	A	G	14: 43,748,846	I76M	probably damaging	Het
Gm6583	T	C	5: 112,354,549	T430A	probably benign	Het
Gm9881	A	T	16: 91,170,735	F34I	unknown	Het
Gm9892	T	C	8: 52,196,614	D148G	possibly damaging	Het
Grb10	C	T	11: 11,934,249	V486I	probably damaging	Het
Gtf3c3	C	T	1: 54,417,778	A488T	probably damaging	Het
Hdac4	T	C	1: 92,029,968	H108R	possibly damaging	Het
Hist2h2bb	G	T	3: 96,270,072	L107F	probably damaging	Het
Hmcn1	G	A	1: 150,772,552	T661I	probably benign	Het
Icam1	T	A	9: 21,027,876	I515N	probably damaging	Het
Ifi208	A	G	1: 173,695,654	R497G	possibly damaging	Het
Igsf10	A	T	3: 59,318,767	V2495E	probably damaging	Het
Iqgap1	T	C	7: 80,734,011	M1102V	probably benign	Het
Itgb4	A	T	11: 115,984,047	N410I	probably benign	Het
Jup	T	C	11: 100,379,601	H360R	possibly damaging	Het
Kif20b	T	A	19: 34,974,496	S1685T	possibly damaging	Het
Lins1	T	C	7: 66,712,046		probably null	Het
Lrig1	T	A	6: 94,607,313	T917S	probably benign	Het
Mast2	A	G	4: 116,311,955	S814P	probably damaging	Het
Mast4	T	C	13: 102,772,519	T483A	probably damaging	Het
Mcpt1	T	C	14: 56,019,533	M176T	probably benign	Het
Mettl22	A	G	16: 8,473,961	Q38R	probably damaging	Het
Mrm2	T	C	5: 140,328,688	T131A	probably benign	Het
Nde1	T	G	16: 14,185,864	F71V	probably benign	Het
Nxn	C	T	11: 76,263,187	G274D	possibly damaging	Het
Olfr1230	A	T	2: 89,296,906	Y121*	probably null	Het
Olfr183	A	T	16: 58,999,912	T76S	probably benign	Het
Olfr414	T	A	1: 174,430,643	W72R	probably damaging	Het
Olfr427	G	T	1: 174,099,749	C97F	probably damaging	Het
Osbp2	T	C	11: 3,717,175		probably null	Het
Otud7a	C	A	7: 63,754,629		probably benign	Het
Phf3	G	A	1: 30,805,940	L1313F	probably damaging	Het
Pkhd1	A	T	1: 20,522,983	D1635E	probably benign	Het
Plpp1	A	G	13: 112,859,664	H171R	probably damaging	Het
Pofut1	A	G	2: 153,261,246	M172V	probably damaging	Het
Prmt5	A	G	14: 54,508,915	F580L	probably damaging	Het
Rab11fip3	A	T	17: 25,991,322	L987Q	probably damaging	Het
Retnlb	T	A	16: 48,818,665	C76*	probably null	Het
Rnf38	T	C	4: 44,131,584	N399S	probably benign	Het
Sbk1	A	G	7: 126,292,252	E286G	possibly damaging	Het
Scin	T	A	12: 40,077,502	T430S	probably benign	Het
Sgsm1	T	A	5: 113,263,257	T868S	probably benign	Het
Sipa1l1	G	A	12: 82,341,111	R37H	probably damaging	Het
Smchd1	A	T	17: 71,361,837		probably benign	Het
Sned1	G	A	1: 93,281,654	V830M	possibly damaging	Het
Soga1	A	T	2: 157,020,448	Y1520*	probably null	Het
Stk32c	C	T	7: 139,125,179	R23Q	probably damaging	Het
Sult1d1	A	G	5: 87,564,739	M82T	probably benign	Het
Tat	T	C	8: 109,996,918	L346P	probably damaging	Het
Tenm3	C	A	8: 48,310,625	G789V	probably damaging	Het
Thsd7a	C	T	6: 12,338,622	S1203N	probably benign	Het
Tmem191c	G	A	16: 17,277,962		probably null	Het
Tmem268	A	G	4: 63,580,338	T239A	probably damaging	Het
Tmem82	A	C	4: 141,616,278	L227R	possibly damaging	Het
Ttn	A	T	2: 76,727,032	I29906N	probably damaging	Het
Txndc15	T	C	13: 55,721,574		probably benign	Het
Ubqln4	A	G	3: 88,565,845	I536V	probably benign	Het
Unc80	C	T	1: 66,521,581	H823Y	possibly damaging	Het
Vmn2r102	A	T	17: 19,694,581	I803F	probably benign	Het
Vmn2r74	A	T	7: 85,961,410	C25S	probably damaging	Het
Wdr38	A	G	2: 39,000,979	T261A	probably benign	Het
Zfp653	T	C	9: 22,058,220	E250G	possibly damaging	Het

Other mutations in Vmn2r6

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01547:Vmn2r6	APN	3	64538104	missense	probably damaging	1.00
IGL01968:Vmn2r6	APN	3	64556345	missense	possibly damaging	0.94
IGL02009:Vmn2r6	APN	3	64537902	missense	possibly damaging	0.61
IGL02039:Vmn2r6	APN	3	64556189	missense	probably damaging	1.00
IGL02652:Vmn2r6	APN	3	64556328	missense	probably benign	0.24
IGL02737:Vmn2r6	APN	3	64556490	missense	possibly damaging	0.55
IGL02808:Vmn2r6	APN	3	64556496	missense	probably damaging	1.00
IGL03066:Vmn2r6	APN	3	64565153	missense	probably damaging	0.99
IGL03331:Vmn2r6	APN	3	64538007	missense	probably damaging	1.00
BB010:Vmn2r6	UTSW	3	64559803	missense	probably benign	0.02
BB020:Vmn2r6	UTSW	3	64559803	missense	probably benign	0.02
R0010:Vmn2r6	UTSW	3	64559545	nonsense	probably null
R0206:Vmn2r6	UTSW	3	64539912	missense	probably benign
R0206:Vmn2r6	UTSW	3	64539912	missense	probably benign
R0208:Vmn2r6	UTSW	3	64539912	missense	probably benign
R0427:Vmn2r6	UTSW	3	64559587	missense	probably damaging	1.00
R0466:Vmn2r6	UTSW	3	64556302	missense	probably damaging	1.00
R1018:Vmn2r6	UTSW	3	64556840	missense	probably benign	0.00
R1104:Vmn2r6	UTSW	3	64538066	missense	possibly damaging	0.93
R1186:Vmn2r6	UTSW	3	64565067	missense	probably benign	0.01
R1245:Vmn2r6	UTSW	3	64556790	missense	possibly damaging	0.53
R1295:Vmn2r6	UTSW	3	64538273	missense	probably damaging	1.00
R1498:Vmn2r6	UTSW	3	64556469	missense	probably damaging	1.00
R1925:Vmn2r6	UTSW	3	64556277	missense	possibly damaging	0.87
R2044:Vmn2r6	UTSW	3	64537841	missense	probably damaging	0.96
R2069:Vmn2r6	UTSW	3	64556098	missense	possibly damaging	0.89
R2253:Vmn2r6	UTSW	3	64559718	missense	probably damaging	1.00
R2261:Vmn2r6	UTSW	3	64556669	missense	probably benign	0.24
R2262:Vmn2r6	UTSW	3	64556669	missense	probably benign	0.24
R2350:Vmn2r6	UTSW	3	64556352	missense	probably benign	0.01
R2680:Vmn2r6	UTSW	3	64538286	missense	possibly damaging	0.91
R2846:Vmn2r6	UTSW	3	64556790	missense	possibly damaging	0.53
R2860:Vmn2r6	UTSW	3	64547339	missense	probably benign	0.00
R2861:Vmn2r6	UTSW	3	64547339	missense	probably benign	0.00
R3766:Vmn2r6	UTSW	3	64556508	missense	probably benign	0.19
R3870:Vmn2r6	UTSW	3	64556621	missense	probably damaging	0.96
R4018:Vmn2r6	UTSW	3	64556472	missense	probably benign	0.05
R4024:Vmn2r6	UTSW	3	64538250	missense	possibly damaging	0.73
R4026:Vmn2r6	UTSW	3	64538250	missense	possibly damaging	0.73
R4227:Vmn2r6	UTSW	3	64537948	missense	probably damaging	0.99
R4526:Vmn2r6	UTSW	3	64537724	missense	probably benign	0.32
R4570:Vmn2r6	UTSW	3	64559647	missense	probably benign	0.31
R4894:Vmn2r6	UTSW	3	64547408	missense	probably benign
R4934:Vmn2r6	UTSW	3	64556345	missense	probably damaging	0.99
R5057:Vmn2r6	UTSW	3	64537786	missense	probably damaging	1.00
R5059:Vmn2r6	UTSW	3	64537623	missense	possibly damaging	0.89
R5148:Vmn2r6	UTSW	3	64556594	missense	probably damaging	0.99
R5155:Vmn2r6	UTSW	3	64538514	missense	probably benign	0.44
R5179:Vmn2r6	UTSW	3	64537990	missense	probably benign	0.00
R5256:Vmn2r6	UTSW	3	64556842	missense	probably benign	0.33
R5861:Vmn2r6	UTSW	3	64556033	missense	probably benign	0.00
R5950:Vmn2r6	UTSW	3	64565231	missense	probably benign	0.05
R6081:Vmn2r6	UTSW	3	64556532	missense	probably benign	0.25
R6173:Vmn2r6	UTSW	3	64559755	missense	probably damaging	1.00
R6190:Vmn2r6	UTSW	3	64538003	missense	probably benign	0.04
R6240:Vmn2r6	UTSW	3	64556805	missense	probably damaging	1.00
R6433:Vmn2r6	UTSW	3	64547380	nonsense	probably null
R6645:Vmn2r6	UTSW	3	64556876	missense	probably damaging	1.00
R6791:Vmn2r6	UTSW	3	64538159	missense	probably damaging	1.00
R7265:Vmn2r6	UTSW	3	64556774	missense	probably benign	0.00
R7503:Vmn2r6	UTSW	3	64539951	nonsense	probably null
R7562:Vmn2r6	UTSW	3	64556520	missense	probably benign	0.00
R7584:Vmn2r6	UTSW	3	64565262	missense	probably benign	0.07
R7611:Vmn2r6	UTSW	3	64565142	missense	probably damaging	0.98
R7759:Vmn2r6	UTSW	3	64556570	missense	probably damaging	1.00
R7834:Vmn2r6	UTSW	3	64538022	missense	probably damaging	1.00
R7933:Vmn2r6	UTSW	3	64559803	missense	probably benign	0.02
R7982:Vmn2r6	UTSW	3	64559820	missense	probably damaging	1.00
R8024:Vmn2r6	UTSW	3	64559824	missense	probably benign	0.40
R8074:Vmn2r6	UTSW	3	64547643	intron	probably benign
R8169:Vmn2r6	UTSW	3	64539889	missense	probably benign	0.01
R8337:Vmn2r6	UTSW	3	64556105	nonsense	probably null
R8736:Vmn2r6	UTSW	3	64559800	missense	probably damaging	1.00
R8962:Vmn2r6	UTSW	3	64556155	missense	probably damaging	1.00
R9139:Vmn2r6	UTSW	3	64556856	missense	probably benign	0.12
R9206:Vmn2r6	UTSW	3	64559611	missense	probably damaging	0.97
R9295:Vmn2r6	UTSW	3	64556063	missense	probably benign	0.00
R9332:Vmn2r6	UTSW	3	64547250	missense	probably benign	0.01
R9616:Vmn2r6	UTSW	3	64538303	missense	probably damaging	1.00
R9663:Vmn2r6	UTSW	3	64556128	missense	possibly damaging	0.90
R9685:Vmn2r6	UTSW	3	64556660	missense	probably benign	0.19
X0020:Vmn2r6	UTSW	3	64538450	missense	probably benign
X0066:Vmn2r6	UTSW	3	64547378	missense	probably damaging	1.00
Z1176:Vmn2r6	UTSW	3	64556325	missense	probably damaging	0.98

Predicted Primers

PCR Primer
(F):5'- TTTCCACAGCCACATTGAATTTGCC -3'
(R):5'- GAGCTGAGCTTCCTCATTCAGGTG -3'

Sequencing Primer
(F):5'- CTTTGGTGCCCAAGTAAGCAG -3'
(R):5'- GTCTCTGGGAATCACAGTGCTATC -3'

Posted On

2014-03-28