Mutagenetix > Incidental Mutation

Incidental Mutation 'R1466:Muc4'

166097

Institutional Source

Beutler Lab

Gene Symbol

Muc4

Ensembl Gene

ENSMUSG00000079620

Gene Name

mucin 4

Synonyms

4933405I11Rik

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.079) question?

Stock #

R1466 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

32735886-32782391 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 32753595 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Glycine to Aspartic acid at position 1157 (G1157D)

Ref Sequence

ENSEMBL: ENSMUSP00000093813 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000096106] [ENSMUST00000132475]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000096106
AA Change: G1157D

PolyPhen 2 Score 0.044 (Sensitivity: 0.94; Specificity: 0.83)

SMART Domains

Protein: ENSMUSP00000093813
Gene: ENSMUSG00000079620
AA Change: G1157D

Domain	Start	End	E-Value	Type
signal peptide	1	28	N/A	INTRINSIC
low complexity region	37	65	N/A	INTRINSIC
low complexity region	86	111	N/A	INTRINSIC
internal_repeat_2	119	903	6.07e-127	PROSPERO
internal_repeat_1	164	987	3.47e-144	PROSPERO
internal_repeat_2	979	1875	6.07e-127	PROSPERO
internal_repeat_1	1193	2087	3.47e-144	PROSPERO
low complexity region	2090	2106	N/A	INTRINSIC
low complexity region	2111	2119	N/A	INTRINSIC
low complexity region	2186	2195	N/A	INTRINSIC
low complexity region	2230	2249	N/A	INTRINSIC
low complexity region	2324	2332	N/A	INTRINSIC
low complexity region	2344	2367	N/A	INTRINSIC
NIDO	2458	2615	8.33e-67	SMART
AMOP	2614	2726	1.29e-47	SMART
VWD	2729	2910	4.23e-26	SMART
EGF_like	3134	3166	3.23e1	SMART
EGF_like	3176	3212	3.5e1	SMART
low complexity region	3237	3251	N/A	INTRINSIC
EGF	3384	3421	1.4e0	SMART
transmembrane domain	3430	3452	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000132475

SMART Domains

Protein: ENSMUSP00000119029
Gene: ENSMUSG00000079620

Domain	Start	End	E-Value	Type
low complexity region	38	45	N/A	INTRINSIC
low complexity region	72	84	N/A	INTRINSIC
low complexity region	99	127	N/A	INTRINSIC
low complexity region	148	173	N/A	INTRINSIC
low complexity region	192	224	N/A	INTRINSIC
low complexity region	283	293	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000135753

SMART Domains

Protein: ENSMUSP00000119154
Gene: ENSMUSG00000079620

Domain	Start	End	E-Value	Type
low complexity region	21	37	N/A	INTRINSIC
low complexity region	42	50	N/A	INTRINSIC
low complexity region	117	126	N/A	INTRINSIC
low complexity region	161	180	N/A	INTRINSIC
low complexity region	255	263	N/A	INTRINSIC
low complexity region	275	298	N/A	INTRINSIC
NIDO	389	546	8.33e-67	SMART
AMOP	545	657	1.29e-47	SMART
VWD	660	841	4.23e-26	SMART
EGF_like	1065	1097	3.23e1	SMART
EGF_like	1107	1143	3.5e1	SMART
low complexity region	1168	1182	N/A	INTRINSIC
EGF	1315	1352	1.4e0	SMART
transmembrane domain	1361	1383	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000142355

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.4%
3x: 97.9%
10x: 90.8%
20x: 71.5%

Validation Efficiency

MGI Phenotype

FUNCTION: The major constituents of mucus, the viscous secretion that covers epithelial surfaces such as those in the trachea, colon, and cervix, are highly glycosylated proteins called mucins. These glycoproteins play important roles in the protection of the epithelial cells and have been implicated in epithelial renewal and differentiation. This gene encodes an integral membrane glycoprotein found on the cell surface. A large 5' exon encodes at least 15 tandem repeats of 124-126 amino acids. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit resistance to DSS-treated colitis and colitis-associated colorectal cancer. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 108 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abhd15	C	T	11: 77,515,410 (GRCm38)	A71V	probably damaging	Het
Adamts12	T	C	15: 11,311,359 (GRCm38)	F1234S	probably benign	Het
Ahnak	A	G	19: 9,015,875 (GRCm38)	D4841G	probably damaging	Het
Akap13	T	C	7: 75,729,049 (GRCm38)	S2095P	possibly damaging	Het
Ampd2	T	C	3: 108,080,337 (GRCm38)		probably null	Het
Arhgef17	G	T	7: 100,929,659 (GRCm38)	P694Q	possibly damaging	Het
Arrdc1	T	C	2: 24,925,795 (GRCm38)	I398V	probably benign	Het
Ash1l	T	C	3: 89,052,065 (GRCm38)	Y2250H	probably damaging	Het
Aspg	A	G	12: 112,121,852 (GRCm38)	N385D	probably benign	Het
Atxn3	G	A	12: 101,926,499 (GRCm38)	R319C	possibly damaging	Het
Brca2	G	A	5: 150,552,258 (GRCm38)	A2478T	probably damaging	Het
C1s1	C	T	6: 124,531,131 (GRCm38)	C633Y	probably damaging	Het
C8g	C	T	2: 25,500,216 (GRCm38)	A6T	probably benign	Het
Cbl	A	T	9: 44,154,244 (GRCm38)	V706E	probably benign	Het
Ccdc121rt3	C	T	5: 112,354,764 (GRCm38)	G358D	probably benign	Het
Cfhr1	C	T	1: 139,557,574 (GRCm38)	E45K	probably benign	Het
Chd7	G	A	4: 8,840,561 (GRCm38)		probably null	Het
Chek1	G	T	9: 36,725,857 (GRCm38)	A2E	probably damaging	Het
Cntnap1	C	A	11: 101,180,360 (GRCm38)	F366L	probably damaging	Het
Corin	C	T	5: 72,302,790 (GRCm38)		probably null	Het
Crb2	T	C	2: 37,783,388 (GRCm38)	Y99H	probably damaging	Het
Ctdspl2	G	A	2: 122,003,929 (GRCm38)	R332K	probably benign	Het
Cym	G	A	3: 107,213,458 (GRCm38)	T277I	probably damaging	Het
Cyp2d11	C	T	15: 82,391,735 (GRCm38)	C215Y	probably benign	Het
Dido1	G	A	2: 180,662,328 (GRCm38)	P1261L	probably damaging	Het
Dnah10	T	A	5: 124,763,096 (GRCm38)	Y1265N	probably benign	Het
Dtx3l	G	A	16: 35,932,728 (GRCm38)	L503F	probably damaging	Het
Efhb	A	G	17: 53,437,178 (GRCm38)	F462L	probably damaging	Het
Enpep	T	A	3: 129,319,448 (GRCm38)	T203S	probably damaging	Het
Fat3	A	C	9: 16,375,482 (GRCm38)	V915G	probably damaging	Het
Fbln7	A	G	2: 128,877,429 (GRCm38)	T49A	probably benign	Het
Fcho1	C	T	8: 71,712,560 (GRCm38)	A418T	probably benign	Het
Fgf14	T	A	14: 124,676,539 (GRCm38)	K60M	probably benign	Het
Galnt4	A	G	10: 99,108,709 (GRCm38)	R99G	probably benign	Het
Gimap4	C	A	6: 48,691,282 (GRCm38)	Q196K	probably benign	Het
Glcci1	C	T	6: 8,537,964 (GRCm38)	T6I	probably damaging	Het
Gm10110	A	T	14: 89,898,075 (GRCm38)		noncoding transcript	Het
Gm4884	A	G	7: 41,043,128 (GRCm38)	K174E	probably damaging	Het
Grip2	A	T	6: 91,788,443 (GRCm38)	D19E	probably damaging	Het
Grk4	T	C	5: 34,694,750 (GRCm38)	S113P	probably benign	Het
Hectd3	G	A	4: 116,996,566 (GRCm38)	E220K	probably damaging	Het
Helz2	G	A	2: 181,236,297 (GRCm38)	P903S	probably damaging	Het
Hydin	T	A	8: 110,532,953 (GRCm38)	V2519E	possibly damaging	Het
Ints11	G	A	4: 155,888,110 (GRCm38)		probably null	Het
Kif1a	A	G	1: 93,054,929 (GRCm38)	W718R	possibly damaging	Het
Kif1b	A	T	4: 149,223,252 (GRCm38)	Y839N	probably damaging	Het
Kif20b	T	A	19: 34,950,599 (GRCm38)	V1047D	probably benign	Het
Klhl23	T	C	2: 69,833,888 (GRCm38)	I527T	probably damaging	Het
Klra10	T	A	6: 130,279,315 (GRCm38)	R125S	probably damaging	Het
Klra10	T	C	6: 130,279,431 (GRCm38)	N87D	probably damaging	Het
Lars1	G	A	18: 42,210,050 (GRCm38)	R1101C	probably damaging	Het
Lcn4	G	A	2: 26,668,576 (GRCm38)	P166L	probably damaging	Het
Letmd1	T	A	15: 100,472,542 (GRCm38)		probably null	Het
Map4k2	G	T	19: 6,341,917 (GRCm38)	W87L	probably damaging	Het
Mccc1	A	G	3: 35,974,286 (GRCm38)	V457A	probably benign	Het
Mdn1	T	A	4: 32,730,788 (GRCm38)	S2886T	probably benign	Het
Mroh2b	G	T	15: 4,925,684 (GRCm38)	D720Y	probably damaging	Het
Mrpl24	T	A	3: 87,921,928 (GRCm38)	Y21*	probably null	Het
Mrps35	C	T	6: 147,055,984 (GRCm38)	T169M	probably damaging	Het
Mtcl1	T	A	17: 66,380,435 (GRCm38)	D492V	probably damaging	Het
Muc2	A	G	7: 141,748,974 (GRCm38)	Y457C	probably damaging	Het
Myg1	T	A	15: 102,337,390 (GRCm38)	L275Q	probably damaging	Het
Naga	T	A	15: 82,334,788 (GRCm38)	M237L	probably null	Het
Oc90	T	A	15: 65,897,720 (GRCm38)	Y96F	probably damaging	Het
Or12d13	A	T	17: 37,336,956 (GRCm38)	L92H	probably benign	Het
Or13a18	A	G	7: 140,610,969 (GRCm38)	I268V	probably benign	Het
Or4a80	C	T	2: 89,752,267 (GRCm38)	C187Y	probably damaging	Het
Or5m11b	T	A	2: 85,975,995 (GRCm38)	F251I	probably damaging	Het
Or6ae1	A	G	7: 140,162,203 (GRCm38)	V249A	probably damaging	Het
Orc4	A	T	2: 48,909,494 (GRCm38)	C324S	possibly damaging	Het
Pcdh10	T	G	3: 45,379,974 (GRCm38)	L241R	probably damaging	Het
Pdzrn4	T	C	15: 92,770,537 (GRCm38)	S857P	probably benign	Het
Plec	C	T	15: 76,185,908 (GRCm38)	E1000K	possibly damaging	Het
Plvap	A	T	8: 71,508,481 (GRCm38)	V149D	probably benign	Het
Ppef1	C	A	X: 160,625,674 (GRCm38)		probably null	Het
Prkaa1	C	A	15: 5,178,798 (GRCm38)	P507T	probably benign	Het
Ptch1	A	G	13: 63,524,969 (GRCm38)	Y804H	probably benign	Het
R3hdm2	A	G	10: 127,476,690 (GRCm38)	I434V	probably benign	Het
Rfx5	T	A	3: 94,956,303 (GRCm38)	Y88N	probably damaging	Het
Rnase2b	A	T	14: 51,162,839 (GRCm38)	K126*	probably null	Het
Rpl3l	A	G	17: 24,730,871 (GRCm38)	I15V	probably benign	Het
Saal1	G	T	7: 46,702,545 (GRCm38)		probably null	Het
Sbpl	A	C	17: 23,953,254 (GRCm38)	D230E	unknown	Het
Scn10a	T	C	9: 119,666,490 (GRCm38)	Y322C	probably damaging	Het
Sec16a	A	T	2: 26,431,157 (GRCm38)	Y1308N	probably damaging	Het
Sis	A	T	3: 72,932,060 (GRCm38)	D824E	possibly damaging	Het
Slc25a36	A	G	9: 97,080,355 (GRCm38)	F194L	probably damaging	Het
Slc27a4	T	A	2: 29,811,190 (GRCm38)	V331E	probably damaging	Het
Slc7a11	G	T	3: 50,381,073 (GRCm38)		probably null	Het
Slco4c1	A	T	1: 96,841,172 (GRCm38)	S322T	probably damaging	Het
Smarcc2	A	T	10: 128,474,245 (GRCm38)	T376S	probably damaging	Het
Srebf1	C	T	11: 60,200,702 (GRCm38)	R999H	probably benign	Het
St3gal3	A	C	4: 118,107,662 (GRCm38)	M1R	probably null	Het
Syp	A	T	X: 7,648,705 (GRCm38)		probably benign	Het
Tas1r2	A	G	4: 139,669,411 (GRCm38)	D687G	probably damaging	Het
Tekt4	A	T	17: 25,472,074 (GRCm38)	Q118L	probably benign	Het
Thoc2l	T	A	5: 104,518,257 (GRCm38)	I215N	probably damaging	Het
Tph2	T	C	10: 115,079,695 (GRCm38)	N480S	probably benign	Het
Tsc2	A	T	17: 24,608,973 (GRCm38)	M839K	probably damaging	Het
Ttc22	T	C	4: 106,622,780 (GRCm38)	F77S	probably damaging	Het
Uaca	C	T	9: 60,854,321 (GRCm38)	A205V	possibly damaging	Het
Uhmk1	A	T	1: 170,208,653 (GRCm38)		probably null	Het
Usp17lc	A	G	7: 103,418,941 (GRCm38)	H481R	possibly damaging	Het
Vwa3a	A	G	7: 120,768,165 (GRCm38)	Y181C	probably damaging	Het
Wfikkn2	G	A	11: 94,238,895 (GRCm38)	T140I	probably damaging	Het
Zfp704	G	T	3: 9,447,348 (GRCm38)	T288N	possibly damaging	Het
Zfp93	T	C	7: 24,276,096 (GRCm38)	V502A	probably damaging	Het
Zzef1	C	T	11: 72,924,679 (GRCm38)	P2942S	probably damaging	Het

Other mutations in Muc4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00087:Muc4	APN	16	32,754,086 (GRCm38)	missense	probably benign	0.35
IGL00088:Muc4	APN	16	32,754,086 (GRCm38)	missense	probably benign	0.35
IGL00089:Muc4	APN	16	32,754,086 (GRCm38)	missense	probably benign	0.35
IGL00090:Muc4	APN	16	32,754,086 (GRCm38)	missense	probably benign	0.35
IGL00091:Muc4	APN	16	32,754,086 (GRCm38)	missense	probably benign	0.35
IGL00092:Muc4	APN	16	32,754,086 (GRCm38)	missense	probably benign	0.35
IGL00163:Muc4	APN	16	32,754,090 (GRCm38)	missense	probably benign	0.20
IGL00324:Muc4	APN	16	32,778,812 (GRCm38)	missense	probably benign	0.03
IGL00331:Muc4	APN	16	32,753,185 (GRCm38)	missense	probably benign	0.01
IGL00539:Muc4	APN	16	32,750,910 (GRCm38)	missense	possibly damaging	0.53
IGL00590:Muc4	APN	16	32,754,347 (GRCm38)	missense	probably benign	0.10
IGL00990:Muc4	APN	16	32,753,886 (GRCm38)	missense	probably benign	0.01
IGL00990:Muc4	APN	16	32,753,863 (GRCm38)	missense	probably benign	0.01
IGL00990:Muc4	APN	16	32,752,569 (GRCm38)	missense	probably benign	0.01
IGL00990:Muc4	APN	16	32,753,849 (GRCm38)	missense	probably benign	0.01
IGL00990:Muc4	APN	16	32,753,848 (GRCm38)	missense	probably benign	0.01
IGL00990:Muc4	APN	16	32,753,823 (GRCm38)	missense	probably benign	0.01
IGL00990:Muc4	APN	16	32,755,805 (GRCm38)	unclassified	probably benign
IGL00990:Muc4	APN	16	32,753,955 (GRCm38)	missense	possibly damaging	0.86
IGL00990:Muc4	APN	16	32,754,071 (GRCm38)	missense	probably benign	0.01
IGL01091:Muc4	APN	16	32,753,927 (GRCm38)	missense	probably benign	0.04
IGL01124:Muc4	APN	16	32,768,730 (GRCm38)	missense	possibly damaging	0.65
IGL01131:Muc4	APN	16	32,753,901 (GRCm38)	missense	possibly damaging	0.72
IGL01536:Muc4	APN	16	32,763,966 (GRCm38)	missense	possibly damaging	0.93
IGL01603:Muc4	APN	16	32,750,655 (GRCm38)	missense	probably benign	0.23
IGL01618:Muc4	APN	16	32,756,627 (GRCm38)	missense	unknown
IGL01625:Muc4	APN	16	32,755,544 (GRCm38)	unclassified	probably benign
IGL01626:Muc4	APN	16	32,736,402 (GRCm38)	missense	possibly damaging	0.48
IGL01653:Muc4	APN	16	32,761,348 (GRCm38)	splice site	probably null
IGL01682:Muc4	APN	16	32,754,086 (GRCm38)	missense	probably benign	0.35
IGL01870:Muc4	APN	16	32,753,196 (GRCm38)	missense	probably benign	0.01
IGL01966:Muc4	APN	16	32,751,426 (GRCm38)	missense	possibly damaging	0.84
IGL01973:Muc4	APN	16	32,754,265 (GRCm38)	missense	probably benign	0.01
IGL02089:Muc4	APN	16	32,751,313 (GRCm38)	missense	possibly damaging	0.83
IGL02152:Muc4	APN	16	32,777,649 (GRCm38)	splice site	probably benign
IGL02210:Muc4	APN	16	32,752,254 (GRCm38)	missense	probably benign	0.00
IGL02278:Muc4	APN	16	32,754,529 (GRCm38)	missense	probably benign	0.01
IGL02280:Muc4	APN	16	32,754,529 (GRCm38)	missense	probably benign	0.01
IGL02316:Muc4	APN	16	32,750,850 (GRCm38)	missense	possibly damaging	0.73
IGL02351:Muc4	APN	16	32,750,986 (GRCm38)	missense	possibly damaging	0.86
IGL02358:Muc4	APN	16	32,750,986 (GRCm38)	missense	possibly damaging	0.86
IGL02391:Muc4	APN	16	32,752,076 (GRCm38)	missense	probably benign	0.41
IGL02449:Muc4	APN	16	32,756,129 (GRCm38)	unclassified	probably benign
IGL02607:Muc4	APN	16	32,775,819 (GRCm38)	missense	possibly damaging	0.73
IGL02888:Muc4	APN	16	32,755,282 (GRCm38)	unclassified	probably benign
IGL02893:Muc4	APN	16	32,751,648 (GRCm38)	missense	possibly damaging	0.68
IGL02902:Muc4	APN	16	32,750,394 (GRCm38)	missense	possibly damaging	0.50
IGL03007:Muc4	APN	16	32,752,048 (GRCm38)	missense	possibly damaging	0.84
IGL03161:Muc4	APN	16	32,751,948 (GRCm38)	missense	possibly damaging	0.84
IGL03304:Muc4	APN	16	32,751,439 (GRCm38)	nonsense	probably null
IGL03335:Muc4	APN	16	32,753,021 (GRCm38)	missense	probably benign	0.01
IGL03411:Muc4	APN	16	32,754,318 (GRCm38)	missense	probably benign	0.01
multisplendored	UTSW	16	32,751,051 (GRCm38)	missense	possibly damaging	0.53
protean	UTSW	16	32,754,689 (GRCm38)	missense	probably benign	0.00
R0864_muc4_269	UTSW	16	32,752,002 (GRCm38)	missense	probably benign	0.01
3-1:Muc4	UTSW	16	32,770,394 (GRCm38)	missense	possibly damaging	0.86
3-1:Muc4	UTSW	16	32,760,251 (GRCm38)	splice site	probably benign
BB004:Muc4	UTSW	16	32,771,637 (GRCm38)	missense
BB014:Muc4	UTSW	16	32,771,637 (GRCm38)	missense
IGL02835:Muc4	UTSW	16	32,763,945 (GRCm38)	missense	probably benign	0.32
P0035:Muc4	UTSW	16	32,760,248 (GRCm38)	splice site	probably benign
PIT4131001:Muc4	UTSW	16	32,755,684 (GRCm38)	unclassified	probably benign
PIT4131001:Muc4	UTSW	16	32,755,699 (GRCm38)	unclassified	probably benign
PIT4131001:Muc4	UTSW	16	32,755,676 (GRCm38)	unclassified	probably benign
PIT4142001:Muc4	UTSW	16	32,755,684 (GRCm38)	unclassified	probably benign
PIT4142001:Muc4	UTSW	16	32,754,529 (GRCm38)	missense	probably benign	0.01
PIT4142001:Muc4	UTSW	16	32,755,676 (GRCm38)	unclassified	probably benign
PIT4366001:Muc4	UTSW	16	32,754,796 (GRCm38)	missense	unknown
PIT4531001:Muc4	UTSW	16	32,756,017 (GRCm38)	missense	unknown
R0119:Muc4	UTSW	16	32,750,195 (GRCm38)	critical splice acceptor site	probably benign
R0133:Muc4	UTSW	16	32,771,604 (GRCm38)	missense	possibly damaging	0.91
R0136:Muc4	UTSW	16	32,750,195 (GRCm38)	critical splice acceptor site	probably benign
R0243:Muc4	UTSW	16	32,765,746 (GRCm38)	missense	possibly damaging	0.53
R0277:Muc4	UTSW	16	32,755,690 (GRCm38)	unclassified	probably benign
R0299:Muc4	UTSW	16	32,750,195 (GRCm38)	critical splice acceptor site	probably benign
R0380:Muc4	UTSW	16	32,752,905 (GRCm38)	missense	probably benign	0.00
R0462:Muc4	UTSW	16	32,762,536 (GRCm38)	missense	possibly damaging	0.93
R0507:Muc4	UTSW	16	32,751,069 (GRCm38)	missense	probably benign	0.01
R0508:Muc4	UTSW	16	32,751,313 (GRCm38)	missense	possibly damaging	0.83
R0543:Muc4	UTSW	16	32,756,746 (GRCm38)	missense	unknown
R0578:Muc4	UTSW	16	32,755,690 (GRCm38)	unclassified	probably benign
R0617:Muc4	UTSW	16	32,752,107 (GRCm38)	missense	possibly damaging	0.83
R0656:Muc4	UTSW	16	32,751,670 (GRCm38)	missense	possibly damaging	0.91
R0726:Muc4	UTSW	16	32,769,827 (GRCm38)	missense	probably damaging	0.99
R0727:Muc4	UTSW	16	32,769,847 (GRCm38)	missense	probably benign	0.01
R0776:Muc4	UTSW	16	32,752,220 (GRCm38)	missense	probably benign	0.04
R0854:Muc4	UTSW	16	32,778,955 (GRCm38)	missense	possibly damaging	0.53
R0862:Muc4	UTSW	16	32,752,002 (GRCm38)	missense	probably benign	0.01
R0864:Muc4	UTSW	16	32,752,002 (GRCm38)	missense	probably benign	0.01
R0926:Muc4	UTSW	16	32,756,196 (GRCm38)	unclassified	probably benign
R0990:Muc4	UTSW	16	32,752,722 (GRCm38)	missense	probably benign	0.00
R1127:Muc4	UTSW	16	32,750,525 (GRCm38)	missense	possibly damaging	0.92
R1203:Muc4	UTSW	16	32,754,529 (GRCm38)	missense	probably benign	0.01
R1433:Muc4	UTSW	16	32,753,020 (GRCm38)	missense	probably benign	0.00
R1466:Muc4	UTSW	16	32,753,595 (GRCm38)	missense	probably benign	0.04
R1506:Muc4	UTSW	16	32,752,233 (GRCm38)	missense	possibly damaging	0.59
R1518:Muc4	UTSW	16	32,750,349 (GRCm38)	missense	possibly damaging	0.84
R1544:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R1584:Muc4	UTSW	16	32,753,595 (GRCm38)	missense	probably benign	0.04
R1593:Muc4	UTSW	16	32,754,686 (GRCm38)	missense	probably benign	0.00
R1601:Muc4	UTSW	16	32,755,501 (GRCm38)	unclassified	probably benign
R1611:Muc4	UTSW	16	32,750,986 (GRCm38)	missense	possibly damaging	0.86
R1673:Muc4	UTSW	16	32,756,902 (GRCm38)	missense	probably benign	0.11
R1717:Muc4	UTSW	16	32,753,405 (GRCm38)	missense	possibly damaging	0.53
R1822:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R1824:Muc4	UTSW	16	32,755,933 (GRCm38)	unclassified	probably benign
R1839:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R1846:Muc4	UTSW	16	32,752,369 (GRCm38)	missense	probably benign	0.01
R1864:Muc4	UTSW	16	32,756,251 (GRCm38)	unclassified	probably benign
R1868:Muc4	UTSW	16	32,756,341 (GRCm38)	missense	unknown
R1928:Muc4	UTSW	16	32,750,532 (GRCm38)	missense	probably damaging	0.99
R1942:Muc4	UTSW	16	32,750,642 (GRCm38)	missense	probably damaging	0.99
R2017:Muc4	UTSW	16	32,751,303 (GRCm38)	missense	possibly damaging	0.92
R2023:Muc4	UTSW	16	32,752,254 (GRCm38)	missense	probably benign	0.00
R2081:Muc4	UTSW	16	32,752,220 (GRCm38)	missense	probably benign	0.04
R2088:Muc4	UTSW	16	32,756,409 (GRCm38)	missense	unknown
R2121:Muc4	UTSW	16	32,760,238 (GRCm38)	missense	unknown
R2139:Muc4	UTSW	16	32,761,225 (GRCm38)	missense	unknown
R2158:Muc4	UTSW	16	32,754,563 (GRCm38)	missense	probably benign	0.10
R2165:Muc4	UTSW	16	32,750,476 (GRCm38)	missense	probably damaging	0.96
R2210:Muc4	UTSW	16	32,755,176 (GRCm38)	frame shift	probably null
R2225:Muc4	UTSW	16	32,766,942 (GRCm38)	missense	possibly damaging	0.73
R2225:Muc4	UTSW	16	32,755,891 (GRCm38)	unclassified	probably benign
R2269:Muc4	UTSW	16	32,754,529 (GRCm38)	missense	probably benign	0.01
R2679:Muc4	UTSW	16	32,757,472 (GRCm38)	missense	unknown
R3703:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R3816:Muc4	UTSW	16	32,754,529 (GRCm38)	missense	probably benign	0.01
R3909:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R4014:Muc4	UTSW	16	32,755,273 (GRCm38)	unclassified	probably benign
R4065:Muc4	UTSW	16	32,751,051 (GRCm38)	missense	possibly damaging	0.53
R4066:Muc4	UTSW	16	32,751,051 (GRCm38)	missense	possibly damaging	0.53
R4067:Muc4	UTSW	16	32,751,051 (GRCm38)	missense	possibly damaging	0.53
R4245:Muc4	UTSW	16	32,753,802 (GRCm38)	missense	probably benign	0.04
R4249:Muc4	UTSW	16	32,755,826 (GRCm38)	unclassified	probably benign
R4344:Muc4	UTSW	16	32,770,292 (GRCm38)	missense	possibly damaging	0.53
R4388:Muc4	UTSW	16	32,753,802 (GRCm38)	missense	probably benign	0.04
R4393:Muc4	UTSW	16	32,754,529 (GRCm38)	missense	probably benign	0.01
R4482:Muc4	UTSW	16	32,756,701 (GRCm38)	missense	unknown
R4523:Muc4	UTSW	16	32,736,336 (GRCm38)	utr 5 prime	probably benign
R4527:Muc4	UTSW	16	32,755,843 (GRCm38)	unclassified	probably benign
R4572:Muc4	UTSW	16	32,753,802 (GRCm38)	missense	probably benign	0.04
R4587:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R4614:Muc4	UTSW	16	32,757,058 (GRCm38)	missense	probably benign	0.03
R4635:Muc4	UTSW	16	32,753,802 (GRCm38)	missense	probably benign	0.04
R4661:Muc4	UTSW	16	32,769,277 (GRCm38)	missense	possibly damaging	0.71
R4701:Muc4	UTSW	16	32,755,846 (GRCm38)	unclassified	probably benign
R4730:Muc4	UTSW	16	32,751,214 (GRCm38)	missense	possibly damaging	0.86
R4740:Muc4	UTSW	16	32,775,903 (GRCm38)	missense	possibly damaging	0.91
R4762:Muc4	UTSW	16	32,753,625 (GRCm38)	unclassified	probably benign
R4818:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R4821:Muc4	UTSW	16	32,753,802 (GRCm38)	missense	probably benign	0.04
R4825:Muc4	UTSW	16	32,751,747 (GRCm38)	missense	probably benign	0.41
R4830:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R4860:Muc4	UTSW	16	32,754,625 (GRCm38)	missense	probably benign	0.18
R4860:Muc4	UTSW	16	32,754,616 (GRCm38)	missense	probably benign	0.35
R4869:Muc4	UTSW	16	32,754,836 (GRCm38)	unclassified	probably benign
R4934:Muc4	UTSW	16	32,756,098 (GRCm38)	unclassified	probably benign
R4959:Muc4	UTSW	16	32,754,319 (GRCm38)	missense	possibly damaging	0.73
R4969:Muc4	UTSW	16	32,754,572 (GRCm38)	missense	probably benign	0.01
R4995:Muc4	UTSW	16	32,754,041 (GRCm38)	missense	probably benign	0.01
R4995:Muc4	UTSW	16	32,754,214 (GRCm38)	missense	probably benign	0.01
R4999:Muc4	UTSW	16	32,756,296 (GRCm38)	unclassified	probably benign
R5073:Muc4	UTSW	16	32,754,529 (GRCm38)	missense	probably benign	0.01
R5075:Muc4	UTSW	16	32,754,794 (GRCm38)	unclassified	probably benign
R5152:Muc4	UTSW	16	32,757,058 (GRCm38)	nonsense	probably null
R5161:Muc4	UTSW	16	32,762,521 (GRCm38)	missense	probably damaging	0.98
R5174:Muc4	UTSW	16	32,751,738 (GRCm38)	missense	possibly damaging	0.84
R5268:Muc4	UTSW	16	32,751,666 (GRCm38)	missense	possibly damaging	0.83
R5447:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R5474:Muc4	UTSW	16	32,761,261 (GRCm38)	missense	unknown
R5567:Muc4	UTSW	16	32,777,692 (GRCm38)	missense	possibly damaging	0.72
R5570:Muc4	UTSW	16	32,777,692 (GRCm38)	missense	possibly damaging	0.72
R5618:Muc4	UTSW	16	32,754,253 (GRCm38)	missense	probably benign	0.01
R5665:Muc4	UTSW	16	32,750,782 (GRCm38)	missense	probably benign	0.33
R5667:Muc4	UTSW	16	32,753,720 (GRCm38)	missense	probably benign	0.01
R5671:Muc4	UTSW	16	32,753,720 (GRCm38)	missense	probably benign	0.01
R5693:Muc4	UTSW	16	32,776,807 (GRCm38)	missense	possibly damaging	0.53
R5703:Muc4	UTSW	16	32,736,241 (GRCm38)	nonsense	probably null
R5708:Muc4	UTSW	16	32,754,769 (GRCm38)	unclassified	probably benign
R5715:Muc4	UTSW	16	32,751,916 (GRCm38)	missense	possibly damaging	0.92
R5849:Muc4	UTSW	16	32,774,839 (GRCm38)	missense	possibly damaging	0.53
R5873:Muc4	UTSW	16	32,751,295 (GRCm38)	missense	possibly damaging	0.61
R5930:Muc4	UTSW	16	32,751,705 (GRCm38)	missense	probably benign	0.41
R5933:Muc4	UTSW	16	32,753,052 (GRCm38)	missense	probably benign	0.01
R5966:Muc4	UTSW	16	32,756,278 (GRCm38)	unclassified	probably benign
R6062:Muc4	UTSW	16	32,759,308 (GRCm38)	missense	unknown
R6067:Muc4	UTSW	16	32,754,529 (GRCm38)	missense	probably benign	0.01
R6067:Muc4	UTSW	16	32,755,247 (GRCm38)	unclassified	probably benign
R6078:Muc4	UTSW	16	32,755,247 (GRCm38)	unclassified	probably benign
R6079:Muc4	UTSW	16	32,755,247 (GRCm38)	unclassified	probably benign
R6112:Muc4	UTSW	16	32,775,783 (GRCm38)	missense	possibly damaging	0.86
R6120:Muc4	UTSW	16	32,756,795 (GRCm38)	missense	unknown
R6144:Muc4	UTSW	16	32,766,924 (GRCm38)	missense	possibly damaging	0.53
R6148:Muc4	UTSW	16	32,753,802 (GRCm38)	missense	probably benign	0.04
R6173:Muc4	UTSW	16	32,736,140 (GRCm38)	start gained	probably benign
R6268:Muc4	UTSW	16	32,768,767 (GRCm38)	missense	probably damaging	0.99
R6299:Muc4	UTSW	16	32,752,035 (GRCm38)	missense	possibly damaging	0.48
R6307:Muc4	UTSW	16	32,753,946 (GRCm38)	missense	possibly damaging	0.56
R6354:Muc4	UTSW	16	32,754,358 (GRCm38)	missense	probably benign	0.19
R6361:Muc4	UTSW	16	32,767,351 (GRCm38)	missense	probably benign	0.32
R6375:Muc4	UTSW	16	32,736,243 (GRCm38)	utr 5 prime	probably benign
R6378:Muc4	UTSW	16	32,778,946 (GRCm38)	missense	probably benign	0.33
R6418:Muc4	UTSW	16	32,751,789 (GRCm38)	missense	possibly damaging	0.68
R6458:Muc4	UTSW	16	32,759,320 (GRCm38)	critical splice donor site	probably null
R6527:Muc4	UTSW	16	32,753,433 (GRCm38)	missense	probably benign	0.01
R6616:Muc4	UTSW	16	32,782,008 (GRCm38)	missense	possibly damaging	0.93
R6636:Muc4	UTSW	16	32,753,964 (GRCm38)	missense	probably benign	0.01
R6637:Muc4	UTSW	16	32,753,964 (GRCm38)	missense	probably benign	0.01
R6915:Muc4	UTSW	16	32,766,938 (GRCm38)	missense	probably benign	0.18
R6947:Muc4	UTSW	16	32,775,803 (GRCm38)	missense	possibly damaging	0.91
R6976:Muc4	UTSW	16	32,762,518 (GRCm38)	missense	possibly damaging	0.92
R6985:Muc4	UTSW	16	32,751,999 (GRCm38)	missense	probably benign	0.00
R7020:Muc4	UTSW	16	32,751,810 (GRCm38)	nonsense	probably null
R7033:Muc4	UTSW	16	32,756,324 (GRCm38)	unclassified	probably benign
R7098:Muc4	UTSW	16	32,757,091 (GRCm38)	missense
R7123:Muc4	UTSW	16	32,750,691 (GRCm38)	missense	possibly damaging	0.83
R7173:Muc4	UTSW	16	32,762,488 (GRCm38)	missense	probably damaging	0.97
R7178:Muc4	UTSW	16	32,752,788 (GRCm38)	missense	unknown
R7294:Muc4	UTSW	16	32,756,461 (GRCm38)	missense	possibly damaging	0.53
R7318:Muc4	UTSW	16	32,755,336 (GRCm38)	missense	unknown
R7361:Muc4	UTSW	16	32,754,670 (GRCm38)	missense	probably benign	0.18
R7380:Muc4	UTSW	16	32,755,366 (GRCm38)	missense	unknown
R7381:Muc4	UTSW	16	32,780,915 (GRCm38)	missense
R7411:Muc4	UTSW	16	32,751,322 (GRCm38)	missense	probably benign	0.12
R7422:Muc4	UTSW	16	32,754,689 (GRCm38)	missense	probably benign	0.00
R7482:Muc4	UTSW	16	32,766,950 (GRCm38)	missense
R7539:Muc4	UTSW	16	32,756,396 (GRCm38)	missense
R7544:Muc4	UTSW	16	32,736,198 (GRCm38)	start codon destroyed	probably null
R7574:Muc4	UTSW	16	32,753,411 (GRCm38)	missense	probably benign	0.18
R7576:Muc4	UTSW	16	32,754,500 (GRCm38)	missense	probably benign	0.10
R7585:Muc4	UTSW	16	32,765,702 (GRCm38)	missense
R7596:Muc4	UTSW	16	32,753,930 (GRCm38)	missense	probably benign	0.01
R7597:Muc4	UTSW	16	32,753,930 (GRCm38)	missense	probably benign	0.01
R7602:Muc4	UTSW	16	32,753,930 (GRCm38)	missense	probably benign	0.01
R7616:Muc4	UTSW	16	32,752,361 (GRCm38)	nonsense	probably null
R7639:Muc4	UTSW	16	32,753,930 (GRCm38)	missense	probably benign	0.01
R7640:Muc4	UTSW	16	32,760,105 (GRCm38)	missense
R7651:Muc4	UTSW	16	32,756,575 (GRCm38)	missense
R7688:Muc4	UTSW	16	32,751,460 (GRCm38)	missense	possibly damaging	0.68
R7689:Muc4	UTSW	16	32,753,011 (GRCm38)	missense	probably benign	0.10
R7752:Muc4	UTSW	16	32,768,734 (GRCm38)	missense
R7763:Muc4	UTSW	16	32,753,311 (GRCm38)	missense	probably benign	0.10
R7768:Muc4	UTSW	16	32,756,194 (GRCm38)	missense	unknown
R7787:Muc4	UTSW	16	32,753,930 (GRCm38)	missense	probably benign	0.01
R7789:Muc4	UTSW	16	32,753,930 (GRCm38)	missense	probably benign	0.01
R7838:Muc4	UTSW	16	32,752,558 (GRCm38)	nonsense	probably null
R7871:Muc4	UTSW	16	32,754,935 (GRCm38)	missense	unknown
R7921:Muc4	UTSW	16	32,751,321 (GRCm38)	missense	possibly damaging	0.68
R7927:Muc4	UTSW	16	32,771,637 (GRCm38)	missense
R7930:Muc4	UTSW	16	32,754,078 (GRCm38)	unclassified	probably benign
R8000:Muc4	UTSW	16	32,753,930 (GRCm38)	missense	probably benign	0.01
R8062:Muc4	UTSW	16	32,756,749 (GRCm38)	missense
R8096:Muc4	UTSW	16	32,755,390 (GRCm38)	missense	unknown
R8115:Muc4	UTSW	16	32,755,304 (GRCm38)	missense	unknown
R8162:Muc4	UTSW	16	32,750,379 (GRCm38)	missense	unknown
R8220:Muc4	UTSW	16	32,755,327 (GRCm38)	missense	unknown
R8260:Muc4	UTSW	16	32,754,076 (GRCm38)	unclassified	probably benign
R8290:Muc4	UTSW	16	32,754,316 (GRCm38)	missense	probably benign	0.01
R8299:Muc4	UTSW	16	32,755,897 (GRCm38)	missense	unknown
R8313:Muc4	UTSW	16	32,753,423 (GRCm38)	missense	probably benign	0.04
R8356:Muc4	UTSW	16	32,754,076 (GRCm38)	unclassified	probably benign
R8463:Muc4	UTSW	16	32,752,401 (GRCm38)	missense	probably benign	0.00
R8479:Muc4	UTSW	16	32,753,508 (GRCm38)	missense	possibly damaging	0.59
R8480:Muc4	UTSW	16	32,752,993 (GRCm38)	missense	probably benign	0.20
R8510:Muc4	UTSW	16	32,754,076 (GRCm38)	unclassified	probably benign
R8515:Muc4	UTSW	16	32,755,255 (GRCm38)	missense	unknown
R8559:Muc4	UTSW	16	32,754,715 (GRCm38)	missense	unknown
R8685:Muc4	UTSW	16	32,753,930 (GRCm38)	missense	probably benign	0.01
R8687:Muc4	UTSW	16	32,753,930 (GRCm38)	missense	probably benign	0.01
R8728:Muc4	UTSW	16	32,754,952 (GRCm38)	missense	unknown
R8754:Muc4	UTSW	16	32,781,986 (GRCm38)	missense
R8845:Muc4	UTSW	16	32,756,515 (GRCm38)	missense	possibly damaging	0.86
R8852:Muc4	UTSW	16	32,750,643 (GRCm38)	missense	possibly damaging	0.66
R8863:Muc4	UTSW	16	32,751,462 (GRCm38)	missense	possibly damaging	0.84
R8896:Muc4	UTSW	16	32,754,673 (GRCm38)	missense	probably benign	0.01
R8929:Muc4	UTSW	16	32,754,017 (GRCm38)	missense	possibly damaging	0.53
R8929:Muc4	UTSW	16	32,753,994 (GRCm38)	missense	probably benign	0.00
R8990:Muc4	UTSW	16	32,752,209 (GRCm38)	missense	probably benign	0.01
R9018:Muc4	UTSW	16	32,762,536 (GRCm38)	missense
R9034:Muc4	UTSW	16	32,754,076 (GRCm38)	unclassified	probably benign
R9064:Muc4	UTSW	16	32,754,397 (GRCm38)	missense	possibly damaging	0.53
R9068:Muc4	UTSW	16	32,754,076 (GRCm38)	unclassified	probably benign
R9086:Muc4	UTSW	16	32,757,468 (GRCm38)	missense
R9151:Muc4	UTSW	16	32,752,617 (GRCm38)	missense	probably benign	0.01
R9187:Muc4	UTSW	16	32,768,728 (GRCm38)	missense
R9336:Muc4	UTSW	16	32,750,938 (GRCm38)	missense	possibly damaging	0.86
R9343:Muc4	UTSW	16	32,751,153 (GRCm38)	missense	possibly damaging	0.53
R9363:Muc4	UTSW	16	32,756,618 (GRCm38)	missense
R9429:Muc4	UTSW	16	32,755,724 (GRCm38)	missense	unknown
R9570:Muc4	UTSW	16	32,750,877 (GRCm38)	missense	possibly damaging	0.53
R9609:Muc4	UTSW	16	32,756,860 (GRCm38)	missense
R9696:Muc4	UTSW	16	32,753,284 (GRCm38)	missense	probably benign	0.00
R9709:Muc4	UTSW	16	32,770,270 (GRCm38)	missense
R9743:Muc4	UTSW	16	32,780,824 (GRCm38)	missense
R9747:Muc4	UTSW	16	32,754,698 (GRCm38)	missense	probably benign	0.01
RF014:Muc4	UTSW	16	32,751,858 (GRCm38)	missense	probably damaging	0.98
U15987:Muc4	UTSW	16	32,754,529 (GRCm38)	missense	probably benign	0.01
U15987:Muc4	UTSW	16	32,755,247 (GRCm38)	unclassified	probably benign
V5622:Muc4	UTSW	16	32,751,825 (GRCm38)	missense	probably benign	0.00
X0018:Muc4	UTSW	16	32,755,481 (GRCm38)	unclassified	probably benign
X0028:Muc4	UTSW	16	32,759,319 (GRCm38)	critical splice donor site	probably null
Z1088:Muc4	UTSW	16	32,756,076 (GRCm38)	unclassified	probably benign
Z1176:Muc4	UTSW	16	32,768,730 (GRCm38)	missense	possibly damaging	0.65
Z1177:Muc4	UTSW	16	32,767,393 (GRCm38)	nonsense	probably null
Z1177:Muc4	UTSW	16	32,774,862 (GRCm38)	missense
Z1177:Muc4	UTSW	16	32,767,394 (GRCm38)	missense

Predicted Primers

PCR Primer
(F):5'- GCAGCTCAAGTTCTACACCTGCC -3'
(R):5'- TCATGTTCTGGGAGGAAGGTGAGAC -3'

Sequencing Primer
(F):5'- AGAACATGTCAACAGTGTCAAC -3'
(R):5'- GTCTCACCAGGAGAGATTGTTC -3'

Posted On

2014-03-28