Mutagenetix > Incidental Mutation

Incidental Mutation 'R0013:Mroh4'

166164

Institutional Source

Beutler Lab

Gene Symbol

Mroh4

Ensembl Gene

ENSMUSG00000022603

Gene Name

maestro heat-like repeat family member 4

Synonyms

1700016M24Rik

MMRRC Submission

038308-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R0013 (G1)

Quality Score

Status

Validated

Chromosome

Chromosomal Location

74606029-74636353 bp(-) (GRCm38)

Type of Mutation

splice site

DNA Base Change (assembly)

T to A at 74608237 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000117011 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000023271] [ENSMUST00000137963]

AlphaFold

G3X8W1

Predicted Effect

probably benign
Transcript: ENSMUST00000023271

SMART Domains

Protein: ENSMUSP00000023271
Gene: ENSMUSG00000022603

Domain	Start	End	E-Value	Type
low complexity region	1	12	N/A	INTRINSIC
low complexity region	326	337	N/A	INTRINSIC
low complexity region	428	435	N/A	INTRINSIC
low complexity region	520	534	N/A	INTRINSIC
low complexity region	572	591	N/A	INTRINSIC
SCOP:d1ee4a_	709	852	3e-5	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000137963

SMART Domains

Protein: ENSMUSP00000117011
Gene: ENSMUSG00000022603

Domain	Start	End	E-Value	Type
low complexity region	257	268	N/A	INTRINSIC
low complexity region	359	366	N/A	INTRINSIC
low complexity region	451	465	N/A	INTRINSIC
low complexity region	503	522	N/A	INTRINSIC
SCOP:d1ee4a_	640	783	3e-5	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000176592

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000177179

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 96.9%
20x: 94.8%

Validation Efficiency

94% (79/84)

Allele List at MGI

Other mutations in this stock

Total: 79 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2610028H24Rik	A	G	10: 76,457,512	M156V	probably benign	Het
Adnp2	A	T	18: 80,129,745	V483D	probably damaging	Het
Aff1	G	T	5: 103,828,484	E491*	probably null	Het
Agl	A	T	3: 116,776,608	C911*	probably null	Het
Akt2	A	G	7: 27,636,058	D284G	probably damaging	Het
Alox15	A	G	11: 70,349,635	M240T	possibly damaging	Het
Antxr2	A	G	5: 97,979,985	V229A	probably damaging	Het
Arap2	G	A	5: 62,683,484	L680F	probably damaging	Het
Btaf1	A	G	19: 36,958,373	T188A	probably benign	Het
Btnl6	G	A	17: 34,515,531	Q86*	probably null	Het
C2cd3	T	A	7: 100,416,062	L685H	probably damaging	Het
Cdh23	T	C	10: 60,413,173	T878A	possibly damaging	Het
Clec4b2	T	C	6: 123,202,149	Y137H	probably damaging	Het
Dchs1	T	A	7: 105,755,836	T2500S	possibly damaging	Het
Def6	A	G	17: 28,217,092	Y75C	probably damaging	Het
Dhx33	A	T	11: 70,993,635	F448L	probably damaging	Het
Dner	C	T	1: 84,494,893		probably benign	Het
Dnmbp	G	A	19: 43,902,231	P366S	probably benign	Het
Eif4g3	T	C	4: 138,175,848	C1160R	possibly damaging	Het
Elmod1	G	A	9: 53,912,901		probably benign	Het
Faah	C	A	4: 116,004,391	L305F	probably damaging	Het
Fam71b	A	G	11: 46,406,804	T312A	unknown	Het
Flt1	A	G	5: 147,571,014		probably benign	Het
Fyco1	A	T	9: 123,822,406	N1196K	probably benign	Het
Galnt18	T	C	7: 111,554,457	N320S	probably damaging	Het
Glp2r	C	A	11: 67,709,712	G437V	possibly damaging	Het
Gm4884	T	C	7: 41,044,292	S562P	probably damaging	Het
Gm9936	A	G	5: 114,857,347		probably benign	Het
Gpn2	C	A	4: 133,584,792	P112T	probably damaging	Het
Grm4	A	G	17: 27,431,575	Y816H	probably benign	Het
Helz2	A	T	2: 181,240,959	S14T	probably benign	Het
Htt	T	C	5: 34,820,104	L778P	probably benign	Het
Il11ra1	T	C	4: 41,765,060	S129P	probably damaging	Het
Ints11	T	C	4: 155,887,168	F315S	probably damaging	Het
Itga11	A	T	9: 62,776,613	N1059Y	possibly damaging	Het
Jak3	A	G	8: 71,684,327	S716G	probably damaging	Het
Kcns1	G	T	2: 164,168,643	D65E	probably benign	Het
Kdm5d	A	T	Y: 941,715	K1305N	probably benign	Het
Kif26a	G	T	12: 112,177,880	V1523L	probably benign	Het
Mboat7	A	G	7: 3,683,822	S340P	probably damaging	Het
Mctp2	T	C	7: 72,229,408	I234V	probably benign	Het
Mex3c	G	A	18: 73,590,551	A572T	probably benign	Het
Mpp3	C	A	11: 102,005,425	R424L	probably benign	Het
Myo9a	A	T	9: 59,860,206		probably benign	Het
Myog	T	A	1: 134,290,235	H60Q	probably damaging	Het
Nlrp9a	T	A	7: 26,571,225		probably null	Het
Notch1	A	G	2: 26,473,818	V868A	possibly damaging	Het
Olfr352	A	G	2: 36,870,160	N198S	probably damaging	Het
Olfr59	T	A	11: 74,289,051	I135N	possibly damaging	Het
Olfr73	T	C	2: 88,034,266	Y291C	possibly damaging	Het
Olfr980	A	T	9: 40,006,355	I198N	probably damaging	Het
Pink1	T	C	4: 138,317,401	T342A	probably benign	Het
Plb1	T	A	5: 32,349,615		probably benign	Het
Plec	T	C	15: 76,178,246	D2524G	probably damaging	Het
Plekhg4	G	T	8: 105,375,396	E6*	probably null	Het
Polq	T	C	16: 37,061,839	F1455S	possibly damaging	Het
Ppm1e	A	G	11: 87,249,058		probably benign	Het
Prkaca	G	A	8: 83,988,303	M119I	possibly damaging	Het
Prss46	G	T	9: 110,850,055	S108I	probably damaging	Het
Ptma	C	T	1: 86,529,776		probably benign	Het
Rab11fip4	C	T	11: 79,689,653	T437M	probably benign	Het
Rngtt	T	A	4: 33,379,409	M437K	probably benign	Het
Rrn3	T	A	16: 13,813,113	D604E	possibly damaging	Het
Scn4a	A	G	11: 106,348,405		probably benign	Het
Sis	A	G	3: 72,910,476	L1468P	possibly damaging	Het
Slit3	A	G	11: 35,707,918	M1450V	probably benign	Het
Smg5	T	C	3: 88,349,233	S269P	probably benign	Het
Sntg1	T	C	1: 8,463,462	T323A	probably damaging	Het
Son	C	T	16: 91,651,662	T37I	probably damaging	Het
Stk17b	T	C	1: 53,764,132	I41M	probably benign	Het
Tgm5	T	A	2: 121,076,882	Y120F	probably damaging	Het
Tppp	A	G	13: 74,021,360	K73R	possibly damaging	Het
Ttn	C	A	2: 76,739,158	K27130N	probably damaging	Het
Ttn	C	T	2: 76,907,752	V4148I	probably benign	Het
Uba7	A	T	9: 107,978,249	Y375F	probably damaging	Het
Ugcg	T	C	4: 59,213,931	L171P	possibly damaging	Het
Vsig2	T	C	9: 37,542,576		probably benign	Het
Zcchc11	T	A	4: 108,530,955		probably benign	Het
Zfp839	T	A	12: 110,868,386	S692T	possibly damaging	Het

Other mutations in Mroh4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01645:Mroh4	APN	15	74611358	splice site	probably benign
IGL02370:Mroh4	APN	15	74625541	missense	probably benign	0.00
IGL02598:Mroh4	APN	15	74611243	critical splice donor site	probably null
IGL02644:Mroh4	APN	15	74610375	missense	possibly damaging	0.90
IGL02666:Mroh4	APN	15	74609775	missense	probably benign	0.04
IGL02723:Mroh4	APN	15	74608237	splice site	probably benign
IGL02724:Mroh4	APN	15	74606151	missense	probably benign	0.00
IGL03000:Mroh4	APN	15	74616114	missense	probably benign
IGL03103:Mroh4	APN	15	74616159	missense	possibly damaging	0.47
IGL03194:Mroh4	APN	15	74611539	missense	probably damaging	1.00
R0042:Mroh4	UTSW	15	74610305	missense	probably damaging	0.99
R0042:Mroh4	UTSW	15	74610305	missense	probably damaging	0.99
R0294:Mroh4	UTSW	15	74606149	missense	probably benign
R0346:Mroh4	UTSW	15	74614292	splice site	probably benign
R0545:Mroh4	UTSW	15	74625427	missense	probably benign	0.00
R0688:Mroh4	UTSW	15	74606678	missense	probably damaging	0.98
R1838:Mroh4	UTSW	15	74616113	missense	probably benign	0.03
R2037:Mroh4	UTSW	15	74609761	missense	possibly damaging	0.91
R4725:Mroh4	UTSW	15	74616107	missense	probably damaging	0.99
R4786:Mroh4	UTSW	15	74610234	missense	probably benign	0.08
R4798:Mroh4	UTSW	15	74626179	missense	probably damaging	1.00
R4945:Mroh4	UTSW	15	74612008	missense	probably benign	0.00
R5065:Mroh4	UTSW	15	74628270	splice site	probably null
R5476:Mroh4	UTSW	15	74611661	missense	probably benign	0.15
R5509:Mroh4	UTSW	15	74606154	missense	probably benign	0.00
R5527:Mroh4	UTSW	15	74615016	missense	probably damaging	1.00
R5662:Mroh4	UTSW	15	74625428	missense	possibly damaging	0.63
R5818:Mroh4	UTSW	15	74611982	missense	probably damaging	0.98
R5861:Mroh4	UTSW	15	74606607	intron	probably benign
R5886:Mroh4	UTSW	15	74606447	missense	possibly damaging	0.90
R5935:Mroh4	UTSW	15	74621154	missense	probably damaging	1.00
R6008:Mroh4	UTSW	15	74625472	nonsense	probably null
R6658:Mroh4	UTSW	15	74621129	missense	possibly damaging	0.83
R6689:Mroh4	UTSW	15	74612003	missense	probably damaging	1.00
R6739:Mroh4	UTSW	15	74609719	missense	probably benign	0.10
R6888:Mroh4	UTSW	15	74613249	missense	possibly damaging	0.93
R7088:Mroh4	UTSW	15	74626144	missense	probably benign	0.25
R7260:Mroh4	UTSW	15	74608129	missense	possibly damaging	0.83
R7365:Mroh4	UTSW	15	74610371	nonsense	probably null
R7735:Mroh4	UTSW	15	74625508	missense	probably damaging	0.98
R7763:Mroh4	UTSW	15	74624705	missense	probably damaging	0.99
R7945:Mroh4	UTSW	15	74624705	missense	probably damaging	0.99
R8090:Mroh4	UTSW	15	74624701	missense	probably benign	0.41
R8242:Mroh4	UTSW	15	74616308	missense	possibly damaging	0.47
R8978:Mroh4	UTSW	15	74627624	missense	probably benign	0.00
R9004:Mroh4	UTSW	15	74614322	missense	possibly damaging	0.65
R9083:Mroh4	UTSW	15	74626291	missense	probably damaging	1.00
R9172:Mroh4	UTSW	15	74606112	makesense	probably null
R9248:Mroh4	UTSW	15	74613318	missense	possibly damaging	0.59
R9320:Mroh4	UTSW	15	74611556	missense	probably damaging	1.00
R9356:Mroh4	UTSW	15	74610911	missense	probably benign	0.05
R9512:Mroh4	UTSW	15	74613246	missense	probably benign	0.18
Z1177:Mroh4	UTSW	15	74627720	missense	probably damaging	1.00
Z1177:Mroh4	UTSW	15	74628002	missense	possibly damaging	0.83

Predicted Primers

PCR Primer
(F):5'- TCAGAAAGCTCGGAGGTGACTGTG -3'
(R):5'- TCTCCCAGGCTTCCAAGAGTAAGAC -3'

Sequencing Primer
(F):5'- TGTGCAGCCACCACACC -3'
(R):5'- ttaacaatccaaatgtagcccag -3'

Posted On

2014-04-10