Mutagenetix > Incidental Mutation

Incidental Mutation 'R1531:Pclo'

166186

Institutional Source

Beutler Lab

Gene Symbol

Pclo

Ensembl Gene

ENSMUSG00000061601

Gene Name

piccolo (presynaptic cytomatrix protein)

Synonyms

Acz, Pico

MMRRC Submission

039570-MU

Accession Numbers

Ncbi RefSeq: NM_011995.4; NM_001110796.1; MGI:1349390

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R1531 (G1)

Quality Score

201

Status

Not validated

Chromosome

Chromosomal Location

14514918-14863459 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

C to G at 14521903 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Proline to Arginine at position 434 (P434R)

Ref Sequence

ENSEMBL: ENSMUSP00000138419 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000030691] [ENSMUST00000182407]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000030691
AA Change: P434R

PolyPhen 2 Score 0.993 (Sensitivity: 0.70; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000030691
Gene: ENSMUSG00000061601
AA Change: P434R

Domain	Start	End	E-Value	Type
low complexity region	4	30	N/A	INTRINSIC
low complexity region	90	106	N/A	INTRINSIC
internal_repeat_2	192	304	1.23e-13	PROSPERO
internal_repeat_2	305	389	1.23e-13	PROSPERO
low complexity region	393	489	N/A	INTRINSIC
low complexity region	513	525	N/A	INTRINSIC
Pfam:zf-piccolo	530	589	5.2e-30	PFAM
low complexity region	636	647	N/A	INTRINSIC
low complexity region	657	686	N/A	INTRINSIC
low complexity region	709	720	N/A	INTRINSIC
low complexity region	722	738	N/A	INTRINSIC
low complexity region	798	824	N/A	INTRINSIC
low complexity region	825	839	N/A	INTRINSIC
low complexity region	898	910	N/A	INTRINSIC
low complexity region	961	978	N/A	INTRINSIC
Pfam:zf-piccolo	995	1053	6.7e-33	PFAM
low complexity region	1070	1090	N/A	INTRINSIC
low complexity region	1092	1107	N/A	INTRINSIC
low complexity region	1150	1179	N/A	INTRINSIC
internal_repeat_4	1196	1249	1.65e-5	PROSPERO
low complexity region	1275	1289	N/A	INTRINSIC
low complexity region	1320	1331	N/A	INTRINSIC
low complexity region	1332	1343	N/A	INTRINSIC
low complexity region	1397	1411	N/A	INTRINSIC
low complexity region	1444	1457	N/A	INTRINSIC
low complexity region	1497	1511	N/A	INTRINSIC
low complexity region	1668	1681	N/A	INTRINSIC
coiled coil region	1704	1748	N/A	INTRINSIC
low complexity region	1789	1800	N/A	INTRINSIC
low complexity region	2046	2061	N/A	INTRINSIC
low complexity region	2109	2137	N/A	INTRINSIC
low complexity region	2335	2366	N/A	INTRINSIC
low complexity region	2374	2389	N/A	INTRINSIC
low complexity region	2413	2423	N/A	INTRINSIC
low complexity region	2487	2498	N/A	INTRINSIC
low complexity region	2605	2633	N/A	INTRINSIC
low complexity region	2727	2738	N/A	INTRINSIC
low complexity region	2792	2808	N/A	INTRINSIC
low complexity region	3117	3128	N/A	INTRINSIC
coiled coil region	3136	3189	N/A	INTRINSIC
coiled coil region	3210	3243	N/A	INTRINSIC
internal_repeat_4	3473	3521	1.65e-5	PROSPERO
coiled coil region	3688	3731	N/A	INTRINSIC
low complexity region	3735	3750	N/A	INTRINSIC
low complexity region	3863	3883	N/A	INTRINSIC
low complexity region	3947	3961	N/A	INTRINSIC
low complexity region	4004	4015	N/A	INTRINSIC
low complexity region	4149	4160	N/A	INTRINSIC
low complexity region	4185	4196	N/A	INTRINSIC
low complexity region	4210	4225	N/A	INTRINSIC
low complexity region	4229	4245	N/A	INTRINSIC
PDZ	4439	4518	3.74e-14	SMART
low complexity region	4575	4602	N/A	INTRINSIC
C2	4636	4750	1.44e-21	SMART
low complexity region	4764	4778	N/A	INTRINSIC
low complexity region	4801	4817	N/A	INTRINSIC
low complexity region	4840	4850	N/A	INTRINSIC
low complexity region	4870	4892	N/A	INTRINSIC
C2	4951	5057	8.56e-15	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000182407
AA Change: P434R

PolyPhen 2 Score 0.993 (Sensitivity: 0.70; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000138419
Gene: ENSMUSG00000061601
AA Change: P434R

Domain	Start	End	E-Value	Type
low complexity region	4	30	N/A	INTRINSIC
low complexity region	90	106	N/A	INTRINSIC
internal_repeat_2	192	304	1.06e-13	PROSPERO
internal_repeat_2	305	389	1.06e-13	PROSPERO
low complexity region	393	489	N/A	INTRINSIC
low complexity region	513	525	N/A	INTRINSIC
Pfam:zf-piccolo	528	589	3e-36	PFAM
low complexity region	636	647	N/A	INTRINSIC
low complexity region	657	686	N/A	INTRINSIC
low complexity region	709	720	N/A	INTRINSIC
low complexity region	722	738	N/A	INTRINSIC
low complexity region	798	824	N/A	INTRINSIC
low complexity region	825	839	N/A	INTRINSIC
low complexity region	898	910	N/A	INTRINSIC
low complexity region	961	978	N/A	INTRINSIC
Pfam:zf-piccolo	993	1053	4.3e-37	PFAM
low complexity region	1070	1090	N/A	INTRINSIC
low complexity region	1092	1107	N/A	INTRINSIC
low complexity region	1150	1179	N/A	INTRINSIC
internal_repeat_3	1196	1249	1.46e-5	PROSPERO
low complexity region	1275	1289	N/A	INTRINSIC
low complexity region	1320	1331	N/A	INTRINSIC
low complexity region	1332	1343	N/A	INTRINSIC
low complexity region	1397	1411	N/A	INTRINSIC
low complexity region	1444	1457	N/A	INTRINSIC
low complexity region	1497	1511	N/A	INTRINSIC
low complexity region	1668	1681	N/A	INTRINSIC
coiled coil region	1704	1748	N/A	INTRINSIC
low complexity region	1789	1800	N/A	INTRINSIC
low complexity region	2046	2061	N/A	INTRINSIC
low complexity region	2109	2137	N/A	INTRINSIC
low complexity region	2335	2366	N/A	INTRINSIC
low complexity region	2374	2389	N/A	INTRINSIC
low complexity region	2413	2423	N/A	INTRINSIC
low complexity region	2487	2498	N/A	INTRINSIC
low complexity region	2605	2633	N/A	INTRINSIC
low complexity region	2727	2738	N/A	INTRINSIC
low complexity region	2792	2808	N/A	INTRINSIC
low complexity region	3117	3128	N/A	INTRINSIC
coiled coil region	3136	3189	N/A	INTRINSIC
coiled coil region	3210	3243	N/A	INTRINSIC
internal_repeat_3	3473	3521	1.46e-5	PROSPERO
coiled coil region	3688	3731	N/A	INTRINSIC
low complexity region	3735	3750	N/A	INTRINSIC
low complexity region	3863	3883	N/A	INTRINSIC
low complexity region	3947	3961	N/A	INTRINSIC
low complexity region	4004	4015	N/A	INTRINSIC
low complexity region	4149	4160	N/A	INTRINSIC
low complexity region	4185	4196	N/A	INTRINSIC
low complexity region	4210	4225	N/A	INTRINSIC
low complexity region	4229	4245	N/A	INTRINSIC
PDZ	4439	4518	3.74e-14	SMART
low complexity region	4575	4602	N/A	INTRINSIC
C2	4636	4750	1.44e-21	SMART
low complexity region	4764	4778	N/A	INTRINSIC
low complexity region	4801	4817	N/A	INTRINSIC
low complexity region	4840	4850	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000182426

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000182496

Coding Region Coverage

1x: 99.0%
3x: 98.1%
10x: 95.6%
20x: 90.3%

Validation Efficiency

MGI Phenotype

Strain: 3699630; 4442915; 4442914
Lethality: D60
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is part of the presynaptic cytoskeletal matrix, which is involved in establishing active synaptic zones and in synaptic vesicle trafficking. Variations in this gene have been associated with bipolar disorder and major depressive disorder. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]
PHENOTYPE: Mice homozygous for one deletion of Pclo are viable and fertile, and display no overt abnormal phenotype. Mice homozygous for another knock-out allele exhibit some premature lethality, decreased body size, and abnormal synaptic vesicle number. [provided by MGI curators]

Allele List at MGI

All alleles(7) : Targeted(5) Gene trapped(2)

Other mutations in this stock

Total: 85 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700021F05Rik	A	G	10: 43,525,320 (GRCm38)	V211A	probably benign	Het
Agtpbp1	T	A	13: 59,500,634 (GRCm38)		probably null	Het
Aldh3b2	T	G	19: 3,977,543 (GRCm38)	F28C	probably damaging	Het
Apaf1	T	C	10: 91,054,521 (GRCm38)	N540S	probably damaging	Het
Apob	T	C	12: 7,997,880 (GRCm38)	I953T	possibly damaging	Het
Arhgef1	A	G	7: 24,924,998 (GRCm38)	T816A	probably damaging	Het
Arid4a	T	A	12: 71,076,005 (GRCm38)	F1053L	probably benign	Het
Atxn1l	A	C	8: 109,732,059 (GRCm38)	F524V	probably damaging	Het
Cad	T	A	5: 31,076,219 (GRCm38)	M1874K	probably benign	Het
Ccdc40	G	A	11: 119,263,189 (GRCm38)	V1096I	probably benign	Het
Ccdc93	A	G	1: 121,480,822 (GRCm38)	D358G	probably benign	Het
Cd96	T	C	16: 46,117,806 (GRCm38)	T99A	probably benign	Het
Cdc42ep3	A	T	17: 79,335,044 (GRCm38)	M149K	probably benign	Het
Cog4	A	G	8: 110,879,721 (GRCm38)	E613G	probably benign	Het
Crebbp	A	T	16: 4,084,517 (GRCm38)	I2286N	probably benign	Het
Csf1	T	C	3: 107,748,338 (GRCm38)	E459G	possibly damaging	Het
Csrp2	A	T	10: 110,935,205 (GRCm38)	Y57F	probably benign	Het
Ctdspl	C	T	9: 119,040,582 (GRCm38)	P244L	probably damaging	Het
Cyp2c29	A	G	19: 39,324,968 (GRCm38)	R303G	probably damaging	Het
Cyp4a10	C	A	4: 115,518,435 (GRCm38)	Y38*	probably null	Het
Dmgdh	T	A	13: 93,744,411 (GRCm38)	I783N	probably damaging	Het
Efcc1	G	A	6: 87,731,166 (GRCm38)	E92K	probably benign	Het
Eif2ak4	T	A	2: 118,443,210 (GRCm38)	L872H	probably damaging	Het
Elp2	T	A	18: 24,631,404 (GRCm38)	S603T	probably benign	Het
Eml2	T	A	7: 19,196,254 (GRCm38)	L300H	probably damaging	Het
Esp3	A	G	17: 40,635,936 (GRCm38)	K50R	possibly damaging	Het
Esrp1	A	G	4: 11,379,375 (GRCm38)	F136L	probably damaging	Het
Exoc1	T	A	5: 76,559,164 (GRCm38)	D497E	probably damaging	Het
Fat3	A	G	9: 15,997,465 (GRCm38)	S2414P	probably damaging	Het
Foxj3	C	T	4: 119,620,201 (GRCm38)	P369S	unknown	Het
Gkn2	G	T	6: 87,375,939 (GRCm38)		probably null	Het
Gm17333	AAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAA	AAGAAGAGAAGAGAAGAGAAGAGAAGAGAA	16: 77,852,878 (GRCm38)		noncoding transcript	Het
Gm21905	A	T	5: 67,946,381 (GRCm38)		probably benign	Het
Grhl1	T	A	12: 24,582,963 (GRCm38)		probably null	Het
Grk6	A	G	13: 55,452,154 (GRCm38)	Y221C	probably damaging	Het
Hcrtr1	T	A	4: 130,130,927 (GRCm38)	K389*	probably null	Het
Hk1	G	A	10: 62,284,784 (GRCm38)	R545C	probably damaging	Het
Hsp90b1	A	T	10: 86,696,795 (GRCm38)	I339N	probably benign	Het
Ikzf3	T	C	11: 98,490,446 (GRCm38)	R103G	probably damaging	Het
Itgad	A	C	7: 128,178,370 (GRCm38)	I141L	probably benign	Het
Jmjd6	T	A	11: 116,842,440 (GRCm38)	Y137F	probably benign	Het
Kcna1	A	G	6: 126,642,067 (GRCm38)	V430A	probably benign	Het
Kel	A	G	6: 41,688,626 (GRCm38)	V520A	probably damaging	Het
Klhl41	G	A	2: 69,670,740 (GRCm38)	V182I	probably benign	Het
Ldhb	C	A	6: 142,501,395 (GRCm38)	M64I	probably benign	Het
Lrrc23	A	G	6: 124,776,114 (GRCm38)	S190P	possibly damaging	Het
Mb21d1	A	G	9: 78,442,481 (GRCm38)	Y200H	probably damaging	Het
Mboat2	T	C	12: 24,959,030 (GRCm38)	V445A	probably benign	Het
Mlycd	G	A	8: 119,401,519 (GRCm38)	W188*	probably null	Het
Mpp3	C	T	11: 102,008,649 (GRCm38)	E349K	probably benign	Het
Myh4	A	G	11: 67,250,540 (GRCm38)	M780V	probably benign	Het
Myh6	G	A	14: 54,956,506 (GRCm38)	R809C	probably damaging	Het
Mylip	G	A	13: 45,406,570 (GRCm38)	V161M	possibly damaging	Het
Nrp1	G	A	8: 128,425,969 (GRCm38)	V220I	probably null	Het
Nup210	A	T	6: 91,034,841 (GRCm38)	N455K	probably benign	Het
Olfr116	A	T	17: 37,624,352 (GRCm38)	Y94*	probably null	Het
Olfr583	C	A	7: 103,051,588 (GRCm38)	Q97K	probably benign	Het
Pbld2	G	T	10: 63,053,953 (GRCm38)		probably null	Het
Pdlim3	A	G	8: 45,896,763 (GRCm38)	K37E	probably damaging	Het
Prkdc	T	A	16: 15,772,106 (GRCm38)	I2611N	probably benign	Het
Prr12	T	C	7: 45,028,530 (GRCm38)	D2019G	unknown	Het
Rab27a	A	G	9: 73,095,403 (GRCm38)	T205A	probably benign	Het
Rcan3	A	G	4: 135,425,284 (GRCm38)	L42P	probably damaging	Het
Rchy1	T	C	5: 91,955,615 (GRCm38)		probably null	Het
Sema6a	T	C	18: 47,248,999 (GRCm38)	H827R	probably damaging	Het
Sertad4	A	G	1: 192,850,950 (GRCm38)		probably null	Het
Smarcd1	T	A	15: 99,707,383 (GRCm38)	C282S	probably damaging	Het
Speg	A	G	1: 75,401,222 (GRCm38)	T769A	possibly damaging	Het
Syne1	T	A	10: 5,347,875 (GRCm38)	K1141*	probably null	Het
Synpo2	T	C	3: 123,117,666 (GRCm38)	E110G	probably benign	Het
Tg	T	A	15: 66,850,502 (GRCm38)	D333E	probably benign	Het
Tmem132c	A	G	5: 127,359,891 (GRCm38)	Y148C	probably damaging	Het
Tmem215	T	A	4: 40,473,965 (GRCm38)	V14E	probably damaging	Het
Tmem229b	A	G	12: 78,964,911 (GRCm38)	L82P	probably damaging	Het
Togaram1	A	G	12: 64,966,265 (GRCm38)	T97A	probably benign	Het
Trappc9	G	A	15: 72,693,548 (GRCm38)	R965*	probably null	Het
Trio	T	C	15: 27,832,985 (GRCm38)	I104V	probably benign	Het
Trp73	A	G	4: 154,063,895 (GRCm38)	F354S	probably benign	Het
Ttyh2	T	C	11: 114,686,452 (GRCm38)	L63P	probably damaging	Het
Ulk4	G	C	9: 121,044,775 (GRCm38)	P1197A	probably damaging	Het
Vmn1r176	T	C	7: 23,835,111 (GRCm38)	M206V	possibly damaging	Het
Vps8	T	A	16: 21,466,476 (GRCm38)	Y402*	probably null	Het
Zbtb41	A	G	1: 139,423,193 (GRCm38)	I15V	probably benign	Het
Zc3hav1	T	C	6: 38,307,235 (GRCm38)	I982V	possibly damaging	Het
Zmynd19	T	A	2: 24,958,111 (GRCm38)	N106K	probably benign	Het

Other mutations in Pclo

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00333:Pclo	APN	5	14,521,677 (GRCm38)	nonsense	probably null
IGL00429:Pclo	APN	5	14,680,739 (GRCm38)	unclassified	probably benign
IGL00498:Pclo	APN	5	14,540,739 (GRCm38)	missense	unknown
IGL00528:Pclo	APN	5	14,676,434 (GRCm38)	unclassified	probably benign
IGL00743:Pclo	APN	5	14,678,021 (GRCm38)	unclassified	probably benign
IGL00809:Pclo	APN	5	14,675,797 (GRCm38)	missense	unknown
IGL00811:Pclo	APN	5	14,680,010 (GRCm38)	unclassified	probably benign
IGL00819:Pclo	APN	5	14,858,846 (GRCm38)	missense	unknown
IGL00870:Pclo	APN	5	14,539,983 (GRCm38)	missense	unknown
IGL00925:Pclo	APN	5	14,766,741 (GRCm38)	missense	unknown
IGL00960:Pclo	APN	5	14,675,220 (GRCm38)	missense	unknown
IGL01013:Pclo	APN	5	14,793,834 (GRCm38)	missense	unknown
IGL01112:Pclo	APN	5	14,681,069 (GRCm38)	missense	unknown
IGL01124:Pclo	APN	5	14,714,329 (GRCm38)	missense	unknown
IGL01150:Pclo	APN	5	14,676,912 (GRCm38)	unclassified	probably benign
IGL01302:Pclo	APN	5	14,675,999 (GRCm38)	unclassified	probably benign
IGL01389:Pclo	APN	5	14,714,521 (GRCm38)	missense	probably damaging	0.99
IGL01448:Pclo	APN	5	14,676,394 (GRCm38)	unclassified	probably benign
IGL01449:Pclo	APN	5	14,678,516 (GRCm38)	unclassified	probably benign
IGL01450:Pclo	APN	5	14,677,193 (GRCm38)	unclassified	probably benign
IGL01476:Pclo	APN	5	14,521,108 (GRCm38)	missense	probably damaging	1.00
IGL01537:Pclo	APN	5	14,539,633 (GRCm38)	missense	unknown
IGL01568:Pclo	APN	5	14,678,429 (GRCm38)	unclassified	probably benign
IGL01574:Pclo	APN	5	14,713,448 (GRCm38)	missense	unknown
IGL01637:Pclo	APN	5	14,540,034 (GRCm38)	missense	unknown
IGL01646:Pclo	APN	5	14,713,867 (GRCm38)	missense	unknown
IGL01672:Pclo	APN	5	14,678,535 (GRCm38)	unclassified	probably benign
IGL01705:Pclo	APN	5	14,677,865 (GRCm38)	unclassified	probably benign
IGL02127:Pclo	APN	5	14,765,145 (GRCm38)	splice site	probably benign
IGL02215:Pclo	APN	5	14,856,985 (GRCm38)	missense	unknown
IGL02271:Pclo	APN	5	14,679,494 (GRCm38)	unclassified	probably benign
IGL02300:Pclo	APN	5	14,713,741 (GRCm38)	missense	unknown
IGL02346:Pclo	APN	5	14,677,538 (GRCm38)	unclassified	probably benign
IGL02449:Pclo	APN	5	14,515,343 (GRCm38)	missense	probably damaging	1.00
IGL02452:Pclo	APN	5	14,676,966 (GRCm38)	unclassified	probably benign
IGL02455:Pclo	APN	5	14,540,175 (GRCm38)	missense	unknown
IGL02478:Pclo	APN	5	14,766,778 (GRCm38)	missense	unknown
IGL02677:Pclo	APN	5	14,676,929 (GRCm38)	unclassified	probably benign
IGL02711:Pclo	APN	5	14,522,308 (GRCm38)	missense	unknown
IGL02737:Pclo	APN	5	14,714,163 (GRCm38)	missense	unknown
IGL02943:Pclo	APN	5	14,669,221 (GRCm38)	missense	unknown
IGL03184:Pclo	APN	5	14,714,443 (GRCm38)	missense	probably damaging	1.00
IGL03246:Pclo	APN	5	14,677,617 (GRCm38)	unclassified	probably benign
IGL03263:Pclo	APN	5	14,681,810 (GRCm38)	missense	unknown
IGL03300:Pclo	APN	5	14,712,798 (GRCm38)	missense	unknown
IGL03399:Pclo	APN	5	14,766,731 (GRCm38)	missense	unknown
Gauche	UTSW	5	14,793,855 (GRCm38)	missense	unknown
P0018:Pclo	UTSW	5	14,677,721 (GRCm38)	unclassified	probably benign
PIT4472001:Pclo	UTSW	5	14,713,168 (GRCm38)	missense	possibly damaging	0.54
R0014:Pclo	UTSW	5	14,680,451 (GRCm38)	unclassified	probably benign
R0014:Pclo	UTSW	5	14,680,451 (GRCm38)	unclassified	probably benign
R0020:Pclo	UTSW	5	14,669,673 (GRCm38)	missense	unknown
R0045:Pclo	UTSW	5	14,539,471 (GRCm38)	missense	unknown
R0046:Pclo	UTSW	5	14,540,479 (GRCm38)	missense	unknown
R0046:Pclo	UTSW	5	14,540,479 (GRCm38)	missense	unknown
R0128:Pclo	UTSW	5	14,679,797 (GRCm38)	unclassified	probably benign
R0130:Pclo	UTSW	5	14,679,797 (GRCm38)	unclassified	probably benign
R0141:Pclo	UTSW	5	14,791,922 (GRCm38)	missense	unknown
R0226:Pclo	UTSW	5	14,765,223 (GRCm38)	missense	probably damaging	0.99
R0243:Pclo	UTSW	5	14,775,420 (GRCm38)	missense	unknown
R0267:Pclo	UTSW	5	14,681,180 (GRCm38)	missense	unknown
R0313:Pclo	UTSW	5	14,678,873 (GRCm38)	unclassified	probably benign
R0324:Pclo	UTSW	5	14,669,433 (GRCm38)	missense	unknown
R0331:Pclo	UTSW	5	14,680,376 (GRCm38)	unclassified	probably benign
R0370:Pclo	UTSW	5	14,521,090 (GRCm38)	missense	probably damaging	1.00
R0398:Pclo	UTSW	5	14,681,702 (GRCm38)	missense	unknown
R0401:Pclo	UTSW	5	14,681,734 (GRCm38)	missense	unknown
R0417:Pclo	UTSW	5	14,713,022 (GRCm38)	missense	unknown
R0468:Pclo	UTSW	5	14,677,288 (GRCm38)	unclassified	probably benign
R0472:Pclo	UTSW	5	14,681,594 (GRCm38)	missense	unknown
R0488:Pclo	UTSW	5	14,669,299 (GRCm38)	missense	unknown
R0511:Pclo	UTSW	5	14,679,398 (GRCm38)	unclassified	probably benign
R0511:Pclo	UTSW	5	14,678,285 (GRCm38)	unclassified	probably benign
R0520:Pclo	UTSW	5	14,713,830 (GRCm38)	nonsense	probably null
R0547:Pclo	UTSW	5	14,792,072 (GRCm38)	missense	unknown
R0611:Pclo	UTSW	5	14,712,814 (GRCm38)	missense	unknown
R0611:Pclo	UTSW	5	14,678,775 (GRCm38)	unclassified	probably benign
R0624:Pclo	UTSW	5	14,669,656 (GRCm38)	missense	unknown
R0628:Pclo	UTSW	5	14,669,538 (GRCm38)	missense	unknown
R0639:Pclo	UTSW	5	14,681,749 (GRCm38)	nonsense	probably null
R0653:Pclo	UTSW	5	14,682,255 (GRCm38)	intron	probably benign
R0681:Pclo	UTSW	5	14,675,318 (GRCm38)	missense	unknown
R0689:Pclo	UTSW	5	14,714,019 (GRCm38)	missense	unknown
R0698:Pclo	UTSW	5	14,712,516 (GRCm38)	missense	unknown
R0737:Pclo	UTSW	5	14,515,439 (GRCm38)	missense	probably damaging	1.00
R0883:Pclo	UTSW	5	14,677,859 (GRCm38)	nonsense	probably null
R0906:Pclo	UTSW	5	14,676,686 (GRCm38)	unclassified	probably benign
R1056:Pclo	UTSW	5	14,540,055 (GRCm38)	nonsense	probably null
R1107:Pclo	UTSW	5	14,677,869 (GRCm38)	unclassified	probably benign
R1174:Pclo	UTSW	5	14,677,646 (GRCm38)	unclassified	probably benign
R1184:Pclo	UTSW	5	14,522,262 (GRCm38)	missense	unknown
R1302:Pclo	UTSW	5	14,681,633 (GRCm38)	missense	unknown
R1318:Pclo	UTSW	5	14,679,314 (GRCm38)	unclassified	probably benign
R1342:Pclo	UTSW	5	14,682,177 (GRCm38)	intron	probably benign
R1378:Pclo	UTSW	5	14,682,313 (GRCm38)	missense	probably benign	0.23
R1418:Pclo	UTSW	5	14,678,130 (GRCm38)	unclassified	probably benign
R1471:Pclo	UTSW	5	14,680,427 (GRCm38)	unclassified	probably benign
R1485:Pclo	UTSW	5	14,713,779 (GRCm38)	missense	unknown
R1523:Pclo	UTSW	5	14,788,406 (GRCm38)	missense	unknown
R1527:Pclo	UTSW	5	14,679,648 (GRCm38)	unclassified	probably benign
R1537:Pclo	UTSW	5	14,712,475 (GRCm38)	missense	unknown
R1574:Pclo	UTSW	5	14,679,831 (GRCm38)	unclassified	probably benign
R1574:Pclo	UTSW	5	14,679,831 (GRCm38)	unclassified	probably benign
R1581:Pclo	UTSW	5	14,521,282 (GRCm38)	missense	probably benign	0.28
R1613:Pclo	UTSW	5	14,679,132 (GRCm38)	unclassified	probably benign
R1632:Pclo	UTSW	5	14,680,003 (GRCm38)	unclassified	probably benign
R1688:Pclo	UTSW	5	14,788,493 (GRCm38)	critical splice donor site	probably null
R1694:Pclo	UTSW	5	14,520,963 (GRCm38)	missense	probably damaging	1.00
R1707:Pclo	UTSW	5	14,713,224 (GRCm38)	missense	unknown
R1727:Pclo	UTSW	5	14,676,987 (GRCm38)	unclassified	probably benign
R1741:Pclo	UTSW	5	14,676,510 (GRCm38)	unclassified	probably benign
R1853:Pclo	UTSW	5	14,676,684 (GRCm38)	unclassified	probably benign
R1856:Pclo	UTSW	5	14,778,552 (GRCm38)	missense	probably damaging	1.00
R1907:Pclo	UTSW	5	14,678,511 (GRCm38)	unclassified	probably benign
R1970:Pclo	UTSW	5	14,713,473 (GRCm38)	missense	unknown
R1971:Pclo	UTSW	5	14,713,473 (GRCm38)	missense	unknown
R1973:Pclo	UTSW	5	14,676,059 (GRCm38)	splice site	probably null
R1978:Pclo	UTSW	5	14,713,795 (GRCm38)	missense	unknown
R1999:Pclo	UTSW	5	14,677,080 (GRCm38)	unclassified	probably benign
R2015:Pclo	UTSW	5	14,521,501 (GRCm38)	missense	probably damaging	0.98
R2084:Pclo	UTSW	5	14,682,148 (GRCm38)	missense	probably benign	0.23
R2144:Pclo	UTSW	5	14,858,752 (GRCm38)	missense	unknown
R2155:Pclo	UTSW	5	14,714,295 (GRCm38)	missense	probably benign	0.02
R2191:Pclo	UTSW	5	14,713,848 (GRCm38)	missense	unknown
R2237:Pclo	UTSW	5	14,713,938 (GRCm38)	missense	unknown
R2276:Pclo	UTSW	5	14,714,273 (GRCm38)	missense	unknown
R2279:Pclo	UTSW	5	14,714,273 (GRCm38)	missense	unknown
R2281:Pclo	UTSW	5	14,540,332 (GRCm38)	missense	unknown
R2307:Pclo	UTSW	5	14,678,651 (GRCm38)	unclassified	probably benign
R2386:Pclo	UTSW	5	14,765,247 (GRCm38)	missense	unknown
R2407:Pclo	UTSW	5	14,678,932 (GRCm38)	unclassified	probably benign
R2512:Pclo	UTSW	5	14,712,598 (GRCm38)	missense	unknown
R2889:Pclo	UTSW	5	14,856,981 (GRCm38)	missense	unknown
R2966:Pclo	UTSW	5	14,681,150 (GRCm38)	missense	unknown
R3151:Pclo	UTSW	5	14,521,678 (GRCm38)	missense	probably damaging	0.99
R3424:Pclo	UTSW	5	14,680,418 (GRCm38)	unclassified	probably benign
R3687:Pclo	UTSW	5	14,668,995 (GRCm38)	missense	unknown
R3719:Pclo	UTSW	5	14,521,161 (GRCm38)	missense	probably benign	0.33
R3739:Pclo	UTSW	5	14,680,899 (GRCm38)	missense	unknown
R3745:Pclo	UTSW	5	14,678,421 (GRCm38)	unclassified	probably benign
R3771:Pclo	UTSW	5	14,539,408 (GRCm38)	critical splice acceptor site	probably null
R3789:Pclo	UTSW	5	14,680,450 (GRCm38)	unclassified	probably benign
R3803:Pclo	UTSW	5	14,515,402 (GRCm38)	nonsense	probably null
R3902:Pclo	UTSW	5	14,712,522 (GRCm38)	missense	probably benign	0.01
R3942:Pclo	UTSW	5	14,679,918 (GRCm38)	unclassified	probably benign
R4061:Pclo	UTSW	5	14,540,566 (GRCm38)	missense	unknown
R4094:Pclo	UTSW	5	14,855,645 (GRCm38)	missense	unknown
R4175:Pclo	UTSW	5	14,713,875 (GRCm38)	missense	probably damaging	0.99
R4195:Pclo	UTSW	5	14,677,563 (GRCm38)	unclassified	probably benign
R4398:Pclo	UTSW	5	14,775,366 (GRCm38)	missense	probably damaging	1.00
R4429:Pclo	UTSW	5	14,678,100 (GRCm38)	unclassified	probably benign
R4523:Pclo	UTSW	5	14,679,992 (GRCm38)	unclassified	probably benign
R4531:Pclo	UTSW	5	14,775,408 (GRCm38)	missense	unknown
R4552:Pclo	UTSW	5	14,669,271 (GRCm38)	missense	unknown
R4563:Pclo	UTSW	5	14,521,369 (GRCm38)	missense	probably damaging	1.00
R4581:Pclo	UTSW	5	14,675,505 (GRCm38)	missense	unknown
R4638:Pclo	UTSW	5	14,680,433 (GRCm38)	nonsense	probably null
R4655:Pclo	UTSW	5	14,682,383 (GRCm38)	intron	probably benign
R4703:Pclo	UTSW	5	14,676,480 (GRCm38)	unclassified	probably benign
R4704:Pclo	UTSW	5	14,676,480 (GRCm38)	unclassified	probably benign
R4705:Pclo	UTSW	5	14,676,480 (GRCm38)	unclassified	probably benign
R4706:Pclo	UTSW	5	14,714,207 (GRCm38)	missense	unknown
R4709:Pclo	UTSW	5	14,778,558 (GRCm38)	missense	unknown
R4755:Pclo	UTSW	5	14,714,348 (GRCm38)	missense	unknown
R4786:Pclo	UTSW	5	14,723,267 (GRCm38)	missense	unknown
R4801:Pclo	UTSW	5	14,675,815 (GRCm38)	missense	unknown
R4802:Pclo	UTSW	5	14,675,815 (GRCm38)	missense	unknown
R4812:Pclo	UTSW	5	14,540,025 (GRCm38)	missense	unknown
R4817:Pclo	UTSW	5	14,713,125 (GRCm38)	missense	unknown
R4817:Pclo	UTSW	5	14,675,031 (GRCm38)	missense	unknown
R4845:Pclo	UTSW	5	14,679,118 (GRCm38)	unclassified	probably benign
R4876:Pclo	UTSW	5	14,811,680 (GRCm38)	missense	unknown
R4907:Pclo	UTSW	5	14,680,051 (GRCm38)	unclassified	probably benign
R4943:Pclo	UTSW	5	14,712,637 (GRCm38)	missense	unknown
R4963:Pclo	UTSW	5	14,669,221 (GRCm38)	missense	unknown
R4970:Pclo	UTSW	5	14,677,882 (GRCm38)	unclassified	probably benign
R4978:Pclo	UTSW	5	14,714,478 (GRCm38)	missense	probably benign	0.23
R4982:Pclo	UTSW	5	14,679,294 (GRCm38)	unclassified	probably benign
R5019:Pclo	UTSW	5	14,714,367 (GRCm38)	missense	unknown
R5068:Pclo	UTSW	5	14,679,073 (GRCm38)	unclassified	probably benign
R5092:Pclo	UTSW	5	14,677,308 (GRCm38)	unclassified	probably benign
R5112:Pclo	UTSW	5	14,677,882 (GRCm38)	unclassified	probably benign
R5124:Pclo	UTSW	5	14,677,392 (GRCm38)	unclassified	probably benign
R5210:Pclo	UTSW	5	14,713,450 (GRCm38)	missense	probably damaging	0.99
R5227:Pclo	UTSW	5	14,713,560 (GRCm38)	missense	probably benign	0.23
R5264:Pclo	UTSW	5	14,676,923 (GRCm38)	unclassified	probably benign
R5280:Pclo	UTSW	5	14,540,717 (GRCm38)	missense	unknown
R5286:Pclo	UTSW	5	14,679,747 (GRCm38)	unclassified	probably benign
R5297:Pclo	UTSW	5	14,676,249 (GRCm38)	unclassified	probably benign
R5344:Pclo	UTSW	5	14,676,612 (GRCm38)	unclassified	probably benign
R5354:Pclo	UTSW	5	14,678,808 (GRCm38)	unclassified	probably benign
R5358:Pclo	UTSW	5	14,712,736 (GRCm38)	nonsense	probably null
R5363:Pclo	UTSW	5	14,669,410 (GRCm38)	missense	unknown
R5377:Pclo	UTSW	5	14,681,353 (GRCm38)	missense	unknown
R5457:Pclo	UTSW	5	14,676,143 (GRCm38)	unclassified	probably benign
R5468:Pclo	UTSW	5	14,680,952 (GRCm38)	missense	unknown
R5588:Pclo	UTSW	5	14,788,398 (GRCm38)	missense	unknown
R5836:Pclo	UTSW	5	14,678,535 (GRCm38)	unclassified	probably benign
R5865:Pclo	UTSW	5	14,714,478 (GRCm38)	missense	probably benign	0.23
R5875:Pclo	UTSW	5	14,680,600 (GRCm38)	unclassified	probably benign
R5892:Pclo	UTSW	5	14,521,171 (GRCm38)	missense	probably damaging	1.00
R5905:Pclo	UTSW	5	14,680,385 (GRCm38)	unclassified	probably benign
R5967:Pclo	UTSW	5	14,540,655 (GRCm38)	missense	unknown
R6046:Pclo	UTSW	5	14,713,288 (GRCm38)	missense	unknown
R6059:Pclo	UTSW	5	14,811,700 (GRCm38)	missense	unknown
R6092:Pclo	UTSW	5	14,677,923 (GRCm38)	unclassified	probably benign
R6119:Pclo	UTSW	5	14,677,019 (GRCm38)	unclassified	probably benign
R6221:Pclo	UTSW	5	14,675,313 (GRCm38)	missense	unknown
R6243:Pclo	UTSW	5	14,676,443 (GRCm38)	unclassified	probably benign
R6269:Pclo	UTSW	5	14,522,094 (GRCm38)	nonsense	probably null
R6303:Pclo	UTSW	5	14,677,893 (GRCm38)	unclassified	probably benign
R6304:Pclo	UTSW	5	14,677,893 (GRCm38)	unclassified	probably benign
R6453:Pclo	UTSW	5	14,676,789 (GRCm38)	unclassified	probably benign
R6460:Pclo	UTSW	5	14,679,132 (GRCm38)	unclassified	probably benign
R6497:Pclo	UTSW	5	14,793,855 (GRCm38)	missense	unknown
R6498:Pclo	UTSW	5	14,669,491 (GRCm38)	missense	unknown
R6524:Pclo	UTSW	5	14,718,869 (GRCm38)	missense	unknown
R6682:Pclo	UTSW	5	14,539,879 (GRCm38)	missense	unknown
R6823:Pclo	UTSW	5	14,677,907 (GRCm38)	unclassified	probably benign
R6830:Pclo	UTSW	5	14,681,099 (GRCm38)	missense	unknown
R6831:Pclo	UTSW	5	14,788,429 (GRCm38)	nonsense	probably null
R6965:Pclo	UTSW	5	14,681,962 (GRCm38)	intron	probably benign
R7012:Pclo	UTSW	5	14,750,479 (GRCm38)	missense	unknown
R7028:Pclo	UTSW	5	14,713,447 (GRCm38)	missense	unknown
R7030:Pclo	UTSW	5	14,676,407 (GRCm38)	missense	probably benign	0.23
R7130:Pclo	UTSW	5	14,679,342 (GRCm38)	missense	unknown
R7141:Pclo	UTSW	5	14,679,257 (GRCm38)	missense	unknown
R7143:Pclo	UTSW	5	14,858,822 (GRCm38)	missense	unknown
R7189:Pclo	UTSW	5	14,521,918 (GRCm38)	missense	possibly damaging	0.83
R7190:Pclo	UTSW	5	14,679,729 (GRCm38)	missense	unknown
R7273:Pclo	UTSW	5	14,681,594 (GRCm38)	missense	unknown
R7341:Pclo	UTSW	5	14,675,836 (GRCm38)	missense	unknown
R7361:Pclo	UTSW	5	14,793,868 (GRCm38)	missense	probably damaging	0.99
R7390:Pclo	UTSW	5	14,682,010 (GRCm38)	missense	unknown
R7396:Pclo	UTSW	5	14,539,888 (GRCm38)	missense	unknown
R7428:Pclo	UTSW	5	14,750,517 (GRCm38)	missense
R7448:Pclo	UTSW	5	14,669,617 (GRCm38)	missense	unknown
R7476:Pclo	UTSW	5	14,521,331 (GRCm38)	missense	probably damaging	1.00
R7483:Pclo	UTSW	5	14,712,592 (GRCm38)	missense
R7524:Pclo	UTSW	5	14,678,303 (GRCm38)	missense	unknown
R7526:Pclo	UTSW	5	14,521,062 (GRCm38)	missense	probably benign	0.11
R7537:Pclo	UTSW	5	14,682,104 (GRCm38)	missense	unknown
R7597:Pclo	UTSW	5	14,858,855 (GRCm38)	missense	unknown
R7597:Pclo	UTSW	5	14,677,587 (GRCm38)	missense	unknown
R7605:Pclo	UTSW	5	14,679,036 (GRCm38)	missense	unknown
R7646:Pclo	UTSW	5	14,520,895 (GRCm38)	missense	probably damaging	1.00
R7685:Pclo	UTSW	5	14,680,616 (GRCm38)	missense	unknown
R7879:Pclo	UTSW	5	14,677,214 (GRCm38)	missense	unknown
R7885:Pclo	UTSW	5	14,714,195 (GRCm38)	frame shift	probably null
R7885:Pclo	UTSW	5	14,714,191 (GRCm38)	frame shift	probably null
R7885:Pclo	UTSW	5	14,714,190 (GRCm38)	frame shift	probably null
R7887:Pclo	UTSW	5	14,714,190 (GRCm38)	frame shift	probably null
R7921:Pclo	UTSW	5	14,669,234 (GRCm38)	missense	unknown
R7948:Pclo	UTSW	5	14,765,166 (GRCm38)	nonsense	probably null
R7970:Pclo	UTSW	5	14,714,478 (GRCm38)	missense
R8021:Pclo	UTSW	5	14,539,784 (GRCm38)	missense	unknown
R8034:Pclo	UTSW	5	14,521,200 (GRCm38)	missense	probably damaging	0.99
R8079:Pclo	UTSW	5	14,540,458 (GRCm38)	missense	unknown
R8092:Pclo	UTSW	5	14,677,546 (GRCm38)	missense	unknown
R8096:Pclo	UTSW	5	14,712,961 (GRCm38)	missense
R8182:Pclo	UTSW	5	14,855,620 (GRCm38)	missense	unknown
R8188:Pclo	UTSW	5	14,675,179 (GRCm38)	missense	unknown
R8218:Pclo	UTSW	5	14,675,663 (GRCm38)	missense	unknown
R8288:Pclo	UTSW	5	14,712,871 (GRCm38)	missense
R8330:Pclo	UTSW	5	14,675,297 (GRCm38)	missense	unknown
R8382:Pclo	UTSW	5	14,677,066 (GRCm38)	missense	unknown
R8447:Pclo	UTSW	5	14,681,409 (GRCm38)	missense
R8491:Pclo	UTSW	5	14,515,230 (GRCm38)	missense	unknown
R8506:Pclo	UTSW	5	14,540,745 (GRCm38)	missense	unknown
R8524:Pclo	UTSW	5	14,679,507 (GRCm38)	unclassified	probably benign
R8548:Pclo	UTSW	5	14,682,254 (GRCm38)	splice site	probably null
R8670:Pclo	UTSW	5	14,682,047 (GRCm38)	missense	unknown
R8691:Pclo	UTSW	5	14,521,692 (GRCm38)	missense	unknown
R8719:Pclo	UTSW	5	14,713,764 (GRCm38)	nonsense	probably null
R8743:Pclo	UTSW	5	14,714,566 (GRCm38)	missense
R8756:Pclo	UTSW	5	14,714,384 (GRCm38)	missense
R8829:Pclo	UTSW	5	14,788,450 (GRCm38)	missense
R8832:Pclo	UTSW	5	14,788,450 (GRCm38)	missense
R8912:Pclo	UTSW	5	14,775,321 (GRCm38)	missense
R8930:Pclo	UTSW	5	14,712,975 (GRCm38)	missense	possibly damaging	0.95
R8932:Pclo	UTSW	5	14,712,975 (GRCm38)	missense	possibly damaging	0.95
R8958:Pclo	UTSW	5	14,713,042 (GRCm38)	missense	unknown
R8958:Pclo	UTSW	5	14,675,355 (GRCm38)	missense	unknown
R8969:Pclo	UTSW	5	14,522,194 (GRCm38)	missense	unknown
R8989:Pclo	UTSW	5	14,669,311 (GRCm38)	missense	unknown
R8991:Pclo	UTSW	5	14,669,311 (GRCm38)	missense	unknown
R8992:Pclo	UTSW	5	14,669,311 (GRCm38)	missense	unknown
R8998:Pclo	UTSW	5	14,677,496 (GRCm38)	missense	unknown
R9013:Pclo	UTSW	5	14,677,613 (GRCm38)	missense	unknown
R9035:Pclo	UTSW	5	14,713,178 (GRCm38)	missense
R9060:Pclo	UTSW	5	14,676,585 (GRCm38)	missense	unknown
R9122:Pclo	UTSW	5	14,679,984 (GRCm38)	missense	unknown
R9164:Pclo	UTSW	5	14,681,685 (GRCm38)	missense
R9170:Pclo	UTSW	5	14,681,054 (GRCm38)	missense
R9192:Pclo	UTSW	5	14,540,190 (GRCm38)	missense	unknown
R9203:Pclo	UTSW	5	14,678,514 (GRCm38)	missense	unknown
R9245:Pclo	UTSW	5	14,676,945 (GRCm38)	missense	unknown
R9260:Pclo	UTSW	5	14,714,273 (GRCm38)	missense	unknown
R9336:Pclo	UTSW	5	14,676,548 (GRCm38)	missense	unknown
R9378:Pclo	UTSW	5	14,765,863 (GRCm38)	missense
R9454:Pclo	UTSW	5	14,712,438 (GRCm38)	missense	probably benign	0.01
R9462:Pclo	UTSW	5	14,790,394 (GRCm38)	missense
R9464:Pclo	UTSW	5	14,766,816 (GRCm38)	missense
R9474:Pclo	UTSW	5	14,521,236 (GRCm38)	missense	possibly damaging	0.78
R9500:Pclo	UTSW	5	14,675,634 (GRCm38)	missense	unknown
R9528:Pclo	UTSW	5	14,677,677 (GRCm38)	missense	unknown
R9567:Pclo	UTSW	5	14,713,422 (GRCm38)	missense
R9569:Pclo	UTSW	5	14,857,051 (GRCm38)	critical splice donor site	probably null
R9578:Pclo	UTSW	5	14,766,739 (GRCm38)	missense
R9647:Pclo	UTSW	5	14,681,774 (GRCm38)	missense
R9673:Pclo	UTSW	5	14,858,744 (GRCm38)	critical splice acceptor site	probably null
R9691:Pclo	UTSW	5	14,677,544 (GRCm38)	missense	unknown
R9706:Pclo	UTSW	5	14,855,669 (GRCm38)	missense	unknown
R9803:Pclo	UTSW	5	14,712,615 (GRCm38)	missense
RF015:Pclo	UTSW	5	14,515,269 (GRCm38)	missense	unknown
X0058:Pclo	UTSW	5	14,682,131 (GRCm38)	missense	probably benign	0.23
Z1176:Pclo	UTSW	5	14,712,648 (GRCm38)	missense	probably damaging	1.00
Z1176:Pclo	UTSW	5	14,681,779 (GRCm38)	missense
Z1176:Pclo	UTSW	5	14,681,516 (GRCm38)	missense	possibly damaging	0.95
Z1177:Pclo	UTSW	5	14,522,013 (GRCm38)	missense	possibly damaging	0.68
Z1177:Pclo	UTSW	5	14,522,008 (GRCm38)	missense	probably damaging	0.99
Z1177:Pclo	UTSW	5	14,678,156 (GRCm38)	missense	unknown

Predicted Primers

PCR Primer
(F):5'- AACTCCAGCTCAGCTATCTGGACC -3'
(R):5'- GCTACACACGGTGCTTTGACACTC -3'

Sequencing Primer
(F):5'- TCAGCTATCTGGACCTGGAAAAAC -3'
(R):5'- CCTTTTCTGGAGTATGCAACAG -3'

Posted On

2014-04-13