Mutagenetix > Incidental Mutations

Incidental Mutation 'R1528:Olfr675'

166362

Institutional Source

Beutler Lab

Gene Symbol

Olfr675

Ensembl Gene

ENSMUSG00000096773

Gene Name

olfactory receptor 675

Synonyms

GA_x6K02T2PBJ9-7653782-7652841, MOR32-9P

MMRRC Submission

039568-MU

Accession Numbers

Is this an essential gene?

Possibly non essential (E-score: 0.335) question?

Stock #

R1528 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

105021370-105028460 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 105024764 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Leucine to Glutamine at position 72 (L72Q)

Ref Sequence

ENSEMBL: ENSMUSP00000149895 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000073102] [ENSMUST00000210113] [ENSMUST00000214318] [ENSMUST00000215899]

AlphaFold

A0A1B0GSE1

Predicted Effect

probably damaging
Transcript: ENSMUST00000073102
AA Change: L68Q

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000072847
Gene: ENSMUSG00000096773
AA Change: L68Q

Domain	Start	End	E-Value	Type
Pfam:7tm_4	33	311	5e-118	PFAM
Pfam:7TM_GPCR_Srsx	37	308	1.9e-6	PFAM
Pfam:7tm_1	43	293	2.6e-17	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000210113
AA Change: L72Q

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

Predicted Effect

probably damaging
Transcript: ENSMUST00000214318
AA Change: L72Q

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

Predicted Effect

probably damaging
Transcript: ENSMUST00000215899
AA Change: L72Q

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

Coding Region Coverage

1x: 99.4%
3x: 98.7%
10x: 97.3%
20x: 95.4%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 83 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2610008E11Rik	T	A	10: 79,067,696	Q262L	possibly damaging	Het
4921501E09Rik	A	G	17: 33,067,241	S196P	probably damaging	Het
4932438A13Rik	C	T	3: 37,052,535	H5005Y	unknown	Het
Abcg4	T	C	9: 44,274,723	Y617C	probably damaging	Het
Amph	G	A	13: 19,142,028	V643M	probably damaging	Het
Ano1	T	G	7: 144,595,566	S853R	probably damaging	Het
Ap4e1	T	A	2: 127,011,823	S60R	possibly damaging	Het
Atp4b	C	A	8: 13,389,693	K176N	possibly damaging	Het
Atxn2	A	T	5: 121,802,108	D982V	probably damaging	Het
Atxn2	T	C	5: 121,813,530	F646S	probably damaging	Het
Bcl2l13	T	C	6: 120,870,794	C136R	possibly damaging	Het
Cacna1i	A	G	15: 80,391,774		probably null	Het
Ccdc122	T	A	14: 77,067,939	V11D	possibly damaging	Het
Cdnf	A	G	2: 3,521,041	D90G	probably damaging	Het
Cep104	T	G	4: 153,994,508	V323G	probably benign	Het
Chmp6	A	G	11: 119,916,715	D128G	probably benign	Het
Clec18a	A	T	8: 111,078,866	M201K	probably benign	Het
Col11a1	A	T	3: 114,216,995		probably benign	Het
Col6a4	A	T	9: 106,075,220	M493K	probably damaging	Het
Crygd	A	C	1: 65,063,057		probably null	Het
Dennd1c	T	C	17: 57,066,935	T543A	probably benign	Het
Erbb4	A	T	1: 68,078,582	C891*	probably null	Het
Ercc5	A	T	1: 44,178,241	K915*	probably null	Het
Ercc6	A	T	14: 32,519,022	N168Y	probably damaging	Het
Esrp2	G	T	8: 106,136,752	P6T	unknown	Het
Exoc1	A	G	5: 76,549,564	K396R	possibly damaging	Het
Fat3	T	A	9: 15,925,091	Y4039F	probably benign	Het
Fgf7	T	A	2: 126,035,818	M35K	probably damaging	Het
Fras1	G	A	5: 96,636,819	G887D	probably damaging	Het
Fuk	A	T	8: 110,883,241	L1047Q	probably damaging	Het
Gbp4	A	T	5: 105,121,792		probably null	Het
Homez	A	T	14: 54,857,705	M182K	probably benign	Het
Hrnr	G	A	3: 93,322,794	S113N	possibly damaging	Het
Ifit3b	T	G	19: 34,611,672	S83A	probably benign	Het
Ildr2	A	G	1: 166,270,495		probably null	Het
Klhdc1	A	T	12: 69,263,198	R291S	probably benign	Het
Krt77	T	A	15: 101,861,088	I413F	probably damaging	Het
Lipn	A	G	19: 34,068,670	I14M	probably damaging	Het
Macf1	T	G	4: 123,476,014	R86S	probably benign	Het
Mroh8	C	A	2: 157,230,055	G510V	probably damaging	Het
Mycbp2	A	T	14: 103,232,597	D1255E	possibly damaging	Het
Nckap5	C	T	1: 126,024,922	V1234I	possibly damaging	Het
Nlrp9c	T	C	7: 26,382,298	K668E	probably damaging	Het
Nod2	T	C	8: 88,664,589	M508T	possibly damaging	Het
Npffr1	A	G	10: 61,614,237	M97V	possibly damaging	Het
Nsd3	G	A	8: 25,698,767	V43M	probably damaging	Het
Nubp2	A	G	17: 24,884,414	V163A	probably damaging	Het
Oas1e	A	T	5: 120,787,989	F338Y	probably damaging	Het
Oat	C	A	7: 132,564,269	G196C	probably damaging	Het
Olfr1241	C	T	2: 89,482,553	G194D	probably damaging	Het
Olfr57	T	C	10: 79,035,564	L256P	probably damaging	Het
P2ry13	T	C	3: 59,210,289	T23A	probably benign	Het
Pja2	C	A	17: 64,309,222	S226I	possibly damaging	Het
Pkhd1l1	T	C	15: 44,526,724	V1412A	probably damaging	Het
Plekhs1	T	C	19: 56,479,995	S332P	probably damaging	Het
Polr3e	T	A	7: 120,940,597	N522K	probably damaging	Het
Prdm10	A	G	9: 31,357,286	T844A	probably damaging	Het
Ripk1	C	T	13: 34,028,147	P480L	probably benign	Het
Rnf139	T	C	15: 58,899,215	V363A	probably damaging	Het
Rnf19a	A	T	15: 36,265,655	S99T	possibly damaging	Het
Rnf224	G	A	2: 25,236,098	T81I	probably benign	Het
Rpgrip1	T	C	14: 52,112,224	L23S	probably benign	Het
Setd5	T	A	6: 113,121,738	F758L	probably damaging	Het
Smlr1	C	A	10: 25,536,078	V4L	possibly damaging	Het
Snx1	T	C	9: 66,109,543	D34G	probably damaging	Het
Spaca5	A	T	X: 21,076,653	T92S	probably benign	Het
Speer4f2	A	G	5: 17,376,542	T161A		Het
Swi5	C	A	2: 32,280,704		probably null	Het
Syne2	T	A	12: 75,966,100	D2689E	probably benign	Het
Tcof1	T	A	18: 60,814,999	K1299*	probably null	Het
Tmprss11e	G	A	5: 86,724,210	T49I	probably damaging	Het
Tmx3	T	A	18: 90,537,086	V309D	possibly damaging	Het
Trim30b	C	G	7: 104,357,299	V117L	possibly damaging	Het
Tsen2	C	A	6: 115,560,028	H248Q	probably benign	Het
Ttn	T	C	2: 76,737,068	Y19500C	probably damaging	Het
Tubgcp2	A	G	7: 140,033,783		probably benign	Het
Vwf	A	C	6: 125,608,291	D712A	possibly damaging	Het
Wfdc2	A	G	2: 164,565,908	K166E	probably damaging	Het
Xrcc2	T	C	5: 25,692,294	D219G	probably benign	Het
Zc3h6	C	T	2: 129,017,069	P1007S	probably benign	Het
Zdhhc17	A	T	10: 110,948,189		probably null	Het
Zfp369	T	A	13: 65,292,165	I221N	probably damaging	Het
Zfp655	A	T	5: 145,244,601	N423I	probably damaging	Het

Other mutations in Olfr675

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02639:Olfr675	APN	7	105024222	missense	probably damaging	1.00
IGL02944:Olfr675	APN	7	105024923	missense	probably damaging	1.00
R1182:Olfr675	UTSW	7	105024078	missense	probably damaging	1.00
R1412:Olfr675	UTSW	7	105024195	missense	probably damaging	1.00
R1555:Olfr675	UTSW	7	105024522	missense	probably benign	0.00
R1589:Olfr675	UTSW	7	105024560	missense	probably benign
R1778:Olfr675	UTSW	7	105024163	missense	probably benign	0.03
R3690:Olfr675	UTSW	7	105024695	missense	probably damaging	0.99
R3848:Olfr675	UTSW	7	105024332	missense	probably damaging	0.99
R4784:Olfr675	UTSW	7	105024530	missense	probably damaging	0.97
R5050:Olfr675	UTSW	7	105024387	missense	probably damaging	1.00
R5074:Olfr675	UTSW	7	105024053	missense	probably benign
R5499:Olfr675	UTSW	7	105024977	start codon destroyed	probably null	0.06
R5586:Olfr675	UTSW	7	105024221	missense	probably damaging	1.00
R7244:Olfr675	UTSW	7	105024941	missense	probably benign
R8297:Olfr675	UTSW	7	105024678	missense	probably benign	0.14
R8532:Olfr675	UTSW	7	105024566	missense	probably damaging	1.00
R9087:Olfr675	UTSW	7	105024703	nonsense	probably null
Z1176:Olfr675	UTSW	7	105024099	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- ACCATGTATAGGCTCCTCAGCACAG -3'
(R):5'- CATCCCAGGGCTAGAATATGCACAC -3'

Sequencing Primer
(F):5'- TGCAATGACCCCAATGACTTTG -3'
(R):5'- GGCTAGAATATGCACACATCTG -3'

Posted On

2014-04-13