Mutagenetix > Incidental Mutation

Incidental Mutation 'R1535:Vmn2r8'

166880

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r8

Ensembl Gene

ENSMUSG00000090961

Gene Name

vomeronasal 2, receptor 8

Synonyms

EG627479

MMRRC Submission

039574-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.069) question?

Stock #

R1535 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

108797193-108808754 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 108802174 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Cysteine at position 269 (Y269C)

Ref Sequence

ENSEMBL: ENSMUSP00000126953 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000172140]

AlphaFold

L7N472

Predicted Effect

probably damaging
Transcript: ENSMUST00000172140
AA Change: Y269C

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000126953
Gene: ENSMUSG00000090961
AA Change: Y269C

Domain	Start	End	E-Value	Type
signal peptide	1	21	N/A	INTRINSIC
Pfam:ANF_receptor	78	419	1.1e-28	PFAM
Pfam:NCD3G	507	561	8.2e-18	PFAM
Pfam:7tm_3	594	829	1.1e-54	PFAM

Meta Mutation Damage Score

0.6467

Coding Region Coverage

1x: 99.2%
3x: 98.3%
10x: 96.4%
20x: 93.2%

Validation Efficiency

100% (75/75)

Allele List at MGI

Other mutations in this stock

Total: 71 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1110032A03Rik	C	T	9: 50,764,007	R40Q	probably damaging	Het
3110009E18Rik	T	C	1: 120,171,473	S99P	possibly damaging	Het
4932431P20Rik	C	T	7: 29,529,579		noncoding transcript	Het
Abca16	T	A	7: 120,540,705	I1530N	probably benign	Het
Abcc9	T	G	6: 142,664,635	Q601P	probably damaging	Het
Acap3	A	G	4: 155,896,174		probably benign	Het
Adamts16	A	T	13: 70,791,794		probably null	Het
Alpk2	G	C	18: 65,350,204	S244R	probably benign	Het
Atp8b1	C	T	18: 64,545,264	V854M	probably damaging	Het
Bend5	C	A	4: 111,459,763	D374E	probably benign	Het
Catsperg2	T	C	7: 29,698,246	D1014G	possibly damaging	Het
Ccer2	G	T	7: 28,757,397	G189*	probably null	Het
Ces2h	A	G	8: 105,014,486	T37A	probably benign	Het
Cmah	T	C	13: 24,439,220	F213L	probably damaging	Het
Cntn2	T	C	1: 132,525,384	E390G	probably benign	Het
Coq7	C	T	7: 118,529,674	R46Q	possibly damaging	Het
Cpb1	T	A	3: 20,266,287	D136V	probably benign	Het
Cxcr2	T	C	1: 74,159,058	L237P	probably damaging	Het
Daam1	T	C	12: 71,951,918	L549P	unknown	Het
Diaph1	A	G	18: 37,896,093		probably null	Het
Dis3	G	T	14: 99,079,426	Q825K	probably damaging	Het
Dnah11	C	T	12: 118,018,730	R2568Q	probably damaging	Het
Dnah12	A	T	14: 26,816,322	R2312S	possibly damaging	Het
Dock9	C	T	14: 121,546,064	E2041K	probably damaging	Het
Doxl2	T	C	6: 48,975,464	F108L	probably damaging	Het
Eci1	A	G	17: 24,439,090	E282G	probably benign	Het
Egf	T	A	3: 129,690,778	T464S	probably benign	Het
Eif4g3	C	T	4: 138,097,302	P97S	probably damaging	Het
Emsy	A	G	7: 98,593,737	V1065A	possibly damaging	Het
Ep400	G	T	5: 110,708,166		probably benign	Het
Fam13a	A	C	6: 58,939,347	I607S	probably damaging	Het
Fam241b	A	G	10: 62,108,949	S113P	probably damaging	Het
Fbxl21	A	T	13: 56,523,527	M1L	probably benign	Het
Fnip2	C	A	3: 79,481,765	C553F	probably damaging	Het
Frmd3	G	T	4: 74,013,758		probably benign	Het
Gm597	T	C	1: 28,777,424	E509G	probably damaging	Het
Gm9745	A	T	13: 8,940,644		probably benign	Het
Grm4	G	A	17: 27,434,801	S470L	probably benign	Het
Gucy2e	T	A	11: 69,226,244	I784F	probably damaging	Het
H1fx	T	C	6: 87,981,148	Y70C	probably damaging	Het
Hmcn2	A	T	2: 31,420,407	T3338S	possibly damaging	Het
Idh1	C	T	1: 65,168,538	G161R	probably damaging	Het
Idi1	T	C	13: 8,886,909		probably benign	Het
Kcnq2	C	T	2: 181,134,825	G90S	probably damaging	Het
Klhl29	A	G	12: 5,084,486	Y642H	probably damaging	Het
Lmtk3	T	A	7: 45,794,570		probably benign	Het
Macf1	A	T	4: 123,440,693	F4420I	probably benign	Het
Me1	A	G	9: 86,587,043	S470P	probably damaging	Het
Mfsd2b	A	G	12: 4,870,605	S71P	probably damaging	Het
Mfsd3	T	C	15: 76,702,779	L273P	probably damaging	Het
Mical1	A	G	10: 41,485,211	K757R	possibly damaging	Het
Myh9	A	T	15: 77,777,813	V782D	probably damaging	Het
Nab2	C	G	10: 127,665,047	E59Q	probably damaging	Het
Nfyc	A	T	4: 120,761,724	N244K	probably damaging	Het
Nupr1l	A	G	5: 129,908,831	I80M	probably damaging	Het
Olfr1229	T	C	2: 89,283,057	I46M	probably benign	Het
Olfr583	A	G	7: 103,051,369	T24A	probably benign	Het
Olfr76	A	G	19: 12,120,332	C115R	probably damaging	Het
Pkn3	A	G	2: 30,087,053	T602A	probably benign	Het
Prrc2b	T	A	2: 32,204,289	V561D	probably benign	Het
Psd2	T	A	18: 36,005,790	V489E	probably benign	Het
Rasal2	T	C	1: 157,230,059	T181A	probably benign	Het
Serpinb13	T	C	1: 106,982,156	M1T	probably null	Het
Sgsm1	A	T	5: 113,263,269	S864T	possibly damaging	Het
Slc5a10	C	A	11: 61,673,941	G404V	possibly damaging	Het
Slc6a1	G	T	6: 114,307,770	G263V	probably damaging	Het
Son	A	G	16: 91,659,734	K1790E	probably damaging	Het
Syde2	A	G	3: 146,002,421		probably benign	Het
Tmem126a	T	C	7: 90,452,818	I58V	probably benign	Het
Yeats2	T	A	16: 20,189,365	S416T	probably damaging	Het
Zfp40	A	T	17: 23,175,869	H513Q	probably damaging	Het

Other mutations in Vmn2r8

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02957:Vmn2r8	APN	5	108802225	missense	probably benign	0.01
R0324:Vmn2r8	UTSW	5	108797941	splice site	probably null
R0335:Vmn2r8	UTSW	5	108797451	splice site	probably null
R0394:Vmn2r8	UTSW	5	108802072	missense	probably benign	0.12
R0501:Vmn2r8	UTSW	5	108803183	missense	probably benign	0.03
R0615:Vmn2r8	UTSW	5	108799329	missense	probably damaging	1.00
R0678:Vmn2r8	UTSW	5	108800546	missense	probably benign	0.00
R1167:Vmn2r8	UTSW	5	108803176	missense	probably benign	0.01
R1187:Vmn2r8	UTSW	5	108803219	nonsense	probably null
R1406:Vmn2r8	UTSW	5	108802368	missense	probably benign
R1406:Vmn2r8	UTSW	5	108802368	missense	probably benign
R1451:Vmn2r8	UTSW	5	108798067	missense	probably damaging	1.00
R1795:Vmn2r8	UTSW	5	108803106	missense	probably benign
R1874:Vmn2r8	UTSW	5	108802418	missense	possibly damaging	0.74
R1908:Vmn2r8	UTSW	5	108797570	missense	probably benign	0.03
R1925:Vmn2r8	UTSW	5	108802153	missense	probably damaging	0.97
R1960:Vmn2r8	UTSW	5	108799286	missense	probably damaging	0.99
R1961:Vmn2r8	UTSW	5	108798095	missense	probably benign	0.45
R1967:Vmn2r8	UTSW	5	108802383	missense	probably benign	0.01
R2095:Vmn2r8	UTSW	5	108808621	missense	possibly damaging	0.94
R2159:Vmn2r8	UTSW	5	108802303	missense	probably benign	0.22
R4240:Vmn2r8	UTSW	5	108797503	missense	probably damaging	0.99
R4581:Vmn2r8	UTSW	5	108801704	missense	probably benign	0.03
R4744:Vmn2r8	UTSW	5	108808581	missense	probably benign	0.00
R4755:Vmn2r8	UTSW	5	108801700	missense	probably benign	0.03
R4917:Vmn2r8	UTSW	5	108797398	missense	probably damaging	1.00
R4957:Vmn2r8	UTSW	5	108799263	missense	probably benign	0.16
R5141:Vmn2r8	UTSW	5	108808706	missense	probably damaging	0.96
R5481:Vmn2r8	UTSW	5	108801770	missense	probably benign	0.09
R5571:Vmn2r8	UTSW	5	108802240	missense	probably damaging	1.00
R5624:Vmn2r8	UTSW	5	108802459	missense	probably damaging	0.99
R6003:Vmn2r8	UTSW	5	108797382	missense	probably damaging	1.00
R6243:Vmn2r8	UTSW	5	108799345	missense	probably benign	0.01
R6265:Vmn2r8	UTSW	5	108808597	missense	probably benign
R6315:Vmn2r8	UTSW	5	108801891	missense	probably benign
R6413:Vmn2r8	UTSW	5	108801723	missense	probably benign	0.09
R7120:Vmn2r8	UTSW	5	108808638	missense	possibly damaging	0.56
R7406:Vmn2r8	UTSW	5	108800576	missense	probably benign	0.00
R7409:Vmn2r8	UTSW	5	108808583	nonsense	probably null
R7489:Vmn2r8	UTSW	5	108797656	missense	possibly damaging	0.95
R7532:Vmn2r8	UTSW	5	108802240	missense	probably benign	0.22
R7534:Vmn2r8	UTSW	5	108802174	missense	possibly damaging	0.94
R7739:Vmn2r8	UTSW	5	108802177	missense	probably damaging	1.00
R8099:Vmn2r8	UTSW	5	108801834	missense	probably benign
R8245:Vmn2r8	UTSW	5	108798070	missense	probably damaging	1.00
R8711:Vmn2r8	UTSW	5	108798096	missense	possibly damaging	0.89
R8781:Vmn2r8	UTSW	5	108797731	missense	possibly damaging	0.95
R8874:Vmn2r8	UTSW	5	108808751	missense	probably damaging	1.00
R8927:Vmn2r8	UTSW	5	108802265	missense
R8928:Vmn2r8	UTSW	5	108802265	missense
R9288:Vmn2r8	UTSW	5	108802319	missense	probably benign	0.39
R9596:Vmn2r8	UTSW	5	108799330	missense	possibly damaging	0.94
R9652:Vmn2r8	UTSW	5	108803241	missense	probably benign	0.18
Z1088:Vmn2r8	UTSW	5	108801998	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- TGTGGTGGTGTTCAAAAGTAACAGTCC -3'
(R):5'- GACCAGTTTCCCTTTGTCCATCAGG -3'

Sequencing Primer
(F):5'- GTCCCATGGAAGAAATCAAGGCT -3'
(R):5'- GTCATTTCAGATGATGACCAGG -3'

Posted On

2014-04-13