Mutagenetix > Incidental Mutation

Incidental Mutation 'R1523:Vmn2r73'

167631

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r73

Ensembl Gene

ENSMUSG00000070458

Gene Name

vomeronasal 2, receptor 73

Synonyms

EG620928

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.185) question?

Stock #

R1523 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

85857547-85875938 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 85870278 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Asparagine at position 491 (Y491N)

Ref Sequence

ENSEMBL: ENSMUSP00000076687 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000077478]

AlphaFold

D3Z7M3

Predicted Effect

probably benign
Transcript: ENSMUST00000077478
AA Change: Y491N

PolyPhen 2 Score 0.019 (Sensitivity: 0.95; Specificity: 0.80)

SMART Domains

Protein: ENSMUSP00000076687
Gene: ENSMUSG00000070458
AA Change: Y491N

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	77	465	1.4e-30	PFAM
Pfam:NCD3G	508	560	5.9e-21	PFAM
Pfam:7tm_3	590	827	4.4e-51	PFAM

Coding Region Coverage

1x: 99.2%
3x: 98.3%
10x: 96.3%
20x: 92.9%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 82 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700020L24Rik	G	T	11: 83,440,406	E48*	probably null	Het
4930503B20Rik	A	G	3: 146,651,109	S15P	probably damaging	Het
4933402E13Rik	T	A	X: 62,290,906	D177E	probably benign	Het
5530400C23Rik	A	G	6: 133,294,293	E100G	possibly damaging	Het
Abcg2	T	C	6: 58,685,694	F507S	possibly damaging	Het
Adgrf5	A	T	17: 43,450,153	Q913L	probably benign	Het
Ak7	A	T	12: 105,766,608	N537I	probably benign	Het
Anks1	T	A	17: 28,051,655		probably null	Het
Arhgap32	T	A	9: 32,256,752	V677D	probably damaging	Het
Arnt	C	T	3: 95,489,654	P466L	possibly damaging	Het
Arrb1	T	G	7: 99,594,665	L274R	probably damaging	Het
Atf2	A	T	2: 73,863,208	D3E	probably damaging	Het
Baz2b	C	T	2: 59,968,637	R381Q	possibly damaging	Het
Cacna1g	C	T	11: 94,442,729		probably null	Het
Ccr10	C	T	11: 101,173,675	R343Q	probably damaging	Het
Clca2	G	T	3: 145,071,644	S822*	probably null	Het
Col12a1	A	T	9: 79,660,996	Y1649N	probably benign	Het
Col23a1	G	A	11: 51,561,916		probably null	Het
Cp	T	C	3: 19,989,065	Y1006H	probably benign	Het
Ctbs	A	G	3: 146,454,980	T101A	probably benign	Het
Cyp4a31	T	C	4: 115,569,754	F170L	probably benign	Het
Dock1	A	C	7: 134,744,247	I173L	possibly damaging	Het
Dock4	A	G	12: 40,693,025	D393G	possibly damaging	Het
Dsg1a	T	A	18: 20,322,317	S113T	probably damaging	Het
Epha3	T	C	16: 63,610,948	D530G	probably damaging	Het
Erbb4	T	A	1: 68,396,252	H162L	possibly damaging	Het
Fam131c	C	T	4: 141,382,831	T180I	probably benign	Het
Fndc1	A	G	17: 7,773,209	S552P	unknown	Het
Foxf1	A	G	8: 121,084,558		probably null	Het
Frem2	T	C	3: 53,655,407	T560A	possibly damaging	Het
Gabra4	G	A	5: 71,633,632	T289M	probably damaging	Het
Gcnt1	A	G	19: 17,329,833	V176A	probably damaging	Het
Gemin8	G	A	X: 166,180,648	S100N	probably benign	Het
Gm1527	T	C	3: 28,920,418	I460T	probably damaging	Het
Gm6729	A	G	10: 86,540,175		noncoding transcript	Het
Gprin2	T	C	14: 34,195,079	S245G	probably benign	Het
Gsdmc	A	T	15: 63,803,630	I112N	probably damaging	Het
Hspb6	A	G	7: 30,553,423	D30G	probably benign	Het
Hydin	A	G	8: 110,533,271	D2625G	probably benign	Het
Iqca	C	A	1: 90,142,731	G133V	probably null	Het
Irf2	T	A	8: 46,837,840		probably null	Het
Kdm3b	T	C	18: 34,793,173		probably null	Het
Khdc3	G	A	9: 73,103,491	E208K	possibly damaging	Het
Kifc1	A	T	17: 33,883,662	S263T	probably benign	Het
Lrig3	C	A	10: 126,008,698	T677K	probably damaging	Het
Mapkapk3	A	T	9: 107,263,623		probably null	Het
Mertk	T	C	2: 128,790,328		probably null	Het
Metrn	A	G	17: 25,794,977	*292R	probably null	Het
Mllt6	G	T	11: 97,665,023	A60S	probably damaging	Het
Mmp21	T	C	7: 133,679,045	I65M	probably benign	Het
Myo7b	A	G	18: 31,966,876	L1651P	probably damaging	Het
Nhsl1	A	G	10: 18,408,355	S15G	probably benign	Het
Nos1ap	T	C	1: 170,338,118	D192G	probably benign	Het
Nrcam	A	G	12: 44,572,249	T844A	probably damaging	Het
Pax4	A	G	6: 28,444,841	L203P	probably damaging	Het
Pbld2	T	C	10: 63,076,433	I280T	probably benign	Het
Pclo	A	G	5: 14,788,406	Y4681C	unknown	Het
Phyhip	T	A	14: 70,461,760	M1K	probably null	Het
Plppr4	T	C	3: 117,322,841	N456D	probably damaging	Het
Prpf31	T	C	7: 3,640,857	Y473H	probably damaging	Het
Rapgef2	A	T	3: 79,092,749	V564D	probably damaging	Het
Rexo1	T	C	10: 80,542,751	S1123G	probably benign	Het
Rnasel	C	A	1: 153,756,013	Q513K	probably damaging	Het
Rnf165	G	A	18: 77,462,938	T98I	probably benign	Het
Rnf213	T	C	11: 119,441,888	V2641A	probably damaging	Het
Rnf40	G	T	7: 127,590,615	R184L	probably damaging	Het
Rnf8	A	G	17: 29,626,972	K179R	probably damaging	Het
Sipa1l2	C	T	8: 125,447,613	D1309N	possibly damaging	Het
Slc25a38	T	A	9: 120,123,703	M307K	possibly damaging	Het
Snx33	T	C	9: 56,926,182	D201G	possibly damaging	Het
Sulf1	T	A	1: 12,817,350	Y249*	probably null	Het
Sult2a4	G	A	7: 13,909,860	Q261*	probably null	Het
Syndig1	G	A	2: 150,003,234	A226T	probably damaging	Het
Tcaf2	C	T	6: 42,624,451	W891*	probably null	Het
Tcf15	C	A	2: 152,143,888	T88K	probably damaging	Het
Tmem19	A	T	10: 115,347,217	M117K	probably damaging	Het
Trim32	T	C	4: 65,614,004	L266P	probably benign	Het
Vmn2r11	T	C	5: 109,053,841	I266V	probably benign	Het
Wrn	C	T	8: 33,292,716	E486K	probably benign	Het
Zfp457	T	C	13: 67,293,437	E262G	probably damaging	Het
Zfp598	A	G	17: 24,678,629	D308G	probably null	Het
Zufsp	T	C	10: 33,927,440	I549M	probably damaging	Het

Other mutations in Vmn2r73

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00158:Vmn2r73	APN	7	85857587	missense
IGL01151:Vmn2r73	APN	7	85857878	missense	probably damaging	0.99
IGL01408:Vmn2r73	APN	7	85872247	missense	probably benign	0.45
IGL01505:Vmn2r73	APN	7	85858059	nonsense	probably null
IGL01731:Vmn2r73	APN	7	85857549	makesense	probably null
IGL01818:Vmn2r73	APN	7	85869901	splice site	probably benign
IGL02236:Vmn2r73	APN	7	85872694	missense	probably benign	0.00
IGL02265:Vmn2r73	APN	7	85871639	missense	probably benign	0.02
IGL02266:Vmn2r73	APN	7	85875799	missense	possibly damaging	0.52
IGL02276:Vmn2r73	APN	7	85869772	critical splice donor site	probably null
IGL02284:Vmn2r73	APN	7	85857915	missense	possibly damaging	0.92
IGL02380:Vmn2r73	APN	7	85858175	missense	probably benign	0.00
IGL02466:Vmn2r73	APN	7	85872876	missense	probably damaging	1.00
IGL03172:Vmn2r73	APN	7	85858287	missense	probably benign	0.01
IGL03179:Vmn2r73	APN	7	85870260	missense	probably benign	0.04
G1Funyon:Vmn2r73	UTSW	7	85858302	missense	probably benign	0.00
R0077:Vmn2r73	UTSW	7	85875867	missense	probably benign	0.00
R0311:Vmn2r73	UTSW	7	85871789	missense	probably benign
R0413:Vmn2r73	UTSW	7	85871879	missense	possibly damaging	0.68
R0494:Vmn2r73	UTSW	7	85872932	missense	probably benign
R1618:Vmn2r73	UTSW	7	85875912	nonsense	probably null
R1667:Vmn2r73	UTSW	7	85857681	missense	probably benign	0.00
R1728:Vmn2r73	UTSW	7	85857878	missense	probably damaging	1.00
R1729:Vmn2r73	UTSW	7	85857878	missense	probably damaging	1.00
R1747:Vmn2r73	UTSW	7	85858167	missense	probably damaging	1.00
R1784:Vmn2r73	UTSW	7	85857878	missense	probably damaging	1.00
R2152:Vmn2r73	UTSW	7	85857728	missense	probably benign	0.03
R2395:Vmn2r73	UTSW	7	85857767	missense	probably damaging	1.00
R2415:Vmn2r73	UTSW	7	85872223	missense	probably damaging	1.00
R2926:Vmn2r73	UTSW	7	85871663	missense	probably benign	0.17
R3719:Vmn2r73	UTSW	7	85870374	missense	probably damaging	1.00
R3766:Vmn2r73	UTSW	7	85871990	missense	probably benign
R3838:Vmn2r73	UTSW	7	85858050	missense	probably benign	0.00
R3890:Vmn2r73	UTSW	7	85857936	missense	probably benign
R4030:Vmn2r73	UTSW	7	85871836	missense	possibly damaging	0.92
R4247:Vmn2r73	UTSW	7	85872675	missense	probably damaging	0.99
R4409:Vmn2r73	UTSW	7	85871560	missense	probably damaging	0.97
R4427:Vmn2r73	UTSW	7	85857773	missense	probably damaging	1.00
R4430:Vmn2r73	UTSW	7	85870241	missense	probably benign
R4552:Vmn2r73	UTSW	7	85875847	missense	probably benign	0.12
R4779:Vmn2r73	UTSW	7	85871715	nonsense	probably null
R4906:Vmn2r73	UTSW	7	85857621	missense	probably damaging	0.99
R4942:Vmn2r73	UTSW	7	85870374	missense	probably damaging	1.00
R5280:Vmn2r73	UTSW	7	85857947	missense	probably damaging	0.98
R5344:Vmn2r73	UTSW	7	85875838	missense	probably benign	0.00
R5478:Vmn2r73	UTSW	7	85869788	missense	probably damaging	1.00
R5527:Vmn2r73	UTSW	7	85858367	missense	possibly damaging	0.65
R5691:Vmn2r73	UTSW	7	85858091	missense	probably damaging	1.00
R5826:Vmn2r73	UTSW	7	85875748	missense	possibly damaging	0.77
R6018:Vmn2r73	UTSW	7	85872667	missense	possibly damaging	0.48
R6082:Vmn2r73	UTSW	7	85858221	missense	probably benign	0.00
R6111:Vmn2r73	UTSW	7	85871789	missense	probably benign
R6233:Vmn2r73	UTSW	7	85869891	missense	probably benign	0.00
R6278:Vmn2r73	UTSW	7	85872932	missense	probably benign
R6283:Vmn2r73	UTSW	7	85871841	missense	probably benign	0.00
R6307:Vmn2r73	UTSW	7	85857620	missense	probably damaging	1.00
R6413:Vmn2r73	UTSW	7	85870336	nonsense	probably null
R6782:Vmn2r73	UTSW	7	85870355	missense	probably benign	0.00
R6884:Vmn2r73	UTSW	7	85858005	missense	probably benign	0.05
R6921:Vmn2r73	UTSW	7	85858238	missense	probably benign	0.27
R7169:Vmn2r73	UTSW	7	85858455	nonsense	probably null
R7206:Vmn2r73	UTSW	7	85872867	missense	probably benign	0.03
R7311:Vmn2r73	UTSW	7	85871984	missense	possibly damaging	0.80
R7650:Vmn2r73	UTSW	7	85871939	missense	probably benign
R7811:Vmn2r73	UTSW	7	85875748	missense	possibly damaging	0.77
R8263:Vmn2r73	UTSW	7	85858411	missense	probably benign
R8301:Vmn2r73	UTSW	7	85858302	missense	probably benign	0.00
R8341:Vmn2r73	UTSW	7	85857920	missense	probably benign	0.01
R8500:Vmn2r73	UTSW	7	85870430	missense	probably benign	0.01
R8673:Vmn2r73	UTSW	7	85872694	missense	probably benign	0.01
R8719:Vmn2r73	UTSW	7	85871887	missense	probably damaging	0.96
R8783:Vmn2r73	UTSW	7	85858460	missense	probably damaging	0.96
R8828:Vmn2r73	UTSW	7	85871971	missense	probably damaging	0.98
R8860:Vmn2r73	UTSW	7	85872941	splice site	probably benign
R9002:Vmn2r73	UTSW	7	85858076	missense	probably benign	0.32
R9008:Vmn2r73	UTSW	7	85872696	missense	probably damaging	0.96
R9159:Vmn2r73	UTSW	7	85872723	missense	possibly damaging	0.90
R9180:Vmn2r73	UTSW	7	85857915	missense	probably benign	0.07
R9198:Vmn2r73	UTSW	7	85869779	missense	probably benign	0.01
R9329:Vmn2r73	UTSW	7	85869865	missense	probably benign
R9448:Vmn2r73	UTSW	7	85872819	missense	probably benign	0.00
R9463:Vmn2r73	UTSW	7	85857587	missense
R9589:Vmn2r73	UTSW	7	85870451	missense	probably benign	0.03
X0023:Vmn2r73	UTSW	7	85858456	missense	probably benign	0.31
Z1176:Vmn2r73	UTSW	7	85871968	missense	probably damaging	0.99
Z1177:Vmn2r73	UTSW	7	85872272	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TCCTGGTCTGCAAGGCATACTACATA -3'
(R):5'- TCCTGCTGGAGACCTAGTTAACATGAA -3'

Sequencing Primer
(F):5'- CTGCAAGGCATACTACATATTGAGG -3'
(R):5'- TAACATGAACCAGAATTTGAAACAGG -3'

Posted On

2014-04-13