Mutagenetix > Incidental Mutation

Incidental Mutation 'R1525:Zfp382'

167781

Institutional Source

Beutler Lab

Gene Symbol

Zfp382

Ensembl Gene

ENSMUSG00000074220

Gene Name

zinc finger protein 382

Synonyms

5930415A09Rik

MMRRC Submission

040872-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.132) question?

Stock #

R1525 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

29821367-29834375 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 29833144 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glycine to Glutamic Acid at position 265 (G265E)

Ref Sequence

ENSEMBL: ENSMUSP00000096196 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000098596] [ENSMUST00000153792]

AlphaFold

B2RXC5

Predicted Effect

probably damaging
Transcript: ENSMUST00000098596
AA Change: G265E

PolyPhen 2 Score 0.991 (Sensitivity: 0.71; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000096196
Gene: ENSMUSG00000074220
AA Change: G265E

Domain	Start	End	E-Value	Type
low complexity region	2	15	N/A	INTRINSIC
KRAB	42	102	3.36e-39	SMART
ZnF_C2H2	325	347	1.84e-4	SMART
ZnF_C2H2	353	375	7.26e-3	SMART
ZnF_C2H2	381	403	2.71e-2	SMART
ZnF_C2H2	409	431	4.47e-3	SMART
ZnF_C2H2	437	459	2.12e-4	SMART
ZnF_C2H2	465	487	3.95e-4	SMART
ZnF_C2H2	493	515	1.12e-3	SMART
ZnF_C2H2	521	543	2.57e-3	SMART
ZnF_C2H2	549	571	3.63e-3	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000153792

SMART Domains

Protein: ENSMUSP00000123508
Gene: ENSMUSG00000074220

Domain	Start	End	E-Value	Type
low complexity region	2	15	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000156543

Coding Region Coverage

1x: 99.1%
3x: 98.3%
10x: 96.2%
20x: 92.6%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a KRAB domain zinc finger transcription factor (KZNF). KZNFs play critical roles in the regulation of many cellular processes including differentiation, proliferation and apoptosis. The encoded protein inhibits activating protein 1 (AP-1) and nuclear factor kappa-B (NF-kB) signaling and may function as a tumor suppressor in multiple carcinomas. This gene is found in a cluster with other zinc finger protein genes on the long arm of chromosome 19, and alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Feb 2012]

Allele List at MGI

Other mutations in this stock

Total: 52 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcc3	A	T	11: 94,252,062 (GRCm39)	H840Q	probably benign	Het
Amotl2	C	T	9: 102,605,767 (GRCm39)	R540C	probably damaging	Het
Brpf1	A	G	6: 113,294,115 (GRCm39)	E605G	probably damaging	Het
Cacna2d3	T	C	14: 28,694,199 (GRCm39)	I865V	probably benign	Het
Cdh24	A	T	14: 54,876,046 (GRCm39)	F199I	probably damaging	Het
Cdk9	A	G	2: 32,600,521 (GRCm39)	V27A	probably damaging	Het
Cfap69	G	T	5: 5,690,230 (GRCm39)		probably null	Het
Cyp2d11	G	T	15: 82,273,498 (GRCm39)	L458I	probably damaging	Het
Dchs1	T	C	7: 105,408,138 (GRCm39)	E1898G	probably damaging	Het
Dennd4b	G	T	3: 90,178,177 (GRCm39)	L456F	probably damaging	Het
Dgat1	T	C	15: 76,395,786 (GRCm39)	T66A	probably benign	Het
Dock10	C	A	1: 80,583,881 (GRCm39)		probably null	Het
Fam110b	A	G	4: 5,799,578 (GRCm39)	D332G	possibly damaging	Het
Fem1al	A	C	11: 29,773,994 (GRCm39)	Y488D	probably benign	Het
Frmd4b	G	A	6: 97,273,347 (GRCm39)	P628S	probably damaging	Het
Ice1	A	T	13: 70,753,529 (GRCm39)	H852Q	probably benign	Het
Il17ra	T	C	6: 120,450,751 (GRCm39)	V116A	probably damaging	Het
Ints9	T	C	14: 65,232,460 (GRCm39)	I173T	probably benign	Het
Kctd14	A	T	7: 97,107,074 (GRCm39)	M110L	probably benign	Het
Krt6a	T	G	15: 101,602,637 (GRCm39)	Y16S	probably benign	Het
Lamc2	T	C	1: 153,006,502 (GRCm39)	N883S	probably benign	Het
Larp4b	C	T	13: 9,195,486 (GRCm39)	T195M	probably damaging	Het
Lrp1	A	G	10: 127,375,398 (GRCm39)	L4432P	probably damaging	Het
Mei4	T	C	9: 81,772,252 (GRCm39)	S22P	probably damaging	Het
Mep1a	T	C	17: 43,802,527 (GRCm39)	Q166R	probably damaging	Het
Mroh2b	C	A	15: 4,980,612 (GRCm39)		probably null	Het
Myoc	T	G	1: 162,476,220 (GRCm39)	L308R	probably damaging	Het
Ndn	C	T	7: 61,998,256 (GRCm39)	P34L	probably benign	Het
Or1j21	A	T	2: 36,684,155 (GRCm39)	R302S	probably null	Het
Or2t6	T	A	14: 14,175,725 (GRCm38)	Y119F	probably damaging	Het
Or4c101	C	G	2: 88,389,985 (GRCm39)	S57R	probably damaging	Het
Pdilt	T	A	7: 119,087,217 (GRCm39)	T478S	probably damaging	Het
Pias1	T	C	9: 62,827,769 (GRCm39)	K222E	probably damaging	Het
Prss16	A	C	13: 22,193,613 (GRCm39)	L61V	possibly damaging	Het
Pttg1ip2	C	A	5: 5,502,019 (GRCm39)	W144C	probably benign	Het
Pvr	G	A	7: 19,644,551 (GRCm39)	Q328*	probably null	Het
Ranbp3	A	G	17: 57,017,865 (GRCm39)	D481G	possibly damaging	Het
Rsf1	CGGCGGCGG	CGGCGGCGGGGGCGGCGG	7: 97,229,115 (GRCm39)		probably benign	Het
Ryr3	G	T	2: 112,508,435 (GRCm39)	D3419E	probably damaging	Het
Scn1a	C	T	2: 66,149,806 (GRCm39)	W946*	probably null	Het
Sh3pxd2a	T	C	19: 47,266,864 (GRCm39)	K242E	probably damaging	Het
Slc34a2	A	G	5: 53,226,848 (GRCm39)	D657G	probably benign	Het
Stard9	T	A	2: 120,532,533 (GRCm39)	I2930K	probably benign	Het
Syna	T	C	5: 134,588,112 (GRCm39)	D279G	probably benign	Het
Tfr2	T	C	5: 137,577,292 (GRCm39)	F415L	probably benign	Het
Tmem97	T	A	11: 78,433,586 (GRCm39)	Y103F	probably damaging	Het
Tmem97	A	T	11: 78,433,587 (GRCm39)	Y103N	probably damaging	Het
Txndc2	T	A	17: 65,945,310 (GRCm39)	D289V	probably damaging	Het
Zbtb1	T	G	12: 76,433,206 (GRCm39)	D397E	probably benign	Het
Zc3h18	T	C	8: 123,140,677 (GRCm39)	S847P	probably benign	Het
Zfp410	T	C	12: 84,369,740 (GRCm39)	L39S	probably damaging	Het
Zfp729a	G	T	13: 67,767,440 (GRCm39)	P930T	probably benign	Het

Other mutations in Zfp382

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02724:Zfp382	APN	7	29,833,162 (GRCm39)	missense	probably benign	0.00
IGL03116:Zfp382	APN	7	29,833,614 (GRCm39)	missense	probably damaging	1.00
R1051:Zfp382	UTSW	7	29,833,435 (GRCm39)	missense	probably damaging	1.00
R1371:Zfp382	UTSW	7	29,833,114 (GRCm39)	missense	probably benign	0.36
R1513:Zfp382	UTSW	7	29,832,721 (GRCm39)	missense	probably benign	0.04
R2416:Zfp382	UTSW	7	29,833,828 (GRCm39)	missense	probably damaging	1.00
R2432:Zfp382	UTSW	7	29,833,174 (GRCm39)	missense	probably benign
R4864:Zfp382	UTSW	7	29,832,885 (GRCm39)	missense	possibly damaging	0.58
R4956:Zfp382	UTSW	7	29,830,979 (GRCm39)	missense	probably benign	0.00
R5734:Zfp382	UTSW	7	29,833,855 (GRCm39)	missense	probably damaging	1.00
R5796:Zfp382	UTSW	7	29,832,774 (GRCm39)	missense	probably damaging	1.00
R6062:Zfp382	UTSW	7	29,833,015 (GRCm39)	missense	probably damaging	1.00
R6300:Zfp382	UTSW	7	29,831,054 (GRCm39)	splice site	probably null
R6312:Zfp382	UTSW	7	29,833,963 (GRCm39)	missense	probably damaging	0.99
R6894:Zfp382	UTSW	7	29,825,261 (GRCm39)	missense	probably benign
R7764:Zfp382	UTSW	7	29,832,700 (GRCm39)	missense	probably benign	0.04
R7771:Zfp382	UTSW	7	29,832,760 (GRCm39)	missense	probably damaging	1.00
R7794:Zfp382	UTSW	7	29,831,035 (GRCm39)	missense	possibly damaging	0.84
R8207:Zfp382	UTSW	7	29,833,840 (GRCm39)	missense	possibly damaging	0.74
R8267:Zfp382	UTSW	7	29,833,929 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TGGGAGGGATCAATCCTGACTTCG -3'
(R):5'- AAAGCTGTTCCCACAGTAAGGACAC -3'

Sequencing Primer
(F):5'- GCAGGAGAAAACGCATCCC -3'
(R):5'- ACACTGGAAGGGCCTCTC -3'

Posted On

2014-04-13