Mutagenetix > Incidental Mutation

Incidental Mutation 'R1498:Lyst'

169065

Institutional Source

Beutler Lab

Gene Symbol

Lyst

Ensembl Gene

ENSMUSG00000019726

Gene Name

lysosomal trafficking regulator

Synonyms

D13Sfk13

MMRRC Submission

039549-MU

Accession Numbers

Ncbi RefSeq: NM_010748.2; MGI:107448

Essential gene?

Possibly non essential (E-score: 0.298) question?

Stock #

R1498 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

13590397-13778803 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 13650375 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Lysine at position 1525 (I1525K)

Ref Sequence

ENSEMBL: ENSMUSP00000106188 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000110559]

AlphaFold

no structure available at present

Predicted Effect

possibly damaging
Transcript: ENSMUST00000110559
AA Change: I1525K

PolyPhen 2 Score 0.485 (Sensitivity: 0.88; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000106188
Gene: ENSMUSG00000019726
AA Change: I1525K

Domain	Start	End	E-Value	Type
low complexity region	26	36	N/A	INTRINSIC
low complexity region	72	82	N/A	INTRINSIC
low complexity region	399	412	N/A	INTRINSIC
low complexity region	1333	1344	N/A	INTRINSIC
low complexity region	2295	2307	N/A	INTRINSIC
low complexity region	2427	2445	N/A	INTRINSIC
low complexity region	2534	2546	N/A	INTRINSIC
Pfam:PH_BEACH	3006	3101	5.8e-25	PFAM
Beach	3118	3408	1.25e-193	SMART
Blast:Beach	3441	3478	9e-13	BLAST
WD40	3539	3579	5.75e-1	SMART
WD40	3591	3630	2.89e-5	SMART
WD40	3633	3676	1.38e0	SMART
WD40	3724	3765	1.27e-1	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000220937

Meta Mutation Damage Score

0.0982

Coding Region Coverage

1x: 99.1%
3x: 98.3%
10x: 96.2%
20x: 92.6%

Validation Efficiency

97% (83/86)

MGI Phenotype

Strain: 1855968
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a protein that regulates intracellular protein trafficking in endosomes, and may be involved in pigmentation. Mutations in this gene are associated with Chediak-Higashi syndrome, a lysosomal storage disorder. Alternative splicing results in multiple transcript variants, though the full-length nature of some of these variants has not been determined. [provided by RefSeq, Apr 2013]
PHENOTYPE: Homozygous mice have a phenotype similar to human Chediak-Higashi syndrome patients, exhibiting lysosomal dysfunction with resultant protein storage; diluted coat color; abnormal melanogenesis; immune cell dysfunction resulting in increased susceptibility to bacterial, viral, and parasitic infections and decreased cytotoxic activity against tumor cells. [provided by MGI curators]

Allele List at MGI

All alleles(52) : Targeted(3) Gene trapped(34) Spontaneous(8) Chemically induced(6) Radiation induced(1)

Other mutations in this stock

Total: 84 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1600015I10Rik	A	G	6: 48,931,371 (GRCm38)	H435R	probably benign	Het
Acly	A	G	11: 100,483,801 (GRCm38)	S799P	probably benign	Het
Acvr1b	T	C	15: 101,194,010 (GRCm38)	F57S	probably benign	Het
Adamts5	T	A	16: 85,900,102 (GRCm38)	T56S	possibly damaging	Het
Adrb2	T	C	18: 62,178,933 (GRCm38)	T274A	probably damaging	Het
Agr3	A	T	12: 35,934,380 (GRCm38)		probably null	Het
Ankrd6	A	G	4: 32,810,289 (GRCm38)	M405T	probably benign	Het
Aqp5	T	C	15: 99,593,247 (GRCm38)	I60T	probably damaging	Het
Arf5	G	T	6: 28,426,154 (GRCm38)	R180L	probably benign	Het
Asap3	A	G	4: 136,239,194 (GRCm38)	N528D	probably benign	Het
Atmin	G	A	8: 116,954,801 (GRCm38)	E180K	probably benign	Het
Bcl11a	A	T	11: 24,164,005 (GRCm38)	E449D	probably damaging	Het
Cep128	A	T	12: 91,366,417 (GRCm38)	F25I	probably benign	Het
Cfp	GCAC	GC	X: 20,929,666 (GRCm38)		probably null	Het
Chpt1	T	A	10: 88,477,104 (GRCm38)	Y282F	possibly damaging	Het
Cul7	A	G	17: 46,655,710 (GRCm38)	D355G	probably benign	Het
Daxx	T	C	17: 33,912,253 (GRCm38)	Y385H	probably damaging	Het
Dnah2	A	C	11: 69,520,667 (GRCm38)		probably null	Het
Dscc1	CTGAATGAAT	CTGAAT	15: 55,080,176 (GRCm38)		probably benign	Het
Erc2	T	A	14: 28,302,898 (GRCm38)	M578K	probably benign	Het
Evc	T	A	5: 37,323,700 (GRCm38)	H267L	possibly damaging	Het
Exosc10	A	C	4: 148,581,786 (GRCm38)	Q837P	possibly damaging	Het
Fasn	G	A	11: 120,815,419 (GRCm38)	T1033I	probably damaging	Het
Fat1	C	A	8: 45,025,484 (GRCm38)	Y2522*	probably null	Het
Fbxw8	G	A	5: 118,065,785 (GRCm38)		probably benign	Het
Fyn	A	G	10: 39,532,124 (GRCm38)	D321G	possibly damaging	Het
Gm14685	G	T	X: 73,127,655 (GRCm38)	G218C	probably damaging	Het
Gm156	A	G	6: 129,771,740 (GRCm38)	S105P	probably damaging	Het
Grm6	G	A	11: 50,857,256 (GRCm38)	V398M	probably damaging	Het
Gtpbp1	T	A	15: 79,719,021 (GRCm38)		probably null	Het
Hnf1a	A	G	5: 114,970,537 (GRCm38)	V103A	probably damaging	Het
Hsp90aa1	A	T	12: 110,695,688 (GRCm38)		probably null	Het
Iqgap2	C	T	13: 95,646,805 (GRCm38)	V1288I	probably benign	Het
Irx5	A	G	8: 92,359,886 (GRCm38)	D199G	probably damaging	Het
Krt9	G	T	11: 100,188,369 (GRCm38)	C732*	probably null	Het
Lifr	T	C	15: 7,190,618 (GRCm38)	S910P	probably damaging	Het
Lrig1	G	A	6: 94,627,987 (GRCm38)	A209V	possibly damaging	Het
Lrrk1	T	A	7: 66,302,671 (GRCm38)	R506*	probably null	Het
Mad2l1	C	T	6: 66,539,842 (GRCm38)	Q173*	probably null	Het
Mcoln1	T	A	8: 3,512,861 (GRCm38)	I524N	probably damaging	Het
Mmp3	A	T	9: 7,446,967 (GRCm38)	D49V	possibly damaging	Het
Morc3	A	T	16: 93,853,855 (GRCm38)	N321I	probably damaging	Het
Mrpl48	T	C	7: 100,546,488 (GRCm38)		probably benign	Het
Myo6	T	A	9: 80,307,679 (GRCm38)	D1231E	probably damaging	Het
Nlrc4	A	G	17: 74,446,413 (GRCm38)	I325T	probably benign	Het
Olfr1079	A	G	2: 86,538,558 (GRCm38)	V117A	probably benign	Het
Olfr1314	A	T	2: 112,092,593 (GRCm38)	M36K	probably benign	Het
Olfr348	A	T	2: 36,787,346 (GRCm38)	I274F	probably damaging	Het
Olfr699	T	C	7: 106,790,416 (GRCm38)	Y195C	possibly damaging	Het
Olfr894	C	G	9: 38,219,380 (GRCm38)	P183A	probably damaging	Het
Parvg	T	A	15: 84,334,631 (GRCm38)	S230T	possibly damaging	Het
Pcca	T	C	14: 122,616,818 (GRCm38)	I118T	probably damaging	Het
Pde6a	A	G	18: 61,232,860 (GRCm38)	N219S	possibly damaging	Het
Phldb1	G	T	9: 44,701,618 (GRCm38)	P935Q	possibly damaging	Het
Plppr5	T	C	3: 117,662,612 (GRCm38)	S261P	probably damaging	Het
Ppl	T	C	16: 5,104,765 (GRCm38)	N260S	probably benign	Het
Ppp1r3e	A	T	14: 54,876,425 (GRCm38)	H263Q	probably benign	Het
Ppp3cb	T	C	14: 20,509,499 (GRCm38)		probably null	Het
Ppt2	A	T	17: 34,623,101 (GRCm38)	D185E	probably benign	Het
Prr27	A	T	5: 87,850,741 (GRCm38)		probably benign	Het
Ptcd3	A	T	6: 71,893,495 (GRCm38)	V327E	probably damaging	Het
Rsf1	GCG	GCGACG	7: 97,579,907 (GRCm38)		probably benign	Het
Rtp1	A	T	16: 23,431,220 (GRCm38)	M112L	probably benign	Het
Slc22a5	A	T	11: 53,869,314 (GRCm38)	L392Q	probably damaging	Het
Slc6a7	A	G	18: 60,996,692 (GRCm38)	I614T	probably benign	Het
Slu7	C	A	11: 43,438,217 (GRCm38)	P89T	possibly damaging	Het
Smarcc2	T	C	10: 128,482,192 (GRCm38)	V618A	probably benign	Het
Sorl1	C	T	9: 42,041,073 (GRCm38)	R729Q	probably damaging	Het
Sptbn2	T	C	19: 4,744,246 (GRCm38)	F1493L	possibly damaging	Het
Sulf1	C	T	1: 12,848,350 (GRCm38)	T66I	probably damaging	Het
Tgtp2	A	G	11: 49,059,338 (GRCm38)	Y136H	probably damaging	Het
Tmem117	T	C	15: 94,638,361 (GRCm38)	F92S	probably damaging	Het
Tmprss9	T	A	10: 80,895,100 (GRCm38)	V820E	probably benign	Het
Tpcn2	A	G	7: 145,268,911 (GRCm38)	Y266H	probably damaging	Het
Trat1	A	G	16: 48,734,941 (GRCm38)	S143P	probably benign	Het
Vmn1r49	T	A	6: 90,072,316 (GRCm38)	R235W	probably damaging	Het
Vmn1r71	A	T	7: 10,748,648 (GRCm38)	C38S	probably benign	Het
Vmn2r6	T	A	3: 64,556,469 (GRCm38)	T315S	probably damaging	Het
Wdr36	A	G	18: 32,852,968 (GRCm38)	D575G	possibly damaging	Het
Zfp292	G	T	4: 34,805,397 (GRCm38)	A2549E	possibly damaging	Het
Zfp354a	A	T	11: 51,070,246 (GRCm38)	H426L	probably damaging	Het
Zfp407	C	T	18: 84,559,773 (GRCm38)	A1072T	probably benign	Het
Zfp648	T	A	1: 154,205,373 (GRCm38)	I426N	probably damaging	Het
Zfp72	T	C	13: 74,372,617 (GRCm38)	N114S	probably benign	Het

Other mutations in Lyst

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00156:Lyst	APN	13	13,648,878 (GRCm38)	missense	probably benign
IGL00474:Lyst	APN	13	13,643,536 (GRCm38)	missense	possibly damaging	0.48
IGL00484:Lyst	APN	13	13,709,603 (GRCm38)	missense	probably benign	0.02
IGL00492:Lyst	APN	13	13,678,175 (GRCm38)	missense	possibly damaging	0.54
IGL00807:Lyst	APN	13	13,650,423 (GRCm38)	missense	possibly damaging	0.91
IGL00949:Lyst	APN	13	13,635,485 (GRCm38)	missense	possibly damaging	0.87
IGL00952:Lyst	APN	13	13,678,107 (GRCm38)	missense	probably benign	0.05
IGL01305:Lyst	APN	13	13,678,056 (GRCm38)	missense	probably benign	0.01
IGL01317:Lyst	APN	13	13,670,870 (GRCm38)	missense	probably benign
IGL01419:Lyst	APN	13	13,635,838 (GRCm38)	missense	probably benign	0.00
IGL01445:Lyst	APN	13	13,651,714 (GRCm38)	missense	probably benign	0.00
IGL01690:Lyst	APN	13	13,743,246 (GRCm38)	missense	probably damaging	1.00
IGL01791:Lyst	APN	13	13,635,302 (GRCm38)	missense	probably damaging	1.00
IGL01809:Lyst	APN	13	13,637,803 (GRCm38)	missense	probably damaging	1.00
IGL01896:Lyst	APN	13	13,635,577 (GRCm38)	missense	probably benign	0.04
IGL01938:Lyst	APN	13	13,637,424 (GRCm38)	missense	possibly damaging	0.93
IGL01986:Lyst	APN	13	13,775,627 (GRCm38)	critical splice donor site	probably null
IGL02022:Lyst	APN	13	13,664,044 (GRCm38)	nonsense	probably null
IGL02044:Lyst	APN	13	13,712,846 (GRCm38)	missense	probably damaging	1.00
IGL02157:Lyst	APN	13	13,660,956 (GRCm38)	missense	probably benign
IGL02185:Lyst	APN	13	13,661,093 (GRCm38)	nonsense	probably null
IGL02215:Lyst	APN	13	13,660,956 (GRCm38)	missense	probably benign
IGL02245:Lyst	APN	13	13,660,956 (GRCm38)	missense	probably benign
IGL02246:Lyst	APN	13	13,660,956 (GRCm38)	missense	probably benign
IGL02247:Lyst	APN	13	13,660,956 (GRCm38)	missense	probably benign
IGL02297:Lyst	APN	13	13,638,092 (GRCm38)	nonsense	probably null
IGL02411:Lyst	APN	13	13,660,956 (GRCm38)	missense	probably benign
IGL02415:Lyst	APN	13	13,660,956 (GRCm38)	missense	probably benign
IGL02419:Lyst	APN	13	13,660,956 (GRCm38)	missense	probably benign
IGL02420:Lyst	APN	13	13,660,956 (GRCm38)	missense	probably benign
IGL02429:Lyst	APN	13	13,660,956 (GRCm38)	missense	probably benign
IGL02501:Lyst	APN	13	13,711,645 (GRCm38)	missense	probably benign	0.02
IGL02522:Lyst	APN	13	13,634,705 (GRCm38)	missense	possibly damaging	0.81
IGL02535:Lyst	APN	13	13,650,342 (GRCm38)	missense	probably benign	0.00
IGL02596:Lyst	APN	13	13,660,956 (GRCm38)	missense	probably benign
IGL02601:Lyst	APN	13	13,660,956 (GRCm38)	missense	probably benign
IGL02603:Lyst	APN	13	13,660,956 (GRCm38)	missense	probably benign
IGL02608:Lyst	APN	13	13,712,754 (GRCm38)	missense	probably damaging	0.98
IGL02622:Lyst	APN	13	13,681,390 (GRCm38)	missense	probably damaging	1.00
IGL02690:Lyst	APN	13	13,641,125 (GRCm38)	missense	possibly damaging	0.58
IGL02715:Lyst	APN	13	13,674,320 (GRCm38)	splice site	probably null
IGL02725:Lyst	APN	13	13,760,827 (GRCm38)	missense	probably damaging	1.00
IGL02729:Lyst	APN	13	13,746,609 (GRCm38)	missense	possibly damaging	0.95
IGL02729:Lyst	APN	13	13,674,339 (GRCm38)	missense	possibly damaging	0.81
IGL02820:Lyst	APN	13	13,638,058 (GRCm38)	missense	probably benign	0.03
IGL02945:Lyst	APN	13	13,761,198 (GRCm38)	missense	possibly damaging	0.48
IGL02981:Lyst	APN	13	13,634,911 (GRCm38)	missense	probably damaging	0.99
IGL03087:Lyst	APN	13	13,635,056 (GRCm38)	missense	probably damaging	1.00
IGL03149:Lyst	APN	13	13,681,444 (GRCm38)	missense	probably benign	0.14
IGL03158:Lyst	APN	13	13,651,752 (GRCm38)	critical splice donor site	probably null
IGL03226:Lyst	APN	13	13,709,559 (GRCm38)	missense	probably benign	0.01
IGL03242:Lyst	APN	13	13,656,881 (GRCm38)	nonsense	probably null
IGL03385:Lyst	APN	13	13,656,980 (GRCm38)	nonsense	probably null
50-cal	UTSW	13	13,708,212 (GRCm38)	critical splice donor site	probably null
charcoal	UTSW	13	13,696,761 (GRCm38)	nonsense	probably null
charlotte_gray	UTSW	13	13,602,026 (GRCm38)	intron	probably benign
charzard	UTSW	13	13,647,083 (GRCm38)	nonsense	probably null
grey_wolf	UTSW	13	0 ()	unclassified
lightspeed	UTSW	13	13,740,536 (GRCm38)	missense	possibly damaging	0.91
pardon	UTSW	13	13,677,952 (GRCm38)	missense	probably benign	0.00
robin	UTSW	13	13,648,802 (GRCm38)	nonsense	probably null
sooty	UTSW	13	0 ()	unclassified
souris	UTSW	13	13,683,223 (GRCm38)	unclassified	probably benign
Swallow	UTSW	13	13,757,422 (GRCm38)	missense	probably benign	0.00
vulpix	UTSW	13	13,696,794 (GRCm38)	splice site	probably null
ANU22:Lyst	UTSW	13	13,678,056 (GRCm38)	missense	probably benign	0.01
IGL02835:Lyst	UTSW	13	13,661,100 (GRCm38)	missense	possibly damaging	0.82
P0031:Lyst	UTSW	13	13,664,031 (GRCm38)	missense	probably damaging	1.00
R0012:Lyst	UTSW	13	13,687,694 (GRCm38)	missense	probably benign	0.10
R0012:Lyst	UTSW	13	13,687,694 (GRCm38)	missense	probably benign	0.10
R0031:Lyst	UTSW	13	13,708,156 (GRCm38)	missense	probably benign	0.14
R0115:Lyst	UTSW	13	13,677,952 (GRCm38)	missense	probably benign	0.00
R0212:Lyst	UTSW	13	13,635,985 (GRCm38)	missense	possibly damaging	0.93
R0386:Lyst	UTSW	13	13,708,214 (GRCm38)	splice site	probably benign
R0393:Lyst	UTSW	13	13,647,079 (GRCm38)	missense	probably benign	0.01
R0415:Lyst	UTSW	13	13,711,610 (GRCm38)	splice site	probably benign
R0446:Lyst	UTSW	13	13,638,048 (GRCm38)	missense	probably benign	0.00
R0481:Lyst	UTSW	13	13,677,952 (GRCm38)	missense	probably benign	0.00
R0499:Lyst	UTSW	13	13,616,713 (GRCm38)	missense	probably damaging	1.00
R0506:Lyst	UTSW	13	13,638,015 (GRCm38)	missense	probably benign
R0530:Lyst	UTSW	13	13,757,306 (GRCm38)	splice site	probably benign
R0541:Lyst	UTSW	13	13,681,293 (GRCm38)	missense	probably benign	0.00
R0570:Lyst	UTSW	13	13,709,386 (GRCm38)	missense	probably benign	0.26
R0680:Lyst	UTSW	13	13,650,341 (GRCm38)	missense	probably benign	0.01
R0842:Lyst	UTSW	13	13,678,241 (GRCm38)	nonsense	probably null
R0848:Lyst	UTSW	13	13,634,930 (GRCm38)	missense	probably benign	0.00
R1014:Lyst	UTSW	13	13,634,060 (GRCm38)	missense	possibly damaging	0.49
R1205:Lyst	UTSW	13	13,680,202 (GRCm38)	missense	probably benign
R1251:Lyst	UTSW	13	13,634,483 (GRCm38)	missense	probably benign	0.00
R1304:Lyst	UTSW	13	13,751,984 (GRCm38)	nonsense	probably null
R1398:Lyst	UTSW	13	13,740,536 (GRCm38)	missense	possibly damaging	0.91
R1445:Lyst	UTSW	13	13,640,054 (GRCm38)	missense	possibly damaging	0.94
R1475:Lyst	UTSW	13	13,708,212 (GRCm38)	critical splice donor site	probably null
R1479:Lyst	UTSW	13	13,634,482 (GRCm38)	missense	probably benign	0.00
R1484:Lyst	UTSW	13	13,678,190 (GRCm38)	missense	probably benign	0.01
R1540:Lyst	UTSW	13	13,635,101 (GRCm38)	missense	possibly damaging	0.81
R1611:Lyst	UTSW	13	13,634,897 (GRCm38)	missense	probably damaging	0.97
R1653:Lyst	UTSW	13	13,635,226 (GRCm38)	missense	probably damaging	1.00
R1669:Lyst	UTSW	13	13,644,087 (GRCm38)	missense	possibly damaging	0.90
R1686:Lyst	UTSW	13	13,634,705 (GRCm38)	missense	possibly damaging	0.81
R1694:Lyst	UTSW	13	13,661,161 (GRCm38)	missense	probably damaging	0.98
R1747:Lyst	UTSW	13	13,757,422 (GRCm38)	missense	probably benign	0.00
R1793:Lyst	UTSW	13	13,647,083 (GRCm38)	nonsense	probably null
R1871:Lyst	UTSW	13	13,651,712 (GRCm38)	missense	probably benign	0.00
R1905:Lyst	UTSW	13	13,634,134 (GRCm38)	missense	probably benign
R1958:Lyst	UTSW	13	13,616,618 (GRCm38)	missense	probably damaging	1.00
R1969:Lyst	UTSW	13	13,730,344 (GRCm38)	missense	probably damaging	0.99
R2040:Lyst	UTSW	13	13,641,222 (GRCm38)	missense	probably benign	0.00
R2109:Lyst	UTSW	13	13,712,820 (GRCm38)	missense	possibly damaging	0.46
R2116:Lyst	UTSW	13	13,635,701 (GRCm38)	missense	probably damaging	0.99
R2121:Lyst	UTSW	13	13,660,971 (GRCm38)	missense	probably damaging	1.00
R2127:Lyst	UTSW	13	13,635,262 (GRCm38)	missense	probably damaging	1.00
R2187:Lyst	UTSW	13	13,709,341 (GRCm38)	missense	possibly damaging	0.61
R2238:Lyst	UTSW	13	13,743,263 (GRCm38)	missense	probably benign	0.41
R2258:Lyst	UTSW	13	13,637,658 (GRCm38)	missense	probably benign	0.00
R2292:Lyst	UTSW	13	13,740,495 (GRCm38)	missense	probably damaging	1.00
R2368:Lyst	UTSW	13	13,696,663 (GRCm38)	missense	probably damaging	0.96
R2908:Lyst	UTSW	13	13,669,873 (GRCm38)	missense	probably benign	0.03
R3001:Lyst	UTSW	13	13,696,705 (GRCm38)	missense	probably benign
R3002:Lyst	UTSW	13	13,696,705 (GRCm38)	missense	probably benign
R3024:Lyst	UTSW	13	13,658,687 (GRCm38)	missense	probably benign
R3113:Lyst	UTSW	13	13,669,927 (GRCm38)	missense	probably benign	0.12
R3406:Lyst	UTSW	13	13,635,230 (GRCm38)	missense	possibly damaging	0.56
R3972:Lyst	UTSW	13	13,706,625 (GRCm38)	missense	possibly damaging	0.67
R3978:Lyst	UTSW	13	13,634,168 (GRCm38)	missense	possibly damaging	0.82
R4032:Lyst	UTSW	13	13,616,665 (GRCm38)	missense	probably damaging	1.00
R4192:Lyst	UTSW	13	13,740,513 (GRCm38)	missense	probably damaging	1.00
R4206:Lyst	UTSW	13	13,635,989 (GRCm38)	missense	probably benign	0.03
R4298:Lyst	UTSW	13	13,634,887 (GRCm38)	missense	probably damaging	1.00
R4344:Lyst	UTSW	13	13,698,466 (GRCm38)	missense	probably benign	0.06
R4441:Lyst	UTSW	13	13,635,383 (GRCm38)	missense	probably damaging	1.00
R4445:Lyst	UTSW	13	13,709,564 (GRCm38)	missense	probably benign	0.42
R4477:Lyst	UTSW	13	13,635,383 (GRCm38)	missense	probably damaging	1.00
R4493:Lyst	UTSW	13	13,635,383 (GRCm38)	missense	probably damaging	1.00
R4494:Lyst	UTSW	13	13,635,383 (GRCm38)	missense	probably damaging	1.00
R4495:Lyst	UTSW	13	13,635,383 (GRCm38)	missense	probably damaging	1.00
R4622:Lyst	UTSW	13	13,674,398 (GRCm38)	missense	probably benign	0.01
R4638:Lyst	UTSW	13	13,696,794 (GRCm38)	splice site	probably null
R4658:Lyst	UTSW	13	13,635,383 (GRCm38)	missense	probably damaging	1.00
R4675:Lyst	UTSW	13	13,635,383 (GRCm38)	missense	probably damaging	1.00
R4719:Lyst	UTSW	13	13,650,350 (GRCm38)	missense	probably benign
R4729:Lyst	UTSW	13	13,637,901 (GRCm38)	missense	probably damaging	1.00
R4774:Lyst	UTSW	13	13,740,597 (GRCm38)	missense	probably damaging	1.00
R4811:Lyst	UTSW	13	13,777,100 (GRCm38)	missense	probably benign	0.33
R4877:Lyst	UTSW	13	13,683,149 (GRCm38)	missense	probably damaging	1.00
R4920:Lyst	UTSW	13	13,647,060 (GRCm38)	missense	possibly damaging	0.79
R4933:Lyst	UTSW	13	13,759,378 (GRCm38)	missense	probably benign	0.12
R4933:Lyst	UTSW	13	13,637,764 (GRCm38)	missense	probably damaging	0.98
R4958:Lyst	UTSW	13	13,635,463 (GRCm38)	missense	probably benign	0.00
R4982:Lyst	UTSW	13	13,725,954 (GRCm38)	missense	probably damaging	1.00
R4992:Lyst	UTSW	13	13,661,163 (GRCm38)	missense	probably damaging	1.00
R5024:Lyst	UTSW	13	13,634,404 (GRCm38)	missense	probably benign
R5049:Lyst	UTSW	13	13,636,064 (GRCm38)	missense	probably damaging	1.00
R5079:Lyst	UTSW	13	13,757,353 (GRCm38)	missense	probably benign	0.08
R5254:Lyst	UTSW	13	13,683,070 (GRCm38)	missense	probably benign	0.00
R5266:Lyst	UTSW	13	13,660,970 (GRCm38)	missense	probably damaging	1.00
R5279:Lyst	UTSW	13	13,648,802 (GRCm38)	nonsense	probably null
R5285:Lyst	UTSW	13	13,634,426 (GRCm38)	missense	probably benign	0.01
R5364:Lyst	UTSW	13	13,656,854 (GRCm38)	missense	probably benign	0.35
R5435:Lyst	UTSW	13	13,777,064 (GRCm38)	missense	possibly damaging	0.64
R5516:Lyst	UTSW	13	13,644,122 (GRCm38)	missense	probably benign	0.10
R5524:Lyst	UTSW	13	13,746,779 (GRCm38)	missense	probably benign	0.03
R5591:Lyst	UTSW	13	13,743,333 (GRCm38)	missense	probably damaging	0.99
R5592:Lyst	UTSW	13	13,743,333 (GRCm38)	missense	probably damaging	0.99
R5593:Lyst	UTSW	13	13,743,333 (GRCm38)	missense	probably damaging	0.99
R5594:Lyst	UTSW	13	13,743,333 (GRCm38)	missense	probably damaging	0.99
R5594:Lyst	UTSW	13	13,759,397 (GRCm38)	missense	probably benign	0.00
R5644:Lyst	UTSW	13	13,637,496 (GRCm38)	missense	possibly damaging	0.58
R5659:Lyst	UTSW	13	13,634,627 (GRCm38)	missense	possibly damaging	0.58
R5741:Lyst	UTSW	13	13,634,030 (GRCm38)	missense	probably benign	0.44
R5908:Lyst	UTSW	13	13,696,761 (GRCm38)	nonsense	probably null
R5969:Lyst	UTSW	13	13,687,813 (GRCm38)	splice site	probably null
R6128:Lyst	UTSW	13	13,759,379 (GRCm38)	missense	possibly damaging	0.67
R6271:Lyst	UTSW	13	13,658,754 (GRCm38)	missense	probably benign	0.30
R6315:Lyst	UTSW	13	13,643,504 (GRCm38)	missense	probably benign
R6318:Lyst	UTSW	13	13,743,311 (GRCm38)	missense	possibly damaging	0.88
R6555:Lyst	UTSW	13	13,648,925 (GRCm38)	missense	probably benign	0.01
R6663:Lyst	UTSW	13	13,664,116 (GRCm38)	splice site	probably null
R6701:Lyst	UTSW	13	13,681,485 (GRCm38)	missense	probably benign	0.06
R6711:Lyst	UTSW	13	13,635,235 (GRCm38)	missense	possibly damaging	0.80
R6909:Lyst	UTSW	13	13,743,375 (GRCm38)	missense	probably damaging	1.00
R6915:Lyst	UTSW	13	13,726,044 (GRCm38)	missense	probably benign	0.01
R6929:Lyst	UTSW	13	13,743,324 (GRCm38)	missense	probably damaging	1.00
R6960:Lyst	UTSW	13	13,634,078 (GRCm38)	missense	probably benign	0.12
R7018:Lyst	UTSW	13	13,743,459 (GRCm38)	critical splice donor site	probably null
R7037:Lyst	UTSW	13	13,616,666 (GRCm38)	missense	probably damaging	1.00
R7045:Lyst	UTSW	13	13,637,708 (GRCm38)	missense	probably damaging	1.00
R7045:Lyst	UTSW	13	13,634,900 (GRCm38)	missense	probably benign	0.34
R7070:Lyst	UTSW	13	13,757,444 (GRCm38)	missense	probably benign	0.23
R7188:Lyst	UTSW	13	13,752,090 (GRCm38)	missense	possibly damaging	0.66
R7201:Lyst	UTSW	13	13,709,300 (GRCm38)	nonsense	probably null
R7210:Lyst	UTSW	13	13,656,983 (GRCm38)	missense	probably damaging	1.00
R7229:Lyst	UTSW	13	13,643,509 (GRCm38)	missense	probably benign	0.00
R7293:Lyst	UTSW	13	13,680,237 (GRCm38)	missense	probably benign	0.01
R7318:Lyst	UTSW	13	13,757,443 (GRCm38)	missense	probably benign	0.13
R7344:Lyst	UTSW	13	13,706,555 (GRCm38)	missense	probably benign
R7426:Lyst	UTSW	13	13,637,524 (GRCm38)	missense	probably benign
R7522:Lyst	UTSW	13	13,647,083 (GRCm38)	nonsense	probably null
R7583:Lyst	UTSW	13	13,635,887 (GRCm38)	missense	probably damaging	1.00
R7606:Lyst	UTSW	13	13,637,475 (GRCm38)	missense	probably damaging	1.00
R7636:Lyst	UTSW	13	13,616,747 (GRCm38)	critical splice donor site	probably null
R7658:Lyst	UTSW	13	13,730,476 (GRCm38)	missense	possibly damaging	0.63
R7685:Lyst	UTSW	13	13,669,865 (GRCm38)	missense	probably benign	0.00
R7689:Lyst	UTSW	13	13,683,223 (GRCm38)	critical splice donor site	probably null
R7765:Lyst	UTSW	13	13,709,532 (GRCm38)	missense	possibly damaging	0.75
R7779:Lyst	UTSW	13	13,634,543 (GRCm38)	missense	probably damaging	1.00
R7871:Lyst	UTSW	13	13,636,052 (GRCm38)	nonsense	probably null
R7872:Lyst	UTSW	13	13,635,865 (GRCm38)	missense	probably benign	0.14
R7884:Lyst	UTSW	13	13,707,683 (GRCm38)	missense	probably benign	0.09
R7890:Lyst	UTSW	13	13,740,569 (GRCm38)	missense	probably damaging	0.99
R7916:Lyst	UTSW	13	13,647,072 (GRCm38)	missense	possibly damaging	0.64
R7948:Lyst	UTSW	13	13,746,589 (GRCm38)	missense	possibly damaging	0.59
R7956:Lyst	UTSW	13	13,641,203 (GRCm38)	missense	possibly damaging	0.80
R8048:Lyst	UTSW	13	13,687,645 (GRCm38)	missense	probably benign	0.12
R8085:Lyst	UTSW	13	13,634,309 (GRCm38)	missense	probably damaging	0.98
R8165:Lyst	UTSW	13	13,698,360 (GRCm38)	missense	probably damaging	0.99
R8235:Lyst	UTSW	13	13,760,738 (GRCm38)	missense	possibly damaging	0.69
R8237:Lyst	UTSW	13	13,651,732 (GRCm38)	missense	probably benign	0.00
R8275:Lyst	UTSW	13	13,776,082 (GRCm38)	missense	probably benign	0.02
R8300:Lyst	UTSW	13	13,664,058 (GRCm38)	missense	possibly damaging	0.79
R8350:Lyst	UTSW	13	13,650,388 (GRCm38)	nonsense	probably null
R8526:Lyst	UTSW	13	13,760,806 (GRCm38)	missense	probably damaging	0.99
R8551:Lyst	UTSW	13	13,634,060 (GRCm38)	missense	possibly damaging	0.77
R8723:Lyst	UTSW	13	13,712,757 (GRCm38)	missense	possibly damaging	0.89
R8772:Lyst	UTSW	13	13,637,492 (GRCm38)	nonsense	probably null
R8778:Lyst	UTSW	13	13,728,567 (GRCm38)	missense	possibly damaging	0.89
R8778:Lyst	UTSW	13	13,635,776 (GRCm38)	missense	possibly damaging	0.89
R8801:Lyst	UTSW	13	13,661,010 (GRCm38)	missense	probably benign	0.10
R8837:Lyst	UTSW	13	13,677,963 (GRCm38)	missense	probably benign
R8874:Lyst	UTSW	13	13,637,562 (GRCm38)	missense	probably benign
R8878:Lyst	UTSW	13	13,641,076 (GRCm38)	missense	probably benign	0.00
R8891:Lyst	UTSW	13	13,712,850 (GRCm38)	missense	possibly damaging	0.67
R9077:Lyst	UTSW	13	13,683,108 (GRCm38)	missense	probably benign	0.02
R9127:Lyst	UTSW	13	13,634,242 (GRCm38)	missense	probably damaging	1.00
R9143:Lyst	UTSW	13	13,661,165 (GRCm38)	missense	probably damaging	0.98
R9216:Lyst	UTSW	13	13,648,603 (GRCm38)	missense	probably benign
R9217:Lyst	UTSW	13	13,696,660 (GRCm38)	missense	probably benign	0.01
R9291:Lyst	UTSW	13	13,709,353 (GRCm38)	missense	probably benign	0.01
R9302:Lyst	UTSW	13	13,730,362 (GRCm38)	missense	possibly damaging	0.46
R9370:Lyst	UTSW	13	13,760,748 (GRCm38)	missense	probably damaging	1.00
R9402:Lyst	UTSW	13	13,637,878 (GRCm38)	missense	probably benign
R9457:Lyst	UTSW	13	13,687,745 (GRCm38)	missense	possibly damaging	0.83
R9481:Lyst	UTSW	13	13,683,068 (GRCm38)	missense	possibly damaging	0.68
R9563:Lyst	UTSW	13	13,637,823 (GRCm38)	missense	probably benign	0.36
R9623:Lyst	UTSW	13	13,678,002 (GRCm38)	missense	probably benign
R9661:Lyst	UTSW	13	13,634,194 (GRCm38)	missense	probably benign	0.01
R9682:Lyst	UTSW	13	13,656,941 (GRCm38)	missense	probably benign	0.21
R9743:Lyst	UTSW	13	13,634,738 (GRCm38)	missense	possibly damaging	0.67
R9801:Lyst	UTSW	13	13,634,705 (GRCm38)	missense	probably damaging	0.97
RF001:Lyst	UTSW	13	13,635,841 (GRCm38)	missense	probably benign
RF002:Lyst	UTSW	13	13,634,363 (GRCm38)	missense	probably benign	0.05
X0024:Lyst	UTSW	13	13,634,448 (GRCm38)	missense	probably benign	0.00
X0026:Lyst	UTSW	13	13,751,970 (GRCm38)	missense	probably damaging	0.99
Z1088:Lyst	UTSW	13	13,743,433 (GRCm38)	missense	probably benign	0.09
Z1176:Lyst	UTSW	13	13,777,079 (GRCm38)	missense	probably benign	0.27
Z1176:Lyst	UTSW	13	13,640,107 (GRCm38)	missense	probably damaging	1.00
Z1177:Lyst	UTSW	13	13,680,134 (GRCm38)	missense	possibly damaging	0.73

Predicted Primers

PCR Primer
(F):5'- ACGATGGTCCTTTAGCACCCTCAG -3'
(R):5'- TTTCAGCAAGACTTCTCCAGTCACG -3'

Sequencing Primer
(F):5'- AGAACAGGTTTCAGCCTTTGG -3'
(R):5'- CTCTGTACTGCTAGATAGAATCCACG -3'

Posted On

2014-04-13